Assiut Veterinary Medical Journal最新文献_第9页

EVALUATION OF THE HEALING OF WOUNDS DRESSED WITH ZINC METAL-ORGANIC FRAMEWORKS (ZN-MOFS) IN DOGS: AN EXPERIMENTAL STUDY 评价锌金属有机框架(zn-mofs)在狗伤口愈合的实验研究

Assiut Veterinary Medical Journal Pub Date : 2022-10-01 DOI: 10.21608/avmj.2022.157557.1085

Reham M. ABD-EL AZEEM, Ahmed Ibrahim, Mohamed H. Kotob, Abdelnaby M. Elshahawy, Samia Selim

{"title":"EVALUATION OF THE HEALING OF WOUNDS DRESSED WITH ZINC METAL-ORGANIC FRAMEWORKS (ZN-MOFS) IN DOGS: AN EXPERIMENTAL STUDY","authors":"Reham M. ABD-EL AZEEM, Ahmed Ibrahim, Mohamed H. Kotob, Abdelnaby M. Elshahawy, Samia Selim","doi":"10.21608/avmj.2022.157557.1085","DOIUrl":"https://doi.org/10.21608/avmj.2022.157557.1085","url":null,"abstract":"This study aimed to evaluate the healing of wounds dressed with Zn-MoF in dogs. The study was conducted on fifteen clinically healthy mongrel dogs. Each dog has bilateral cutaneous excisional wounds (2 × 2 cm 2 ). Right-side wounds were dressed with Zn-MOF dressing (treated wounds) under the effect of 1 mg/kg of xylazine HCL 2% and 10 mg/kg of ketamine HCL 5% , administered in one syringe intramuscularly (IM), while left-side wounds were dressed with normal saline (control group). Wounds were undergone to histopathological evaluation 7-, 15-, and 21-days post-wound induction (5 dogs each interval). Zn-MOF positively enhanced the re-epithelization of the wound area promoting the epidermal hyperplasia resulting in reduction of the wound size and epithelial gap that was completely closed and restored on day 21 post-wound induction. The control wounds were at a slower healing rate with time leaving epithelial gaps and did not completely close day 21 post-wound induction. Zn-MOF treated wounds’ dermis was pervaded with the inflammatory cells on day 7 post-wound induction that gradually reduced by time and replaced by fibroblasts 14- and 21-days post-wound induction. The dermis of control wounds was severely infiltrated with a larger number of inflammatory cells and excessive hemorrhage throughout the study. Zn-MOF treated wounds had an augment in the number and size of newly formed blood vessels in comparison to the control ones, reaching their highest point on day 14- and declining on day 21 post-wound induction. Collagen deposition increased obviously 21 days post-wounding in Zn-MOF treated wounds. Zn-MOF accelerated and enhanced the wound healing process and abundant granulation tissue formation in dogs.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129339921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DETECTION OF SALMONELLA AND HELICOBACTER SPP. IN CAPTIVE WILD FELIDS 圈养野田沙门氏菌和幽门螺杆菌的检测

Assiut Veterinary Medical Journal Pub Date : 2022-10-01 DOI: 10.21608/avmj.2022.149762.1071

Ahmed M. Salah-Eldein, Noura A. Tawfik, Gamal G. Medani, A. Wahdan, N. Eidaroos

{"title":"DETECTION OF SALMONELLA AND HELICOBACTER SPP. IN CAPTIVE WILD FELIDS","authors":"Ahmed M. Salah-Eldein, Noura A. Tawfik, Gamal G. Medani, A. Wahdan, N. Eidaroos","doi":"10.21608/avmj.2022.149762.1071","DOIUrl":"https://doi.org/10.21608/avmj.2022.149762.1071","url":null,"abstract":"The current study aimed to investigate the presence of Salmonella and Helicobacter species in captive wild felids in addition to perform serotyping, antibiotic sensitivity test to the isolated Salmonella spp. and detection of antibiotic resistance and virulence genes. A total of 60 fecal samples were collected from 30 captive wild felids from Giza zoo and private zoo in Egypt. All animals were apparent healthy except eight African lions ( Panthera Leo ) have a history of vomiting. Samples were examined bacteriologically for the presence of Salmonella spp., followed by biochemical and serological tests. Moreover, obtained isolates were subjected to antimicrobial sensitivity testing and detection of antibiotic resistance and virulence genes. Fecal samples from lions with history of vomiting, were subjected to direct molecular identification for detection of Helicobacter spp. Overall, Salmonella spp. were isolated from 3 wild cats ( Felis chaus) . Two serovars of Salmonella were detected; S. Bovismorbificans and S. Southampton while Helicobacter felis was isolated from one African lion. Isolates of Salmonella spp. showed complete resistance to cefaclor (100%), cefoxitin (100%), and cefadroxil (100%); and very high resistance to tobramycin (66.7%), while it completely sensitive to Azithromycin (100%), Sulfa/trimethoprim (100%), Nitrofurantoin (100%), Doxycycline (100%), Amoxicillin-Clavulanic acid (100%), Fosfomycin (100%) and Oxytetracycline (100%). bla TEM and bla SHV were confirmed in Salmonella isolates showing resistant to Cefaclor and Cefoxitin, and aadA2 in S. Bovismorbificans that showing resistant to tobramycin. S. Southampton and S. Bovismorbificans have invA, stn, sopB , and hilA genes while S. Bovismorbificans carry also pefA gene as a virulence genes.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"136 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132083029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

ASSESSMENT OF NUTRITIVE VALUE AND HYGIENIC STATE OF LIVER (KIBDA) AND SLICED MEAT SANDWICHES IN NEW VALLEY GOVERNORATE 新谷省肝(kibda)和切片肉三明治的营养价值和卫生状况评估

Assiut Veterinary Medical Journal Pub Date : 2022-10-01 DOI: 10.21608/avmj.2022.157710.1083

Shaimaa Mohammed ABD EL-MOTELB, Sherief Mohammed SAYED ABD-ALLAH, Hesham ABDEL-MOEZ AHMED ISMAIL

{"title":"ASSESSMENT OF NUTRITIVE VALUE AND HYGIENIC STATE OF LIVER (KIBDA) AND SLICED MEAT SANDWICHES IN NEW VALLEY GOVERNORATE","authors":"Shaimaa Mohammed ABD EL-MOTELB, Sherief Mohammed SAYED ABD-ALLAH, Hesham ABDEL-MOEZ AHMED ISMAIL","doi":"10.21608/avmj.2022.157710.1083","DOIUrl":"https://doi.org/10.21608/avmj.2022.157710.1083","url":null,"abstract":"In the present work, 50 samples of each ready-to-eat (RTE) sandwiches of the liver (kibda) and sliced meat were collected at random from the points of sale in El-Kharga city, New Valley Governorate, Egypt. The hygienic (coliforms, fecal coliforms, E. coli . yeast, and mould counts) and nutritional (moisture, protein, fat, ash, gross energy, and cholesterol content) quality were assessed. All samples were sensory accepted. The coliforms were detected in 52 and 50%; fecal coliforms in 10 and 2%; and E. coli in 4 and 2% of the examined RTE sandwiches of kibda and sliced meat, respectively. Pathogenic E. coli strains were identified from the liver (3 strains) and sliced meat (1 strain) samples. The average yeast count was 4.20±0.0.25, and 3.46±0.17; while that of mould was 3.18±0.13 and 2.90±0.07 log 10 cfu/g, respectively. The average moisture contents (%) were 55.62±0.43 and 43.50±0.68; protein (%) were 24.29±0.47 and 24.45±0.60; fat (%) were 10.41±0.25 and 16.13±0.43; and ash (%) were 2.75±0.08 and 1.41±0.06, respectively. The average gross energy contents (Kcal/100g) were 190.90±3.30 and 243.0±4.6, respectively. The average total cholesterol contents (mg/100g) were 60.12±6.93 and 50.45±6.02, respectively. In conclusion, although nutritious, RTE sandwiches under investigation may pose public health concerns (pathogenic bacteria and cholesterol), especially those of liver (kibda).","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"112 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132148350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EFFECT OF HOT DRY ENVIRONMENT ON THE OXIDATIVE STRESS INDICES IN MALE BARKI LAMBS 干热环境对猕猴桃公羊羔氧化应激指标的影响

Assiut Veterinary Medical Journal Pub Date : 2022-07-01 DOI: 10.21608/avmj.2022.143473.1065

Mostafa A. Saleh, M. Rateb, E. A. A. ABD-ALLAH, G. Mohamed

{"title":"EFFECT OF HOT DRY ENVIRONMENT ON THE OXIDATIVE STRESS INDICES IN MALE BARKI LAMBS","authors":"Mostafa A. Saleh, M. Rateb, E. A. A. ABD-ALLAH, G. Mohamed","doi":"10.21608/avmj.2022.143473.1065","DOIUrl":"https://doi.org/10.21608/avmj.2022.143473.1065","url":null,"abstract":"Heat stress (HS) is the main challenge facing livestock health. The present work aimed to study the oxidative stress status in the blood of desert Barki lambs during the hot dry season in El-Kharga oasis in the western Egyptian desert. Twenty-four male Barki lambs (4-5 months) were included in this study. Twelve of them were selected during July (hot dry, HS group) and the remaining 12 animals were selected during December (thermoneutral, TN-group) as controls. Temperature humidity index (THI) registered 65.4 (satisfactory) in winter and 89.6 (risky HS) in summer . Compared to controls, increased rectal temperature ( P < 0.001) and respiration rate ( P < 0.001) were detected in the HS group. Red blood cells count ( P < 0.001), packed cell volume ( P < 0.01) and hemoglobin concentration ( P < 0.001) were reduced in HS group. Increased total peroxides (TPx , P < 0.05), the free radical superoxide anion ( P < 0.05) and decreased total antioxidant capacity (TAC, P < 0.05) were shown in plasma of the HS group. Malondialdehyde concentration as an indicator of lipid peroxidation was increased ( P < 0.05) but superoxide dismutase activity was decreased ( P < 0.05) in the erythrocyte of the HS group. Oxidative stress index (OSI, TPx/TAC ratio) increased ( P < 0.05) in HS group. In conclusion, HS is associated with oxidative stress and corpuscular redox imbalance in lambs under tropical conditions.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"121 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130346658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

USING LACTOFERRIN AS A TRAIL TO CONTROL E COLI AND STAPH. AUREUS ISOLATED FROM SOME TYPES OF CHEESE 乳铁蛋白对大肠杆菌和葡萄球菌的抑制作用。从某些奶酪中分离出的金黄色葡萄球菌

Assiut Veterinary Medical Journal Pub Date : 2022-07-01 DOI: 10.21608/avmj.2022.133363.1056

Azhar MOHAMMED HASSAN, Jakleen HALIM TAWFIK BEBAWY, Mary RAFAT HAFAZ, Walaa S. HASAN

{"title":"USING LACTOFERRIN AS A TRAIL TO CONTROL E COLI AND STAPH. AUREUS ISOLATED FROM SOME TYPES OF CHEESE","authors":"Azhar MOHAMMED HASSAN, Jakleen HALIM TAWFIK BEBAWY, Mary RAFAT HAFAZ, Walaa S. HASAN","doi":"10.21608/avmj.2022.133363.1056","DOIUrl":"https://doi.org/10.21608/avmj.2022.133363.1056","url":null,"abstract":"In this study, the effect of lactoferrin as an antibacterial activity on the phagocytic activity of E. coli as Gram-negative bacteria and Staph. aureus as Gram-positive bacteria was investigated. The microbiological status of different types of cheese (Kareish, Domiati and Tallaga cheese) was also evaluated. A total of sixty cheese samples (20 samples each) were collected randomly from different markets and restaurants. Pathogenic bacteria including E. coli and Staph aureus were isolated and considered as indicators of the microbiological quality. The effect of lactoferrin on E. coli and Staph. aureus was evaluated to improve the quality of cheese. Lactoferrin antibacterial activity was tested using different concentrations of lactoferrin (zero, 0.5, 1.0, 5.0, 10, and 20 mg/ml) on the survivability of E. coli and Staph. aureus in different varieties of cheese. Lactoferrin showed various inhibition activity on E. coli viability than Staph. aureus , and significantly influenced the count of E. coli in Kareish and Domiati cheese while, and 20% of lactoferrin can inhibit the viability of Staph. aureus in Kareish cheese. Furthermore, their viability in Tallaga cheese was not significantly affected by lactoferrin even by using high concentration. So, lactoferrin could become a promising method to decrease the viability of E. coli and Staph. aureus in cheese.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123510230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

INFLUENCE OF DIFFERENT FORMULATIONS OF ALGINATE-BASED FILMS IN THEIR ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY IN MEAT SLICES 不同配方海藻酸盐基薄膜对肉片抗菌和抗氧化活性的影响

Assiut Veterinary Medical Journal Pub Date : 2022-06-29 DOI: 10.21608/avmj.2022.143221.1064

M. Ammar, M. Mohamed

{"title":"INFLUENCE OF DIFFERENT FORMULATIONS OF ALGINATE-BASED FILMS IN THEIR ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY IN MEAT SLICES","authors":"M. Ammar, M. Mohamed","doi":"10.21608/avmj.2022.143221.1064","DOIUrl":"https://doi.org/10.21608/avmj.2022.143221.1064","url":null,"abstract":"In the current study, the antibacterial and antioxidant effects of three formulated alginate-based edible coats on refrigerated beef were investigated. The formulated coats included 2 organic acids (OAs) based (T1 and T2) and one nisin-OAs based (T3). Control and coated beef slices were sampled immediately (zero time) and then periodically for 15 days of storage at 1  C. The antibacterial effects of coating were assessed by aerobic plate count (APC), Enterobacteriaceae and coliforms count. Peroxide value (POV) and catalase (CAT) activity were applied as indexes of oxidation. Moreover, the cupper sulphate test and pH were used as freshness indexes. The coats appeared efficient antibacterials. By coating, APC didn't exceed 6 log cfu/g 15 for days of storage with OAs-based coats more significant. T1 and T2 were more effective against enteric bacteria where the load of Enterobacteriaceae and coliforms were reduced to undetectable levels for 15 days. The coats also successfully functioned as antioxidants. POV of coated slices significantly reduced where their values were 2.27, 1.87, 2.27 and 2.20 (meq/kg) for control, T1, T2 and T3, respectively after 15 days of storage. Nisin-OAs-based coats appeared the most antioxidant defense mechanism in stored beef where CAT levels were 0.005, 0.010, 0.007 and 0.044 U/g for control, T1, T2 and T3, respectively at end of 15 days of storage. According to freshness indexes, coated slices continued fresh for at least 3 days in excess to control with nisin-OAs coating the most effective. The obtained data indicate that studied coats can be effectively applied to improve the marketability of beef and preserve its","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116543407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginger oil alleviates sero-biochemical and histopathological changes in pancreatic and liver tissues of diabetic induced rats 姜油对糖尿病大鼠胰腺和肝脏组织的血清生化和病理改变有一定的缓解作用

Assiut Veterinary Medical Journal Pub Date : 2022-06-29 DOI: 10.21608/avmj.2022.141277.1062

Nermeen Mohamed, M. Abd El-Samei, sary Abd El-ghaffar, F. Ahmed

引用次数: 0

Physicochemical analysis and microbial evaluation of butter sold in Assiut City, Egypt 埃及阿西尤特市售黄油的理化分析和微生物评价

Assiut Veterinary Medical Journal Pub Date : 2022-06-19 DOI: 10.21608/avmj.2022.140392.1061

S. Hassan, AMIN, W.F., O. Sadek

{"title":"Physicochemical analysis and microbial evaluation of butter sold in Assiut City, Egypt","authors":"S. Hassan, AMIN, W.F., O. Sadek","doi":"10.21608/avmj.2022.140392.1061","DOIUrl":"https://doi.org/10.21608/avmj.2022.140392.1061","url":null,"abstract":"In this aspect, this study aimed to evaluate the quality of pasteurized and cooking (Falahy) butter in Assiut City, Egypt. A total of random 160 pasteurized and Falahy butter samples (80 samples each) were bought from different localities in Assiut city. The sensory assessment for pasteurized and Falahy butter showed that 78.75, 62.5% of samples exceeded a score of 90 (excellent) and 21.25, 37.5% had a score (80-89). The mean values of peroxide index, TBRA reactive substances and acid index were (0.39±0.02, 0.44±0.03 meq/kg), (0.46±0.03, 0.51±0.02 mg MDA/Kg) and (0.67±0.01, 0.82±0.02 mg KOH/g) for pasteurized and Falahy butter samples, respectively which revealed that all samples were acceptable with the absence of both hydrolytic and oxidative types of rancidity. The mean values of the microbiological evaluation were (1.3×10 ± 1.6×10, 8.8x10 ± 1.3×10), (1×10 ± 2.3×10, 3.2×10 ± 3.3×10), (5.7×10 ± 1.3×10, 9.4×10 ± 2×10), (1.5×10 ± 3×10, 2.8×10 ± 4.5×10) and (1.3×10 ± 3.3×10, 1.6×10±4.2×10) CFU/g for the total colony, psychrotrophic, lipolytic, yeast and mold counts in pasteurized and Falahy butter samples, respectively. The results revealed the substandard production and storage conditions which call for improvement of butter production modern technologies and awareness creation about the hygienic production, processing and handling of butter.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127386342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Molecular Identification of Virulence Genes of Pathogenic Escherichia coli Isolated from Broilers Chicken 肉鸡致病性大肠杆菌毒力基因的分子鉴定

Assiut Veterinary Medical Journal Pub Date : 2022-06-19 DOI: 10.21608/avmj.2022.132991.1054

HossamM Abdel Aziz, Mostafa shata, N. M. Ali, N. Hagag, O. Amin

引用次数: 0

MICROBIOLOGICAL EVALUATION OF LOCALLY MANUFACTURED SOFT CHEESE 本地生产软奶酪的微生物学评价

Assiut Veterinary Medical Journal Pub Date : 2022-06-19 DOI: 10.21608/avmj.2022.140401.1060

Rasha R. Eid, Ahmed Ahmed, W. Amin, M. Amin

引用次数: 2