Journal of applied oral science : revista FOB最新文献

{"title":"Comparison of rRNA-based reverse transcription PCR and rDNA-based PCR for the detection of streptococci in root canal infections.","authors":"Laís Cunha Prado,&nbsp;Giulio Gavini,&nbsp;Amanda da Costa Silveira,&nbsp;Vitor Cesar Nakamura,&nbsp;Marcia Pinto Alves Mayer,&nbsp;Ericka Tavares Pinheiro","doi":"10.1590/1678-7757-2018-0256","DOIUrl":"https://doi.org/10.1590/1678-7757-2018-0256","url":null,"abstract":"<p><strong>Objective: </strong>The rDNA-based method is unable to distinguish between alive and dead cells. Alternatively, bacterial viability can be assessed by molecular methods based on ribosomal RNA (rRNA). Therefore, this study aimed to detect viable streptococci in root canal samples using rRNA-based reverse transcription polymerase chain reaction (RT-PCR), compared to an rDNA-based PCR assay.</p><p><strong>Methodology: </strong>Microbiological root canal samples were obtained from 32 teeth with primary endodontic infections before (S1) and after chemomechanical preparation (S2), and after removal of intracanal medication (S3). RNA and DNA were extracted, and complementary DNA (cDNA) was synthesized from RNA using RT reaction. cDNA and genomic DNA were subjected to PCR with primers complementary to the 16S rRNA sequences of Streptococcus spp. McNemar's test was used to compare the detection rate of both assays (P<0.05).</p><p><strong>Results: </strong>Streptococci were detected in 28.12% (9/32) and 37.5% (12/32) of S1 samples using rRNA- and rDNA-based PCR assays, respectively. In contrast, they were detected in only 6.25% (2/32) of S2 samples using rRNA-based RT-PCR, compared to 15.62% (5/32) using rDNA-based PCR. Finally, in S3 samples, streptococci were not detected by rRNA, whereas rDNA-based PCR still detected the bacteria in 12.5% (4/32) of the samples. The total number of PCR-positive reactions in the rDNA-based PCR was higher than in the rRNA-based assay (P<0.05).</p><p><strong>Conclusions: </strong>The rRNA-based RT-PCR showed a lower detection rate of streptococci when compared to the rDNA-based PCR, suggesting that the latter may have detected dead cells of streptococci in root canal samples.</p>","PeriodicalId":321675,"journal":{"name":"Journal of applied oral science : revista FOB","volume":" ","pages":"e20180256"},"PeriodicalIF":2.7,"publicationDate":"2019-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/1678-7757-2018-0256","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40452347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of the composition and shades of ceramics on light transmission and degree of conversion of dual-cured resin cements.","authors":"Luana Menezes de Mendonça,&nbsp;Ilana Santos Ramalho,&nbsp;Livia Aguiar Santos Nogueira Lima,&nbsp;Laís Alcântara Pires,&nbsp;Thiago Amadei Pegoraro,&nbsp;Luiz Fernando Pegoraro","doi":"10.1590/1678-7757-2018-0351","DOIUrl":"https://doi.org/10.1590/1678-7757-2018-0351","url":null,"abstract":"<p><strong>Objective: </strong>Since the transmittance of ceramics can influence the degree of conversion (DC) of resin cements, ceramics composition and shade should be considered in the selection of resin cement. This in vitro study aimed to evaluate the effect of the transmittance of different composition, opacities and shades of ceramics on the degree of conversion of two dual-cured resin cements.</p><p><strong>Methodology: </strong>Sixty discs were prepared from low translucency (LT) and medium opacity (MO) lithium disilicate ceramic, and zirconia ceramic (Z). Each group was subdivided into 5 subgroups (n=4) in shades A2, A3.5, B2, C2 and D3. The transmittance measurement was performed in a spectrophotometer. The Variolink II and Rely X U200 resin cements were photoactivated by LED (1400 mW/cm2) for 40 s through the ceramic discs and without the discs (control group). The DC was measured with infrared FTIR spectroscopy, immediately after light activation. Data were analyzed with Kruskall-Wallis and one-way ANOVA, following post-hoc comparisons by Tukey test and Pearson's correlation test (P<0.05).</p><p><strong>Results: </strong>LT ceramic exhibited higher transmittance values compared to MO and Z ceramics. LTA2 and LTB2 showed statistically higher transmittance values compared to MOA2, MOA3.5 and ZA3.5. For Variolink II, the ceramic interposition did not influence the DC, since there were no statistical differences between groups with ceramic interposition and the control group. For Rely X U200 cement, the interposition of some ceramics types/shades (LTA3.5, MOA2, MOA3.5 and ZA3.5) significantly decreased the DC values compared to control group. A positive correlation was found between the ceramic transmittance and DC values of both tested cements. Conclusions. The transmittance and DC values of the cements were influenced by composition and shades of the ceramics. The higher the transmittance of ceramics, the higher the DC values for both cements.</p>","PeriodicalId":321675,"journal":{"name":"Journal of applied oral science : revista FOB","volume":" ","pages":"e20180351"},"PeriodicalIF":2.7,"publicationDate":"2019-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/1678-7757-2018-0351","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40452354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of visfatin levels in gingival crevicular fluid as a potential biomarker in the relationship between obesity and periodontal disease.","authors":"Deniz Çetiner,&nbsp;Ahu Uraz,&nbsp;Seniha Öztoprak,&nbsp;Gülçin Akça","doi":"10.1590/1678-7757-2018-0365","DOIUrl":"https://doi.org/10.1590/1678-7757-2018-0365","url":null,"abstract":"<p><strong>Objectives: </strong>Visfatin is an adipokine that plays an important role in immune functions as a growth factor, enzyme, and pro-inflammatory mediator. We aimed to determine the levels of visfatin, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in gingival crevicular fluid (GCF) in both obese/non-obese patients, with/without generalized chronic periodontitis (GCP).</p><p><strong>Methodology: </strong>Patients were categorized as obese (O) (n=31) or non-obese (nO) (n=19). Groups were divided into four subgroups according to periodontal conditions: (1) periodontally healthy without obesity (nO-Ctrl); (2) GCP without obesity (nO-CP); (3) periodontally healthy with obesity (O-Ctrl); and (4) GCP with obesity (O-CP). Demographic variables, anthropometric and laboratory data were recorded. Periodontal parameters were measured at baseline and 3rd months after either non-surgical periodontal treatment or calorie -restricted diet therapy. At the same time, GCF samples were taken from patients to analyze TNF-alpha, IL-6,and visfatin levels.</p><p><strong>Results: </strong>Periodontal parameters were significantly higher in the O group than in the nO group (P<0.05). IL-6 levels were higher in the O group than in the nO group (P<0.001). The visfatin levels of the obese patients were reduceddecreased following the treatments (P<0.05). Cholesterol levels were higher in the O group than in the nO groups (P<0.05). IL-6 levels were higher in O-CP and O-Ctrl groups than in the nO-Ctrl group (P<0.05). Compared to the other groups, visfatin levels were significantly higher in the O-CP group but decreased following treatment (P<0.05).</p><p><strong>Conclusions: </strong>Our findings suggest that visfatin and IL-6 levels in GCF are associated with the pathogenesis of obesity and periodontitis. Within the limits of this study, we considered that there might be an association between the lipid profile and periodontitis on systemically healthy individuals.</p>","PeriodicalId":321675,"journal":{"name":"Journal of applied oral science : revista FOB","volume":" ","pages":"e20180365"},"PeriodicalIF":2.7,"publicationDate":"2019-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/1678-7757-2018-0365","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40452027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of bovine milk fermented with Lactobacillus rhamnosus L8020 on periodontal disease in individuals with intellectual disability: a randomized clinical trial.","authors":"Oda Yuki,&nbsp;Chiaki Furutani,&nbsp;Yuika Mizota,&nbsp;Atsuko Wakita,&nbsp;Sumiyo Mimura,&nbsp;Takuya Kihara,&nbsp;Masaru Ohara,&nbsp;Yoshiyuki Okada,&nbsp;Mitsugi Okada,&nbsp;Hiroki Nikawa","doi":"10.1590/1678-7757-2018-0564","DOIUrl":"https://doi.org/10.1590/1678-7757-2018-0564","url":null,"abstract":"<p><strong>Objective: </strong>Studies on the oral health of individuals with intellectual disability (ID) have identified problems that include a high prevalence of periodontal disease. The use of probiotics to treat periodontal disease has been the focus of considerable research, and bovine milk fermented with Lactobacillus rhamnosus L8020 (L8020 yogurt) has been shown to reduce the oral prevalence of four periodontal pathogens. The aim of this randomized, double-blind, placebo-controlled trial was to compare the effects of L8020 yogurt (test group) with those of placebo yogurt (placebo group) on the papillary-marginal-attached (PMA) index, gingival index (GI), and probing depth (PD) in 23 individuals with ID.</p><p><strong>Methodology: </strong>All patients were required to consume the allocated yogurt after breakfast for 90 days. PMA index and GI scores as well as PDs were assessed before the start of yogurt consumption (baseline), after 45 and 90 days of consumption, and 30 days after the cessation of consumption. Student's t-test, Mann-Whitney U test or Fisher's exact test was used for inter-group comparisons, and the mixed effect model of repeated measurements was used for data analysis.</p><p><strong>Results: </strong>The decrease in PMA index score was significantly greater in the test group than in the placebo group (p<0.001). The GI score also decreased during the study, with a tendency for greater decrease in the test group. Furthermore, decreases in PD between baseline, 45 and 90 days tended to be greater in the test group than in the placebo group.</p><p><strong>Conclusion: </strong>These results suggest that regular consumption of bovine milk fermented with L. rhamnosus L8020 can lower the risk of periodontal disease in individuals with ID.</p>","PeriodicalId":321675,"journal":{"name":"Journal of applied oral science : revista FOB","volume":" ","pages":"e20180564"},"PeriodicalIF":2.7,"publicationDate":"2019-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/1678-7757-2018-0564","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40541476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}