Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem Cells

Research in Molecular Medicine Pub Date : 2022-05-01 DOI:10.32598/rmm.10.2.1254.2

Snwr Sabir Mohammad, M. Maleki

{"title":"Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem Cells","authors":"Snwr Sabir Mohammad, M. Maleki","doi":"10.32598/rmm.10.2.1254.2","DOIUrl":null,"url":null,"abstract":"Background: One of the most prevalent malignancies to strike women, both in Iran and globally, is cervical cancer. Metastasis, which is a significant cause of mortality, is one of the most significant pathological processes of this cancer. Therefore, preventing the migration of cancer cells may be a useful therapeutic approach. The aim of this work was to investigate the impact of conditioned media and human Wharton's jelly stem cells (hWJSCs) on the migration and growth of the cervical cancer cell line Hela as well as the in vitro mRNA expression of genes involved in metastasis. Methods: After primary culture, cellular extract and conditioned medium of hWJSCs were prepared. The viability of cervical cancer cells was investigated by MTT assay after treatment with cellular extract and conditioned medium of hWJSCs. Moreover, the anti-migratory effects of cellular extract and conditioned medium of hWJSCs on the cervical cancer cells were evaluated by wound‐healing migration assay. Finally, the mRNA expression of migration-related genes (E-cadherin and Vimentin) was detected by real-time PCR. Results: Our results indicated that the cellular extract and conditioned medium of hWJSCs (with 32% concentration) inhibited the proliferation of 100% and 20% of Hela cancer cells, respectively. In addition, the cellular extract and conditioned medium of hWJSCs significantly decreased morphological alteration and migration of the cancer cells. The cellular extract and conditioned medium of hWJSCs modified the expression of Vimentin and E-cadherin genes to inhibit the cancer cell migration (p<0.05). However, the cellular extract indicated significantly profound inhibitory effects on the cervical cancer cells compared to the conditioned medium. Conclusions: Our study demonstrated that the cellular extract and conditioned medium of hWJSCs inhibit the proliferation and migration of cervical cancer cells through the modification of migration-related gene expression. However, further in vitro and in vivo studies are required for more accurate results.","PeriodicalId":30778,"journal":{"name":"Research in Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in Molecular Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32598/rmm.10.2.1254.2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Background: One of the most prevalent malignancies to strike women, both in Iran and globally, is cervical cancer. Metastasis, which is a significant cause of mortality, is one of the most significant pathological processes of this cancer. Therefore, preventing the migration of cancer cells may be a useful therapeutic approach. The aim of this work was to investigate the impact of conditioned media and human Wharton's jelly stem cells (hWJSCs) on the migration and growth of the cervical cancer cell line Hela as well as the in vitro mRNA expression of genes involved in metastasis. Methods: After primary culture, cellular extract and conditioned medium of hWJSCs were prepared. The viability of cervical cancer cells was investigated by MTT assay after treatment with cellular extract and conditioned medium of hWJSCs. Moreover, the anti-migratory effects of cellular extract and conditioned medium of hWJSCs on the cervical cancer cells were evaluated by wound‐healing migration assay. Finally, the mRNA expression of migration-related genes (E-cadherin and Vimentin) was detected by real-time PCR. Results: Our results indicated that the cellular extract and conditioned medium of hWJSCs (with 32% concentration) inhibited the proliferation of 100% and 20% of Hela cancer cells, respectively. In addition, the cellular extract and conditioned medium of hWJSCs significantly decreased morphological alteration and migration of the cancer cells. The cellular extract and conditioned medium of hWJSCs modified the expression of Vimentin and E-cadherin genes to inhibit the cancer cell migration (p<0.05). However, the cellular extract indicated significantly profound inhibitory effects on the cervical cancer cells compared to the conditioned medium. Conclusions: Our study demonstrated that the cellular extract and conditioned medium of hWJSCs inhibit the proliferation and migration of cervical cancer cells through the modification of migration-related gene expression. However, further in vitro and in vivo studies are required for more accurate results.

查看原文本刊更多论文

人华顿水母干细胞对宫颈癌细胞迁移的抑制作用

背景:在伊朗和全球范围内，宫颈癌是妇女最常见的恶性肿瘤之一。转移是恶性肿瘤最重要的病理过程之一，是导致死亡的重要原因。因此，阻止癌细胞的迁移可能是一种有用的治疗方法。本研究旨在探讨条件培养基和人沃顿氏水母干细胞(hWJSCs)对宫颈癌细胞系Hela的迁移和生长以及转移相关基因mRNA的体外表达的影响。方法:原代培养后，制备细胞提取液和条件培养基。用细胞提取物和条件培养基处理hWJSCs后，采用MTT法观察宫颈癌细胞的活力。此外，通过伤口愈合迁移实验评估hWJSCs细胞提取物和条件培养基对宫颈癌细胞的抗迁移作用。最后，通过实时荧光定量PCR检测迁移相关基因E-cadherin和Vimentin的mRNA表达。结果:我们的研究结果表明，细胞提取物和条件培养基(浓度为32%)对Hela癌细胞的增殖抑制率分别为100%和20%。此外，细胞提取物和条件培养基显著降低了癌细胞的形态改变和迁移。细胞提取物和条件培养基可以改变Vimentin和E-cadherin基因的表达，抑制癌细胞的迁移(p<0.05)。然而，与条件培养基相比，细胞提取物对宫颈癌细胞有明显的抑制作用。结论:我们的研究表明，hWJSCs的细胞提取物和条件培养基通过改变迁移相关基因的表达来抑制宫颈癌细胞的增殖和迁移。然而，为了获得更准确的结果，还需要进一步的体外和体内研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Research in Molecular Medicine

自引率

0.00%

发文量

审稿时长

21 weeks