Biophotonics South America最新文献_第4页

Comparison between two portable devices for widefield PpIX fluorescence during cervical intraepithelial neoplasia treatment 两种便携式装置在宫颈上皮内瘤变治疗中的广角PpIX荧光比较

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2180914

F. Carbinatto, N. Inada, Welington Lombardi, Natália Fernandez Cossetin, Cinthia Varoto, C. Kurachi, V. Bagnato

{"title":"Comparison between two portable devices for widefield PpIX fluorescence during cervical intraepithelial neoplasia treatment","authors":"F. Carbinatto, N. Inada, Welington Lombardi, Natália Fernandez Cossetin, Cinthia Varoto, C. Kurachi, V. Bagnato","doi":"10.1117/12.2180914","DOIUrl":"https://doi.org/10.1117/12.2180914","url":null,"abstract":"The use of portable electronic devices, in particular mobile phones such as smartphones is increasing not only for all known applications, but also for diagnosis of diseases and monitoring treatments like topical Photodynamic Therapy. The aim of the study is to evaluate the production of the photosensitizer Protoporphyrin IX (PpIX) after topical application of a cream containing methyl aminolevulinate (MAL) in the cervix with diagnosis of Cervical Intraepithelial Neoplasia (CIN) through the fluorescence images captured after one and three hours and compare the images using two devices (a Sony Xperia® mobile and an Apple Ipod®. Was observed an increasing fluorescence intensity of the cervix three hours after cream application, in both portable electronic devices. However, because was used a specific program for the treatment of images using the Ipod® device, these images presented better resolution than observed by the Sony cell phone without a specific program. One hour after cream application presented a more selective fluorescence than the group of three hours. In conclusion, the use of portable devices to obtain images of PpIX fluorescence shown to be an effective tool and is necessary the improvement of programs for achievement of better results.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131562072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

FT Raman spectroscopy in the study of human teeth under medications demineralization FT拉曼光谱在药物作用下人类牙齿脱矿的研究

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2180782

G. R. de Sant' Anna, E. B. Nascimento, A. Higa, E. A. P. Santos, A. M. Espírito Santo, A. Martin

{"title":"FT Raman spectroscopy in the study of human teeth under medications demineralization","authors":"G. R. de Sant' Anna, E. B. Nascimento, A. Higa, E. A. P. Santos, A. M. Espírito Santo, A. Martin","doi":"10.1117/12.2180782","DOIUrl":"https://doi.org/10.1117/12.2180782","url":null,"abstract":"The in situ study evaluated antihistamine (DA) and bronchodilator(DB) drugs actions on dental enamel using FT Raman spectroscopy. Analysis of pH drugs were permorfed, DA 1.48 and DB 2.90. Enamel (n=24) were analysed by FT - Raman and randomly distributed in control group (CG) and experimental groups (GEA and GEB), specimens fixed in palatine appliances. In CG, dripped 20% sucrose (8Xday/3 min)/7 days. In GEA, 20% sucrose (8Xday/3 min) + drug (4Xday/3 min). In GEB, 20% sucrose (8Xday/3 min) B + drug (4Xday/3 min). FT- Raman analysis was performed again. Data analyzed by Student t test and ANOVA Differences in peak intensity of carbonate (CO3) /phosphate (PO4) (p<0.0286) post challenge in GEB related to baseline. The GEB integrated area of inorganic post challenge (p<0.0349).Organic loss in GEA, area (p<0.0228) and intensity (p<0. 0471) between moments and in GEB, area (p<0.0243) and intensity (p<0.00276). Comparing organic area and intensity difference observed post challenge GC (0,31±0,13) and GEB(0,54±0,13). Area difference between GEA(0,30±0,16) X GEB(0,54±0,13) and intensity difference GC(0,09172±0,04) and GEB(0,16±0,04) and GEA(0,091±0,05) X GEB(0,16±0,04). Significant integrated area CO3/PO4 loss for GC(0,12±0,01)X GEB(0,16±0,02) and GEB X GEA(0,13±0,02). For intensity differences were observed between GC(0,08±0,01) and GEB(0,10±0,01), and the last one and GEA(0,08±0,01) After administration of drugs, reduction in the amount of carbonate, organic and inorganic components were observed, denoting possible demineralization.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132006931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of automated prototype for studying the effect of solar aging on sunglasses 太阳老化对太阳镜影响的自动样机研制

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2181007

L. M. Gomes, L. Ventura

{"title":"Development of automated prototype for studying the effect of solar aging on sunglasses","authors":"L. M. Gomes, L. Ventura","doi":"10.1117/12.2181007","DOIUrl":"https://doi.org/10.1117/12.2181007","url":null,"abstract":"The first Brazilian standard for UV protection sunglasses, NBR15111, was drafted and published in 2003, hitherto a faithful copy (mirror) of European, BSEN1836 standard. From 2010 to 2013 the Laboratório de Instrumentação Oftálmica of the School of Engineering of São Carlos (USP) made contribution in the review and drafting of this standard and the main change so far is on the extension of the UV range analysis for protection of sunglasses, i.e. from 280 - 380nm to 280-400nm. In previous studies, there are indications that ultraviolet protection degrades with use and exposure of sunglasses to natural ultraviolet radiation. Thus, this project aims to build a prototype for irradiating sunglasses lenses, where one of the spectacles will be submitted to the solar simulator; and the other to the prototype. This prototype consists of a panel with cover, which houses 100 lenses arranged in the vertical (user’s) position and which will be irradiated by the sun from sunrise until sunset. The lid opens automatically and should turn towards the sun, so that the lens will always be irradiated facing the sun. Sensors will be installed to close the cover and protect the lenses of undesirable weather conditions and to determine the ultraviolet index to which the lenses are being subjected to. The exposure time and UV index will be recorded and automatic opening or closing the lid may also be interfered by a PC by online software. Previously to irradiation, spectroscopy will be performed and then repeated after every 30 days of exposure.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122910377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparative analysis of gingival phenotype in animal and human experimental models using optical coherence tomography in a non-invasive approach 使用光学相干断层扫描在非侵入性方法对动物和人类实验模型的牙龈表型进行比较分析

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2180856

C. C. Mota, L. Fernandes, L. D. Melo, D. S. Feitosa, Renata Cimões, A. Gomes

{"title":"Comparative analysis of gingival phenotype in animal and human experimental models using optical coherence tomography in a non-invasive approach","authors":"C. C. Mota, L. Fernandes, L. D. Melo, D. S. Feitosa, Renata Cimões, A. Gomes","doi":"10.1117/12.2180856","DOIUrl":"https://doi.org/10.1117/12.2180856","url":null,"abstract":"Imaging methods are widely used in diagnostic and among the diversity of modalities, optical coherence tomography (OCT) is nowadays commercially available and considered the most innovative technique used for imaging applications, in both medical and non-medical applications. In this study, we exploit the OCT technique in the oral cavity for identification and differentiation between free and attached gingiva, as well as determining the gingival phenotype, an important factor to determination of periodontal prognosis in patients. For the animal studies, five porcine jaws were analyzed using a Swept Source SS-OCT system operating at 1325nm and stereomicroscope, as gold pattern. The SSOCT at 1325nm was chosen due to the longer central wavelength, that allows to deeper penetration imaging, and the faster image acquisition, an essential factor for clinical setting. For the patient studies, a total of 30 males and female were examined using the SS-OCT at 1325nm and computer controlled periodontal probing. 2D and 3D images of tooth/gingiva interface were performed, and quantitative measurements of the gingival sulcus could be noninvasively obtained. Through the image analysis of the animals jaws, it was possible to quantify the free gingiva and the attached gingiva, the calculus deposition over teeth surface and also the subgingival calculus. For the patient’s studies, we demonstrated that the gingival phenotype could be measured without the periodontal probe introduction at the gingival sulcus, confirming that OCT can be potentially useful in clinic for direct observation and quantification of gingival phenotype in a non-invasive approach.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115511991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Characterization of probe contact effects on diffuse reflectance spectroscopy measurements 漫反射光谱测量中探针接触效应的表征

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2180917

N. Reistad, Mallory Mayjonade, A. Ahadi, S. Andersson-Engels

引用次数: 4

Effects of infrared laser on the bone repair assessed by x-ray microtomography (μct) and histomorphometry 采用x射线显微断层扫描(μct)和组织形态测量法评价红外激光对骨修复的影响

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2180804

Alessandra Rossi Paolillo, F. R. Paolillo, A. D. da Silva, R. Reiff, V. Bagnato, J. M. Alves

{"title":"Effects of infrared laser on the bone repair assessed by x-ray microtomography (μct) and histomorphometry","authors":"Alessandra Rossi Paolillo, F. R. Paolillo, A. D. da Silva, R. Reiff, V. Bagnato, J. M. Alves","doi":"10.1117/12.2180804","DOIUrl":"https://doi.org/10.1117/12.2180804","url":null,"abstract":"The bone fracture is important public health problems. The lasertherapy is used to accelerate tissue healing. Regarding diagnosis, few methods are validated to follow the evolution of bone microarchitecture. The aim of this study was to evaluate the effects of lasertherapy on bone repair with x-ray microtomography (μCT) and histomorphometry. A transverse rat tibia osteotomy with a Kirchner wire and a 2mm width polymeric spacer beads were used to produce a delayed bone union. Twelve rats were divided into two groups: (i) Control Group: untreated fracture and; (ii) Laser Group: fracture treated with laser. Twelve sessions of treatment (808nm laser, 100mW, 125J/cm2, 50seconds) were performed. The μCT scanner parameters were: 100kV, 100μA, Al+Cu filter and 9.92μm resolution. A volume of interest (VOI) was chosen with 300 sections above and below the central region of the fracture, totaling 601sections with a 5.96mm. The softwares CT-Analyzer, NRecon and Mimics were used for 2D and 3D analysis. A histomorphometry analysis was also performed. The connectivity (Conn) showed significant increase for Laser Group than Control Group (32371±20689 vs 17216±9467, p<0.05). There was no significant difference for bone volume (59±19mm3 vs 47± 8mm3) and histomorfometric data [Laser and Control Groups showed greater amount of cartilaginous (0.19±0.05% vs 0.11±0.09%) and fibrotic (0.21±0.12% vs 0.09±0.11%) tissues]. The negative effect was presence of the cartilaginous and fibrotic tissues which may be related to the Kirchner wire and the non-absorption of the polymeric that may have influenced negatively the light distribution through the bone. However, the positive effect was greater bone connectivity, indicating improvement in bone microarchitecture.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126909216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Blue LED irradiation to hydration of skin 蓝光LED照射对皮肤的水合作用

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2181196

P. F. Menezes, M. B. Requena, R. Lizarelli, V. Bagnato

引用次数: 8

Portable widefield imaging device for ICG-detection of the sentinel lymph node 用于前哨淋巴结icg检测的便携式宽视场成像装置

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2181025

A. B. Govone, P. A. Gómez-García, A. Carvalho, R. Capuzzo, D. Magalhães, C. Kurachi

{"title":"Portable widefield imaging device for ICG-detection of the sentinel lymph node","authors":"A. B. Govone, P. A. Gómez-García, A. Carvalho, R. Capuzzo, D. Magalhães, C. Kurachi","doi":"10.1117/12.2181025","DOIUrl":"https://doi.org/10.1117/12.2181025","url":null,"abstract":"Metastasis is one of the major cancer complications, since the malignant cells detach from the primary tumor and reaches other organs or tissues. The sentinel lymph node (SLN) is the first lymphatic structure to be affected by the malignant cells, but its location is still a great challenge for the medical team. This occurs due to the fact that the lymph nodes are located between the muscle fibers, making it visualization difficult. Seeking to aid the surgeon in the detection of the SLN, the present study aims to develop a widefield fluorescence imaging device using the indocyanine green as fluorescence marker. The system is basically composed of a 780nm illumination unit, optical components for 810nm fluorescence detection, two CCD cameras, a laptop, and dedicated software. The illumination unit has 16 diode lasers. A dichroic mirror and bandpass filters select and deliver the excitation light to the interrogated tissue, and select and deliver the fluorescence light to the camera. One camera is responsible for the acquisition of visible light and the other one for the acquisition of the ICG fluorescence. The software developed at the LabVIEW® platform generates a real time merged image where it is possible to observe the fluorescence spots, related to the lymph nodes, superimposed at the image under white light. The system was tested in a mice model, and a first patient with tongue cancer was imaged. Both results showed the potential use of the presented fluorescence imaging system assembled for sentinel lymph node detection.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127223999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermographic diagnostics to discriminate skin lesions: a clinical study 热成像诊断鉴别皮肤病变:一项临床研究

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2180967

M. D. Stringasci, L. Moriyama, A. G. Salvio, V. Bagnato, C. Kurachi

{"title":"Thermographic diagnostics to discriminate skin lesions: a clinical study","authors":"M. D. Stringasci, L. Moriyama, A. G. Salvio, V. Bagnato, C. Kurachi","doi":"10.1117/12.2180967","DOIUrl":"https://doi.org/10.1117/12.2180967","url":null,"abstract":"Cancer is responsible for about 13% of all causes of death in the world. Over 7 million people die annually of this disease. In most cases, the survival rates are greater when diagnosed in early stages. It is known that tumor lesions present a different temperature compared with the normal tissues. Some studies have been performed in an attempt to establish new diagnosis methods, targeting this temperature difference. In this study, we aim to investigate the use of a handheld thermographic camera to discriminate skin lesions. The patients presenting Basal Cell Carcinoma, Squamous Cell Carcinoma, Actinic Keratosis, Pigmented Seborrheic Keratosis, Melanoma or Intradermal Nevus lesions have been investigated at the Skin Departament of Amaral Carvalho Hospital. Patients are selected by a dermatologist, and the lesion images are recorded using an infrared camera. The images are evaluated taken into account the temperature level, and differences into lesion areas, borders, and between altered and normal skin. The present results show that thermography may be an important tool for aiding in the clinical diagnostics of superficial skin lesions.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"9531 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130586028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Plasmonic enhancement in the photoinactivation of Escherichia Coli using rose bengal and gold nanoparticles 用玫瑰红和金纳米粒子增强等离子体对大肠杆菌的光失活

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2180947

H. Kagel, Julia Honselmann Genannt Humme, E. Rosa, R. Turchiello, Arandi Ginane Bezerra Junior

{"title":"Plasmonic enhancement in the photoinactivation of Escherichia Coli using rose bengal and gold nanoparticles","authors":"H. Kagel, Julia Honselmann Genannt Humme, E. Rosa, R. Turchiello, Arandi Ginane Bezerra Junior","doi":"10.1117/12.2180947","DOIUrl":"https://doi.org/10.1117/12.2180947","url":null,"abstract":"In the present study we report on the ability of gold nanoparticles (AuNP) to enhance the antimicrobial activity of the photosensitizer Rose Bengal (RB), a very effective singlet oxygen generator. Our experiments were conducted using a suspension of Escherichia Coli in the presence of either RB or a combination of RB and AuNP. Nanoparticles were synthesized by laser ablation in water, which allows high purity, biologically friendly AuNP production, as compared to traditional chemical methods. Several relative concentrations of bacteria, photosensitizes and AuNP were studied. Bacterial survival rates were determined before and after LED light illumination. The phototoxicity of RB with and without AuNP was checked following illumination for 10 and 20 minutes. As a control, the dark toxicity of RB was verified. The results show that the survival rate of bacteria decreases significantly with the increase of RB concentration and illumination time, which is in accordance with previous works. Interestingly, our results also indicate a significant increase in the lethal photosensitization of RB in the presence of AuNP. We propose this effect is due to plasmonic light enhancement, considering the superposition of RB and AuNP absorption spectra, which favors electric field enhancement effects in the presence of AuNP. Similar experiments using the photosensitizer Methylene Blue (MB) allowed us to test our hypothesis for MB did not show any difference in its phototoxicity in the presence of AuNP. We propose this observed synergistic effect could be an effective way for improving photodynamic inactivation of microorganisms.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132798202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1