Journal of Food and Pharmaceutical Sciences最新文献

{"title":"Determination of Aflatoxins in Feed by HPLC and PCR","authors":"Areej Zuhair Azeez, Mrwa Thamer Hindi, Maha Muhamed Khudiar, Adel Saadi Al Saadi, N. Khadim","doi":"10.22146/JFPS.881","DOIUrl":"https://doi.org/10.22146/JFPS.881","url":null,"abstract":"The aim of this study is to identify aflatoxin secretion isolates in animal feeds by using HPLC and PCR  methods . In this study we collected fourty three samples of animal feed from different sites in Iraq (maize ,soybean ,sunflower grain ,barley grain, wheat). we isolated fungi  on potato dextrose agar, Aspergillus flavus  fungi was isolated from this samples  and identified the enzyme activities were tested for this isolate. The detection and determination for aflatoxin secretion of the isolates were done by using High Performance Liquid Chromatography (HPLC) technique. Twelve isolates shown Aflatoxin B1 secretion. Polymrease chain reaction ( PCR) technique is an alternative method to detect for Aspergillus spp. strains that secret aflatoxin by using specific primers( ITS1) endogenous gene for  Aspergillus flavus  and (ord , nor) genes  for aflatoxin B1 secreation, the PCR technique  considered to be an important role for safety and quality in industrial food and feed.","PeriodicalId":314392,"journal":{"name":"Journal of Food and Pharmaceutical Sciences","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130204849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Simple Liquid-Liquid Fractionation (LLF) Method for Isolating Deoxyandrographolide dan Andrographolide from Herbs of Andrographis paniculata (Burm., F) Ness and Its Cytotoxic Activity on 3T3-L1 Preadipocyte Cells","authors":"P. Novitasari, Novi Tri Astuti, S. Pramono, R. Tjandrawinata, A. Nugroho","doi":"10.22146/JFPS.875","DOIUrl":"https://doi.org/10.22146/JFPS.875","url":null,"abstract":"The main bitter constituents of sambiloto (Androgaphis paniculata (Burm., F) Ness) are diterpene lactones, namely andrographolide and deoxyandrographolide which have been reported to have antidiabetic, cytotoxic, antiatherosclerosis, anti-inflammatory, and antimicrobial activity. There are many studies that performed the isolation of deoxyandrographolide and andrographolide from A. paniculata herbs, but most of them included several steps that make them not efficient. This research was conducted to do an isolation of deoxyandrographolide and andrographolide through liquid-liquid fractionation (LLF) due to its simplicity, low cost, and time efficient. The extraction of deoxyandrographolide and andrographolide from the herbs was carried out using chloroform as the solvent by using Soxhlet apparatus, and LLF was performed to isolate the compounds. The identities of the compounds were confirmed by TLC scanner compared to its standard references. Hence, these present methods were successfully isolated and determined deoxyandrographolide and andrographolide of A. paniculata. The compounds were also showed relatively moderate cytotoxicity on 3T3-L1 cell lines using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay, with LC50 of deoxyandrographolide and andrographolide; 29.3173 µg/mL and 37.7011 µg/mL, respectively.","PeriodicalId":314392,"journal":{"name":"Journal of Food and Pharmaceutical Sciences","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120961429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Ultrasonic-Assisted Fractionation with Full-Factorial Design for the Production of Alkaloid-Rich Fraction from Ficus septica Leaves","authors":"M. H. Ridho, A. P. Gani, S. Wahyuono, N. Fakhrudin","doi":"10.22146/JFPS.852","DOIUrl":"https://doi.org/10.22146/JFPS.852","url":null,"abstract":"Ficus septica Burm. F (Awar-awar) in Indonesian herbal medicine traditionally used for the treatment of various disease. Previous studies indicated that the F. septica leaves contained phenantroindolizidine alkaloids with anticancer activity. Fractionation of the ethanol extract of F. septica leaves using n-hexane was able to separate chlorophylls and other inert non–polar constituents from the extract. This fractionation process can be optimized using ultrasonic-assisted fractionation to yield n-hexane insoluble fraction (HIF) that is rich in alkaloids. This study aimed to produce HIF from the ethanol extract of F. septica leaves by using optimized ultrasonic-assisted fractionation with n-hexane as a solvent and full factorial as experimental design. Ficus septica leaves were macerated in ethanol (60%) and the extract was used for the ultrasonic-assisted fractionation process. The duration of fractionation (5-30 minutes), the extract : n-hexane ratios (1:0.1 - 1:1.66 ml/ml), and the ultrasonic power (21-106 Wrms) were optimized to determine the optimal condition for each variable. These optimal variables were used for the production of HIF from the extract by using full factorial design in the ultrasonic-assisted fractionation process. The total alkaloid content was measured using spectrophotometry and was used as parameter for the optimization process. We found that the optimal condition for the fractionation process based on each single variable optimization were 5 minutes of the fractionation duration, 66-70Wrms of the ultrasonic power, and 1:0.7 - 1:0.8 of the extract : n-hexane ratio. By using these optimized variables, the ultrasonic-assisted fractionation using full factorial design yielded up to 0.035% total alkaloid content which is almost double from those in the extract (0.019%). This study provided a basic experimental model for the production of alkaloid-enriched HIF from F. septica leaves in the industrial or pilot scale, and might contribute to the development of Indonesian herbal medicine products from natural resources.","PeriodicalId":314392,"journal":{"name":"Journal of Food and Pharmaceutical Sciences","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125931930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Methanolic Extract and Ethyl Acetate Fraction of Kepel Fruit (Stelechocarpus burahol) to α-Glutathione S-Transferase Enzyme Concentration of Rat Liver and Blood Induced by CCl4","authors":"Y. Rabbani, C. M. Airin, S. Riyanto","doi":"10.22146/jfps.691","DOIUrl":"https://doi.org/10.22146/jfps.691","url":null,"abstract":"Degenerative diseases are mostly cause by high free radical concentrations in the body. Kepel fruit (Stelechocarpus burahol) is known to contain flavonoid compounds, a class of compounds that has free radical scavenging activity in the body and could affect the activity of antioxidant enzymes. There have been reports that methanolic extract and ethyl acetate fractions of Kepel fruit have the lowest IC50 values, compared to extracts or fractions which are made using other organic solvents, on in vitro assays so that flavonoids are suspected as responsible compounds to the free radical scavenging activity. This study aimed to identify the presence of flavonoid content in methanolic extract and the fraction of ethyl acetate of Kepel fruit and also its effect on rat alpha Glutathione S-Transferase (α-GST) enzyme concentration for the function to prevent hepatic cell damage due to carbon tetrachloride exposure. The identification of flavonoids content used a series of color chemical reaction tests and thin layer chromatography that used silica gel 60F254 as the solid phase and mixture of ethyl acetate, formic acid, glacial acetic acid, and water (100: 11 : 11: 20 v/v) as the mobile phase. This study used 30 Sprague-Dawley female rats (age 2-3 months) that were grouped into 5 groups as solvent control (CMC-Na) group, negative control (CCl4) group, positive control (vitamin C) group, methanolic extract group, and ethyl acetate fraction group. All groups were induced with carbon tetrachloride (CCl4) compounds except for the CMC-Na solvent control group. Blood sampling at 0 h, 24 h, and 72 h, while liver sampling at 24 h and 72 h, with 3 samples each group. The analysis of rat α-GST enzyme concentrations used the Enzyme-linked Immunosorbent Assay (ELISA) method. Based on the phytochemical test, the methanolic extract and the ethyl acetate fraction of Kepel fruit have been identified contains flavonoids compound. Based on the in vivo study, the positive control group's α-GST enzyme concentration was decreased, while the negative control group's α-GST enzyme concentration was increased. Administration of methanolic extract and ethyl acetate fraction also decreased both liver and blood rat α-GST enzyme concentrations, although without significant correlation, and still could prevent the hepatic cell damage due to carbon tetrachloride exposure.","PeriodicalId":314392,"journal":{"name":"Journal of Food and Pharmaceutical Sciences","volume":"306 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116226010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of Purple Sweet Potato Yogurt and Its Effect on Lipid Profiles of Sprague Dawley Rats Fed with High Fat Diet","authors":"L. Lestari, Qisthira Swasti Amirina, Dina Septari Anindyah, A. Nisa, A. N. Huda, Widiyani Indrianingrum, Prasetyastuti, Nurliyani","doi":"10.22146/jfps.679","DOIUrl":"https://doi.org/10.22146/jfps.679","url":null,"abstract":"The objectives of this study were to evaluate the effect of purple sweet potato (PSP) yogurt on the lipid profile of Sprague Dawley rats. The PSP yogurt was made from fresh PSP (F-PSP) and its powder (P-PSP). The viscosity, pH, total titratable acid, proximate, antioxidant activity, anthocyanin content, and total lactic acid bacteria (LAB) between those yogurts were compared. Selected yogurt was given to Sprague Dawley rats for 4 weeks. Serum lipid profiles were determined after 2 and 4 weeks of treatment. F-PSP yogurt has better physical, chemical and microbiological properties compared to P-PSP yogurt. The serum cholesterol and triglyceride levels in F-PSP yogurt group was not significantly different compared to control group (P>0.05). However, the reduction of serum LDL cholesterol in F-PSP yogurt group was significantly higher than control group. The serum HDL cholesterol in the yogurt group decreased after 2 weeks of treatment, but it increased after 4 weeks of treatment. F-PSP yogurt could be used as an alternative functional food that could lower the blood cholesterol level because of the ability to reduce the LDL cholesterol level.","PeriodicalId":314392,"journal":{"name":"Journal of Food and Pharmaceutical Sciences","volume":"303 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128626568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Profile and Observing Honey Adulteration Using Fourier Transform Infrared (FTIR) Spectroscopy and Multivariate Calibration","authors":"S. Prabowo, Yudha Agus Prayitno, Yuliani","doi":"10.22146/jfps.653","DOIUrl":"https://doi.org/10.22146/jfps.653","url":null,"abstract":"Honey is a complex mixture that contains nutrients and bioactive composition and chemical compositionthat is needed by the human body. Honey is also rich in antioxidants because it is prone to falsification given its manyproperties. This study discusses the chemical and nutritional profiles and the observation of counterfeiting in honey usingthe infrared septicroscopy method. The honey used is obtained from providers of native honey from Kalimantan forestswith 3 kinds of honey brands, namely Mahuka A (MHA), Mahuka B (MHB) and Mahuka C (MHC). Tests of chemicaland nutritional profiles included Water content, ash content, protein content, fat content and calcium levels followed byhoney counterfeiting observation using the Fourier Transform Infrared (FTIR) method with a combination of Partial leastsquare (PLS) calibration model and Principle Component Regression (PCR). The results of all honey samples havevarying values in the water content between 26.75 - 31.00%. Ash content of 0.10 -0.16%. Protein content 0.24 - 0.88%fat content 0.08 -0.44% carbohydrate content. Observation of FTIR honey counterfeiting is used to replace the authenticity of Honey (MH). FTIR combined with Partial Least Square (PLS) was optimized in the subsequent testing ofa mixture of sucrose (MCS) with native honey (MH). Calibration models were taken in a combination of regions 1423 -1825 cm-1. A high coefficient of determination (R2) of 0.9960 with a calibration value (RMSEC) of the root error of thesquare root low of 0.0898% v / v was successfully understood in the MHA on the PLS model. high R2 values and lowRMSEC and RMSEP values on calibration and validation assessments with both accuracy and precision models used.","PeriodicalId":314392,"journal":{"name":"Journal of Food and Pharmaceutical Sciences","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125128900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemometrics Application for Grouping and Determinating Volatile Compound which related to Antioxidant Activity of Turmeric Essential Oil (Curcuma longa L)","authors":"Rosy Islamadina, A. Can, A. Rohman","doi":"10.22146/jfps.658","DOIUrl":"https://doi.org/10.22146/jfps.658","url":null,"abstract":"Turmeric essential oil is known to have antioxidant activity. Various in vitro antioxidantactivity assays has been carried out. Related to this research, it tries to examine the antioxidantpotential of turmeric essential oil and see the composition that is responsible for antioxidant activitycombine with chemometrics. The research method used was a narrative review of 60 articlesobtained from several databases. The review conducted on profiling essential oil compounds thatidentified using GC-MS and evaluation of the antioxidant activity of turmeric essential oil with themost commonly used method including scavenging radical 2,2 diphenyl-1-picrylhydrazyl (DPPH)and 2,2-azinobis (3-ethylbenzothiazoline-6 sulfonic acid (ABTS). Analysis methods used forgrouping various multivariate data subjects and determaining the relationship between thevariables were Principal Component Analysis (PCA) and Cluster Analysis (CA). According to thereview, turmeric essential oils are proven to have potential antioxidant activity and have variationsin chemical contents. PCA was success for grouping subjects with various correlated variables,determining variables wich the most influential and correlation between variables. CA method canbe used to group samples without requiring mutually correlated variables.","PeriodicalId":314392,"journal":{"name":"Journal of Food and Pharmaceutical Sciences","volume":"157 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115991449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total Flavonoid Content and Antioxidant Activity of Temu Mangga (Curcuma mangga Val. & Zijp) and its Classification with Chemometrics","authors":"Yuniar Intan Hartono, Indah Widyastuti, Hanna Zaidah Luthfah, Rosy Islamadina, A. Can, A. Rohman","doi":"10.22146/jfps.650","DOIUrl":"https://doi.org/10.22146/jfps.650","url":null,"abstract":"Indonesia is a rich country in terms of its biodiversity. Herbs that are widely used tomaintain a healthy body comes from the Zingiberaceae family, including temu mangga. Temu mangga(Curcuma mangga Val. & Zijp) is effective for relieving gastric pain, diarrhea, menstrual pain, acneand ulcers, and increasing appetite. Besides, temu mangga is considered to have antioxidant activitydue to the presence of the phenolic and flavonoid compounds. This study aims to determine thecorrelation and classification of temu mangga from various places based on total flavonoid contentand antioxidant activities. The samples were extracted with methanol, then tested for totalflavonoid content by AlCl3 method and antioxidant activity by DPPH free radical capture method.Analysis was performed using correlation coefficient value and chemometrics PCA (PrincipalComponent Analysis) and CA (Cluster Analysis) to see the relationship between total flavonoidcontent with their antioxidant activity and to see their classification. Total flavonoid content ofmethanolic extract of temu mangga gives a moderate correlation to its antioxidant activity with r =-0.6085 and clustering samples successfully determined by PCA and CA which resulted in 4 groups.Keywords: Curcuma mangga Val. & Zijp, Methanolic Extract, Flavonoid, Antioxidant, PCA, CA","PeriodicalId":314392,"journal":{"name":"Journal of Food and Pharmaceutical Sciences","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134144012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Profile of Anticancer Activities of Sambiloto Extract (Andrographis paniculata Nees) from Several Locations in East Java","authors":"R. Mutiah, Fina Luthfiana, Arief Suryadinata, Wirda Anggraini, Anik Listiyana","doi":"10.22146/jfps.647","DOIUrl":"https://doi.org/10.22146/jfps.647","url":null,"abstract":"Sambiloto (Andrographis paniculata Nees) contains andrographolid compound which has potentialas an anticancer. The difference of growth location has affected the distinction of metabolite content. Thisstudy aims to determine the profile of anticancer activities of A. paniculata extract from several locations inEast Java. The extracts from several locations were tested for activity on T47D cancer cell using MTT assaymethod. Thus, the result of this study describes that A. paniculata extract from several different locations doesnot provide significantly different anticancer activities with the amount of (p>0.05). The extracts which havemoderate anticancer activity were A. paniculata extracts from Selopuro Subdistrict Blitar Regency, SingosariSubdistrict Malang Regency, Lowokwaru Subdistrict Malang City, Klojen Subdistrict Malang City,Purwodadi Subdistrict Pasuruan Regency, Purwosari Subdistrict Pasuruan Regency, and PandaanSubdistrict Pasuruan Regency","PeriodicalId":314392,"journal":{"name":"Journal of Food and Pharmaceutical Sciences","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124014547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Liquid Soap Containing Bentonite and Combination of Corn Oil and Virgin Coconut Oil For Cleansing Najs Mughalladzah","authors":"Chairunnisa Mahdi Pratama, Astri Desmayanti, Marchaban, A. Rohman","doi":"10.22146/jfps.640","DOIUrl":"https://doi.org/10.22146/jfps.640","url":null,"abstract":"This research was intended to formulate liquid bentonite soap using combination of corn oil andvirgin coconut oil used for cleansing najs mughalladzah (extreme najs). five formula of soap designated with I-Vapplying bentonite and combination of VCO and CO with different concentrations. The assessment of sopquality was based on organoleptic evaluation, foam stability, density, pH-value, and total of active component.Evaluation results were analyzed using simplex lattice design (SLD) to obtain optimum formula. Verificationof optimum formula was analyzed using one sample t-test with level of significance of 0.05. The resultsshowed that optimum concentration of bentonite was 6.5% with percentages of corn oil 85.43% and virgincoconut oil of 14.57%. Based on one sample t-test, there is no significant difference (pH > 0.05) from pH-valueresponse and foam stability between the predicted optimum formula as analyzed using SLD and actualformula. Bentonite liquid soap which is formulated using combination of corn oil and virgin coconut oilaccording to National Standardization Body SNI 2588:2017 so that the soap formula could be applied forcleansing najs mughalladzah.","PeriodicalId":314392,"journal":{"name":"Journal of Food and Pharmaceutical Sciences","volume":"131 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123168260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}