Virus Reviews Research最新文献_第4页

INFLUENZA VIRUS INFECTION IN PIGS 猪流感病毒感染

Virus Reviews Research Pub Date : 2013-12-29 DOI: 10.17525/VRRJOURNAL.V18I1-2.91

J. Zanella

{"title":"INFLUENZA VIRUS INFECTION IN PIGS","authors":"J. Zanella","doi":"10.17525/VRRJOURNAL.V18I1-2.91","DOIUrl":"https://doi.org/10.17525/VRRJOURNAL.V18I1-2.91","url":null,"abstract":"Influenza A virus is a zoonotic agent of great relevance to human and animal health. Swine is an important host to the dynamics and epidemiology of the infection due to its susceptibility to viruses of both the avian and mammalian virus lineages. The great genetic variability of influenza viruses is caused by two main genetic mechanisms: point mutations and gene reassortment. The latter, by letting the exchange of gene segments between two different influenza viruses infecting the same cell, allows a rapid evolution of influenza viruses and the emergence of reassortant viruses against which there is no immunity in the host (human or animal). Influenza is endemic in pigs in many countries and the emergence of new viruses has been challenging its control and diagnostics. Although infections with influenza A virus (IAV) are endemic in most of the pork producing countries throughout the world, in Brazil this pathogen has not received much attention. Since the emergence of the 2009 pandemic H1N1 influenza virus in pigs (H1N1pdm09), many outbreaks of respiratory disease were observed in Brazilian swine populations. Currently, in Brazil, H1N1, H3N2 and 2009 pandemic H1N1 (A(H1N1)pdm09) influenza A viruses (IAVs) circulate in domestic swine herds. DOI: http://dx.doi.org/10.17525/vrrjournal.v18i1-2.91","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"18 1","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2013-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Active cytomegalovirus (CMV) infection in liver recipients in a high CMV seroprevalence region - outcomes and the use of antigenemia 巨细胞病毒(CMV)在巨细胞病毒(CMV)高血清阳性率地区的肝脏受体的活动性感染——结果和抗原血症的使用

Virus Reviews Research Pub Date : 2013-12-29 DOI: 10.17525/vrrjournal.v18i1-2.89

A. Cunha, A. Cunha, D. Solla, R. Chaves, B. G. C. Filho, R. Nascimento

{"title":"Active cytomegalovirus (CMV) infection in liver recipients in a high CMV seroprevalence region - outcomes and the use of antigenemia","authors":"A. Cunha, A. Cunha, D. Solla, R. Chaves, B. G. C. Filho, R. Nascimento","doi":"10.17525/vrrjournal.v18i1-2.89","DOIUrl":"https://doi.org/10.17525/vrrjournal.v18i1-2.89","url":null,"abstract":"Cytomegalovirus (CMV) is the most frequent viral infection in liver recipients, acting as immunomodulatory factor for \u0000other opportunistic infections and rejection. We assessed the outcomes of CMV infection in liver recipients in a high \u0000CMV seroprevalence region and the use of antigenemia for the diagnosis of CMV syndrome. Between March 2007 \u0000and April 2009, 44 liver recipients collected 344 samples for CMV antigenemia. Defi nition of active CMV infections \u0000used literature criteria. Recipients’ outcomes [CMV syndrome, Hepatitis C Virus (HCV) recurrence, rejection and \u0000mortality] were analyzed. Performance of antigenemia for the diagnosis of CMV syndrome was assessed by the area \u0000under the Receiver Operating Curve (AUROC) of 52 positive samples, representing 24 recipients. CMV serology was \u0000positive (R+) in 90.9% of liver recipients. CMV syndrome occurred in 18 (40.9%) recipients. CMV negative serology \u0000(R-) recipients had lower disease-free time, as well as lower one-year and four-year survival rates (p = 0.022 and p = \u00000.004, respectively). HCV+ recipients presented CMV-associated indirect eff ects and had a tendency to lower fouryear \u0000survival rate (p=0.089). Th e AUROC for CMV syndrome was 0.745 (95% CI 0.606 to 0.856, p = 0.006), with a \u0000cut-off of more than 8 positive cells/200,000 leukocytes, (sensitivity of 88.9% and specifi city of 74.4%). CMV infection \u0000is associated to morbidity and lower survival rates in liver recipients in a high CMV seroprevalence region. Using \u0000antigenemia, the cut-off for diagnosing CMV syndrome was higher than 8 positive cells/200,000 leukocytes, with an \u0000appropriated performance through its accuracy.","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"18 1","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2013-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA INTERFERENCE AS ANTIVIRAL THERAPY: DREAM OR REALITY? RNA干扰抗病毒治疗:梦想还是现实?

Virus Reviews Research Pub Date : 2013-12-19 DOI: 10.17525/VRR.V18I1-2.92

Z. Nizamani, C. Holz, D. Keita, G. Libeau, E. Albina, R. Almeida

引用次数: 1

A BRIEF HISTORY OF White spot syndrome virus AND ITS EPIDEMIOLOGY IN BRAZIL 巴西白斑综合征病毒简史及其流行病学研究

Virus Reviews Research Pub Date : 2013-12-13 DOI: 10.17525/VRR.V18I1-2.63

R. N. Santos, A. Varela, S. Cibulski, F. E. S. Lima, F. Spilki, Ls Heinzelmann, R. Luz, P. Abreu, P. Roehe, L. Cavalli

引用次数: 7

Immunobiologicals in Virology 病毒学中的免疫生物制剂

Virus Reviews Research Pub Date : 2013-09-04 DOI: 10.17525/VRR.V18I1-2.85

Sociedade Brasileira de Virologia

引用次数: 0

Helio Gelli Pereira Award and Oral Presentation Helio Gelli Pereira奖和口头报告

Virus Reviews Research Pub Date : 2013-09-04 DOI: 10.17525/VRR.V18I1-2.81

Sociedade Brasileira de Virologia

引用次数: 0

CRY4AA AND CRY4BA FROM BACILLUS THURINGIENSIS SUBSP. ISRAELENSIS EXPRESSED IN INSECT CELLS BY RECOMBINANT BACULOVIRUSES ARE TOXIC TO AEDES AEGYPTI LARVAE 苏云金芽孢杆菌亚种Cry4aa和cry4ba。重组杆状病毒在昆虫细胞中表达的以色列蚊对埃及伊蚊幼虫具有毒性

Virus Reviews Research Pub Date : 2013-08-23 DOI: 10.17525/vrr.v18i1-2.70

R. F. Corrêa, R. S. Aguiar, R. Monnerat, B. Ribeiro

{"title":"CRY4AA AND CRY4BA FROM BACILLUS THURINGIENSIS SUBSP. ISRAELENSIS EXPRESSED IN INSECT CELLS BY RECOMBINANT BACULOVIRUSES ARE TOXIC TO AEDES AEGYPTI LARVAE","authors":"R. F. Corrêa, R. S. Aguiar, R. Monnerat, B. Ribeiro","doi":"10.17525/vrr.v18i1-2.70","DOIUrl":"https://doi.org/10.17525/vrr.v18i1-2.70","url":null,"abstract":"Bacillus thuringiensis subsp. israelensis (Bti) is highly toxic to mosquito larvae. Most of its toxicity relies on δ-endotoxins (Cry and Cyt) crystalline inclusions produced at the sporulation phase of growth. Obtention of individual Bti mosquitocidal toxins is crucial for the understanding of Bti´s potential activity against dipteran larvae. A Cry toxin expression system based on baculovirus was developed to produce two of the Cry toxins comprised in the crystals of Bti. Th e cry4Aa and cry4Ba genes from two Brazilian strains of Bacillus thuringiensis were amplified by PCR, cloned into a plasmid cloning vector and sequenced. Sequence analysis of the cry4Aa and cry4Ba genes showed high identity to previous known cry genes. Both cry4Aa and cry4Ba genes were further cloned into transfer vectors for construction of recombinant baculoviruses. After isolation of the recombinant viruses, they were used to infect insect cells (BTITN5B1-4) that were analyzed by light microscopy at different times post infection. Putative crystals consisting of either Cry4Aa or Cry4Ba were observed in the cytoplasm of infected insect cells. RT-PCR was performed with mRNA from insect cell extracts (72 h.p.i.) in order to confirm the presence of the genes specific transcripts. Recombinant virus-infected insect extracts (120 h.p.i.) were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE) showing the presence of polypeptide bands of around 128 and 130 kDa, corresponding, respectively to the sizes of the proteins Cry4Aa and Cry4Ba. Bioassays with virus-infected insect extracts were shown to be toxic to second instar Aedes aegypti larvae, confirming the usefulness of this expression system for the study of Cry proteins. DOI: http://dx.doi.org/10.17525/vrr.v18i1-2.70","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"31 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2013-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PERIANAL SQUAMOUS CELL CARCINOMA WITH HIGH-GRADE ANAL INTRAEPITHELIAL NEOPLASIA IN AN HIV-NEGATIVE PATIENT 1例hiv阴性患者伴高级别肛门上皮内瘤变的肛周鳞状细胞癌

Virus Reviews Research Pub Date : 2013-06-17 DOI: 10.17525/VRR.V18I1-2.64

E. Dobao, L. Afonso, C. Pires, B. K. Kac, L. Fonseca, W. Menezes, J. Nery, S. Cavalcanti

{"title":"PERIANAL SQUAMOUS CELL CARCINOMA WITH HIGH-GRADE ANAL INTRAEPITHELIAL NEOPLASIA IN AN HIV-NEGATIVE PATIENT","authors":"E. Dobao, L. Afonso, C. Pires, B. K. Kac, L. Fonseca, W. Menezes, J. Nery, S. Cavalcanti","doi":"10.17525/VRR.V18I1-2.64","DOIUrl":"https://doi.org/10.17525/VRR.V18I1-2.64","url":null,"abstract":"HPV infection and associated diseases in the male population has assumed importance, especially because of the increase in incidence of anal carcinoma. The recognition that man is no longer mere infection vector for this disease is still poorly discussed, and can result in diagnostic losses and further increase in anogenital disease morbidity. In this study, we aimed to present a case of an atypical anogenital lesion caused by HPV infection in a HIV(-) male patient. Clinical exam showed perianal lesion. This lesion has been misdiagnosed and treated as herpes and candidiasis for almost ten years, when ulceration was observed, followed by purulent secretion. Histopathology showed intraepithelial neoplasia constituted by atypical keratinocytes, loss of polarity and corneal pearls compatible with Bowen’s disease. Specific PCR showed HPV 16 DNA in both episomal and integrated forms. Successful treatment was achieved by radiotherapy. The knowledge about the behavior of anogenital HPV infection in men is still unclear, even for high-risk groups such as MSM (men who have sex with men) and HIV seropositive. Therefore, several patients have been maintained without diagnosis, being regarded as sources of infection and even suffering from high-grade lesions with clinical unsatisfactory outcomes. Our goal with this case report is to contribute to the dissemination of an emerging problem, stimulating discussion and the search for new tools of early recognition and treatment of male anogenital lesions. DOI: http://dx.doi.org/10.17525/vrr.v18i1-2.64","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"1 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2013-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WATERBORNE VIRAL PATHOGENS: DETECTION, CONTROL AND MONITORING OF WATER QUALITY FOR HUMAN CONSUMPTION 水媒病毒性病原体:供人类食用的水质检测、控制和监测

Virus Reviews Research Pub Date : 2013-06-10 DOI: 10.17525/VRRJOURNAL.V18I1-2.65

L. Faccin-Galhardi, Nayara Lopes, S. Espada, R. Linhares, J. Pelayo, C. Nozawa

{"title":"WATERBORNE VIRAL PATHOGENS: DETECTION, CONTROL AND MONITORING OF WATER QUALITY FOR HUMAN CONSUMPTION","authors":"L. Faccin-Galhardi, Nayara Lopes, S. Espada, R. Linhares, J. Pelayo, C. Nozawa","doi":"10.17525/VRRJOURNAL.V18I1-2.65","DOIUrl":"https://doi.org/10.17525/VRRJOURNAL.V18I1-2.65","url":null,"abstract":"The viral contamination of water for human consumption is a common cause of diseases outbreaks, therefore, a matter of great relevance for public health, especially in developing countries. In most countries, legislation recommends the study of coliforms to indicate the potability of water, however, this is not correlated with the presence of virus. The present review sought to characterize the epidemiological importance of waterborne viral diseases and the limitations of laboratorial detection and elimination of these pathogens. Besides, the state of art of virus as a tool to assess the quality of the water, as well as, the use of a single virus as the indicator of environmental contamination by virus itself are presented. Reports have demonstrated that some viruses are resistant to conventional water treatment process. Changes in water treatment policy should be achieved, and, should involve viral detection and resistance to physical and chemical agents, amongst other methods. However, we have to bear in mind that the decrease of waterborne infectious diseases is greatly dependent upon the improvement of sanitation, water treatment, together with preventive measures. All those measures together could ensure appropriate conditions for the promotion of human health and the reduction of environmental impacts. DOI: http://dx.doi.org/10.17525/vrrjournal.v18i1-2.65","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"18 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2013-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

ASSOCIATION BETWEEN GLUTATHIONE S-TRANSFERASE GENOTYPES, HUMAN PAPILLOMAVIRUS INFECTION AND DEVELOPMENT OF CERVICAL INJURY IN OLINDA -PE 谷胱甘肽s -转移酶基因型、人乳头瘤病毒感染与奥林达型颈椎损伤发生的关系

Virus Reviews Research Pub Date : 2013-04-20 DOI: 10.17525/vrrjournal.v18i1-2.66

Adriano José de Oliveira Soares, D. Martins, Adrya Lúcia Perez Bezerra de Medeiros, J. L. L. Filho, Rosângela Ferreira Frade de Araújo

{"title":"ASSOCIATION BETWEEN GLUTATHIONE S-TRANSFERASE GENOTYPES, HUMAN PAPILLOMAVIRUS INFECTION AND DEVELOPMENT OF CERVICAL INJURY IN OLINDA -PE","authors":"Adriano José de Oliveira Soares, D. Martins, Adrya Lúcia Perez Bezerra de Medeiros, J. L. L. Filho, Rosângela Ferreira Frade de Araújo","doi":"10.17525/vrrjournal.v18i1-2.66","DOIUrl":"https://doi.org/10.17525/vrrjournal.v18i1-2.66","url":null,"abstract":"Human papillomavirus (HPV) is responsible for developing cancers, among them cervical cancer, which is the second cancer responsible for female mortality. The polymorphism of glutathione S-transferase (GST) is closely linked to an increased risk of cervical lesions and HPV infection. This enzyme is involved in phase II of xenobiotic metabolism, which can eliminate potential toxic carcinogenic compounds from the body. In this study, it was analysed the association between GSTM1 and/or GSTT1 polymorphism and susceptibility to HPV infection or development of cervical lesions. It was also analysed the correlation of these polymorphisms with external factors such as number of sexual partners, parity, smoking and alcohol consumption. Women who had more than one sexual partner presented 2.35 times more chances of being infected by HPV, but it could not be associated to the development of cervical lesions. However, more than three pregnancies leads to high risk (OR=3.2) of developing cervical lesions. Considering the genotypes analyzed in this study, only GSTT1 null showed significant influence on the risk of HPV infection among consumers of alcohol (OR=6.6) and none of them showed influence on the risk of developing cervical lesions (P <0.05). The study of other polymorphisms and expression of GST isoforms and enzymes that act in phase I of xenobiotics metabolism, for example, could lead to further clarification of the effect of oxidative stress on cellular susceptibility to HPV infection, since the results this research point to this interaction. DOI: http://dx.doi.org/10.17525/vrrjournal.v18i1-2.66","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"18 1","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2013-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0