Microbial Biosystems Journal最新文献

{"title":"Assessment of diesel degrading potential of fungal and bacterial isolates from Egypt","authors":"MT Mohesien, E. El-Morsy, M. Abou-Dobara","doi":"10.21608/mb.2018.26272","DOIUrl":"https://doi.org/10.21608/mb.2018.26272","url":null,"abstract":"Two naturally occurring fungal and bacterial species, Aspergillus flavus and Bacillus sp. H6 strains, were capable of utilizing diesel oil as a sole source of carbon in synthetic microcosoms. The initial diesel oil contamination of 1666 mg kg dry soil was reduced to 166.667 mg kg after 150 days of incubation in fungal-bacterial consortium microcosm. That is mean 89.9%, of the initial oil concentration was removed. Abiotic process reduced the diesel oil contamination to about 616 mg kgdry soil at the end of the experiment. Seven microcosms were set up to fulfill the experiments. The decontamination activity follow this order; Bacillus sp. H6 + A. flavus consortium > Bacillus sp. H6 > natural control > A. flavus > cycloheximide treated > benzyl Penicillin-Streptomycin treated > poisoned control. Gas chromatographic analysis data revealed that both A. flavus and Bacillus sp. H6 treatment led to complete utilization of carbon-17 compounds. Other biodegradation products such as C-15, 16, 20, 21, and 24 appears in the chromatogram after 150 days incubation. Increase of C-20, 21 and C-24 compounds also noticed. The fungaland bacterium consortium treatment depicted a decrease of all detected n-alkanes. The microbial success in biodegradation was evaluated by determining the number of germinating seeds of Phaseolus vulgaris. The highest level of germination (92 %) was detected in consortium microcosms after 150 days incubation. The treatment with A. flavus and Bacillus sp. H6 separately led to a lower percentage of germination (86%). The other treatments showed variable results except the poisoned control that showed negative germination and minor chemical degradation of diesel oil. Thus, bacterial-fungal consortium treatment is effective in bioremediation of contaminated oils than separate treatment.","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"253 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79443071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical Profile and Lactic Acid Bacteria Genera Inhabit Egyptian Raw Camel, Sheep, Goat, Buffalo and Cow milks","authors":"A. Darwish, M. Allam, E. Ayad","doi":"10.21608/mb.2018.4276.1001","DOIUrl":"https://doi.org/10.21608/mb.2018.4276.1001","url":null,"abstract":"","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82723091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of some insensitive probiotic lactic acid bacteria and ginger as functional dairy products","authors":"Allam Mg, Gomaa Mae, Ayad Ehe, Darwish Sm","doi":"10.21608/MB.2018.12360","DOIUrl":"https://doi.org/10.21608/MB.2018.12360","url":null,"abstract":"The use of dairy-based functional foods has increased markedly over the last fewyears. Ginger is believed to exert a wide range of therapeutic properties. This study aimed toevaluate probiotic properties of twenty Lactic acid bacteria to apply in functional fermented milkfortified with fresh ginger juice to increase the therapeutic and nutritional effects of the product.Ginger juice was prepared from ginger rhizomes through sorting, washing, peeling, crushing andcrude juice extraction. It was assayed for antibacterial activity by minimal inhibitoryconcentration against antibiotic resistant pathogenic bacteria (E. coli BA 12296, Bacillus subtilisDB100, Klebsiella pneumoniaa ATCC12296, Salmonella senftenberg ATCC 8400,Staphylococcus aureus NCTC 10788, Staphylococcus epidermidis ATCC 35984). Total phenol,total flavonoids and antioxidant activity were determined, ginger juice showed an antibacterialactivity and antioxidant activity. Twelve strains out of twenty were resistant to bile saltsconcentrations (0.2, 0.3 and 0.4 w/v), to acid conditions (pH 2.0, 3.0 and 4.0) and were able togrow in the presence of 0.2 and 0.4 w/v of phenol. Seven strains (Lactobacillus delbrueckiisubsp. delbrueckii KT615, Lb. brevis KP653, Lb. delbrueckii subsp. lactis KP645, Lb. plantarumKP623, Lb. paracasei subsp. tolerans WT631, Enterococcus faecalis BM711, Enterococcusfaecium BT734) had the ability to adhere to rabbit intestinal epithelial cells in-vitro, and wereresistant to ginger juice. Ginger juice concentration showed indirect relationship with the milkcoagulation time and direct with syneresis of fermented milk. It was showed that used 2% ofginger juice concentrations was the best results on fermented milk products.","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"1076 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76694044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional attributes of ethnically edible ectomycorrhizal wild mushroom Amanita in India","authors":"Greeshma Aa, Sridhar Kr, M. Pavithra","doi":"10.21608/MB.2018.12358","DOIUrl":"https://doi.org/10.21608/MB.2018.12358","url":null,"abstract":"d products. This study evaluates functional properties of an ectomycorrhizal wildmushroom Amanita sp. occurring in the lateritic scrub jungles of southwestern India. Based onthe ethnic knowledge, immature cooked fruit bodies of this mushroom are edible. Standardprotocols were followed to evaluate functional properties of uncooked and cooked immaturefruit bodies (pH-dependent protein solubility; least gelation concentration; water- and oilabsorptioncapacities; emulsion and foam properties). The protein solubility was significantlyhigher in uncooked against cooked samples (pH 2-8, p 0.05) despite water-absorption and oil-absorption capacities were higher in cookedsamples. The emulsion activity (p 0.05) were higher in cooked than uncooked samples. The PrincipalComponent Analysis (PCA) between proximal and functional properties reveals that the crudeprotein, total lipids and crude fibre influenced the protein-solubility, emulsion stability and foamcapacity in uncooked samples. In cooked samples, the crude fibre and carbohydrates influencedall the functional properties studied except for protein solubility. It is assumed that thecomposition and proportion of proximal components influence the functional attributes ofAmanita sp. The properties like high emulsion activity, emulsion stability and foam capacity incooked mushroom will be useful in formulation of value-added foods or nutraceutical products.","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74087059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodiversity of macrofungi in Yenepoya Campus, Southwest India","authors":"Karun Nc, Bhagya Bs, Sridhar Kr","doi":"10.21608/MB.2018.12354","DOIUrl":"https://doi.org/10.21608/MB.2018.12354","url":null,"abstract":"Public and private institutions usually possess land space for buildings, play grounds,gardens, avenues and natural vegetation. Such landscapes possess diverse flora, fauna andmicrobiota. Inventory of biodiversity in the vicinity constitutes first step to follow the status ofhabitat based on diversity, carrying capacity and further steps for conservation or rehabilitation.The current study assessed macrofungal diversity in the Yenepoya Campus of southwest India incontinuation of assessment of flora and fauna. This preliminary inventory was carried out in 10habitats (three plantations, bamboo thickets, gardens, acacia groves, avenue trees, lawns, dumpedwood and termite mounds), which yielded 40 species of macrofungi belonging to 31 genera.Among them, twelve species were edible, twelve species were medicinal, two species wereectomycorrhizal and finally one species was entomophagous. Substrates supporting macrofungiinclude soil (particolous), humus (humicolous), woody debris (lignicolous) and insects(entomophagous). Results showed an abundance of five species (Amylosprous campbellii,Daldinia concentrica, Lenzites betulina, Marasmiellus stenophyllus and Schizophyllumcommune), 13 others were common and 22 species were occasional. In addition to flora andfauna, inventory of saprophytic macrofungi (involved in recycling the organic matter) occurringin an institution surroundings help designating the habitat as healthy or regenerated orimpoverished to follow appropriate measures to maintain the status quo, rehabilitation andconservation. This study suggests possibilities of domestication and utilization of several edible,medicinal and ectomycorrhizal fungi in this area.","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76544325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Checklist of Algerian fungi – Part 1: Protozoan Fungal Analogues (Myxomycetes)","authors":"S. Amrani, Abdel-Azeem Am","doi":"10.21608/MB.2018.12359","DOIUrl":"https://doi.org/10.21608/MB.2018.12359","url":null,"abstract":"Informations on Algerian mycobiota are scattered through a wide array of journals,books, dissertations and the lack of comprehensive catalogs or checklists makes difficult toapprehend it diversity. By screening all available bibliographic sources and herbaria catalogues itwas possible to delineate 97 species belonging to 31 genera and from which 9 species have notbeen yet reported in the literature for Algeria. This checklist is the first comprehensive specieslist fully dedicated to Algerian Protozoan Fungal Analogues.","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86776016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical profile and Lactic Acid Bacteria genera inhabit Egyptian raw camel, sheep, goat, buffalo and cow","authors":"Darwish Amg, Allam Mg, Ayad Ehe","doi":"10.21608/mb.2018.12355","DOIUrl":"https://doi.org/10.21608/mb.2018.12355","url":null,"abstract":"Indigenous dairy products made of different milk sources are traditionally produced and consumed in a majority of African and Arabian countries. The aim of present study was to assess and compare physicochemical profile of camel, sheep, goat, buffalo and cow’s native milks in addition to isolation, identification and technological evaluation of their content of Lactic Acid Bacteria (LAB) genera to introduce a wider image that facilitate applications of these pasteurized milks along with isolated LAB strains in dairy industry development. Milks were examined chemically and via Fourier Transform Infrared (FTIR) spectroscopy. Sixty strains out of two hundred sixty-eight of LAB were selected based on assessment of their technological properties. Chemically, sheep and buffalo milks showed the highest content of protein, fat and ash. Goat and sheep milks possessed high content of lactose, which encouraged the inhabitance of Leuconostoc sp. to represented 48% and 18% respectively of their total LAB isolates. Some strains showed exceptional autolytic, photolytic and antimicrobial features (10, 15 and 12 strains respectively). Obtained findings when correctly applied would lead to develop an innovative dairy processing in Egypt. Key words– FTIR, Functional properties, LAB Isolation, Native row milks, Phenotypic identification; Proximate analysis. Introduction Milk as nature’s most complete food, considered daily source of nutrients in most countries. Studies on milk composition of dairy animals mainly concerned cow milk (85% of consumed milk), a lesser extent on goat and sheep milk, and rather rare studies on buffalo and camel in spite of their nutritional importance (Konuspayeva et al. 2009). Cow milk is the most universal raw material of manufactured dairy products (Dandare et al. 2014). Buffalo’s milk is ranked second in the world after cow’s milk (Ahmad et al. 2008). Camel milks play a major role and they are either home-consumed or sold (Yam et al. 2015). Sheep milk contains higher levels of total solids and major nutrient than other milks that affect coagulation time and rate, curd firmness, and amount of rennet used (Park et al. 2007). In recent years, with the huge issue in relation to green analytical technique, some scientist try to used environmentally friendly techniques. The FTIR technique has been gaining Microbial Biosystems 3(1): 12–24 (2018) ISSN 2357-0334 http://fungiofegypt.com/Journal/index.html Microbial Biosystems Copyright © 2018 Darwish et al. Online Edition","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"243 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82759874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saving the forgotten kingdom in Malta","authors":"Lewis M","doi":"10.21608/mb.2018.12363","DOIUrl":"https://doi.org/10.21608/mb.2018.12363","url":null,"abstract":"","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77778703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal activity of some toothpastes and antiseptic solutions against yeasts isolated from the oral cavity of cancer radiated patients","authors":"Attia Ea","doi":"10.21608/MB.2018.12357","DOIUrl":"https://doi.org/10.21608/MB.2018.12357","url":null,"abstract":"This study aimed to investigate the antifungal efficacy of different types of toothpaste and some antiseptic solutions with varying concentrations against yeast isolated from the oral cavity of radiated patients suffering from head and neck cancer in radiotherapy unit in Suez Canal University teaching’s hospital, Egypt. Eight different species of yeast were isolated taxonomically, assigned to 1 phylum, two classes, one order, and three families and identified phenotypically. The antifungal activity of five kinds of toothpaste and three antiseptic solutions were investigated by agar well diffusion method against the eight isolated yeasts namely: Candida glabrata, C. albicans, C. tropicalis, C. kefyr, C. guilliermondii, C. parapsilosis, Dipodascus geotrichum and Geotrichum beigelii and expressed as the value of the clear zone diameter, however, Their activity was expressed by minimum inhibitory concentration (MIC). Results showed that toothpaste no 1, 3, 4 and 5 were effective against all tested taxa by variable inhibitory degrees. However, no. 2 showed efficacy only against C. glabrata, C. guilliermondii, and C. parapsilosis, Antiseptic solution no 1 showed the highest efficacy against all tested species except C. kefyr while no. 2 was effective against all the tested species except two taxa and finally no. 3 exhibited good antifungal activity against all the tested taxa. In conclusion, we recommended the use of toothpaste contained sodium monofluorophosphate, or sodium fluoride, as an active component followed by a mouthwash which contained Chlorohexidine or Cetylpridinium or miswak as active components to prevent colonization and development of oral candidiasis, especially in cancer patients. Key words– Candida, efficacy, Dipodascus geotrichum, Geotrichum beigelii, Suez Canal University. Introduction The oral cavity is a habitat for a large number of microbiota which coexists with another as a normal microbiome. However, Candida species are ovoid budding yeast-like fungi found as a normal commensal in the oral cavity, vagina, and skin and throughout the gastrointestinal tract of humans. Some factors leading to increased incidence of oral thrush and candidiasis as poor oral hygiene, nutritional deficiencies, high carbohydrate diet, diabetes mellitus, heavy cigarette smoking, dental prostheses, treatment by radiotherapy, immunosuppression, and HIV infection (Meghana et al. 2015). Thus the changes occur in mechanisms of host defense, and the buccal Microbial Biosystems 3(1): 25–33 (2018) ISSN 2357-0334 http://fungiofegypt.com/Journal/index.html Microbial Biosystems Copyright © 2018 Attia Online Edition","PeriodicalId":32201,"journal":{"name":"Microbial Biosystems Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86780149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}