Majalah Obat Tradisional最新文献_第8页

Effect of AGE and Gamma-mangostin on Luteinizing Hormone Receptor (LHR) Levels in Leydig Cell Culture of Male Aging Rats: Preliminary Study AGE和γ-芒果苷对雄性衰老大鼠睾丸间质细胞培养中促黄体生成素受体（LHR）水平影响的初步研究

Majalah Obat Tradisional Pub Date : 2021-08-31 DOI: 10.22146/MOT.66827

D. M. Rizal, Muhammad Herdhana Ash Shidiqi, R. Rustamaji

{"title":"Effect of AGE and Gamma-mangostin on Luteinizing Hormone Receptor (LHR) Levels in Leydig Cell Culture of Male Aging Rats: Preliminary Study","authors":"D. M. Rizal, Muhammad Herdhana Ash Shidiqi, R. Rustamaji","doi":"10.22146/MOT.66827","DOIUrl":"https://doi.org/10.22146/MOT.66827","url":null,"abstract":"Advanced Glycation End Products (AGE) are proteins that can cause cell destruction by increasing oxidative stress and inflammation. This product resulted from a series of chemical reactions after an initial glycation reaction. A Leydig cell is one of the types of cells affected by AGE. This cell is located in the interstitial of the testes and stimulated by the luteinizing hormone. This study aimed to compare the luteinizing hormone receptor levels in Leydig cell culture of Sprague-Dawley rat induced by AGE only and the one that administered gamma-mangosteen. We conducted an experimental laboratory study on luteinizing hormone receptor levels in Leydig cell culture of Sprague-Dawley rats induced by advanced glycation end products 200 μg/mL and given gamma-mangostin 5 μM compared to the one that was not given gamma-mangostin. The highest mean of LHR level was in group 3 given gamma-mangostin 5 μM (8.06 pg/ cells/24h), and the lowest mean was in group 1 (control) (7.78 pg/ cells/24h). The LHR levels in the rats' Leydig cell culture given 5μM gamma-mangostin were higher than the other groups indicate the inhibition capacity on the oxidation process caused by AGE in aging rats Leydig cells culture.","PeriodicalId":32438,"journal":{"name":"Majalah Obat Tradisional","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49309846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Ozonated Olive Oil as Adjunctive Therapy after Periodontal Pocket Curettage towards Collagen Density of Alveolar Bone in Periodontitis Healing Process (In Vivo Study with Sprague dawley) 臭氧化橄榄油在牙周炎愈合过程中辅助治疗对牙槽骨胶原密度的影响(与Sprague dawley的体内研究)

Majalah Obat Tradisional Pub Date : 2021-08-31 DOI: 10.22146/MOT.65984

D. Herawati, Etty Cahya Pertiwi

{"title":"Application of Ozonated Olive Oil as Adjunctive Therapy after Periodontal Pocket Curettage towards Collagen Density of Alveolar Bone in Periodontitis Healing Process (In Vivo Study with Sprague dawley)","authors":"D. Herawati, Etty Cahya Pertiwi","doi":"10.22146/MOT.65984","DOIUrl":"https://doi.org/10.22146/MOT.65984","url":null,"abstract":"Periodontitis is an inflammatory disease of the supporting tissue of the teeth caused by specific microorganisms or groups of specific microorganisms, resulting in progressive destruction of the periodontal ligament and alveolar bone with periodontal pocket formation. Application of ozonated olive oil in dentistry is based on the actions such as antimicrobial and therapeutic agent, needed as adjunctive therapy after periodontal pocket curretage. Collagen is the main constituent of alveolar bone extracellular matrix and needed as a scaffold in the formation of mineralized matrix. The aim of this study was to determine the density of collagen in alveolar bone on periodontitis healing process after adjunctive topical application of ozonated olive oil in periodontal pocket curretage. In this study, 32 Sprague dawley rats were randomly divided into two groups: curetted-topical application of ozonated olive oil as treatment groups and curetted-topical application of 1% CMC-Na as placebo group. Periodontitis induced by placing silk-ligature around submandibular incisors for 7 days. Subsequently, the rats were sacrificed on days 3, 5, 7, and 14 after curetted and topical application, and each group was represented by four rats. The staining was done using Mallory staining method. All the results were statistically analyzed using Kruskal-Wallis and Mann-Whitney tests. The results showed that there were significant differences (p<0,05) in the density of collagen between two groups. The study concluded that adjunctive topical application of ozonated olive oil after periodontal pocket curretage significantly increase the density of collagen in alveolar bone on periodontitis healing process in Spraque dawley.","PeriodicalId":32438,"journal":{"name":"Majalah Obat Tradisional","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42671728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accelerated Stability Test of Snakehead Fish Extract (Channa striata) and Kelulut Honey (heterotrigona itama) Ointment Combination with Tween 80 and Span 80 as an Emulsifying Agent 以Tween 80和Span 80为乳化剂的黑头鱼提取物和海露蜂蜜软膏的加速稳定性试验

Majalah Obat Tradisional Pub Date : 2021-08-31 DOI: 10.22146/MOT.66608

Wintari Taurina, Mohamad Andrie, Taufik Nanda Pratama

{"title":"Accelerated Stability Test of Snakehead Fish Extract (Channa striata) and Kelulut Honey (heterotrigona itama) Ointment Combination with Tween 80 and Span 80 as an Emulsifying Agent","authors":"Wintari Taurina, Mohamad Andrie, Taufik Nanda Pratama","doi":"10.22146/MOT.66608","DOIUrl":"https://doi.org/10.22146/MOT.66608","url":null,"abstract":"The combination of Snakehead Fish and Kelulut Honey has activity in accelerating the wound healing process. The ointment with active ingredients of Snakehead Fish extract and Kelulut Honey showed separation from the active ingredient and its base. The addition of tween 80 and span 80 is expected to increase the stability of the ointment preparation by keeping the water-phase droplets distributed uniformly on the ointment base. This study aimed to determine the effect of adding Tween 80 and Span 80 on the stability of the ointment preparation. The ointment was made by varying Tween 80 and span 80 by 2,5%: 5%: 7,5% and control (active ingredients without the addition of tween 80 and Span 80) as a comparison. The preparation was subjected to an accelerated stability test using a temperature of 40 ° ± 2 ° C / Relative humidity 75% ± 5% for 28 days by observing the organoleptic test, adhesion, spreadability, homogeneity test, pH test, and protection power. The data obtained were analyzed statistically using One Way Anova. The analysis showed that the addition of tween 80 and span 80 with a total concentration of 5% and 7,5% had a significant effect in increasing the spreadability, decreasing adhesion, and reducing the pH of the preparation. Formulas that meet the requirements in this study are F2 and F3, which have an average spreadability of 5,11 cm and 5,25 cm, adhesion of 96 seconds and 51 seconds, and pH of 6,02 and 5,02.","PeriodicalId":32438,"journal":{"name":"Majalah Obat Tradisional","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45443681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In Vitro ACE Inhibitory Activity and Bioactive Compounds of Aqueous Extract of Citrus amblycarpa 水提物体外ACE抑制活性及活性成分研究

Majalah Obat Tradisional Pub Date : 2021-08-31 DOI: 10.22146/MOT.65028

I. Kusumawati, I. Putra, Ida Bagus Agung Yogeswara

引用次数: 3

The Effects of Green Betel Leaf (Piper betle) Extract Eye Drops on the Number of Staphylococcus aureus Colonies in Conjunctivitis Wistar Rats Model (Rattus novergicus) 槟榔叶提取物滴眼液对结膜炎Wistar模型大鼠金黄色葡萄球菌菌落数量的影响

Majalah Obat Tradisional Pub Date : 2021-08-31 DOI: 10.22146/MOT.65242

Mahira Aisyah Putri Nur, S. Sudaryanto, E. Lestari, Erwin Kresnoadi

{"title":"The Effects of Green Betel Leaf (Piper betle) Extract Eye Drops on the Number of Staphylococcus aureus Colonies in Conjunctivitis Wistar Rats Model (Rattus novergicus)","authors":"Mahira Aisyah Putri Nur, S. Sudaryanto, E. Lestari, Erwin Kresnoadi","doi":"10.22146/MOT.65242","DOIUrl":"https://doi.org/10.22146/MOT.65242","url":null,"abstract":"Irrational use of antibiotics can cause resistance to some diseases such as bacterial conjunctivitis caused by Staphylococcus aureus. Previous research said that green betel leaf (Piper betle) contains antimicrobial compounds such as eugenol, cavalry, tannins, saponins, and flavonoids which also have antiseptic power as well as antibiotics. This study aims to prove the decrease in the number of Staphylococcus aureus colonies after giving green betel leaf eye drops (Piper betle) to conjunctivitis Wistar rats (Rattus norvegicus). This research was true experimental with a pretest-posttest control group design. 25 male white Wistar rats as objects were selected by simple random sampling and grouped into 5 groups randomly. The rats were given Staphylococcus aureus, control group K+ was given 0.5% levofloxacin eye drops, and K- was given aquadest as a comparison. Treatment groups P1, P2, and P3 were given different concentrations of betel leaf eye drops. Swab pretest was done 3 days after the rats were inoculated with Staphylococcus aureus and swab post-test was done 5 days after the rats were given green betel leaf eye drops, the calculation of Staphylococcus aureus bacteria colonies used the Total Plate Count (TPC) method. There was a significant decrease (p<0.05) in the number of bacterial colonies for K-, K+, P1, P2, and P3 groups. In addition, there was a significant difference (p<0.05) between K+ with P1 and K+ with P3. There was a decrease in the number of Staphylococcus aureus for all groups of conjunctivitis Wistar rats (Rattus norvegicus)","PeriodicalId":32438,"journal":{"name":"Majalah Obat Tradisional","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42219353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ethanolic Extract of Pluchea indica Less leaf Increases Serum Growth Hormone in Lactating Rats 梅子叶乙醇提取物提高泌乳大鼠血清生长激素水平

Majalah Obat Tradisional Pub Date : 2021-08-31 DOI: 10.22146/MOT.62060

Rul Afiyah Syarif, N. Anggorowati, Mia Munawaroh, M. Wahyuningsih

{"title":"Ethanolic Extract of Pluchea indica Less leaf Increases Serum Growth Hormone in Lactating Rats","authors":"Rul Afiyah Syarif, N. Anggorowati, Mia Munawaroh, M. Wahyuningsih","doi":"10.22146/MOT.62060","DOIUrl":"https://doi.org/10.22146/MOT.62060","url":null,"abstract":"The objectives of the study were to evaluate the effect of ethanolic extract of P. indica Less leaf (EPI) on serum growth hormone (GH), milk yield, body weight gain of dams, and dam’s organ weight in lactating rats. A total of 25 lactating rats with six pups were randomized and distributed to one of five treatments (control (RO water), standard (domperidone 2,5 mg/kg BW/day), EPI 250 (250 mg/kg BW of EPI), EPI 500 (500 mg/kg BW of EPI), and EPI 750 (750 mg/kg BW of EPI)). The treatment was administered daily starting from the 2nd until the 15th day of lactation. On the 16th day serum growth hormone level, body and organ weight of rats were measured. Serum GH levels in the EPI 750 group (1963.25 ± 360.91 pg/µL) increased significantly compared to the domperidone (409.46 ± 28.80 pg/µL) and the control group (723.40 ± 95.78 pg/µL, p0.05). There was significant body weight gain in the EPI 750 group compared with the domperidone group. There was no significant difference in organ weight gain in each group. The study revealed that ethanolic extract of P. indica Less leaf can increase serum growth hormone in lactating rats.","PeriodicalId":32438,"journal":{"name":"Majalah Obat Tradisional","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45195471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antioxidants and Antityrosinase Activity of Ethanolic Basil Leaves Extract (Ocimum americanum L.) and Eygenol 罗勒叶乙醇提取物和Eygenol的抗氧化活性及抗酪氨酸酶活性

Majalah Obat Tradisional Pub Date : 2021-08-31 DOI: 10.22146/MOT.56081

Dio Damara Handoyo, E. Girsang, A. N. Nasution, N. Lister

引用次数: 2

Hydroxyl Radical Scavenging Activity of Stachytarpheta jamaecensis Root Extract using In Vitro Deoxyribose Degradation Assay 用体外脱氧核糖降解法测定牙买加水藻根提取物的清除羟基自由基活性

Majalah Obat Tradisional Pub Date : 2021-08-31 DOI: 10.22146/MOT.61746

J. P. Utami, Yusrinie Wasiaturrahmah, Deby Kania Tri Putri

{"title":"Hydroxyl Radical Scavenging Activity of Stachytarpheta jamaecensis Root Extract using In Vitro Deoxyribose Degradation Assay","authors":"J. P. Utami, Yusrinie Wasiaturrahmah, Deby Kania Tri Putri","doi":"10.22146/MOT.61746","DOIUrl":"https://doi.org/10.22146/MOT.61746","url":null,"abstract":"Use and demand of traditional medicinal plants currently growing in the direction that research in traditional medicines have also increased. One of the plants that is efficacious as a medicine is S. jamaecensis. This plant is usually used by people as a traditional medicine to treat various diseases. This study aims to determine the antioxidant activity of porterweed root. Antioxidant activity assays performed using hydroxyl radical scavenger that begins with the extraction by maceration. Dry roots of S. jamaecensis (250 g) was soaked with ethanol 96% for 3x24 hours at room temperature. The liquid extract obtained is evaporated by rotary evaporator and waterbath till viscous extracts is obtained. Then to extract performed phytochemical screening by quantitative and qualitative method. The antioxidant test was carried out using the hydroxyl radical scavenger method. The extract concentrations were varied, namely 125, 250, 500 and 1000 ppm. The data obtained was performed one-way ANOVA test. The results of the phytochemical screening showed the extract contains flavonoids, alcaloids, saponins, phenols and tannins. The results obtained were significant values from the antioxidant test with the antiradical scavenger method, the largest average value of root extract was 60.606% at a concentration of 1000 ppm. The results of spectrophotometric measurements showed that the extract has IC50 was 683.5294 µg/ml, while vitamin C has IC50 lower value (251.700 µg/ml). Q","PeriodicalId":32438,"journal":{"name":"Majalah Obat Tradisional","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49587428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radical Scavenging Activity and Acute Toxicity of Bitter Melon (Momordica Charantia L.) Seed Oil 苦瓜自由基清除活性及急性毒性研究籽油

Majalah Obat Tradisional Pub Date : 2021-04-07 DOI: 10.22146/MOT.52743

Lina Winarti, Lusi Kumalasari, Evi Umayah Ulfa

{"title":"Radical Scavenging Activity and Acute Toxicity of Bitter Melon (Momordica Charantia L.) Seed Oil","authors":"Lina Winarti, Lusi Kumalasari, Evi Umayah Ulfa","doi":"10.22146/MOT.52743","DOIUrl":"https://doi.org/10.22146/MOT.52743","url":null,"abstract":"Bitter Gourd or bitter melon (Momordica Charantia L.) is a common type of vegetable and safe for daily consumption. The seeds are part of bitter gourd that useless. Research on bitter melon seed oil has promising commercial applications. This study aims to determine the antioxidant potential and safety of bitter melon seed oil through acute toxicity study. The content of phenols, flavonoids, and antioxidant activity was analyzed. The antioxidant activity was evaluated using the DPPH method. Antioxidant activity is expressed as an IC-50 value. The results showed that the phenol content of bitter melon seed oil was 0.0118 ±0.0006%, the flavonoid content was 0.0127±0.0004%. From the antioxidant activity study, the IC-50 of vitamin C was 2.41 μg / ml, while an IC-50 of bitter melon seed oil was 11.31 ± 0.77 mg/ml. The results of this antioxidant activity study showed very weak activity. The results of the acute-toxicity study show LD-50 cannot be determined precisely because up to the highest dose of 100 ml/kg does not cause death even though it causes toxic symptoms such as diarrhea. Overall, test results indicate that bitter melon seed oil is a compound that is categorized as practically non-toxic with low antioxidant activity.","PeriodicalId":32438,"journal":{"name":"Majalah Obat Tradisional","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47437357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and Prediction of Potential Compounds from Virgin Olive Oil Acting on Proteins Associated with Cancer Disease 初榨橄榄油中作用于癌症相关蛋白的潜在化合物的筛选和预测

Majalah Obat Tradisional Pub Date : 2021-04-07 DOI: 10.22146/MOT.52737

Achmad Rodiansyah

引用次数: 0