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Simultaneous Determination of Penicillin G and Chloramphenicol in Milk by a Magnetic Nanoparticle-Based Fluorescent Immunoassay 磁性纳米粒子荧光免疫法同时测定牛奶中青霉素G和氯霉素
The Open Biotechnology Journal Pub Date : 2020-06-16 DOI: 10.2174/1874070702014010059
M. Atanasova, Y. Ivanov, E. Zvereva, A. Zherdev, T. Godjevargova
{"title":"Simultaneous Determination of Penicillin G and Chloramphenicol in Milk by a Magnetic Nanoparticle-Based Fluorescent Immunoassay","authors":"M. Atanasova, Y. Ivanov, E. Zvereva, A. Zherdev, T. Godjevargova","doi":"10.2174/1874070702014010059","DOIUrl":"https://doi.org/10.2174/1874070702014010059","url":null,"abstract":"Magnetic nanoparticles were synthesized and functionalized with (3-aminopropyl)triethoxysilane. Chloramphenicol-Ovalbumin and Chloramphenicol-Ovalbumin-Fluorescein-5-isothiocyanate conjugates were prepared. Penicillin G – ATTO 633 fluorescent conjugate was synthesized. Antibodies against chloramphenicol and penicillin G were immobilized onto the magnetic nanoparticles. The competitive fluorescent immunoassay was developed. The optimal concentration of the antibody-magnetic nanoparticles and the fluorescent conjugates for the assay was determined. The calibration curves for the antibiotics in buffer and milk were plotted. Fluorescent immunoassay for the simultaneous determination of chloramphenicol and penicillin G in milk was developed.","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115670002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Tea from the Food Science Perspective: An Overview 食品科学视角下的茶叶:综述
The Open Biotechnology Journal Pub Date : 2020-06-16 DOI: 10.2174/1874070702014010078
Miluska Cisneros‐Yupanqui, A. Lante
{"title":"Tea from the Food Science Perspective: An Overview","authors":"Miluska Cisneros‐Yupanqui, A. Lante","doi":"10.2174/1874070702014010078","DOIUrl":"https://doi.org/10.2174/1874070702014010078","url":null,"abstract":"Tea (Camelia sinensis L.) is one of the main beverages known and consumed all around the world. Quality of tea is not only linked to the raw material but also to the processing steps that influence on the biochemical and sensory characteristics of each type of tea. This overview is focused on the differences in the production and composition of the main types of teas present in the market, highlighting not only their chemical and sensory characteristics, but also the importance of this plant from the food science viewpoint related to its several applications.","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"112 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114831480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Agricultural Wastes For Electricity Generation Using Microbial Fuel Cells 利用微生物燃料电池发电的农业废弃物
The Open Biotechnology Journal Pub Date : 2020-06-01 DOI: 10.2174/1874070702014010052
Segundo Jonathan Rojas Flores, Renny Nazario Naveda, Evelyn Paredes, Jessica Alza Orbegoso, Tiffany Cruz Céspedes, Angie Rodríguez Salvatierra, Milagros Sánchez Rodríguez
{"title":"Agricultural Wastes For Electricity Generation Using Microbial Fuel Cells","authors":"Segundo Jonathan Rojas Flores, Renny Nazario Naveda, Evelyn Paredes, Jessica Alza Orbegoso, Tiffany Cruz Céspedes, Angie Rodríguez Salvatierra, Milagros Sánchez Rodríguez","doi":"10.2174/1874070702014010052","DOIUrl":"https://doi.org/10.2174/1874070702014010052","url":null,"abstract":"The voltage measurements for the onion-based cell showed an upward trend that reaches a peak of 1.01 volts on the last day. Moreover, the greatest current generation was observed in onion cells, in which the current gradually increases from 10.2 to 24.7 mA on the last day. On the other hand, in all substrates, pH ranged from 7.5 and 10, which indicates the slightly alkaline behavior of the solutions.","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125300923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
A Comparative Proteomic Study of Thermobifida Cellulosilytica TB100T Secretome Grown on Carboxymethylcellulose and Rice Straw 羧甲基纤维素和水稻秸秆培养热裂菌TB100T分泌组的比较蛋白质组学研究
The Open Biotechnology Journal Pub Date : 2020-06-01 DOI: 10.2174/1874070702014010042
Mai H. Elmahdy, A. Azmy, E. El-Gebaly, Amal E. Saafan, Yasser Gaber
{"title":"A Comparative Proteomic Study of Thermobifida Cellulosilytica TB100T Secretome Grown on Carboxymethylcellulose and Rice Straw","authors":"Mai H. Elmahdy, A. Azmy, E. El-Gebaly, Amal E. Saafan, Yasser Gaber","doi":"10.2174/1874070702014010042","DOIUrl":"https://doi.org/10.2174/1874070702014010042","url":null,"abstract":"\u0000 \u0000 Cellulose, the major component of the plant cell wall, is the most abundant and cheap polymer on earth. It can be used by varieties of cellulolytic enzymes. Cellulases can hydrolyze cellulose to its glucose monomers, which can be fermented to many biotechnological products, such as biochemicals, bioplastics, and biofuels. Actinomycetes are potential sources of cellulases.\u0000 \u0000 \u0000 \u0000 The current study sheds light on the cellulolytic activity of Thermobifida cellulosilytica, a previously isolated thermophilic actinomycete, and the analysis of the lignocellulases produced in the secretome as a result of induction by different carbon sources.\u0000 \u0000 \u0000 \u0000 The cellulolytic activity was qualitatively confirmed by Congo red method showing a large halo zone around the colonies. The activity was also assayed using the 3,5-dinitrosalicylic acid (DNS) method. The secretome analysis was conducted by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) based proteomic approach.\u0000 \u0000 \u0000 \u0000 The cellulolytic activity increased by two folds upon the growth of T. cellulosilytica on rice straw (RS) as a complex substrate comparatively to Carboxymethylcellulose (CMC) as a simple one. These results were highly assured by LC-MS/MS. Where more proteins (n=31) were produced in the RS secretome, CMC produced only six proteins, including only one cellulase. Different classes of proteins produced in the RS secretome were cellulases (26%), hemicellulases (16%), proteases (10%), and others (48%).\u0000 \u0000 \u0000 \u0000 Lignocellulases are inducible enzymes. RS as a complex substrate induced T. cellulosilytica for the expression of more lignocellulolytic enzymes than CMC.\u0000","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115219711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Citric Acid Production by the Solid-State Cultivation Consortium of Aspergillus Niger and Trichoderma Reesei from Sugarcane Bagasse 甘蔗渣中黑曲霉和里氏木霉固态培养联合体生产柠檬酸的研究
The Open Biotechnology Journal Pub Date : 2020-03-29 DOI: 10.2174/1874070702014010032
R. G. Bastos, H. Ribeiro
{"title":"Citric Acid Production by the Solid-State Cultivation Consortium of Aspergillus Niger and Trichoderma Reesei from Sugarcane Bagasse","authors":"R. G. Bastos, H. Ribeiro","doi":"10.2174/1874070702014010032","DOIUrl":"https://doi.org/10.2174/1874070702014010032","url":null,"abstract":"Solid-State Cultivation (SSC) may be defined as the microbial growth on solid supports in conditions of the absence of free water, i.e., similar to the occurrence of fungi found in nature [1]. This process has been highlighted in chemical, food, pharmaceutical and agricultural industries as it requires little power, produces low-waste and reuses industrial residues as substrates having advantages when compared to submerged fermentation as it produces more concentrated product and higher yield in some metabolites [2]. Among the many microorganisms that can be grown from solid supports, those distinguished by filamentous fungi have the best ability to grow in these conditions due to their physiological and biochemical characteristics [3]. After phycomycetes (Mucor","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125199843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Upregulation of Antioxidant Gene Expressions and Enzyme Activity Against Acrylamide-Induced Neurotoxicity in Mice after Grape Seed Extract Treatment 葡萄籽提取物对丙烯酰胺诱导的小鼠神经毒性抗氧化基因表达和酶活性的上调
The Open Biotechnology Journal Pub Date : 2020-02-13 DOI: 10.2174/1874070702014010023
Sarah M. Albogami
{"title":"Upregulation of Antioxidant Gene Expressions and Enzyme Activity Against Acrylamide-Induced Neurotoxicity in Mice after Grape Seed Extract Treatment","authors":"Sarah M. Albogami","doi":"10.2174/1874070702014010023","DOIUrl":"https://doi.org/10.2174/1874070702014010023","url":null,"abstract":"Gpx1 (P < 0.05), Prdx3 (P < 0.01), SOD1 (P < 0.05), and CAT (P < 0.05) significantly upregulated in GSE-treated mice, compared to those in untreated controls. In contrast, Gpx1 (P < 0.05), Prdx3 (P < 0.05), SOD1 (P < 0.05), and CAT (P < 0.05) significantly downregulated in acrylamide-treated mice compared to those in untreated controls. Results of the treatment with GSE before exposure to acrylamide or simultaneously with acrylamide indicated that GSE restored Gpx1, Prdx3, SOD1, and CAT expression to similar levels as those in the control group. GSE treatment after exposure to acrylamide did not exert any neuroprotective effects against acrylamide, as revealed by significant downregulation of Gpx1 (P < 0.05), Prdx3 (P < 0.01), SOD1 (P < 0.05), and CAT (P < 0.05) compared to that in untreated controls. Animals treated with grape seed before acrylamide treatment showed no significant change in LPO activities and a significant increase in GSH levels, compared to those in untreated controls.","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115988214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effect of Plant Spacing and Np Fertilizer Levels on Growth, Seed Yield and Quality of Onion (Allium cepa L.) at Shewa Robit, Northern Ethiopia 衣索比亚北部Shewa Robit种植间距和氮肥水平对洋葱生长、产量和品质的影响
The Open Biotechnology Journal Pub Date : 2020-02-13 DOI: 10.2174/1874070702014010012
Getachew Amare, W. Mohammed, T. Tana
{"title":"Effect of Plant Spacing and Np Fertilizer Levels on Growth, Seed Yield and Quality of Onion (Allium cepa L.) at Shewa Robit, Northern Ethiopia","authors":"Getachew Amare, W. Mohammed, T. Tana","doi":"10.2174/1874070702014010012","DOIUrl":"https://doi.org/10.2174/1874070702014010012","url":null,"abstract":"The highest seed yield per hectare (879.4 kg) and per plot (663.6 g) was obtained from 115 P2O5 & 114 N kg ha -1 fertilizers and plant spacing 10×30cm gives the highest seed yield per plot (561.7g) and per hectare (748.9 kg). The highest germination percentage was obtained by the interaction effect of 10×30 cm and 143.6 P2O5 & 142.5 N kg ha . Whereas, highest seed vigor index I and II were obtained from the interaction of 115 P2O5 &114 N kg ha -1 and 20×30×50 cm spacing.","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114264731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Functional and Structural Characterization of a Novel Isoamylase from Ostreococcus tauri and Role of the N-Terminal Domain 一种新型牛链球菌异淀粉酶的功能和结构特征及n端结构域的作用
The Open Biotechnology Journal Pub Date : 2020-02-12 DOI: 10.2174/1874070702014010001
Nicolás Hedin, Julieta Barchiesi, D. Gomez-Casati, M. V. Busi
{"title":"Functional and Structural Characterization of a Novel Isoamylase from Ostreococcus tauri and Role of the N-Terminal Domain","authors":"Nicolás Hedin, Julieta Barchiesi, D. Gomez-Casati, M. V. Busi","doi":"10.2174/1874070702014010001","DOIUrl":"https://doi.org/10.2174/1874070702014010001","url":null,"abstract":"Carbohydrates are the most abundant molecules on earth; each year photosynthesis fixes approximately 100 billion tons of CO2 and H2O into cellulose, starch, sucrose and other sugars. Starch is one of the most important sources of energy in the human diet. It is composed of two polymers, amylose (essentially an α-1,4-glucan chain) and amylopectin (similar to amylose but branched with α-1,6 glycosidic bonds) whose combination gives rise to a highly insoluble starch granule [1, 2]. Amylo-","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129276642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Growth and Yield Response of Garlic (Allium Sativum L.) to Intra-row Spacing and Variety at Selekeleka, Northern Ethiopia 埃塞俄比亚北部Selekeleka地区大蒜生长和产量对行距和品种的响应
The Open Biotechnology Journal Pub Date : 2020-02-02 DOI: 10.2174/1874070702115010001
Mulu Teshale, Negasi Tekeste
{"title":"Growth and Yield Response of Garlic (Allium Sativum L.) to Intra-row Spacing and Variety at Selekeleka, Northern Ethiopia","authors":"Mulu Teshale, Negasi Tekeste","doi":"10.2174/1874070702115010001","DOIUrl":"https://doi.org/10.2174/1874070702115010001","url":null,"abstract":"Background: Garlic is an important condiment and cash crop in Ethiopia. Low yield and productivity of the crop are the major characteristics of the crop in the country. Many factors affect the productivity of the crop in the country, however poor agronomic practices, especially the use of low-yielding varieties and inappropriate spacing, are the major ones.","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131863928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Application of CRISPR/Cas9 Genome Editing System in Cereal Crops CRISPR/Cas9基因组编辑系统在谷类作物中的应用
The Open Biotechnology Journal Pub Date : 2019-12-11 DOI: 10.2174/1874070701913010173
V. E. Hillary, S. Ceasar
{"title":"Application of CRISPR/Cas9 Genome Editing System in Cereal Crops","authors":"V. E. Hillary, S. Ceasar","doi":"10.2174/1874070701913010173","DOIUrl":"https://doi.org/10.2174/1874070701913010173","url":null,"abstract":"Recent developments in targeted genome editing accelerated genetic research and opened new potentials to improve the crops for better yields and quality. Genome editing techniques like Zinc Finger Nucleases (ZFN) and Transcription Activator-Like Effector Nucleases (TALENs) have been accustomed to target any gene of interest. However, these systems have some drawbacks as they are very expensive and time consuming with labor-intensive protein construction protocol. A new era of genome editing technology has a user-friendly tool which is termed as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated protein9 (Cas9), is an RNA based genome editing system involving a simple and cost-effective design of constructs. CRISPR/Cas9 system has been successfully applied in diverse crops for various genome editing approaches. In this review, we highlight the application of the CRISPR/Cas9 system in cereal crops including rice, wheat, maize, and sorghum to improve these crops for better yield and quality. Since cereal crops supply a major source of food to world populations, their improvement using recent genome editing tools like CRISPR/Cas9 is timely and crucial. The genome editing of cereal crops using the CRISPR/Cas9 system would help to overcome the adverse effects of agriculture and may aid in conserving food security in developing countries.","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"188 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132824684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
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