Research in Molecular Medicine最新文献

{"title":"The speed of internationalization in regionally clustered family firms: a deeper understanding of innovation activities and cluster affiliation.","authors":"Telma Mendes, Vítor Braga, Carina Silva, Alexandra Braga","doi":"10.1007/s10037-023-00182-9","DOIUrl":"10.1007/s10037-023-00182-9","url":null,"abstract":"<p><p>This article aims to explore how innovation activities and cluster affiliation moderate the relationship between family involvement and post-internationalization speed in family firms. Based on a sample of 639 Portuguese family businesses (FBs) created and internationalized between 2010 and 2018, we show that, all things being equal, higher levels of family involvement in ownership and management lead to a lower post-internationalization speed. When considering the effect of cluster affiliation, we found that clustered FBs have a lower propensity to slow down the post-internationalization process than their non-clustered counterparts. Likewise, when we account for the interactive effect of innovation activities, the results confirm that innovative FBs are less likely to slow down the post-internationalization process compared to non-innovative FBs. In addition, the concern of family firms in developing innovation was particularly pronounced when they belong to clusters. This finding is explained by the existence of socially proximate relationships with other cluster members, based on similarity, trust, knowledge exchange, and sense of belonging, which push family firms to innovate to counteract the detrimental effects of a higher family involvement in the post-internationalization speed. Our study, therefore, stresses the importance of clusters and innovation activities in moderating the relationship between family involvement and post-internationalization speed.</p><p><strong>Supplementary information: </strong>The online version of this article (10.1007/s10037-023-00182-9) contains supplementary material, which is available to authorized users.</p>","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":"2 1","pages":"1-58"},"PeriodicalIF":1.5,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89800546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upcoming Multi-drug-Resistant and Extensively Drug-Resistant Bacteria","authors":"C. Ghazaei","doi":"10.32598/rmm.10.2.820.7","DOIUrl":"https://doi.org/10.32598/rmm.10.2.820.7","url":null,"abstract":"Multi-drug-resistant (MDR) and extensively drug-resistant (XDR) bacteria are becoming a serious global health issue, which may soon become untreatable by clinicians. Since the invention of antibiotics, inappropriate consumption, non-prescribed drugs, overuse, and hoarding have caused the rapid emergence of MDR and XDR bacteria. The ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Enterobacter spp.) cause many nosocomial infections and thus escape the biocidal action of the antibiotic. Gram-positive and Gram-negative bacteria have acquired self-defense tools like ESBL (extended spectrum beta-lactamase), a mutation in porin genes, biofilm production, and many more to develop multi-drug resistance. Antimicrobial resistance (AMR) endangers patients' treatment as it causes high mortality and morbidity rates, economic loss of both patient and country, and prolonged hospital stay. To combat upcoming MDR and XDR bacteria, it is essential to design novel therapeutic techniques to eradicate such resistant bacteria via burgeoning technologies like nanoparticles, CRISPER-Cas9, genetic engineering, and synthetic biology.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43168853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization for Rapid Detection of E. coli O157:H7 Using Real-Time Loop-Mediated Isothermal Amplification","authors":"Alaleh Valiallahi, M. Zeinoddini, S. Jalili","doi":"10.32598/rmm.10.2.903.8","DOIUrl":"https://doi.org/10.32598/rmm.10.2.903.8","url":null,"abstract":"Background: E. coli O157:H7-related food poisoning is one of the most well-known causes of bloody diarrhea illness around the world. Therefore, devising a rapid, highly sensitive, and convenient detection technique for this species is crucial. In this work, we optimized a colorimetric Loop-mediated isothermal amplification (LAMP) for detecting the intimin gene from E. coli O157:H7. Methods: In this study, eae (intimin), one of the virulence factors of E. coli O157:H7, was selected as the target gene, and specific primers were designed for the sequence of this gene using the Primer Explorer V5 software. The LAMP reaction was optimized with three variable factors of MgSO4 concentration, temperature, and incubation time, in a traditional (separate) way and by Taguchi experimental design. Finally, the LAMP products were visualized by 2% agarose gel electrophoresis stained with ethidium bromide or green fluorescence (SYBR green I) and the pink fluorescence (KBC power load), which can be observed using the naked eye. Results: The LAMP reaction was optimized at 63°C and 8 mM MgSO4 for 40 min. Also, the naked eye can readily visualize the LAMP products within 40 minutes and have a detection limit of 3.2×104 CFU/mL according to 0.38 fg from the genome. Designed primers based on the gene sequence proved their specificity by testing 4 species of other common foodborne pathogenic microorganisms. Conclusion: The rapid, sensitive, one-step-visually developed LAMP assay could be of interest for screening functions in food analytical laboratories without special equipment or trained personnel.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48071233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Seroprevalence of Hydatidosis in High-risk Individuals in Sistan and Baluchestan Province, Southeast of Iran","authors":"Davood Anvari","doi":"10.32598/rmm.10.1.1225.2","DOIUrl":"https://doi.org/10.32598/rmm.10.1.1225.2","url":null,"abstract":"Background: Hydatidosis is known as one of the most prevalent zoonotic diseases across the world. This complication is also endemic in Iran, followed by a higher risk of infection in rural areas. To our knowledge, there has been no study on the seroprevalence of hydatidosis in Sistan and Baluchistan Province, Southeast of Iran. The main objective of the current study was to examine the seroprevalence of hydatidosis and its risk factors in high-risk individuals (farmers and ranchers) living in Sistan and Baluchistan Province. Materials and Methods: This study included 500 serum samples, and the participants were requested to complete a researcher-made questionnaire. Subsequently, counter-current immunoelectrophoresis (CCIEP) and enzyme-linked immunosorbent assay (ELISA) methods were employed to analyze the anti-Echinococcus granulosus antibody. The analysis of the obtained data was conducted by logistic regression in SPSS software, version 22. Results: According to the results, four (0.8%) cases were found positive for anti-E. granulosus antibody by both CCIEP and ELISA tests. Seroprevalence of hydatidosis was more in rural people, compared to those in urban areas. It was also higher in illiterate people than in educated people. Nevertheless, seropositivity showed no significant differences with age, gender, occupational status, education level, place of residence, and contact with dogs (P>0.05). Conclusion: The prevalence rate of hydatidosis in Sistan and Baluchistan Province was similar to that in neighboring provinces. According to the findings, high-risk individuals offer remarkable information about the epidemiology of hydatidosis in Sistan and Baluchistan province in southeastern Iran. This could help to manage and prevent this infection.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42091529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem Cells","authors":"Snwr Sabir Mohammad, M. Maleki","doi":"10.32598/rmm.10.2.1254.2","DOIUrl":"https://doi.org/10.32598/rmm.10.2.1254.2","url":null,"abstract":"Background: One of the most prevalent malignancies to strike women, both in Iran and globally, is cervical cancer. Metastasis, which is a significant cause of mortality, is one of the most significant pathological processes of this cancer. Therefore, preventing the migration of cancer cells may be a useful therapeutic approach. The aim of this work was to investigate the impact of conditioned media and human Wharton's jelly stem cells (hWJSCs) on the migration and growth of the cervical cancer cell line Hela as well as the in vitro mRNA expression of genes involved in metastasis. Methods: After primary culture, cellular extract and conditioned medium of hWJSCs were prepared. The viability of cervical cancer cells was investigated by MTT assay after treatment with cellular extract and conditioned medium of hWJSCs. Moreover, the anti-migratory effects of cellular extract and conditioned medium of hWJSCs on the cervical cancer cells were evaluated by wound‐healing migration assay. Finally, the mRNA expression of migration-related genes (E-cadherin and Vimentin) was detected by real-time PCR. Results: Our results indicated that the cellular extract and conditioned medium of hWJSCs (with 32% concentration) inhibited the proliferation of 100% and 20% of Hela cancer cells, respectively. In addition, the cellular extract and conditioned medium of hWJSCs significantly decreased morphological alteration and migration of the cancer cells. The cellular extract and conditioned medium of hWJSCs modified the expression of Vimentin and E-cadherin genes to inhibit the cancer cell migration (p<0.05). However, the cellular extract indicated significantly profound inhibitory effects on the cervical cancer cells compared to the conditioned medium. Conclusions: Our study demonstrated that the cellular extract and conditioned medium of hWJSCs inhibit the proliferation and migration of cervical cancer cells through the modification of migration-related gene expression. However, further in vitro and in vivo studies are required for more accurate results.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43965045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Culture Condition for the Production of Menaquinone-7 by Bacillus Subtilis Natto","authors":"M. Sharifzadeh, Negar Mottaghi-Dastjerdi, Mohammad Soltany-Rezaee-Rad, Marjan Shariatpanahi, Israa Khalid AL-Yasari, Sara Noroozi Eshlaghi","doi":"10.32598/rmm.10.1.1239.1","DOIUrl":"https://doi.org/10.32598/rmm.10.1.1239.1","url":null,"abstract":"Background: Vitamin K2 refers to a series of naphthoquinone derivatives, which have a variety of physiological and pharmacological functions for the human body. The most important type of vitamin K2 is menaquinone-7 (MK-7), an expensive raw material with no local manufacturers in Iran. Objectives: Since there was no report on the yield of MK-7 produced by the currently available Bacillus subtilis natto species in Iran, this study aims to optimize the culture condition for the production of MK-7 using this Bacillus species. Materials and Methods: The base medium (BM) for MK-7 production contained glycerol (6.3%), soybean peptone (3%), and yeast extract (0.51%). The selected factors for optimizing the MK-7 production included the incubation temperature (30, 37, and 40°C) and incubation time (72, 96, and 120hr) with/without the addition of K2HPO4 to the fermentation medium. Three sets of experiments with six modes in each set were designed based on these parameters. MK-7 content was analyzed by the HPLC method. Results: Two experiments showed the highest MK-7 production yields of 0.319 and 0.3158 mg/L. The culture condition for both of these yields was as follows:120 hours incubation time in the presence of K2HPO4. However, the incubation temperature was different in these two experiments. The incubation temperature of 30°C resulted in 0.319 mg/L MK-7 concentration, and 37°C yielded 0.3158 mg/L. Conclusion: B. subtilis natto (IBRC-M 11153) is suitable to be used as a basic platform for the mutation and production of a high-producer species. Optimizing the culture conditions using the wild-type species is not beneficial in increasing the production ability of the bacterium. It is necessary to use different methods for enhancing the production yield of MK-7 to lower the cost of microbial production and make the industrial process economic","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48887334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Frequency of Human Papillomaviruses and Epstein-Barr Virus in Colorectal Cancer Samples in Algeria","authors":"Dahmani Bouchra, Boublenza Lamia, Behar Dalale, Belatbi Kacem, Breik Ikram, Benfoula Amel, C. Nafissa, Hassaine Hafida","doi":"10.32598/rmm.10.1.1236.1","DOIUrl":"https://doi.org/10.32598/rmm.10.1.1236.1","url":null,"abstract":"Background: Environmental factors may play a role in colon cancer. In this view, several studies investigated tumor samples for the presence of various viral DNA with conflicting results. The purpose of this study is to investigate the prevalence of Human papillomaviruses (HPVs) and Epstein-Barr virus (EBV) in patients with colorectal carcinomas. Materials and Methods: In this study, we collected 74 tumorous paraffin-embedded tissues (Mean±SD age: 66.3±14.98) from the Pathology Department of a hospital in AinTemouchent and laboratories of pathological anatomy in western Algeria. DNA from each tissue was extracted and the presence of HPV and EBV was investigated using PCR. Results: None of our samples were HPV or EBV positive, and we failed to find an obvious correlation between EBV and HPV infections and this type of cancer. Conclusion: The results suggested that EBV and HPV infection is not common in patients with colorectal cancer in our population. However, the findings merit more investigations on a large number of cases.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45244861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Bilirubin, Hemin, Zinc Protoporphyrin, Glutathione, Curcumin, and Their Combinations as a Supplement to Support the Viability and Functionality of Pancreas and Liver Cells","authors":"D. Qujeq, Z. Abedian, Roghayeh Pourbagher, M. Aghajanpour, S. Fattahi, S. Edrissi","doi":"10.32598/rmm.10.1.609.8","DOIUrl":"https://doi.org/10.32598/rmm.10.1.609.8","url":null,"abstract":"Background: Previous experiments have shown different responses of pancreas and liver cells to the culture medium. It has been revealed that the most important step in preserving primary pancreas and liver cells is selecting the appropriate supplements to support the viability and functionality of these cells. Materials and Methods: Cultivation supplements were prepared by adding bilirubin, hemin, zinc protoporphyrin, glutathione, curcumin, and their combination in the pancreas and liver cell culture at a concentration of 1, 3, and 5 μM. Then, the survival rate and function of the pancreas and liver cells were evaluated. The function of pancreas cells was evaluated based on producing insulin and the function of liver cells was based on liver enzymes, including transaminases. Results: We found that bilirubin, hemin, zinc protoporphyrin, curcumin, glutathione, and their combination as supplements can dose-dependently maintain pancreas and liver cells viability and functionality proven by increasing insulin secretion levels and transaminase enzyme activity. The strength of effects is displayed in the following order: bilirubin > combination of all compounds > hemin > zinc protoporphyrin > curcumin > glutathione. Conclusion: This study shows that these compounds are suitable supplements with special biochemical properties. They provide optimal supplements for the culture media of pancreas and liver cells. They may fulfill a function in the antioxidant protection of pancreas and liver cells.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44054785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing Multi‐Epitope Subunit Vaccine Candidate for Zika Virus Utilizing In silico Tools","authors":"Fatemeh Shams Moattar, A. Asadzadeh, Maryam Heydari, M. Zamani, Fatemeh Esnaashari, Fatemeh Jeldani","doi":"10.32598/rmm.10.1.1249.1","DOIUrl":"https://doi.org/10.32598/rmm.10.1.1249.1","url":null,"abstract":"Background: The arboviruses Zika virus (ZIKV) is a pathogen that threatens human health. Scientists have warned that a single mutation in the mosquito-borne ZIKV could spark another major outbreak of the disease in humans. Therefore, designing a suitable vaccine for this virus seems necessary. This study aimed to predict the protective epitopes of envelope protein from the Zika virus with bioinformatics methods for multi-epitope vaccine development. Materials and Methods: Computational studies including the identification of potential B-cell and T-cell epitopes were used. For generating a multi-epitopic vaccine construct (MEVC), selected epitopes are connected by suitable linkers. To enhance protein immunogenicity, Maltosebound protein was added to the MEVC after the prediction and refinement of the 3D structure of the designed vaccine. The binding mode of the MEVC with toll-like receptor was investigated by molecular docking technique. Finally, molecular dynamics and in silico cloning were performed for the designed vaccine. Results: This study showed that this recombinant vaccine is nontoxic, nonallergenic, and thermostable and elicits immune responses against the Zika virus. Conclusion: The computational data suggest that the MEVC has appropriate characteristics and a high-quality structure.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43034538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Identification of Trichostrongylus Species Among Small Ruminants in Mazandaran Province, Iran","authors":"Ali Bakooie Katrimi, Naser Hoghooghi-Rad, A. Mizani, A. Amouei, S. Ranjbar-bahadori, A. Eslami, Mehdi Mehralinezhad Shiadeh, B. Laktarashi, Saeid Salehi, Tooran Nayeri Chegini, Zahra Hosseininejad, A. Pourmand, M. Sharif, A. Daryani, S. Sarvi, S. Gholami","doi":"10.32598/rmm.10.1.1248.1","DOIUrl":"https://doi.org/10.32598/rmm.10.1.1248.1","url":null,"abstract":"Background: Trichostrongylus is an intestinal parasite that is highly prevalent in humans and livestock worldwide. There is limited information about the prevalence and epidemiology of Trichostrongylus species among the infected livestock in Mazandaran Province, northern Iran. This study aimed to identify Trichostrongylus spp. among small ruminants using morphometric and molecular methods. Materials and Methods: Small intestinal organs of sheep and goats, slaughtered in Mazandaran Province, were examined for infectivity with Trichostrongylus parasites. Primary species identification was conducted based on the morphological characterization of the male worms. The internal transcribed spacer (ITS) II regions of the ribosomal DNA of the worm tissues were amplified using the polymerase chain reaction (PCR) assay and then the product was subjected to sequencing. Subsequently, the PCR products of the ITS II region were subjected to digestion by HinfI and DraI restriction enzymes using the PCR-restriction fragment length polymorphism (RFLP). Results: Of 180 samples, 98 (54.44%) were confirmed positive for Trichostrongylus based on the conventional PCR. The digestion of the PCR products with HinfI and DraI facilitated the identification of three Trichostrongylus species, namely Trichostrongylus colubriformis (35%, 90.81%), Trichostrongylus axei (4%, 4.08%), and Trichostrongylus vitrinus (5%, 5.1%). Both morphometric and RFLP techniques resulted in the differentiation of the three Trichostrongylus species. Conclusion: The present study was the 1st attempt in the last 30 years for the identification of Trichostrongylus species in small ruminants in Mazandaran Province. The findings of this study can be helpful for epidemiological and ecological studies, the establishment of effective control programs, and the management of gastrointestinal parasites in Mazandaran Province.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43263283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}