Sains Medika最新文献

Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke 四种抗氧化剂组合对暴露于香烟烟雾大鼠睾丸和间质细胞数量、精子质量和Caspase-3表达的影响

Sains Medika Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.2465

Conita Yuniarifa, Joko Wahyu Wibowo, T. Nasihun

{"title":"Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke","authors":"Conita Yuniarifa, Joko Wahyu Wibowo, T. Nasihun","doi":"10.30659/SAINSMED.V11I2.2465","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I2.2465","url":null,"abstract":"Introduction : The administration of antioxidant combination has been shown to be more effective than that of individual antioxidant in male infertility. Objective : to determine the effect of different combination of antioxidants (vitamin/vit C, vit E, glutathione and zinc) on the number of Sertoli and Leydig cells, sperm quality and texpression of caspase-3 in rats exposed to cigarette smoke. Methods : This was an experimental study using a post test only control group design. Thirty six rats were randomly divided into 6 groups to receive one of the following combinations of antioxidants: vit C and E, glutathione, zinc (T4-G); vit C and E, glutathione (T3-G); vit C and vit E (T2-G); Glutathione, zinc (T1-G); control positive (CP-G) cigarette smoke exposed only, normal (CN-G). The dose of vit C, vit E, glutathion and zinc was 9 mg/day, 1.8 IU/day, 1.8 mg/day, 0.2 mg/day respectively. All groups were exposed to cigarette smoke (3 cigarettes/day for 21 days), except for the CN-G group. On day 22, sperm samples were taken. Testicular tissue was taken for measurements of sperm quality, sertoli and leydig cell.numbers. Results : There was a significant difference in mean number of Leydig cells, sperm motility, sperm count and caspase-3 between groups (p<0.05). Post hoc LSD showed that the administration of combination of four antioxidant agent increasing the number of Sertoli cells and sperm morphology. The results also showed that group treated with 4 antioxidants combination had the highest number of Leydig cells, sperm motility, sperm counts and the lowest expression of caspase-3. Conclusion :The combination of vitC, vit E, glutathione and zinc affects the number of Sertoli and Leydig cells, sperm quality, expression of caspase-3 in rats exposed to cigarette smoke .","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46357661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Antlion (Myrmeleon sp.) Extract Towards Histopathology Image of Pancreas in Diabetic Mice 蚁提取物对糖尿病小鼠胰腺组织病理学图像的影响

Sains Medika Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.7656

Muhammad Naufal Widyatmaka, Akhmad Ismail

{"title":"The Effect of Antlion (Myrmeleon sp.) Extract Towards Histopathology Image of Pancreas in Diabetic Mice","authors":"Muhammad Naufal Widyatmaka, Akhmad Ismail","doi":"10.30659/SAINSMED.V11I2.7656","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I2.7656","url":null,"abstract":"Introduction: Some studies indicated that antlions (Myrmeleon sp.) is traditionally used by many people in Indonesia as a diabetes treatmenter. However. reseach has not been done to find the effect of antlion extract in pancreas, an important organ in diabetes mellitus pathophysiology. Objective: To prove the effect of antlion extract towards histopathological feature of pancreas in diabetic mice.Methods: In the post test only control group design, 25 Swiss mice were divided into five groups. Normal group (Nor-G), Negative control group (Neg-G), and the other 3 groups were intraperitoneally injected with Streptozotocine 200 mg/kg to induce diabetic state. In Neg-G had no antlion treatment, whereas in At-14, At-28, and At-56 were treated with antlion extract at the dose of 14, 28, and 56 mg/kg BW respectively for 24 days. Pancreas then collected and prepared to be examined under microscope with 400x magnification. The score of pancreatic islet injury was determined by assessing structural damage and inflammatory cells infiltration. Kruskal-Wallis and Mann-Whitney were adopted as statistical analysis.Results: Mann-Whitney analysis indicated that the score of pancreatic islets injury in At-56 was significant lower compared to that of Neg-G, p<0.05. The score of pancreatic islets injury in At-56 also significant lower compared to that of At-14 and At-28, p<0.05. In contrary, when compared to that of Nor-G, the pancreatic islets injury in At-56 was not signicantly lower, p>0.05.Conclusion: Antlion extract treatment at the dose of 56 mg/kg BW was capable of reducing pancreatic islet injury in diabetic mice induced by streptozotocine.","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42921611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4 CMCE蜂胶提取物提高CCl4暴露大鼠肝细胞Caspase 3表达及IL-6水平

Sains Medika Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.5190

Chalid Firdaus, Chodidjah Chodidjah, T. Nasihun

{"title":"CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4","authors":"Chalid Firdaus, Chodidjah Chodidjah, T. Nasihun","doi":"10.30659/SAINSMED.V11I2.5190","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I2.5190","url":null,"abstract":"Introduction : Liver plays an important role in the metabolic processes possibly exposed to the toxic materials. One of those hepatotoxic substances is carbon tetrachloride. The propolis extract contains the balsamic active substances, CAPE compounds and flavonoids. Flavonoids can prevent from the apoptosis and reduce the inflammation. Objective : To validate propolis extract’s (CMCE method) ability in improving caspase 3 expressions of hepatocytes and IL-6 levels in Wistar male rats induced with carbon tetrachloride. Methods : An experimental study with a post-test only control group design applied to the study. The research subjects were 28 male Wistar rats, divided into four groups. The negative control group (CN-G) was only injected with CCl4, while the experimental groups were administered with the CMCE propolis at the dosages of 3.6 mg/200g (CM3-G), 7.2 mg/200g (CM7-G), and 14.4 mg/200g (CM14-G). The CMCE propolis extract was administered for 14 days and on day 14, the CCl4 was then given. The caspase 3 expression of hepatocytes was measured using HE liver cell preparations, while the IL-6 levels were measured using ELISA method. Results : The results of Mann-Whitney U statistical analysis showed that the hepatocytes’ caspase 3 expressions in CM14-G (2.77 ± 0.531), CM7-G (3.14 ± 0.378), and CM3-G (4.22 ± 0.690) were lower than those in CN-G (5.43 ± 0.535), p<0.05. Meanwhile, the Post Hoc LSD analysis Results showed that IL-6 levels in CM14-G (55.032 ± 9.336), CM7-G (78.362 ± 8.313), and CM3-G (114.975 ± 10.359) were lower those in CN-G (180.301 ± 5.428), p<0.05. Conclusion : the administration of propolis extract (CMCE method) improve the caspase 3 expressions of hepatocytes and Il-6 levels in Wistar male rats induced with carbon tetrachloride.","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42852663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purslane (Portulaca oleracea) Ethanol Extract decreases hs-CRP levels and Total Score of Renal Tubular Degeneration in Rat Model of Gentamicine-Induced Renal Damage 马齿苋乙醇提取物降低庆大霉素致肾损伤大鼠hs-CRP水平及肾小管变性总评分

Sains Medika Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.4092

Ragil Saptaningtyas, Shofa Chasani, T. Nasihun

{"title":"Purslane (Portulaca oleracea) Ethanol Extract decreases hs-CRP levels and Total Score of Renal Tubular Degeneration in Rat Model of Gentamicine-Induced Renal Damage","authors":"Ragil Saptaningtyas, Shofa Chasani, T. Nasihun","doi":"10.30659/SAINSMED.V11I2.4092","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I2.4092","url":null,"abstract":"Introduction: Injection of intraperitoneal gentamicin at a dose of 60 mg/kg BW intra-peritoneal in rats for 7 days has been shown to trigger renal tubular degeneration and increase in high sensitivity C-reactive protein (hs-CRP) level. The use of purslane (Portulaca oleracea) to reduce hs-CRP levels and total score of renal tubular degeneration has not been reported.Objective: to determine the effect of purslane ethanol extract on hs-CRP levels and renal tubular degeneration score in gentamicin induced-renal damage in rats.Methods: This was a study using posttest only control group design. Twentyfive male Wistar rats aged 8-12 weeks, weighed 150-200 grams were allocated into 5 groups: normal (Nor-G), without treatment; negative (Neg-G), gentamicin (60 mg/kg BW), intraperitoneally; P-200; P-300 ; P-400 (gentamicin plus purslane extract at a dose of 200, 300, and 400 respectively) for 7 days. The hs-CRP was evaluated using the ELISA method. Total score of renal tubular degeneration was evaluated using modified score of Sarjadi.Results: there was a significant difference in mean hsCRP level and total score of renal tubular degeneration between groups (p <0.05). Posthoc analysis showed that hsCRP level and total score group of P-200, P-300 and P-400 were significantly lower than those of Neg-G, p <0.05. Meanwhile, the levels of hsCRP and total score of renal tubular degeneration in P-200, P-300, P-400, and Nor-G groups were not significantly different (p> 0.05).Conclusion: the administration of purslane ethanol extract at doses of 200, 300, and 400 mg/kg BW for 7 days improve hs-CRP level and total score of tubular degeneration similar to normal. ","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49190338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Senam Sehat Anak Indonesia Improve Peak Expiratory Flow Rate in Children with Obesity 改善肥胖儿童的呼气流量峰值

Sains Medika Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.7598

F. Ramadhania, Rahmi Isma Asmara Putri, Erna Setiawati, Yetty M Nency

{"title":"Senam Sehat Anak Indonesia Improve Peak Expiratory Flow Rate in Children with Obesity","authors":"F. Ramadhania, Rahmi Isma Asmara Putri, Erna Setiawati, Yetty M Nency","doi":"10.30659/SAINSMED.V11I2.7598","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I2.7598","url":null,"abstract":"Introduction: Excessive fat accumulation in the obese child causes complex problems in the respiratory system. Respiratory system function can be improved by exercise. One exercise model that is suitable for children is Senam Sehat Anak Indonesia (SSAI). The quality of the respiratory function system is measured using Peak Expiratory Flow Rate (PEFR).Objective: to validate the PEFR’s differences in obese children before and after performing SSAI.Methods: This research using one group pre-posttest experimental design with total sample of 20 obese children using purposive sampling method. The research instrument uses weight scale, height scale, and peak flow meter. SSAI was given to all subjects twice a week for six weeks. Paired T Test statistical analysis was used to examine the PEFR’s differences before and after performing SSAI, considered significant if p<0.05.Results: The PEFR’s mean of all subjects before performing SSAI was 258.25 ± 39.51 L∕minute, while PEFR’s mean after performing SSAI twice a week for 6 weeks was 282.50 ± 43.03 L∕minute, (p=0,000).Conclusion: The PEFR’s mean of obese children was increase significantly after performing SSAI twice a week for 6 weeks","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42723137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis 局部施用芦荟提取物可减少UVB光照后表皮上晒伤细胞的数量和半胱天冬酶-3的表达

Sains Medika Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.9094

Kusumawati Soetrisno, P. Subchan, Atina Hussana

{"title":"The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis","authors":"Kusumawati Soetrisno, P. Subchan, Atina Hussana","doi":"10.30659/SAINSMED.V11I2.9094","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I2.9094","url":null,"abstract":"Introduction : Ultraviolet B (UVB) radiation triggers the formation of free radicals that cause apoptosis and sunburn cells (SBC) formation. Aloe vera contains anti-inflammatory and antioxidant compounds that can potentially inhibit this process. Objective : to assess the effect of topical Aloe vera extract administration on the decrease of SBC number and Caspase-3 expression on the epidermis after UVB light exposure. Methods : In a post-test only group design study, a single dose of 3 doses of Erythema (DEM) was performed on two groups of 6 week old BALB/c female rats. Group A (control) did not receive any topical treatment, and group B (treatment) were smeared with 75% Aloe vera extract before irradiation. Each group was divided into 4 sub-groups based on post-exposure time of 6 hours (A1 and B1), 12 hours (A2 and B2), 24 hours (A3 and B3), and 48 hours (A4 and B4). The expression of caspase-3 was assessed by immunohistochemical staining while the SBC number was measured using a microscope. The expression of caspase-3 was analyzed using the Kruskal-Wallis and Mann-Whitney statistical tests, while the number of SBC was analyzed using the one-way ANOVA statistical test and the post-hoc LSD test. Results : The results of the Kruskal-Wallis analysis showed a significant difference in Caspase-3 expression between groups, p <0.05. The results of Mann-Whitney analysis showed a significant difference in Caspase-3 expression between the A1-A3 and B1-B3 subgroups (p <0.05). One-way ANOVA analysis showed a significant difference in the number of SBC between groups (p <0.05). Post-hoc LSD analysis showed significant differences in SBC counts between groups A and B across all subgroups, p <0.05. Conclusion : Topical application of Aloe Vera extract decreased Caspase-3 expression and the number of SBC in UVB light-exposed skin.","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49556604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparison between Insulin-like Growth Factor Binding Protein-1 Level and Bishop Score as Predictor of Successful Labor Induction in Full Term Pregnancy with Preeclampsia 胰岛素样生长因子结合蛋白-1水平与Bishop评分作为先兆子痫足月妊娠引产成功预测指标的比较

Sains Medika Pub Date : 2021-01-04 DOI: 10.30659/SAINSMED.V11I2.10919

Nurul Setiyorini, Ratna Cahyanti

{"title":"Comparison between Insulin-like Growth Factor Binding Protein-1 Level and Bishop Score as Predictor of Successful Labor Induction in Full Term Pregnancy with Preeclampsia","authors":"Nurul Setiyorini, Ratna Cahyanti","doi":"10.30659/SAINSMED.V11I2.10919","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I2.10919","url":null,"abstract":"Introduction: preeclampsia/eclampsia contributed to 30% of maternal mortality in RSUP Dr. Kariadi. Pre-induction bishop score assessment is a standard for cervical maturity estimation. Other predictors currently being developed, including insulin-like growth factor binding protein-1 (IGFBP-1). However, there is no cut-off point for IGFBP-1 examination in full term pregnancy, especially with preeclampsia and its complications. Objective: to find IGFBP-1 levels and Bishop Score differences in preeclampsia women with induction labor.Methods: this prospective observational cohort study was conducted in preeclampsia women with gestational age ≥37 weeks planned labor induction at Obstetrics and Gynecology Ward RSUP Dr. Kariadi Semarang and networking hospital during study period (n=66). History taking, physical examination, proteinuria, cervical mucus IGFBP-1, and bishop score calculation was performed. Statistical analysis was performed with paired-t test, followed by ROC for cut-off value of successful induction prediction, and logistic regression to determine confounding variables effect on IGFBP-1 levels.Results: The average Bishop score of 66 subjects was 2.5±1.81 and 2.6±1.8 for women who deliver ≤24 labor and in labor for ≤12 hours, respectively. Although the bishop score was higher in the successful labor group, there was no significant difference between groups. Mean IGFBP-1 value was 8.29±5.033 mcg/L with median value of 10.8 mcg/L. Successful induction had significant higher IGFBP-1 levels with area-under-curve (AUC) 0.76 and 8.145 cutoff value (p=0.002, RR=5.1).Conclusion: IGFBP-1 level with cutoff point 8.145 can be used as predictor of successful labor induction in term pregnancy with preeclampsia.","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47175901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Honey in Second Degree Burn Healing on Wistar Rats (Overview of Angiogenesis and the Number of Fibroblasts) 蜂蜜对Wistar大鼠二度烧伤愈合的影响(血管生成及成纤维细胞数量综述)

Sains Medika Pub Date : 2020-09-13 DOI: 10.30659/SAINSMED.V11I1.7614

Ayyasi Izaz Almaz, R. D. Purnawati, H. Istiadi, N. Susilaningsih

{"title":"The Effect of Honey in Second Degree Burn Healing on Wistar Rats (Overview of Angiogenesis and the Number of Fibroblasts)","authors":"Ayyasi Izaz Almaz, R. D. Purnawati, H. Istiadi, N. Susilaningsih","doi":"10.30659/SAINSMED.V11I1.7614","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I1.7614","url":null,"abstract":"Introduction: Burn therapy is generally done using a burn ointment. However, honey can also be used as an alternative treatment for burns. Honey contains active flavonoid as an antioxidant and hydrogen peroxide which can stimulate fibroblasts proliferation also angiogenesis on burn healing phase.Objective: to determine the effect of honey in second degree burn healing from angiogenesis and the number of fibroblasts on Wistar rats. Methods: This research used a posttest only controlled group design on 15 rats with random selection and divided into 3 groups: H-G was given honey, B-G was given Bioplacenton�, N-G without any treatment after burn was induced. Rats were given a second degree burn in a size of 2 cm x 2cm on the back area and were treated twice a day for 14 days. Burn healing was measured by calculating the observation results of microscopic VEGF expression, the number of neovascularization, and the number of fibroblasts. Hypothesis analysis used Annova post hoc LSD and Mann-Whitney.Results: The highest mean in VEGF expression and the number of neovascularization was obtained from group B-G followed by group H-G and group N-G. Significant differences in the number of neovascularization between group B-G with group N-G, p<0.05.Conclusion: Honey can provide a healing effect on the second degree of burns in Wistar rats in terms of the number of neovascularization as well as Bioplacenton�","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46713808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Influence of Continuous Multi Stage Countercurrent Extraction Process (CMCE) Propolis Extract Administration on Superoxide Dismutase Activity and Hepatocytes Fibrosis Degree (An Experimental Study on Male Wistar Rats Induced with Carbon Tetrachloride (CCl4)) 连续多级逆流提取蜂胶提取物对超氧化物歧化酶活性和肝细胞纤维化程度的影响（四氯化碳诱导雄性Wistar大鼠的实验研究）

Sains Medika Pub Date : 2020-08-03 DOI: 10.30659/SAINSMED.V11I1.5000

Elsa Wani, T. Nasihun, Atina Hussaana

{"title":"The Influence of Continuous Multi Stage Countercurrent Extraction Process (CMCE) Propolis Extract Administration on Superoxide Dismutase Activity and Hepatocytes Fibrosis Degree (An Experimental Study on Male Wistar Rats Induced with Carbon Tetrachloride (CCl4))","authors":"Elsa Wani, T. Nasihun, Atina Hussaana","doi":"10.30659/SAINSMED.V11I1.5000","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I1.5000","url":null,"abstract":"Introduction: Continuous Multi Stage Countercurrent Extraction Process (CMCE) propolis extract containing Caffeic Acid Phenethyl Ester (CAPE) may inhibit the formation of lipid peroxidation. However, it is still unclear whether or not CMCE propolis is able to increase SOD activity and reduce acute hepatocytes damages induced by CCl4. Objective: To evaluate the influence of CMCE propolis extract administration in increasing the SOD activity and reducing the hepatocytes fibrosis of male Wistar rats induced with CCl4. Method: an experimental research with a post-test only control group design. Twenty eight male Wistar rats were divided into four groups. Group C-N was only induced with CCl4, while group CM-3, CM-7, and CM-14 were induced with CCl4 and received CMCE propolis extract respectively with 3.6 mg/200 g BW, 7.2 mg/200 g BW, and 14.4 mg/200 g BW. CMCE propolis was administered for 14 days and then CCl4 was administered on the last day. SOD activity was measured using the colorimetric method, while the hepatocytes fibrosis degree was assessed using Image J Software method with NASH score modification. Results: Post Hoc LSD analysis indicated that SOD activity in group CM-3 (51.67 ± 4.20), CM-7 (70.21 ± 6.26), CM-14 (80.85 ± 4.59) was lower than that in group C-N (23.70 ± 5.12) with P <0.05. Meanwhile, the Mann-Whitney U test showed that the hepatocytes fibrosis degree in group CM-3 (0.85 ± 0.69), CM-7 (1.14 ± 0.37), and CM-14 (0.71 ± 0.48) was significantly lower than that in group C-N (2.28 ± 1.25) with p<0.05. Conclusion: The CMCE propolis extract administration was able to increase the SOD activity and reduce the hepatocytes fibrosis degree of male Wistar rats induced with CCl4","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44668341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Immunomodulatory Effect of Cinnamon (Cinnamomum Burmanii) Bark Extract On the C-Reactive Protein (CRP) Level, Leukocyte Count and Leukocyte Type Count of Wistar Rats Exposed to Staphylococcus Aureus 肉桂(Cinnamomum Burmanii)树皮提取物对金黄色葡萄球菌暴露Wistar大鼠c反应蛋白(CRP)水平、白细胞计数和白细胞类型计数的免疫调节作用

Sains Medika Pub Date : 2020-07-05 DOI: 10.30659/SAINSMED.V11I1.4907

Astika Widy Utomo, D. Retnoningrum, A. Gumay

{"title":"The Immunomodulatory Effect of Cinnamon (Cinnamomum Burmanii) Bark Extract On the C-Reactive Protein (CRP) Level, Leukocyte Count and Leukocyte Type Count of Wistar Rats Exposed to Staphylococcus Aureus","authors":"Astika Widy Utomo, D. Retnoningrum, A. Gumay","doi":"10.30659/SAINSMED.V11I1.4907","DOIUrl":"https://doi.org/10.30659/SAINSMED.V11I1.4907","url":null,"abstract":"Introduction: Bacterial infection induces inflammation in human body. This process produceshumoral and cellular immune responses. Cinnamomum burmanii grows very vast in Indonesia and contains cinnamaldehyde known to have an anti-inflammatory effect. Objective : To prove the effect of C. burmanii bark extract on CRP level, leukocyte count and differential blood count. Methods: Aposttest-only controlled group design with 25 Wistar Rats divided into 5 groups was employed. The CN-G group was giventhe standard feed, the CP-G group was given the standard feed and levamisole 2.5 mg/KgBW, while the CBE-100, CBE-200, and CBE-400 groups were respectively given the standard feed and cinnamon bark extract 100 mg/kgBW, 200 mg/KgBW and 400 mg/KgBW. The treatmentswereconducted for 7 consecutive days.On day 8, all rats were injected with the suspense of S. aureus intraperitoneally. The blood wasthen drawn on day 9, followed with CRP level measurement using the ELISA method. The total leukocyte count and differential blood count weremanually measured. Results: There is no significant difference in the value of CRP level (One Way ANOVA; p = 0.749) with the total counts of leukocytes(p=0.685), monocytes (p=0.769), and eosinophil(p=0.123) between groups. The neutrophils and lymphocytes of CBE-100 group aresignificantly differentfrom the other groups. Conclusion: C. burmanii extract has a potential benefit as immunomodulator.","PeriodicalId":31696,"journal":{"name":"Sains Medika","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42405794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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