Biophotonics South America最新文献

Estimating retinal vascular permeability using the adiabatic approximation to the tissue homogeneity model with fluorescein videoangiography 利用荧光素血管造影组织均匀性模型的绝热近似估计视网膜血管通透性

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2180643

K. Tichauer, Christian Osswald, E. Dosmar, Micah J. Guthrie, Logan Hones, L. Sinha, Xiaochun Xu, W. Mieler, K. St. Lawrence, Jennifer J. Kang-Mieler

{"title":"Estimating retinal vascular permeability using the adiabatic approximation to the tissue homogeneity model with fluorescein videoangiography","authors":"K. Tichauer, Christian Osswald, E. Dosmar, Micah J. Guthrie, Logan Hones, L. Sinha, Xiaochun Xu, W. Mieler, K. St. Lawrence, Jennifer J. Kang-Mieler","doi":"10.1117/12.2180643","DOIUrl":"https://doi.org/10.1117/12.2180643","url":null,"abstract":"Clinical symptoms of diabetic retinopathy are not detectable until damage to the retina reaches an irreversible stage, at least by today’s treatment standards. As a result, there is a push to develop new, “sub-clinical” methods of predicting the onset of diabetic retinopathy before the onset of irreversible damage. With diabetic retinopathy being associated with the accumulation of long-term mild damage to the retinal vasculature, retinal blood vessel permeability has been proposed as a key parameter for detecting preclinical stages of retinopathy. In this study, a kinetic modeling approach used to quantify vascular permeability in dynamic contrast-enhanced medical imaging was evaluated in noise simulations and then applied to retinal videoangiography data in a diabetic rat for the first time to determine the potential for this approach to be employed clinically as an early indicator of diabetic retinopathy. Experimental levels of noise were found to introduce errors of less than 15% in estimates of blood flow and extraction fraction (a marker of vascular permeability), and fitting of rat retinal fluorescein angiography data provided stable maps of both parameters.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125581964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Adapting smartphones for low-cost optical medical imaging 使智能手机适应低成本光学医学成像

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2180771

S. Pratavieira, J. D. Vollet-Filho, F. Carbinatto, K. Blanco, N. Inada, V. Bagnato, C. Kurachi

{"title":"Adapting smartphones for low-cost optical medical imaging","authors":"S. Pratavieira, J. D. Vollet-Filho, F. Carbinatto, K. Blanco, N. Inada, V. Bagnato, C. Kurachi","doi":"10.1117/12.2180771","DOIUrl":"https://doi.org/10.1117/12.2180771","url":null,"abstract":"Optical images have been used in several medical situations to improve diagnosis of lesions or to monitor treatments. However, most systems employ expensive scientific (CCD or CMOS) cameras and need computers to display and save the images, usually resulting in a high final cost for the system. Additionally, this sort of apparatus operation usually becomes more complex, requiring more and more specialized technical knowledge from the operator. Currently, the number of people using smartphone-like devices with built-in high quality cameras is increasing, which might allow using such devices as an efficient, lower cost, portable imaging system for medical applications. Thus, we aim to develop methods of adaptation of those devices to optical medical imaging techniques, such as fluorescence. Particularly, smartphones covers were adapted to connect a smartphone-like device to widefield fluorescence imaging systems. These systems were used to detect lesions in different tissues, such as cervix and mouth/throat mucosa, and to monitor ALA-induced protoporphyrin-IX formation for photodynamic treatment of Cervical Intraepithelial Neoplasia. This approach may contribute significantly to low-cost, portable and simple clinical optical imaging collection.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128633574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Comparative clinical study using laser and LED-therapy for orofacial pain relief: dentin hypersensitivity and cervicogenic headache 激光与led治疗牙本质过敏与颈源性头痛的比较临床研究

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2181117

R. Lizarelli, R. C. D. A. Pizzo, F. Florez, C. Grecco, J. Speciali, V. Bagnato

引用次数: 3

Methylene blue photodynamic therapy in rats' wound healing: 21 days follow-up 亚甲蓝光动力疗法对大鼠伤口愈合的影响:21天随访

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2181124

V. Carneiro, M. V. Catão, R. F. Menezes, N. Araújo, M. Gerbi

{"title":"Methylene blue photodynamic therapy in rats' wound healing: 21 days follow-up","authors":"V. Carneiro, M. V. Catão, R. F. Menezes, N. Araújo, M. Gerbi","doi":"10.1117/12.2181124","DOIUrl":"https://doi.org/10.1117/12.2181124","url":null,"abstract":"The experimental evaluated the photodynamic therapy (PDT) in wound healing. It used 60 male rats, making two circular wounds at each animal. They were treated at 48hs intervals, with methylene blue (MB), low level laser treatment (LLLT) or both, thus resulting in PDT. The wounds were observed 01, 03, 07, 14 and 21 days after and then processed and subjected to HE staining to analyze granulation tissue, necrosis, epithelialization and collagen. After day 1, wounds treated with MB showed necrosis less intense than other groups, and the PDT group showed more intense granulation tissue. At day 3, reepithelialization was absent for half of injuries in the PDT group, and this group was also with lower collagen. However, at day 7, this same group presented reepithelialization more advanced than control group, which did not happen with those treated with MB or LLLT (p = 0.015). The results allow us to conclude that PDT difficulted reepithelization at 7th day and interfered in standard healing. However, when used separately, MB and LLLT interfered significantly compared to the control group, which did not happened to the PDT group. There was no significant difference between the treatment groups in other analysed times.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130518268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Evaluation of cotton-fabric bleaching using hydrogen peroxide and Blue LED 双氧水和蓝光LED对棉织物漂白效果的评价

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2181026

B. P. de Oliveira, L. Moriyama, V. Bagnato

{"title":"Evaluation of cotton-fabric bleaching using hydrogen peroxide and Blue LED","authors":"B. P. de Oliveira, L. Moriyama, V. Bagnato","doi":"10.1117/12.2181026","DOIUrl":"https://doi.org/10.1117/12.2181026","url":null,"abstract":"The raw cotton production requires multiple steps being one of them the removal of impurities acquired during previous processes. This procedure is widely used by textile industries around the world and is called bleaching. The raw cotton is composed by cellulosic and non-cellulosic materials like waxes, pectins and oils, which are responsible for its characteristic yellowish color. The bleaching process aims to remove the non-cellulosic materials concentration in the fabric, increasing its whiteness degree. The most used bleaching method utilizes a bath in an alkali solution of hydrogen peroxide, stabilizers and buffer solutions under high temperature. In the present study we evaluated the possibility of using a blue illumination for the bleaching process. We used blue LEDs (450 nm) to illuminate an acid hydrogen peroxide solution at room temperature. The samples treated by this method were compared with the conventional bleaching process through a colorimetric analysis and by a multiple comparison visual inspection by volunteers. The samples were also studied by a tensile test in order to verify the integrity of the cloth after bleaching. The results of fabric visual inspection and colorimetric analysis showed a small advantage for the sample treated by the standard method. The tensile test showed an increasing on the yield strength of the cloth after blue light bleaching. The presented method has great applicability potential due to the similar results compared to the standard method, with relative low cost and reduced production of chemical waste.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134009394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of caries progression on dentin after irradiation with Nd:YAG laser by FTIR spectroscopy and fluorescence imaging Nd:YAG激光照射后牙本质龋齿进展的FTIR光谱和荧光成像研究

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2181080

P. Ana, A. Brito, D. Zezell, E. Lins

{"title":"Characterization of caries progression on dentin after irradiation with Nd:YAG laser by FTIR spectroscopy and fluorescence imaging","authors":"P. Ana, A. Brito, D. Zezell, E. Lins","doi":"10.1117/12.2181080","DOIUrl":"https://doi.org/10.1117/12.2181080","url":null,"abstract":"Considering the use of high intensity lasers for preventing dental caries, this blind in vitro study evaluated the compositional and fluorescence effects promoted by Nd:YAG laser (λ=1064 nm) when applied for prevention of progression of dentin caries, in association or not with topical application of acidulated phosphate fluoride (APF). Sixty bovine root dentin slabs were prepared and demineralized by 32h in order to create early caries lesions. After, the slabs were distributed into six experimental groups: G1- untreated and not submitted to a pH-cycling model; G2- untreated and submitted to a pH-cycling model; G3- acidulated phosphate fluoride application (APF); G4- Nd:YAG irradiation (84.9 J/cm2, 60 mJ/pulse); G5- treated with Nd:YAG+APF; G6- treated with APF+Nd:YAG. After treatments, the samples of groups G2 to G6 were submitted to a 4-day pH-cycling model in order to simulate the progression of early caries lesions. All samples were characterized by the micro-attenuated total reflection technique of Fourier transformed infrared spectroscopy (μATR-FTIR), using a diamond crystal, and by a fluorescence imaging system (FIS), in which it was used an illuminating system at λ= 405±30 nm. Demineralization promoted reduction in carbonate and phosphate contents, exposing the organic matter; as well, it was observed a significant reduction of fluorescence intensity. Nd:YAG laser promoted additional chemical changes, and increased the fluorescence intensity even with the development of caries lesions. It was concluded that the compositional changes promoted by Nd:YAG, when associated to APF, are responsible for the reduction of demineralization progression observed on root dentin.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116169309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confocal Raman study of aging process in diabetes mellitus human voluntaries 糖尿病自愿者衰老过程的共聚焦拉曼研究

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2181040

L. Pereira, C. A. Téllez Soto, L. dos Santos, S. M. Ali, P. Fávero, A. Martin

{"title":"Confocal Raman study of aging process in diabetes mellitus human voluntaries","authors":"L. Pereira, C. A. Téllez Soto, L. dos Santos, S. M. Ali, P. Fávero, A. Martin","doi":"10.1117/12.2181040","DOIUrl":"https://doi.org/10.1117/12.2181040","url":null,"abstract":"Accumulation of AGEs [Advanced Glycation End – products] occurs slowly during the human aging process. However, its formation is accelerated in the presence of diabetes mellitus. In this paper, we perform a noninvasive analysis of glycation effect on human skin by in vivo confocal Raman spectroscopy. This technique uses a laser of 785 nm as excitation source and, by the inelastic scattering of light, it is possible to obtain information about the biochemical composition of the skin. Our aim in this work was to characterize the aging process resulting from the glycation process in a group of 10 Health Elderly Women (HEW) and 10 Diabetic Elderly Women (DEW). The Raman data were collected from the dermis at a depth of 70-130 microns. Through the theory of functional density (DFT) the bands positions of hydroxyproline, proline and AGEs (pentosidine and glucosepane) were calculated by using Gaussian 0.9 software. A molecular interpretation of changes in type I collagen was performed by the changes in the vibrational modes of the proline (P) and hydroxyproline (HP). The data analysis shows that the aging effects caused by glycation of proteins degrades type I collagen differently and leads to accelerated aging process.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121935024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Oil-based gel phantom for ultrasound and optical imaging 用于超声和光学成像的油基凝胶模体

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2180642

Luciana C. Cabrelli, P. Pelissari, L. P. Aggarwal, A. Deana, A. Carneiro, T. Pavan

{"title":"Oil-based gel phantom for ultrasound and optical imaging","authors":"Luciana C. Cabrelli, P. Pelissari, L. P. Aggarwal, A. Deana, A. Carneiro, T. Pavan","doi":"10.1117/12.2180642","DOIUrl":"https://doi.org/10.1117/12.2180642","url":null,"abstract":"Water-based materials are commonly used in phantoms for ultrasound and optical imaging techniques. However, these materials have disadvantages such as easy degradation and low temporal stability. In this study, we propose an oil-based new tissue mimicking material for ultrasound and optical imaging, with the advantage of presenting low temporal degradation. Styrene-Ethylene/Butylene-Styrene (SEBS) copolymer in mineral oil samples were made varying the SEBS concentration between 5─15%, and low-density polyethylene (LDPE) between 0−9%. Acoustic properties such as speed of sound and attenuation coefficient were obtained by the substitution technique with frequencies ranging from 2.25─10 MHz, and were consistent to that of soft tissue. These properties were controlled varying SEBS and LDPE concentration; speed of sound from 1445─1480 m/s, and attenuation from 0.86─11.31 dB/cm were observed. SEBS gels with 0% of LDPE were optically transparent, presenting low optical absorption and scattering coefficients in the visible region of the spectrum. In order to fully characterize the optical properties of the samples, the reflectances of the surfaces were measured, along with the absorption. Scattering and absorption coefficients ranging from 400 nm to 1200 nm were calculated for each compound. The results showed that the presence of LDPE increased absorption and scattering of the phantoms. The results suggest the copolymer gels are promising for ultrasound and optical imaging, what make them also potentially useful for photoacoustic imaging.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132371878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Biochemical changes in cutaneous squamous cell carcinoma submitted to PDT using ATR-FTIR spectroscopy 应用ATR-FTIR光谱分析PDT后皮肤鳞状细胞癌的生化变化

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2181067

Cassio Lima, Viviane P. Goulart, P. A. D. de Castro, L. Corrêa, C. Benetti, D. Zezell

{"title":"Biochemical changes in cutaneous squamous cell carcinoma submitted to PDT using ATR-FTIR spectroscopy","authors":"Cassio Lima, Viviane P. Goulart, P. A. D. de Castro, L. Corrêa, C. Benetti, D. Zezell","doi":"10.1117/12.2181067","DOIUrl":"https://doi.org/10.1117/12.2181067","url":null,"abstract":"Nonmelanoma skin cancers are the most common form of malignancy in humans. Between the traditional treatment ways, the photodynamic therapy (PDT) is a promising alternative which is minimally invasive and do not requires surgical intervention or exposure to ionizing radiation. The understanding of the cascade of effects playing role in PDT is not fully understood, so that define and understand the biochemical events caused by photodynamic effect will hopefully result in designing better PDT protocols. In this study we investigated the potential of the FTIR spectroscopy to assess the biochemical changes caused by photodynamic therapy after 10 and 20 days of treatment using 5-aminolevulinic acid (ALA) as precursor of the photosensitizer photoporphyrin IX (PpIX). The amplitude values of second derivative from vibrational modes obtained with FTIR spectroscopy showed similar behavior with the morphological features observed in histopathological analysis, which showed active lesions even 20 days after PDT. Thus, the technique has the potential to be used to complement the investigation of the main biochemical changes that photodynamic therapy promotes in tissue.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126466494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Preparation of HIV monoclonal antibody-conjugated pulchellin in order to study its intracellular trafficking pathway in HIV-infected cells by confocal microscopy 制备HIV单克隆抗体偶联的pulchellin，用共聚焦显微镜研究其在HIV感染细胞中的细胞内转运途径

Biophotonics South America Pub Date : 2015-06-19 DOI: 10.1117/12.2181011

Mohammad Sadraeian, F. M. Tsutae, H. H. Moreira, A. P. Araujo, F. G. Guimarães, S. Pincus

{"title":"Preparation of HIV monoclonal antibody-conjugated pulchellin in order to study its intracellular trafficking pathway in HIV-infected cells by confocal microscopy","authors":"Mohammad Sadraeian, F. M. Tsutae, H. H. Moreira, A. P. Araujo, F. G. Guimarães, S. Pincus","doi":"10.1117/12.2181011","DOIUrl":"https://doi.org/10.1117/12.2181011","url":null,"abstract":"Pulchellin is a type 2 of ribosome-inactivating proteins isolated from some seeds significantly growing in Brazil. It is a potent agent to inhibit the protein synthesis in cancer cells and also HIV-infected cells. Pulchellin can be conjugated to HIV monoclonal antibodies to specifically target the HIV-infected cells. To analyze the protein synthesis inhibition by Pulchellin, the intracellular localization of the immunoconjugate should be compared to Pulchellin. In this case, the intracellular trafficking of this protein in cells can be determined by confocal microscopy. In our study, we utilized Pulchellin to construct HIV monoclonal antibody-conjugated Pulchellin A chain in order to target HIV-infected lymphocyte cells. Afterward the conjugation was labeled with the superior Alexa Fluor 488 dye. As a subsequent step, we are interested in studying the intracellular trafficking pathway of this novel conjugation in HIV-infected cells by confocal microscopy. Moreover, possible quantitative methods for fluorescent labeling of the immunoconjugate during confocal microscopy will be investigated.","PeriodicalId":307847,"journal":{"name":"Biophotonics South America","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121409148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0