Journal of Veterinary Health Science最新文献

{"title":"Pedagogical intervention to improve didactics through science and innovation","authors":"A. J. Sierra, J. M. Brown, D. Maldonado, H. Sainz, Juan Francisco Viera González","doi":"10.21203/RS.3.RS-382693/V1","DOIUrl":"https://doi.org/10.21203/RS.3.RS-382693/V1","url":null,"abstract":"\u0000 The main objective of the article is to analyze the pedagogical intervention to improve didactics through science and innovation in the teaching-learning process of Sport Sciences and Physical Activity. The research presented is qualitative, the study is descriptive, based on a non-experimental design. The participating subjects were selected intentionally, confirmed by the 31 eighth semester students of the Bachelor's Degree in Physical Culture and Sports of the University of Sonora, 16 of them are related to the female gender (51.62%), the other students are male 15 (48.38%). Of the eight teachers, five are female (62.5%), while three are male (37.5%). The methods applied were observation, questionnaire, review of documents, inclusion criteria and statistical analysis verifying the levels of reliability and validity of the data, which gave us a confidence interval of (90%), with a significance value of p=0.1. The SPSS program version 23 to determine the statistics presented in the materials and methods to deepen the importance of the art of teaching and integrating science and innovation as part of the structural categories that make up the didactics as: content, objectives, methods, means and evaluation for the welfare of the quality of education.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121101765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin Induces MCF-7 Cells Pyroptosis Via Autophagy/CTSB/NLRP3/Caspase-1/GSDMD Signaling Pathway In Vitro And Vivo","authors":"Haonan Duan, Liping Jiang, Xiance Sun, Xiaofang Liu, Guang Yang, Xian-ce Sun, T-T Cheng, Yuchen Ji, Fan Zhang, Yue Du, Shu Ou, R. Ma, Xiu-ru Guan, Nan Teng, Xiaofeng Li","doi":"10.21203/RS.3.RS-263328/V1","DOIUrl":"https://doi.org/10.21203/RS.3.RS-263328/V1","url":null,"abstract":"\u0000 Background\u0000\u0000Curcumin, as a lipid-lowering drug, has been reported to be effective in the treatment of breast cancer. However, the underlying molecular mechanisms have not been completely investigated.\u0000Methods\u0000\u0000MTT assay was used to determine the effect of curcumin on survival rate of MCF-7 cells. The effects of curcumin on tumor growth were observed in animal models of breast cancer. The positive reactions of Caspase-1, IL-1β and IL-18 were detected by immunohistochemistry. LC3, p62, CTSB, ASC, Pro-Caspase-1, GSDMD, NLRP3, Caspase-1, GSDMD-N, IL-1β and IL-18 were determined by Western blot in vitro and vivo. The release of extracellular IL-1β and IL-18 was determined by ELISA. LDH release was measured. The expression level of CTSB in cytoplasm were determined by immunofluorescence assay. Cell proliferation, cell migration and tube formation assays were used to determine the abilities of cells. In this study, NLRP3 inflammasome inhibitor MCC950, cathepsin B inhibitor CA-074 ME and autophagy inhibitor 3-MA were used to act on cells to investigate the role of NLRP3 inflammasome, cathepsin B and autophagy in curcumin-induced pyroptosis of MCF-7 breast cancer cells.\u0000Results\u0000\u0000In mouse model of breast cancer, we observed that curcumin treatment significantly induced cell autophagy and pyroptosis. In human breast cancer MCF-7 cells, we found that curcumin induced pyroptotic cell death was dependent on the activation of NLRP3/Caspase-1/GSDMD signaling pathway, which was CTSB-dependent. In addition, curcumin-induced cell autophagy caused lysosomal rupture and CTSB release. Furthermore, NLRP3 inhibitor (MCC950) significantly suppressed curcumin-induced pyroptosis, as well as CTSB inhibitor (CA074 Me) and autophagy inhibitor (3-MA). Besides, we also found that curcumin suppressed cell proliferation, cell migration and tube formation, which could be reversed by inhibitors.\u0000Conclusions\u0000\u0000In summary, our results demonstrated that curcumin induced MCF-7 cell pyroptosis by the activation of autophagy/CTSB/NLRP3/Caspase-1/GSDMD signaling pathway. These findings offer novel insights into the potential molecular mechanisms of curcumin in treatment of breast cancer.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133680096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of natural staining on Wistar rat EMI region using different metal salts","authors":"","doi":"10.33140/jvhs.02.01.04","DOIUrl":"https://doi.org/10.33140/jvhs.02.01.04","url":null,"abstract":"Background: The natural staining of cells has recently become a growing research topic. Roselle (Hibiscus sabdariffa) chemical structure has two main components that contain more chromophore groups. Objectives: The aim of this study is to dye cells and elements in two important parts of the Wistar rat uterus with roselle extract containing dense flavonoids and metal salts. Methods: In this study, dried and clean Hibiscus sabdariffa obtained from the local market was used for histological staining of uterine layers (EMI region) in Wistar rat tissue. Different cell structures and their different pathological evaluations are made in the EMI region. In order to more reach different cells, solutions of one percent anhydrous SnCl2 (tin chloride), 1% FeSO4.5H2O (ferrous sulfate), and 1% KAlSO4.12H2O (alum) metal salts in distilled water were used in this study. Results: In staining with roselle extracts containing alum and iron sulfate, tissue red-colored endometrial cell staining, and bluepurple stained inflammatory cells were observed in staining with roselle extract containing 1% SnCl2. Conclusions: Cytoplasmic and nuclear staining intensity, bluing, and clarity were better when SnCl2 was used as the bluing or bruising agent. A purple chelates and coordinate covalent bonding between Flavonoids in the roselle structure and the protein structures in the cell was formed by tin metal in SnCl2 solution. In fact, that there are blue-purple-stained regions in different shades in the cell structure suggests that flavonoids stain different uterine cells thanks to SnCl2. This situation may help to evaluate different and complex pathological findings in the uterus.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124552552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Practical and Modern Composting Techniques in Kuwait","authors":"","doi":"10.33140/jvhs.02.01.01","DOIUrl":"https://doi.org/10.33140/jvhs.02.01.01","url":null,"abstract":"Kuwait imports more than 2 million sheep per year to feed its population. Moreover, Kuwait City produces 3.5 million kg of compostable municipal waste each day. Lastly, Desert Storm and Desert Shield has produced 120 oil lakes that need remediation. These three major environmental factors could be resolved by a simple solution, composting. This preliminary study focused on composting carcasses from the imported sheep and associated losses due to transportation. The unique climate conditions in Kuwait presented different challenges to composting. This study is the first step in addressing these environmental concerns. This research was divided into 2 sections: 1) classic cement slab composting of dead sheep with microbiology assessment, and 2) Tri-Form Poly Ecodrum® composting of sheep with microbiology assessment. The classic method using the cement slab was modified by eliminating the turning step used to keep the process aerobic. Instead, air was introduced directly into the piles of compost. This modification did not shorten the time needed for composting but reduced the manual labor needed for the turning of piles. The Ecodrum® accelerated the composting process by at least five-fold; the four months processing by the traditional cement slab method was reduced to only 20 to 25 days using the Ecodrum®. This is the first report that investigates and details the comparison between classical and modern composting techniques to show their equivalency. The color and texture of the Ecodrum® and the cement slab products were similar. This report details the bacterial and fungal tests for the different compost products..","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121673651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of adverse drug reaction (ADR) in ruminants in various parts of Maharashtra","authors":"","doi":"10.33140/jvhs.02.01.02","DOIUrl":"https://doi.org/10.33140/jvhs.02.01.02","url":null,"abstract":"For better understanding the drug safety program, reporting of the adverse drug reaction and adverse drug events are quite well organized in the human pharmacovigillance in India. Many aspects of the ADR/ADE are even unknown to the users of Veterinary medicinal products. Smallest to smallest as well as biggest ADR is to be reported to respective branches of government agency. Sudden exposure to complicated guidelines may leads to confusion and work intolerance in personals that are handling / using the various veterinary medicinal products for the treatment and betterment of animal health. A step by step understanding is a need of time for the field of veterinary pharmacovigillance (PV), as most of the users of VMP are staying in rural parts of India and may not be that much educated. Hence through interviews from 500 Field Veterinarians, data of last 5 years was analyzed and presented for this paper. Based on the severity on the health of the animals, ADR/ADEs were classified as Minimal, moderate, severe and accidental. Most of the time severe ADR/ADEs leads to death in ruminants.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117043517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Type IV Secretion System in Brucella Virulence: A Review","authors":"","doi":"10.33140/jvhs.02.01.07","DOIUrl":"https://doi.org/10.33140/jvhs.02.01.07","url":null,"abstract":"Brucellosis is a bacterial disease of domestic and wild animals caused by the genus Brucella which has great public health importance globally. In general, Brucella species do not express toxins or virulence factors that cause direct damage to the host. Instead, this pathogen’s strategy is to persist long enough in the infected host until transmission can occur, which in the natural hosts is usually through abortion, sexual contact, or shedding of bacteria in milk. One important Brucellavirulence factor for intracellular survival and persistence in the host is the type IV secretion system (T4SS).The type IV secretion system (T4SS) is one of numerous secretion systems used by microbes to transfer macromolecules through the cell membrane, such as proteins and DNA. It’s the most versatile secretion system, transporting monomeric proteins, multi-subunit protein poisons, and nucleoprotein complexes, and it’s found in Gram-positive and Gram-negative bacteria, as well as some Archaea. The type IV secretion system is a major Brucella virulence factor for intracellular survival and host persistence. This review will go through the present state of knowledge on the Brucella type IV secretion system, including its architecture and regulation, as well as the newly discovered effector substrates that this system delivers into host cells.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130616026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benefits of drinking dissolved oxygenated water for older dogs","authors":"","doi":"10.33140/jvhs.02.01.05","DOIUrl":"https://doi.org/10.33140/jvhs.02.01.05","url":null,"abstract":"Objective: Oxygen is an essential factor in the body. As dissolved oxygen is primarily absorbed through the gastrointestinal tract, this study aimed to investigate whether oxygenated water (commercial: water 0) reduced serum lactate levels in old dogs under conditions of rest and exercise. Materials and Methods: Beagle dogs (n = 8), unneutered males (n = 4), and unspayed females (n = 4), aged 8.1 ± 1.3 years and weighing 12.2 ± 1.2 kg, considered healthy by physical examination and blood tests, were included in the study. Results: Oxygenated water (5 ml; Water 0, Inc.) was fed per 1 kg of body weight. The blood lactate level was significantly decreased in the group fed with O2 supplementation in the usual environment without exercise than in the control group (1.97 ± 0.19 vs. 2.63 ± 0.3 mmol/L). In addition, the blood lactate level after 30 min of walking was markedly decreased in the oxygen supplemented water-fed group than in the control group (2.46 ± 0.38 in the control group vs. 1.66 ± 0.21 mmol/L in the oxygen supplemented water-fed group). Conclusion: In this study, the efficacy and benefits of dissolved oxygen feeding were demonstrated in old dogs both at rest and after exercise.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124768721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Injury causing Road Traffic Accident at Debre Markos Town","authors":"Wubetie Habtamu Tilaye","doi":"10.21203/rs.3.rs-101705/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-101705/v1","url":null,"abstract":"\u0000 Objective: A Road traffic accident (RTA) is when in a road Vehicle collides with another Vehicle, pedestrian, animal or geographical or architectural obstacle. The RTAs can result in human injury, property damage and death. RTA result in the deaths of 1.2 million people worldwide each year and injuries about 4 times this number. The objective of this study is to identify the main causing-factors that contribute to road traffic accidents involving human injuries. Literature suggested factors considered for analysis are: Driver's Age, Driver’s Education status, Driver's experience, Vehicle type, Driver Vehicle Ownership, Vehicle year of service, Road type, Road division, Road condition, Problem with car, Weather condition, and accident time (day or night). Results: Among the candidate variables, Pearson Chi-Square method identified weather condition, driver’s experience, Vehicle year of service; Road division, Driver Vehicle Ownership, and accident time (day or night ) as significantly associated variables. Furthermore, percentage is used to describe the magnitude of associated variable. The result from Poisson regression analyses revealed that low driver experience, high Vehicle year of service (old cars), and Road division (one way road) are the significant contributing factors for increment of human injuries accidents.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116856400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug Esfenvalerate: Diagnosis, Treatment and Prevention","authors":"","doi":"10.33140/jvhs.01.01.02","DOIUrl":"https://doi.org/10.33140/jvhs.01.01.02","url":null,"abstract":"The article presents identified causes, clinical symptoms and pathological anatomy of poisons, also developed by effective measures diagnosis, prevention and treatment of poison rabbits with pyretroid esphenvaleriat.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117240762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foot and Mouth Disease Virus Infection Seroprevalence Study in Dairy Cattle Reared by Smallholder Farmers in Welmera District, Central, Oromiya Ethiopia","authors":"","doi":"10.33140/jvhs.01.01.03","DOIUrl":"https://doi.org/10.33140/jvhs.01.01.03","url":null,"abstract":"Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals and one of the endemic diseases in Ethiopia. A study was conducted to determine foot and mouth disease virus seroprevalence and potential risk factors in welmera district, central Oromiya, Ethiopia. Purposive sampling was performed in the respective district and kebeles where there was recent history of outbreaks. A total of 126 sera samples were collected from randomly selected cattle and tested using ELISA for antibodies against nonstructural proteins of foot and mouth disease virus. The seroprevalence of foot and mouth disease virus infection in Welmera district was 49.2%. The result indicated that seropositivity of FMD infection FMD was higher in females (54%) than in males (46.1%) and this variation was statistically significant (χ2= 12.93, p = 0.001). The associations of seropositivity of FMD in relation to body condition score and management system were statistically significant (χ2= 5.34, p = 0.023, χ2= 6.7, p = 0.003) respectively. Age related seropositivity was not statistically significant (P>5%). Cross bred cattle were 2.17 (OR = 2.17, 95% CI: 2.10 – 5.74) times more likely to be infected by FMD than local breeds. This showed that FMD infection is widely prevalent in the district. Therefore, foot and mouth disease control will depend on identifying virus serotypes circulating in the area and regular vaccination program.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129113396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}