THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS最新文献_第5页

Vitamin B12 Deficiency in type I Diabetes I型糖尿病患者缺乏维生素B12

THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS Pub Date : 2020-08-26 DOI: 10.52700/jmmg.v1i2.19

Sana Ashiq, Shabana

{"title":"Vitamin B12 Deficiency in type I Diabetes","authors":"Sana Ashiq, Shabana","doi":"10.52700/jmmg.v1i2.19","DOIUrl":"https://doi.org/10.52700/jmmg.v1i2.19","url":null,"abstract":"Diabetes mellitus is a group of metabolic anomalies due to defect in insulin secretion/action. In type I diabetes, there is complete absence of insulin due to destruction of pancreatic beta cells, whereas type 2 diabetes usually involves insulin resistance. A complex interaction between various environmental, genetic and immunological factors leads to development of diabetes. One major problem for treating T1DM is its difficult prognosis because it leads to many complications, including autoimmune gastritis and celiac diseases. In autoimmune gastritis auto/parietal cell antibodies are formed against the intrinsic factor leading to thegastric atrophy. While in celiac disease, intestinal villi atrophy occurs. Both these conditions have a common clinical outcome which is pernicious anemia. The patients suffering from type I diabetes have approximately threefold increased risk for developing vitamin B12 deficiency as compared to the general population. The major genetic risk factor responsible for this disease is any functional change in HLA class II alleles (HLA DQ, DR and DP). These genes are present on chromosome 6 and are involved in approximately 40% cases of type I diabetes mellitus. The production of glutamic acid decarboxylase and anti-thyroid peroxidase antibodies have also been reported to be associated with this genotype. The glutamic acid decarboxylase converts glutamic acid to gamma-amino butyric acid producing hydrochloric acid from gastric parietal cells. The antibodies against this antigen can cause achlorhydria that can ultimately lead to vitamin B12 deficiency. Hematologic and neurological disorders, as well as a variety of cardiovascular diseases, are the most commonclinical manifestations of deficiency of vitamin B12.","PeriodicalId":322027,"journal":{"name":"THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122240328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety of Street Foods in Developing Countries: An overview from Pakistan 发展中国家的街头食品安全:来自巴基斯坦的综述

THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS Pub Date : 2020-04-30 DOI: 10.52700/jmmg.v1i3.8

Iram Asim, H. Yasmeen

引用次数: 0

Imidacloprid Degradation Potential Of Bacillus Strain Isolated From Agriculture Soil 农业土壤中分离的芽孢杆菌对吡虫啉的降解潜力

THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS Pub Date : 2020-04-24 DOI: 10.52700/jmmg.v1i1.5

Ayesha Naseer, Shumaila Nazuk, A. Iqbal

{"title":"Imidacloprid Degradation Potential Of Bacillus Strain Isolated From Agriculture Soil","authors":"Ayesha Naseer, Shumaila Nazuk, A. Iqbal","doi":"10.52700/jmmg.v1i1.5","DOIUrl":"https://doi.org/10.52700/jmmg.v1i1.5","url":null,"abstract":"Continuous dispersion of pesticide in the environment and its stability in soil results in environmental pollution that cause harmful effect to living organisms and human by entering in food chain and this hazardous problem need to eradicate by remediation. The present research was attempted to isolate and characterize imidacloprid (IMIDA) degrading Bacillus strains from the different areas of Multan region. The imidacloprid resistant strains were isolated by checking their imidacloprid degradation ability in minimal salt medium (MSM). Levels of imidacloprid in MSM medium were analyzed by thin layer chromatography (TLC) and Bacillus mediated degradation of imidacloprid was assessed by using fourier transform infrared spectroscopy (FTIR). Out of 38 imidacloprid resistant strains, from 6 different soil samples, A2 showed highest imidacloprid degrading ability. The Bacillus strain A2 showed maximum growth at 25°C and pH 7 (at 600 nm). Degradation of IMIDA was determined by Rf values for IMIDA containing control (0.72) and culture extracts (0.8, 0.54 and 0.3) in TLC analysis. FTIR spectrum of strain A2 showed the appearance of new peaks at 2253 cm-1 from 2500 to 2000 cm-1 peak area as compared to control. The shifts of the peak pattern as well as occurrence of few new peaks attribute to biodegradation of imidacloprid after bacterial treatment. This study has shown that soil microbial activity significantly affected the technical profile of imidacloprid insecticides after soil application.","PeriodicalId":322027,"journal":{"name":"THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116590525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Use Of Baceriocin Producing Lactobacilli Strains From Fruits And Vegetables In Food Preservation 产杆菌素的果蔬乳杆菌菌株在食品保鲜中的应用

THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS Pub Date : 2020-04-24 DOI: 10.52700/jmmg.v1i1.4

Syeda Fatima Zehra, Umaima Rubbani, A. Iqbal

{"title":"Use Of Baceriocin Producing Lactobacilli Strains From Fruits And Vegetables In Food Preservation","authors":"Syeda Fatima Zehra, Umaima Rubbani, A. Iqbal","doi":"10.52700/jmmg.v1i1.4","DOIUrl":"https://doi.org/10.52700/jmmg.v1i1.4","url":null,"abstract":"Bacteriocin producing bacteria plays an important role in food preservation. The bacteriocin producing Lactobacilli strains were isolated from different fruits and vegetables from the local markets of Punjab, Pakistan. The bacterial were grown on MRS selective media and their biochemical activity was checked further for the confirmation of Lactobacilli. Antimicrobial activity of these selected strains was checked against different pathogens. For further characterization of bacteriocin production by Lactobacilli, the activity of bacteriocin at different parameters such as at different temperature and pH and the proteolytic activity were checked. Antibiotic activity of bacteriocin producing Lactobacilli was also checked against ampicillin, ciprofloxacin, streptomycin and tetracycline. Out of total strains isolated, 10 strains were selected as Lactobacilli on the basis of their morphology and biochemical characterization. 80% of strains showed zone of inhibition with the best zone being shown by Ct2 (14 mm) against E.coli (ETCC) and A3 (14 mm) against Enterobacteria. Bacteriocin ability of these selected strains was found maximum at 7 pH and 35ºC. The strain T1 was sensitive to ampicillin, ciprofloxacin and streptomycin and strain T2 was resistant to ciprofloxacin, streptomycin and tetracycline. The best characterized strains were selected for fruit juice preservation where lactobacilli producing bacteriocin prevent the growth of other food spoiling bacteria.","PeriodicalId":322027,"journal":{"name":"THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116391099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential Detergent Compatibility And De-staining Ability Of Cellulolytic Bacteria 纤维素分解菌的去污剂相容性及去污能力

THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS Pub Date : 2020-04-22 DOI: 10.52700/jmmg.v1i1.3

A. Iqbal, Mahnoor

{"title":"Potential Detergent Compatibility And De-staining Ability Of Cellulolytic Bacteria","authors":"A. Iqbal, Mahnoor","doi":"10.52700/jmmg.v1i1.3","DOIUrl":"https://doi.org/10.52700/jmmg.v1i1.3","url":null,"abstract":"Cellulases are inducible enzymes that are synthesized by many microorganisms during their growth on cellulosic materials. Production of cellulase enzyme from bacteria has gained interest for applications in industries owing to their stability, catalytic activity and ease of production. In current study, total 40 cellulolytic bacteria were screened by agar well diffusion assay followed by Congo red stain. Cellulolytic bacteria were evaluated for detergent compatibility and de-staining ability. Bacterial cellulase production was optimized at different environmental conditions. Biochemical testing was done following Bergey’s manual. Ten cellulolytic bacteria selected, were gram positive. Bacteria showed best cellulolytic activity at 40oC to 60oC, at pH 9, lactose as carbon source and peptone as nitrogen source. Best hydrolysis zone shown was 45mm from strain MS22 and least zone was 6mm. The maximum detergent activity for surf excel was observed for G2 while the lowest was observed for MW22. Similarly, maximum activity for ariel and bonus were observed for RS5 and C3. G1 and C3 showed better clearance of ink as compared to other strains. In de-staining, G1, C1and MW17 showed better clearance of edible oil and G1, C2 and MW18 showed better clearance of grease. These promising cellulolytic bacteria can be used for various applications in different industries.","PeriodicalId":322027,"journal":{"name":"THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122114651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0