Supachai Hirunsupachote, W. Deelaman, Ronnapop Putrakulpattana, Krittikan Sarapirom, Palchat Wongkaew
{"title":"Efficiency of wastewater treatment from environmental laboratory of science and technology, RMUTP using ozonation","authors":"Supachai Hirunsupachote, W. Deelaman, Ronnapop Putrakulpattana, Krittikan Sarapirom, Palchat Wongkaew","doi":"10.60101/jarst.2023.254126","DOIUrl":"https://doi.org/10.60101/jarst.2023.254126","url":null,"abstract":"Wastewater from the laboratory in the Division of Environmental Science and Technology, Faculty of Science and Technology, Rajamangala University of Technology Phra Nakhon is a source of pollution without any treatment. Researchers realized that it would become an environmental problem in the future. For this reason, wastewater treatment from water laboratory would be studied using ozonation process because the researchers want to use the existing equipment in the laboratory and a simple technique that the students can do. This aims to treat the wastewater until it passes the water standard. The laboratory's wastewater characteristics were unclear based on the study. The pH values measured were 8.15, 3.35, and 2.49, respectively. COD levels were monitored of 10,480, 2,400, and 3,040 mg/L, while the measured BOD values were 4,649, 1,683, and 2,200 mg/L, respectively. Additionally, the SS concentrations were 682, 788, and 548 mg/L, respectively. Because of these characteristics using only the biodegradation process didn’t work. The ozonation techniques were a good option following the research objective. The results showed that the suitable condition for the ozonation process was pH6 and the optimal time was 40 minutes. Because after 40 minutes the COD removal increase only 5% but after 60 minutes the ozonation had no effect on COD removal. This condition was applied before using biological treatment via a sequence batch reactor (SBR). Using microorganisms and 8 hours of aeration via SBR, the efficiency of COD BOD and SS removal was only 41.66, 0 and 71.82 percent, respectively. Filling ozone in rate 0.25 gram/hour/liter wastewater with SBR reactor, the wastewater treatment efficiency increased. It can remove COD, BOD and SS of 63.15, 50.00 and 72.63 percent, respectively. The advantage of this research was that the ozone generator can be applied for wastewater treatment without additional costs.","PeriodicalId":290694,"journal":{"name":"Journal of Applied Research on Science and Technology (JARST)","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Phongsupha Chanthachaiyaphum, Somporn Moonmangmee, D. Moonmangmee
{"title":"2-Keto-gluconate production and purification by thermotolerant acetic acid bacterium Nguyenibacter vanlangensis KKS-R1","authors":"Phongsupha Chanthachaiyaphum, Somporn Moonmangmee, D. Moonmangmee","doi":"10.60101/jarst.2023.254506","DOIUrl":"https://doi.org/10.60101/jarst.2023.254506","url":null,"abstract":"2-Keto-gluconic acid (2-KGA) is a pivotal intermediate in the production process of ascorbic acid, commonly known as vitamin C. The bacterium Nguyenibacter vanlangensis KKS-R1 is an acetic acid bacterium (AAB) that has been selectively screened for its ability to produce 2-KGA among twenty-five bacterial isolates at high temperatures up to 40°C. This thermotolerant property makes it advantageous for various industries. Therefore, it has garnered interest for its potential application in the production of 2-KGA and purification of the 2-KGA product. This study evaluated the production of 2-KGA by N. vanlangensis KKS-R1 using 1% gluconate as the substrate. The 2-KGA was determined by thin-layer chromatography, Lanning and Cohen’s method, and high-performance liquid chromatography. After fermentation in a minimal liquid medium for 24 hours, the bacterium showcased its prowess by producing 2-KGA concentrations of 7.5 g/L. However, the purity of the 2-KGA production is paramount for industrial applications. In addressing this, a rigorous chromatographic purification regimen was employed. This involved two sequential stages of ion exchange chromatography, DEAE-Sephacel and DOWEX 1X4, followed by SuperdexTM S-200 column chromatography. The collective efficacy of these methodologies yielded a product with an impressive purity index of 71.02%. The 2-KGA product was then subjected to a decolorization process using activated carbon and freeze-dried. This results in the compound being a pure white powder. This investigation indicates that N. vanlangensis KKS-R1 shows good potential as a 2-KGA producer at high temperatures. Under proper purification techniques. These findings contribute to developing purified processes and producing 2-KGA for industrial efficiency.","PeriodicalId":290694,"journal":{"name":"Journal of Applied Research on Science and Technology (JARST)","volume":" 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138963012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Watcharin Yuttavanichakul, N. Kanthong, Nuntaporn Pungsungvorn
{"title":"Boosting cordycepin production through plant-based oils for vegetarian consumption","authors":"Watcharin Yuttavanichakul, N. Kanthong, Nuntaporn Pungsungvorn","doi":"10.60101/jarst.2023.254104","DOIUrl":"https://doi.org/10.60101/jarst.2023.254104","url":null,"abstract":"Cordyceps fungi, including species such as Cordyceps sinensis and Cordyceps militaris, are known for producing bioactive chemicals, notably cordycepin. Traditional cordyceps cultivation in Thailand relies on silkworm pupae as a substrate in solid-state fermentation, posing challenges in catering to vegetarian consumers. This study aimed to develop a solid-state fermentation process for cordyceps cultivation using vegetable oils, thus modifying the cereals medium and eliminating the need for silkworm pupae while enhancing bioactive chemical production and promoting cordyceps growth. The findings demonstrate that C. militaris can successfully grow and produce adenosine and cordycepin when the culture medium is modified with plant oils. Plant oils, including olive, soybean, peanut, palm, sesame, coconut, and sunflower oil, proved effective for cultivating C. militaris on PDA. Notably, adding a 3% mixture of palm oil in PDA resulted in the most significant promotion of C. militaris mycelium, with a diameter of 5.93 cm. Consequently, the modified cereals medium, incorporating palm oil, was adopted for solid-state fermentation of C. militaris. The results demonstrate that C. militaris can be successfully cultured to produce fruiting bodies comparable and total yields to those obtained using traditional cereals and silkworm pupa medium. Furthermore, there is a notable increase in adenosine and cordycepin production, indicating the potential of this method to enhance C. militaris yield and bioactive chemical output. This research highlights the feasibility of incorporating plant oils as substitutes or additives to silkworm pupae, improving productivity and enabling the production of C. militaris suitable for vegetarian consumption.","PeriodicalId":290694,"journal":{"name":"Journal of Applied Research on Science and Technology (JARST)","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139243675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cellulose rubber foam composite use as oil absorbent","authors":"Poptorn Klaykhem, Pruttipong Pantamanatsopaopa, Warunee Ariyawiriyanan","doi":"10.60101/jarst.2023.254006","DOIUrl":"https://doi.org/10.60101/jarst.2023.254006","url":null,"abstract":"The focus of this study was to explore the fabrication of cellulose rubber foam (CRF) using kapok fibers (KF) as an oil absorbent material. Chemical methods such as sodium hydroxide surface treatment, hydrogen peroxide treatment, and acid hydrolysis were employed to prepare cellulose nanocrystals (CNC). The results of the nuclear magnetic resonance (NMR) spectroscopy test indicated that the chemical modification of kapok fiber resulted in the removal of lignin and hemicellulose by a disappearing peak at 17, 52, and 148 ppm, respectively. Hydrolysis process of the kapok fiber resulted in nanometer-sized cellulose, with a yield of 72% as revealed by transmission electron microscopy (TEM). The amount of cellulose nanocrystals from kapok fiber (KF-CNC) used in the study varied from 0 to 5 phr during the formation of the cellulose rubber foam, and it was found that the foam density increased as the number of cellulose nanocrystals from kapok fiber increased. Additionally, the percentage of collapse from the compressive strength of cellulose rubber foam decreased as the amount of cellulose nanocrystals from kapok fiber increased. Fourier transform infrared spectroscopy (FTIR) confirmed the incorporation of cellulose nanocrystals from kapok fiber into the rubber foam (RF) as the amount of cellulose nanocrystals from kapok fiber increased. The oil absorbent of cellulose rubber foam composite with 1 phr cellulose nanocrystals from kapok fiber show highest absorption capacity was 17.8 g/g. The cellulose rubber foam composite absorbs oil before absorbing water when water and oil are combined. Moreover, the cellulose rubber foam could be reused more than 50 times.","PeriodicalId":290694,"journal":{"name":"Journal of Applied Research on Science and Technology (JARST)","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139265254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Further investigation on fixed point theorems via C-class functions in extended b-metric spaces","authors":"T. Khanpanuk, Chainarong Khunpanuk","doi":"10.60101/jarst.2023.251308","DOIUrl":"https://doi.org/10.60101/jarst.2023.251308","url":null,"abstract":"The purpose of this research project is to develop new theories, discuss, and extend some recent common fixed point results established when the underlying ambient space is an extended b-metric space and the contraction condition involves a new class of ψ-φ-C-contraction type mappings where ψ is the altering distance function and φ is the ultra-altering distance function. The unique fixed point theorems for such mappings in the setting of ψ-φ-complete metric spaces are proven. We also prove the fixed point theorem in partially ordered metric spaces. Moreover, some examples supporting the main results are given. Our results extend and generalize corresponding results in the literature. The start of the development of the theory of fixed points is tied to the end of the 19th century. The method of successive approximations is used in order to prove the solution's existence and uniqueness at the beginning of differential and integral equations. This branch of nonlinear analysis has been developed through various classes of spaces, such as metric spaces, topological spaces, probabilistic metric spaces, fuzzy metric spaces, and others. In developing the theory of fixed points, achievements are applied in various sciences, such as optimization, economics, and approximation theory. A very important step in the development of fixed point theory was taken by A.H. Ansari through the introduction of a C-class function. Using C-class functions, we generalize some known fixed point results, and Kamran et al. introduced a new intuitive concept of distance measure to extend the notion of b-metric space by further weakening the triangle inequality.","PeriodicalId":290694,"journal":{"name":"Journal of Applied Research on Science and Technology (JARST)","volume":"24 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132605172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mixed Bootstrap-Marascuilo test for testing equality of means under unequal variances in a completely randomized design","authors":"พรรณิภา วรพันธ์, Tammarat Kleebmek","doi":"10.60101/jarst.2023.251663","DOIUrl":"https://doi.org/10.60101/jarst.2023.251663","url":null,"abstract":"This study introduces a pioneering statistical methodology, the Mixed Bootstrap-Marascuilo test, which emerges from the fusion of the Bootstrap method and the Marascuilo test. The test examines the means of three or more populations with unequal variances in a completely randomized design. The research compares the Mixed Bootstrap-Marascuilo test's ability to control the probability of type I error using Cochran's criteria and its test power using penalized power with those of One-Way ANOVA and the Marascuilo test. The research design thoughtfully encompasses three populations: small, medium, and large, each exhibiting variations in size and equality. It systematically manipulates variances, encompassing a range from equal to slightly different, moderately different, and significantly different. Furthermore, the error distribution is specified to be normal with a designated mean. The research methodology embraces the utilization of R ver.4.2.2 and the Monte Carlo technique, leveraging 5,000 simulations per case to ensure the robustness and reliability of the study's findings. The outcomes of the comprehensive analysis yield intriguing insights. The results indicate that the Mixed Bootstrap-Marascuilo test exhibits superior testing ability in scenarios involving equal small sample sizes with equal and moderate variances, as well as medium sample sizes with equal and small variances. Furthermore, it demonstrates effectiveness even when dealing with large, unequal sample sizes with equal and large variances. In essence, this research advances the realm of statistical hypothesis testing through the introduction and meticulous evaluation of the Mixed Bootstrap-Marascuilo test. Its demonstrated ability to navigate complexities in mean examination across diverse populations, coupled with its versatile applicability to scenarios of unequal variances and varying sample sizes, underscores its potential as a valuable tool for researchers across disciplines.","PeriodicalId":290694,"journal":{"name":"Journal of Applied Research on Science and Technology (JARST)","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124822638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Smart meter design for energy consumption monitoring of residential premises","authors":"Muhammad Mansattha, H. Dao, Arfip Jikaraji","doi":"10.60101/jarst.2023.250745","DOIUrl":"https://doi.org/10.60101/jarst.2023.250745","url":null,"abstract":"Electricity consumption is a crucial issue for economic development, especially in the residential sector. The number of households has increased significantly despite the declining availability of energy resources. There is a clear need for an efficient electricity consumption monitoring system that can provide accurate data on residential energy consumption. This paper proposes an Internet of Things (IoT)-based smart meter system design for monitoring household energy consumption. The system employs hardware and IoT technology, specifically the Node-MCU of ESP-32 and an ADE7757 power sensor module which runs on a 5(15) Amp of a single-phase meter. The energy consumption data is logged in cloud storage using the AppSheet platform hosted on Google Cloud. The system features digital displays and consumption analytics, allowing consumers to collect and transmit data about their energy usage. Energy usage data provides consumers accurate and timely information about their energy consumption. This information can help them better manage and reduce their energy usage. The system also offers the estimation of the energy consumption for individual appliances with a user-friendly monitoring experience for the energy sector. The proposed smart meter system has been evaluated using 1,080 data sets, with an average accuracy rate of 1.48% compared to a 5(15) Amp, single-phase meter. Additionally, the system can predict energy charges with an accuracy of 0.02% based on the schedule of residential electricity tariff regulated by the Provincial Electricity Agency (PEA), Thailand. These results show that the system is highly accurate and can promote positive user behavior towards better energy supply and demand management, reduced energy waste, and improved system reliability. The features of smart meter systems enable consumers and utilities to make more informed decisions about energy usage, promoting more efficient and sustainable energy practices.","PeriodicalId":290694,"journal":{"name":"Journal of Applied Research on Science and Technology (JARST)","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131325519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Extraction of natural dye from Oroxylumb indicum (L.) Kurz by using different solvents for Thai eri silk dyeing","authors":"Aritart Sanai, Thippawan Nachai, Kesorn Merat","doi":"10.60101/jarst.2023.251099","DOIUrl":"https://doi.org/10.60101/jarst.2023.251099","url":null,"abstract":"Among various textile types in Thailand, silk is the largest market share. Thus, upgrading or developing either the dying process or the quality of fabric dried for Thai silk is still necessary. Natural dyes have become an interesting resource because they are more environmentally friendly than synthetic dyes. Thus, this work aims to study the effect of different solvents for dye extraction from Oroxylumb Indicum (L.) Kurz on Eri Thai silk dyeing. Each solvent used for extraction gave a different relative tannic acid and quercetin content. Therefore, this further leads to different properties of dyed fabrics, such as color properties and washing fastness index. From the different solvents studied, the yield of the extracted dyes was on the order of EtOH/water (50: 50) > MeOH/water (50:50) > MeOH ~ EtOH > water. Then, the optimal ratios between EtOH and water in the efficient extraction were varied as follows water, EtOH/water (25: 75), EtOH/water (50: 50), EtOH/water (75:25), and EtOH. The result showed that EtOH/water (50: 50) was still the most effective for dye extraction. In addition, the effect of dose, temperature, and time of Oroxylumb Indicum (L.) Kurz was 8.0 g/L, 60 °C, and 60 min, which were considered optimal extraction conditions due to the high relative contents of tannic acid and quercetin. As a result, the fabric of Eri silk dyed has a high quality of fastness rating. Overall, these results could point out the use of extracted dye from Oroxylumb Indicum (L.) Kurz has the potential as an alternative dye for Eri Thai silk dyeing.","PeriodicalId":290694,"journal":{"name":"Journal of Applied Research on Science and Technology (JARST)","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132843576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}