Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery最新文献_第3页

Inhibitory effects on human neutrophil bursts of raw, grilled and boiled eggplant (Solanum melongena L.) - 生、烤、煮茄子对人中性粒细胞爆发的抑制作用

Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery Pub Date : 1900-01-01 DOI: 10.5455/SPATULA.20110616110705

P. Braga, Yan Yan Wang, M. Sasso, M. Culici, Paola Verducci, M. Fibiani, R. L. Scalzo

{"title":"Inhibitory effects on human neutrophil bursts of raw, grilled and boiled eggplant (Solanum melongena L.) -","authors":"P. Braga, Yan Yan Wang, M. Sasso, M. Culici, Paola Verducci, M. Fibiani, R. L. Scalzo","doi":"10.5455/SPATULA.20110616110705","DOIUrl":"https://doi.org/10.5455/SPATULA.20110616110705","url":null,"abstract":"AIM: This study investigated the effects on human cells of grilled, boiled and raw eggplant aqueous extracts on the release of reactive oxygen and nitrogen species (ROS and RNS) during respiratory bursts of human polymorphonuclear neutrophils (PMNs). \u0000METHODS: Luminol-amplified chemiluminescence (LACL) was used used to assess the concentrations (ranging from 160 μg/ml to 0.62 μg/ml) at which there was residual biological activity. The respiratory bursts were analysed in two different experiments. The first evaluated the action of the extracts on the cell release of ROS (mainly superoxide and hydroxyl radicals); in the second, L-Arginine (L-Arg) was added to the medium as a nitric oxide (NO) donor before the challenge in order to study the possible quenching reaction of the extracts on the generation of NO and peroxynitrite as by-products of the rapid interaction between O2¯• and NO. \u0000RESULTS: Comparison of the antioxidant effects showed that the behaviour of the three extracts was significantly different. Both of the both cooked samples had greater antioxidant effects than the raw sample in both experiments, and these effects were 10-12% greater after the addition of L-Arg. \u0000CONCLUSIONS: These findings are in line with those indicating that cooking eggplant increases the principal phenolic compounds (such as chlorogenic and caffeic acids), which are known to have antioxidant activity. This effect is interesting for the strategy of improving the antioxidant charge and restoring redox balance in human cells when the stress generated favours free radicals as a result of a decrease in antioxidant levels.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132798180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Acupressure Practice and Dyspnea in Patients with Lung Cancer 指压疗法与肺癌患者呼吸困难的关系

Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery Pub Date : 1900-01-01 DOI: 10.5455/spatula.20160426022110

N. Doğan, S. Taşcı

引用次数: 2

Use of herbal pharmaceutical products in Al-Taif area, Saudi Arabia: prevalence and indication 沙特阿拉伯Al-Taif地区草药产品的使用:流行情况和适应症

Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery Pub Date : 1900-01-01 DOI: 10.5455/SPATULA.20140924112840

A. M. A. A. El-Mawla, A. Albarrag, M. Abdallah

引用次数: 3

Complementary and alternative medical methods: attitude, knowledge and use by health personnel - 补充和替代医疗方法:保健人员的态度、知识和使用

Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery Pub Date : 1900-01-01 DOI: 10.5455/spatula.20160313010209

Tulay Sagkal Midilli, Ebru Baysal, Gulzade Akarsu, E. Korkmaz

{"title":"Complementary and alternative medical methods: attitude, knowledge and use by health personnel -","authors":"Tulay Sagkal Midilli, Ebru Baysal, Gulzade Akarsu, E. Korkmaz","doi":"10.5455/spatula.20160313010209","DOIUrl":"https://doi.org/10.5455/spatula.20160313010209","url":null,"abstract":"Aim: The aim of this study was to determine the attitude of health personnel to CAM methods, and their knowledge and use of them. Methods: The study was descriptive in nature. It was conducted between March and June 2014 with 202 health workers employed at two government hospitals in the province of Manisa, Turkey. Results: It was found that 74.8% of the health workers used CAM methods, 81.7% had a medium to good knowledge of them, and more than half had positive attitudes towards them. The three CAM methods which the participants knew about and used the most were, in order, the use of plants, showers, and hot and cold treatment. Health personnel differed in their attitudes to CAM methods according to their profession, educational level and gender (p< .05). Conclusion: Health personnel had a high level of knowledge, they had positive attitudes towards CAM methods and most of them used at least one CAM method. Doctors, those with a higher educational level and female health workers had greater knowledge of CAM, while nurses and female health workers had a higher opinion of CAM.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115719927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Larvicidal activity of essential oils from spices sold at Kariakoo market in Dar es Salaam, Tanzania, against Anopheles gambiae Giles ss and Culex quinquefasciatus Say 坦桑尼亚达累斯萨拉姆Kariakoo市场销售的香料精油对冈比亚按蚊和致倦库蚊的杀幼虫活性

Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery Pub Date : 1900-01-01 DOI: 10.5455/spatula.20160613034220

D. Runyoro, O. Ngassapa, E. Innocent, Raphael Zozimus Sangeda, Thomas Lukuba

引用次数: 1

Role of propolis in improving male rat fertility affected with aluminum chloride cytotoxicity 蜂胶对氯化铝细胞毒性影响的雄性大鼠生育能力的改善作用

Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery Pub Date : 1900-01-01 DOI: 10.5455/SPATULA.20111122065544

A. Mahran, Ahmed Hassan Al-Rashidy, A. M. A. A. El-Mawla

{"title":"Role of propolis in improving male rat fertility affected with aluminum chloride cytotoxicity","authors":"A. Mahran, Ahmed Hassan Al-Rashidy, A. M. A. A. El-Mawla","doi":"10.5455/SPATULA.20111122065544","DOIUrl":"https://doi.org/10.5455/SPATULA.20111122065544","url":null,"abstract":"BACKGROUND: Aluminum chloride (AlCl3) is commonly used in daily life but it can be induce reproductive toxicity. Propolis has been reported to be important antioxidant. Therefore, the present study aimed to investigate the protective effects of propolis against reproductive toxicity of aluminum chloride (AlCl3) in male rats. \u0000METHODS: 60 male albino rats were divided into three equal groups, the first served as negative control, the second received AlCl3 (34 mg/kg bw, 1/ 25 LD 50), the third received AlCl3 and treated with propolis (50mg/kg d.w.) .Treatment was continued for 70 days. \u0000RESULTS: AlCl3 caused a decrease in body and testes weights and testosterone hormone. In addition, histological changes as damages within the seminiferous tubules and vascular degeneration of the germ cells and Sertoli cells cytoplasm were observed. On the other hand, electron microscopy study showed changes in the testis seminiferous tubules such as atrophy of the tubular membrane, mitochondria, endoplasmic reticulum, Golgi apparatus and nucleus. Our results revealed that propolis alleviated the reproductive toxic effects of AlCl3. \u0000CONCLUSION: Treatment with propolis alleviates AlCl3-associated hazards and protects the testicular tissues from AlCl3 toxicity.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125819317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Antimycobacterial, antioxidant activity and toxicity of extracts from the roots of Rauvolfia vomitoria and R. caffra - 牛蒡根和牛蒡根提取物的抑菌、抗氧化活性及毒性研究

Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery Pub Date : 1900-01-01 DOI: 10.5455/SPATULA.20110514043359

P. Erasto, Z. Mbwambo, R. R. Nondo, N. Lall

{"title":"Antimycobacterial, antioxidant activity and toxicity of extracts from the roots of Rauvolfia vomitoria and R. caffra -","authors":"P. Erasto, Z. Mbwambo, R. R. Nondo, N. Lall","doi":"10.5455/SPATULA.20110514043359","DOIUrl":"https://doi.org/10.5455/SPATULA.20110514043359","url":null,"abstract":"ABSTRACT \u0000AIM: This study aimed to investigate the antimycobacterial activity of total alkaloid and ethanolic aqueous extracts of Rauvolfia vomitoria and Rauvolfia caffra. It further aimed to evaluate antioxidant activity and cytotoxicity effect of the extracts which would have shown high antimycobacterial efficacy. \u0000METHODS: The alkaloids from the roots of two plant species were extracted according to standard procedures. Antimycobacterial activity of the extracts was evaluated using a two fold microdilution method. The antioxidant activity of the alkaloids and ethanolic extracts was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing capacity assays. The potential cytotoxicity of the extracts was evaluated using brine shrimp toxicity assay. Comparison of alkaloidal composition of the two alkaloidal extracts was done using HPLC analysis. \u0000RESULTS: The study revealed that, alkaloid extracts from the roots of Rauvolfia species had higher antimycobacterial activity against Mycobacterium madagascariense (MM) and M. indicus pranii (MIP). The MIC values of alkaloid extracts ranged between 0.078 to 1.25 mg/ml, while the ethanolic extracts were generally moderately active against test organism. The alkaloids and ethanolic aqueous extracts of R. vomitoria exhibited high antioxidant activity. In the cytotoxicity assay, extracts did not exhibit any toxicity against brine shrimps. \u0000CONCLUSION: The alkaloids from the roots of R. vomitoria have high antimycobacterial and antioxidant activity. The alkaloids ability to inhibit the growth of two Mycobacterium strains may be associated to its antioxidant activity. The cytotoxicity of the most active extract is negligble when compared to the standard anticancer drug, this may explain why R. vomitoria is highly used in the treatment of various infectious and physiological ailments.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128950758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Antimicrobial and cytotoxicity activity of selected Pare folk medicinal plants Pare民间药用植物抑菌活性及细胞毒活性

Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery Pub Date : 1900-01-01 DOI: 10.5455/SPATULA.20160418065130

E. Godfrey, L. Chauka, M. Chacha

{"title":"Antimicrobial and cytotoxicity activity of selected Pare folk medicinal plants","authors":"E. Godfrey, L. Chauka, M. Chacha","doi":"10.5455/SPATULA.20160418065130","DOIUrl":"https://doi.org/10.5455/SPATULA.20160418065130","url":null,"abstract":"Aim: The study aimed to evaluate antimicrobial and cytotoxicity of Grewia villosa , Cassia abbreviata and Hoslundia opposita . Methods: Microdilution method and brine shrimp lethality test were employed to evaluate antimicrobial and cytotoxicity of plant extracts. Results: The extracts of G. villosa , C. abbreviata and H. opposita showed different levels of antimicrobial activities on Gram negative bacterial and fungal species tested. The minimum inhibition concentration (MIC) ranges of G. villosa , C. abbreviata and H. opposita extracts were 0.391-25, 0.195-25 and 0.391-25 mg/mL respectively. Cassia abbreviata stem methanolic extracts and roots ethyl acetate extracts exhibited the highest activity with minimum inhibition concentration (MIC) of 0.195 mg/mL against S. typhi and K. oxytoca respectively. At least one of the extract from each plant exhibited a cytotoxicity nature when tested on brine shrimps where the G. villosa stem chloroform extract was the most cytotoxic with fifty percent lethal concentration (LC50) of 48.432 µg/mL. Conclusion: The findings in this study validate the traditional use of G. villosa , C. abbreviata and H. opposita in management of bacterial and fungal infections. Further phytochemical investigations on G. villosa , C. abbreviata and H. opposita growing in Pare Mountains are recommended to contribute to drug development.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130841433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antiplasmodial activities of limonoids from Entandrophragma angolense ( Meliaceae ), and their semi-synthetic derivatives: Study of the structure-activity relationship. 安哥莲柠檬素及其半合成衍生物的抗疟原虫活性研究。

Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery Pub Date : 1900-01-01 DOI: 10.5455/SPATULA.20130516064018

G. R. Tchouya, J. Bickii, Timolon Andzi Barh, F. Boyom, Bruno Lenta Djakou, J. Tchouankeu

{"title":"Antiplasmodial activities of limonoids from Entandrophragma angolense ( Meliaceae ), and their semi-synthetic derivatives: Study of the structure-activity relationship.","authors":"G. R. Tchouya, J. Bickii, Timolon Andzi Barh, F. Boyom, Bruno Lenta Djakou, J. Tchouankeu","doi":"10.5455/SPATULA.20130516064018","DOIUrl":"https://doi.org/10.5455/SPATULA.20130516064018","url":null,"abstract":"SUMMARY AIM: In the aim to understand the structure-activity relationship of some natural antimalarial agents, two limonoids 7α- acetoxydihydronomilin 1 and 7α- obacunyl acetate 2 obtained from E. angolense and two new semi-synthesized limonoids 7α- acetoxy-14, 15-deepoxynomilin 3 and 1, 2- dihydro-1, 4-dihydroxy-6-deoxoatalantin 4 obtained by partial synthesis from 7α- acetoxydihydronomilin 1 were evaluated for their in vitro activity against the W2 strain of Plasmodium falciparum . METHODS: 7α- acetoxydihydronomilin 1 and 7α- obacunyl acetate 2 were obtained after chromatographic separations of the plant extract on silica gel columns. 7α- acetoxy-14, 15-deepoxynomilin 3 was obtained by reduction of 7α- acetoxydihydronomilin 1 with zinc-copper couple and 1,2-dihydro-1,4-dihydroxy-6-deoxoatalantin 4 was obtained by hydrolysis of 7α- acetoxydihydronomilin 1 with a KOH solution. The structural determinations of the compounds were made through NMR (Nuclear Magnetic Resonance) spectroscopy analysis. To assess antimalarial activities, different dilutions of the compounds were incubated at 37°C with cultured W2-strain of P. falciparum parasites for 48 hours. Parasites were thereafter fixed and stained, and parasitemias of treated and control cultures were determined. Results were means, compared to untreated controls, from 3 experiments. RESULTS: 7α- obacunyl acetate 2 was found to be the most active in vitro against P. falciparum parasites, with an IC 50 of. 5.14 µM. 7α-acetoxydihydronomilin 1 , 7α- acetoxy-14, 15-deepoxynomilin 3 and 1, 2- dihydro-1, 4-dihydroxy-6-deoxoatalantin 4 were found to be less active. CONCLUSION: Although the presence of α,β–unsaturated ketone is an important feature for enhanced antiplasmodial activity of limonoids, the localisation of α,β–unsaturated carbonyl moiety in ring A seems to be critical for the enhancement of that activity.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123324911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Protective effect against hydroxyl-induced DNA damage and antioxidant activity of Radix bupleuri in vitro - 柴胡对羟基诱导DNA损伤的保护作用及体外抗氧化活性研究

Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery Pub Date : 1900-01-01 DOI: 10.5455/SPATULA.20121209013827

Xican Li, Meinan Xu, Dongfeng Chen

{"title":"Protective effect against hydroxyl-induced DNA damage and antioxidant activity of Radix bupleuri in vitro -","authors":"Xican Li, Meinan Xu, Dongfeng Chen","doi":"10.5455/SPATULA.20121209013827","DOIUrl":"https://doi.org/10.5455/SPATULA.20121209013827","url":null,"abstract":"AIM: As a typical Chinese herbal medicine, Radix bupleuri (RB) possesses various pharmacological effects involved in antioxidant ability. However, its antioxidant ability has not been explored so far. The aim of the study was to investigate its antioxidant ability, then further discuss the antioxidant mechanism. \u0000METHODS: RB was extracted by ethanol to obtain ethanolic extract of Radix bupleuri (ERB). ERB was then determined by various antioxidant methods, including DNA damage assay, DPPH assay, ABTS assay, Fe3+-reducing assay and Cu2+-reducing assay. Finally, the contents of total phenolics and total flavonoids were analyzed by spectrophotometric methods. \u0000RESULTS: Our results revealed that ERB could effectively protect against hydroxyl-induced DNA damage (IC50 498.25 µg/mL). In addition, ERB could scavenge DPPH• radical (IC50 909.32 µg/mL) and ABTS+• radical (IC50 51.69 µg/mL), reduce Fe3+ (IC50 418.78 µg/mL) and Cu2+ (IC50 126.83 µg/mL). Chemical analysis demonstrated that the contents of total phenolics and flavonoids in ERB were 12.29 and 77.24 mg quercetin/g, respectively. \u0000CONCLUSION: Radix bupleuri can effectively protect against hydroxyl-induced DNA damage. The protective effect may be associated with radical-scavenging which is via donating hydrogen atom (H•), donating electron (e). Its antioxidant ability can be mainly attributed to the existence of flavonoids (especially rutin).","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121484690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1