Basel Life Science Week最新文献_第8页

Establishment of the culture condition to maintain the primary Human Colorectal Cancer microenvironment by using a 3D perfusion bioreactor 利用三维灌注生物反应器建立维持人原发性结直肠癌微环境的培养条件

Basel Life Science Week Pub Date : 2015-09-20 DOI: 10.5281/ZENODO.31212

Celeste Manfredonia

引用次数: 0

Binding Studies of Type I, II, and IV Kinase Inhibitors against Abl Kinase using Back-Scattering Interferometry MOA for allosteric inhibitors that overcome drug resistance 利用后向散射干涉法研究I、II和IV型激酶抑制剂与Abl激酶的结合研究

Basel Life Science Week Pub Date : 2015-09-18 DOI: 10.5281/zenodo.31173

R. Lavieri

引用次数: 0

Validation Of A New Optical Method For Ion Channel Screening 离子通道筛选新光学方法的验证

Basel Life Science Week Pub Date : 2015-09-18 DOI: 10.5281/ZENODO.31141

F. Grohovaz

{"title":"Validation Of A New Optical Method For Ion Channel Screening","authors":"F. Grohovaz","doi":"10.5281/ZENODO.31141","DOIUrl":"https://doi.org/10.5281/ZENODO.31141","url":null,"abstract":"Menegon A.1, Agus V.2, Crespi D.2, Rolland J.F.2, Redaelli L.2, Scarabottolo L.2, Seneci P.3, Mazzocchi N.1, Imberti M.4, Poli C., Lanati A.5, Grohovaz F.1,6 1. SanRaffaele Scientific Institute, Milan; 2. Axxam SpA, Bresso (MI); 3. University of Milan, Milan; 4. OPEN Sistemi srl – Cremona; 5. Valore Qualita, Pavia; 6. San Raffaele University, Milan. \u0000\u0000Numerous top-selling drugs are ion-channel modulators. Unfortunately, the screening of candidate molecules for ion channels is mostly based on optical approaches that measure, as final readout, a cascade of events rather than direct channel activation. Overall these approaches are compatible with high throughput requirements but can be poorly predictive. Alternative methods, such as manual or automated patch clamp technologies, are valuable but expensive and time consuming. The poster illustrates the validation of a new Optical Method for Ion Channel Screening patented in Europe and pending acceptance in the USA (EP2457088, WO2011009825, US13/386225). This innovative approach, based on the study of ion channel conductance by a fluorescence-based approach, was implemented in a fully automated system built according to EC standards. When this platform was tested on different ion channel targets, the results were in good agreement with those reported in literature by gold standard approaches (manual and automated patch clamp). Finally, the platform was challenged with a small library of candidate molecules acting on the P2RX7 ion channel and the results were compared with those obtained by FLIPR and QPatch. Also in this case, the results were comparable with those obtained by the established screening approaches. Overall, we demonstrate that this new platform allows the study of different ion channels, independently of the way they are operated, of their specific permeability, and of the extent of the ionic flux sustained at rest. This innovative platform provides an excellent compromise of cost per data point, throughput and prediction power in drug screening.","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129962026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating Gene Synthesis and Microfluidics for Rapid Protein Engineering 整合基因合成和微流体技术用于快速蛋白质工程

Basel Life Science Week Pub Date : 2015-09-18 DOI: 10.5281/ZENODO.31172

M. Blackburn

引用次数: 0

Adenosine A1 and A2A receptors as possible biomarkers in peripheral cells from idiopathic normal-pressure hydrocephalus patients 特发性常压脑积水患者外周血细胞中腺苷A1和A2A受体可能的生物标志物

Basel Life Science Week Pub Date : 2015-09-18 DOI: 10.5281/ZENODO.31148

B. Arosio

引用次数: 0

Using signaling pathway activation analysis to identify prospective drugs that may slow down or reverse the effects of skin aging and photo-aging 利用信号通路激活分析确定可能减缓或逆转皮肤老化和光老化影响的前瞻性药物

Basel Life Science Week Pub Date : 2015-09-18 DOI: 10.5281/ZENODO.31135

Polina Mamoshina, A. Moskalev, A. Zhavoronkov, A. Aliper, A. Buzdin, Evgenia Schastnaya, Artem V. Artemov