The Effect of Antlion (Myrmeleon sp.) Extract Towards Histopathology Image of Pancreas in Diabetic Mice

Muhammad Naufal Widyatmaka, Akhmad Ismail
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Abstract

Introduction: Some studies indicated that antlions (Myrmeleon sp.) is traditionally used by many people in Indonesia as a diabetes treatmenter. However. reseach has not been done to find the effect of antlion extract in pancreas, an important organ in diabetes mellitus pathophysiology. Objective: To prove the effect of antlion extract towards histopathological feature of pancreas in diabetic mice.Methods: In the post test only control group design, 25 Swiss mice were divided into five groups. Normal group (Nor-G), Negative control group (Neg-G), and the other 3 groups were intraperitoneally injected with Streptozotocine 200 mg/kg to induce diabetic state. In Neg-G had no antlion treatment, whereas in At-14, At-28, and At-56 were treated with antlion extract at the dose of 14, 28, and 56 mg/kg BW respectively for 24 days. Pancreas then collected and prepared to be examined under microscope with 400x magnification. The score of pancreatic islet injury was determined by assessing structural damage and inflammatory cells infiltration. Kruskal-Wallis and Mann-Whitney were adopted as statistical analysis.Results: Mann-Whitney analysis indicated that the score of pancreatic islets injury in At-56 was significant lower compared to that of Neg-G, p<0.05. The score of pancreatic islets injury in At-56 also significant lower compared to that of At-14 and At-28, p<0.05. In contrary, when compared to that of Nor-G, the pancreatic islets injury in At-56 was not signicantly lower, p>0.05.Conclusion: Antlion extract treatment at the dose of 56 mg/kg BW was capable of reducing pancreatic islet injury in diabetic mice induced by streptozotocine.
蚁提取物对糖尿病小鼠胰腺组织病理学图像的影响
引言:一些研究表明,蚂蚁(Myrmeleon sp.)在印度尼西亚传统上被许多人用作糖尿病治疗药物。然而蚂蚁提取物对糖尿病病理生理学中的重要器官胰腺的作用尚未得到研究。目的:证实蚂蚁提取物对糖尿病小鼠胰腺组织病理学特征的影响。方法:在仅试验后的对照组设计中,25只瑞士小鼠被分为5组。正常组(Nor-G)、阴性对照组(Neg-G)和其他3组腹膜内注射链脲佐菌素200mg/kg以诱导糖尿病状态。在Neg-G中没有蚂蚁处理,而在At-14、At-28和At-56中分别用蚂蚁提取物以14、28和56mg/kg BW的剂量处理24天。然后收集胰腺,准备在放大400倍的显微镜下进行检查。通过评估结构损伤和炎症细胞浸润来确定胰岛损伤的评分。采用Kruskal-Wallis和Mann-Whitney作为统计分析。结果:Mann-Whitney分析表明,At-56对胰岛损伤的评分显著低于Neg-G,p0.05。结论:蚂蚁提取物56mg/kg BW可减轻链脲佐菌素诱导的糖尿病小鼠胰岛损伤。
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