Evaluation of natural staining on Wistar rat EMI region using different metal salts

Background: The natural staining of cells has recently become a growing research topic. Roselle (Hibiscus sabdariffa) chemical structure has two main components that contain more chromophore groups. Objectives: The aim of this study is to dye cells and elements in two important parts of the Wistar rat uterus with roselle extract containing dense flavonoids and metal salts. Methods: In this study, dried and clean Hibiscus sabdariffa obtained from the local market was used for histological staining of uterine layers (EMI region) in Wistar rat tissue. Different cell structures and their different pathological evaluations are made in the EMI region. In order to more reach different cells, solutions of one percent anhydrous SnCl2 (tin chloride), 1% FeSO4.5H2O (ferrous sulfate), and 1% KAlSO4.12H2O (alum) metal salts in distilled water were used in this study. Results: In staining with roselle extracts containing alum and iron sulfate, tissue red-colored endometrial cell staining, and bluepurple stained inflammatory cells were observed in staining with roselle extract containing 1% SnCl2. Conclusions: Cytoplasmic and nuclear staining intensity, bluing, and clarity were better when SnCl2 was used as the bluing or bruising agent. A purple chelates and coordinate covalent bonding between Flavonoids in the roselle structure and the protein structures in the cell was formed by tin metal in SnCl2 solution. In fact, that there are blue-purple-stained regions in different shades in the cell structure suggests that flavonoids stain different uterine cells thanks to SnCl2. This situation may help to evaluate different and complex pathological findings in the uterus.