Research in Molecular Medicine最新文献_第6页

Prediction of MicroRNAs bind to Toll-like Receptors Pathway in Chicken based on Bioinformatics Method 基于生物信息学方法的鸡toll样受体途径microrna结合预测

Research in Molecular Medicine Pub Date : 2019-11-10 DOI: 10.32598/rmm.7.4.33

Maryam Gholizade, J. Fayazi, S. Rahimnahal

引用次数: 2

Site-Specific PEGylation of Recombinant Immunotoxin DAB389IL-2: Structural and Functional Assessment 重组免疫毒素DAB389IL-2位点特异性聚乙二醇化:结构和功能评价

Research in Molecular Medicine Pub Date : 2019-11-10 DOI: 10.32598/RMM.7.4.7

Nasrin Zarkar, Mohammad Ali Nasiri Khalili, S. Khodadadi, M. Zeinoddini, Maryam Ghodrati Siahmazgi, N. Faramarzi

{"title":"Site-Specific PEGylation of Recombinant Immunotoxin DAB389IL-2: Structural and Functional Assessment","authors":"Nasrin Zarkar, Mohammad Ali Nasiri Khalili, S. Khodadadi, M. Zeinoddini, Maryam Ghodrati Siahmazgi, N. Faramarzi","doi":"10.32598/RMM.7.4.7","DOIUrl":"https://doi.org/10.32598/RMM.7.4.7","url":null,"abstract":"BackgroundDAB389IL-2 is considered a fusion immunotoxin and it is used for the CTCL therapy. DAB389IL-2 includes of two distinct portions; the catalytic domain of diphtheria toxin and IL-2. DAB389IL-2 duo to the presence of a free cysteine residue (Cys 513 in IL-2 part) is prone to unwanted intramolecular and intermolecular disulfide bonds formation and aggregation problems. Aggregation is considered as the most common physical instability. PEGylation is an effective approach to increase the stability and half-life of therapeutic proteins.Materials and methodsIn this study, the PEGylation of recombinant DAB389IL-2 was performed by mPEG-vinylsulfone, through partial denaturation condition at 4 0C for 24 h. To confirm the PEGylation reaction, SDS-PAGE and Dynamic Light Scattering (DLS) was used. The structure of DAB389IL-2 and PEGylated immunotoxin DAB389IL-2 was analyzed using the circular dichroism (CD) and fluorescence methods. Also, K562 cells line were treated with various concentrations of DAB389IL-2 and conjugated form. In the following, the nuclease activity of DAB389IL-2 and PEGylated form was determined.ResultsThe SDS-PAGE result confirmed the site-specific PEGylation of DAB389IL-2. Spectroscopy results exhibited that the PEGylation doesn’t affect the protein native structure. Also, cytotoxicity assay and nuclease activity test confirmed that PEGylated protein induces death in K562 cells line and DNA degradation respectively. ConclusionIt is concluded that the PEGylated immunotoxin DAB389IL-2 has a proper structure and function; thus, PEGylated immunotoxin requires more survey due its unique properties.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":"7 1","pages":"7-16"},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46460565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Chitinase3like-1 (Chiti3L1) Gene Polymorphism (rs4950928) with Susceptibility to Allergic Asthma in Iranian Northwestern Azeri Population 伊朗西北阿塞拜疆人群变应性哮喘易感性的几丁质酶3like-1 (Chiti3L1)基因多态性(rs4950928)研究

Research in Molecular Medicine Pub Date : 2019-11-10 DOI: 10.32598/RMM.7.4.17

D. E. A. Komi, M. Sadeghi-shabestari, Dariush Shanebandi, Z. Babaloo, A. Razavi, S. Sadigh-Eteghad, Tohid Kazemi

引用次数: 4

Acute Beetroot Juice Intake: Hematological, Antioxidant and Lipid Parameters in Female Athletes 急性甜菜根汁摄入:女性运动员血液、抗氧化和脂质参数

Research in Molecular Medicine Pub Date : 2019-09-30 DOI: 10.18502/rmm.v7i1.5259

M. Lotfi, M. Azizi, W. Tahmasebi, P. Bashiri

引用次数: 3

Aqueous Cannabis Extract Prevents Ethylene Glycol-induced Renal Calcium Crystallization 水大麻提取物防止乙二醇诱导肾钙结晶

Research in Molecular Medicine Pub Date : 2019-09-30 DOI: 10.18502/rmm.v7i1.5255

A. Amouzeshi, S. Z. Moossavi, Seyed Yoosef Javad Moosavi, M. Zardast, M. Malekaneh, S. Esmaili, Abdolghader Taneh, Nasim Lotfi, M. Moossavi, R. Hoshyar

引用次数: 0

Basil and Dracocephalum kotschyi Alcoholic Extracts Affect BCL2 expression and HepG2 Cell Proliferation 罗勒和龙脑醇提物对BCL2表达和HepG2细胞增殖的影响

Research in Molecular Medicine Pub Date : 2019-09-30 DOI: 10.18502/RMM.V7I1.5257

M. Moghimi, S. Rashidian, Farinaz Khosravian, Nasrin Hadi

引用次数: 2

Optimization of Cloning Conditions for High-level Production of Recombinant Mouse Interleukin-2 in Escherichia coli 重组小鼠白细胞介素-2在大肠杆菌中高产的克隆条件优化

Research in Molecular Medicine Pub Date : 2019-09-30 DOI: 10.18502/RMM.V7I1.5256

A. Abdi, Mitra Hosseinpour, Kazem Mashayekhi, M. Mousavi, Seyedeh Elham Badiee Kheirabadi, M. Sankian

引用次数: 5

Investigation of the Impact of Foretinib on AURKA and AURKB Expression in T98 Glioblastoma Cell Line 福替尼对T98胶质母细胞瘤细胞AURKA和AURKB表达影响的研究

Research in Molecular Medicine Pub Date : 2019-09-30 DOI: 10.18502/rmm.v7i1.5254

M. Moghimi, H. Sadeghi Tafti, Faezeh Namazi, Mansoor Salehi

引用次数: 1

The Evaluation of RHOXF1 Gene Expression in Different Cell Lines RHOXF1基因在不同细胞系中的表达评价

Research in Molecular Medicine Pub Date : 2019-08-10 DOI: 10.32598/RMM.7.3.9

Shadi Madipour, Shahin Amiri, M. Modarressi

引用次数: 1

Effect of Platelet-rich Plasma and Mesenchymal Stem Cells as Two Biological Alternatives in Rotator Cuff Injury Treatment: A Mini-review 富含血小板的血浆和间充质干细胞作为两种生物替代物在旋转袖带损伤治疗中的作用：综述

Research in Molecular Medicine Pub Date : 2019-08-10 DOI: 10.32598/RMM.7.3.1

Mohsen Sheykhhasan, Hamed Manoochehri, Pouria Samadi, R. Fotouhi-Ardakani, N. Kalhor

{"title":"Effect of Platelet-rich Plasma and Mesenchymal Stem Cells as Two Biological Alternatives in Rotator Cuff Injury Treatment: A Mini-review","authors":"Mohsen Sheykhhasan, Hamed Manoochehri, Pouria Samadi, R. Fotouhi-Ardakani, N. Kalhor","doi":"10.32598/RMM.7.3.1","DOIUrl":"https://doi.org/10.32598/RMM.7.3.1","url":null,"abstract":"The rotator cuff injury introduces as significant damage of trauma and others factors, and currently recognized as a prevalent orthopedic problem. Thus, many clinical procedures have been demonstrated to reduce the complication of rotator cuff injury with varying degrees of success. Platelet-Rich Plasma (PRP) is being enchantingly agreed as one of the most effectiveness strategies for the symptomatic treatment of rotator cuff injury. Many confusing parameters can influence the result of PRP including size of rotator cuff injury, patient age, PRP producing strategy, and number and timing of PRP injections. However, there is some evidences which support beneficial effect PRP in term relieving pain and restoring function, along with minimal adverse effect, in compared with corticosteroids, and other nonsurgical methods. Besides platelet-rich plasma, Mesenchymal Stem Cells (MSCs) are another biological alternative that can be used to treat rotator cuff injuries. Due to lack of large clinical trials applicability, these biological cells have challenges fairly similar to PRP. In conclusion, there is a need for further research to understand the potential application of both of these alternatives as a safe and effective therapeutic option for rotator cuff injuries. A B S T R A C T","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49259352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1