Extremophiles : life under extreme conditions最新文献_第4页

Cell surface display of cold-active esterase EstPc with the use of a new autotransporter from Psychrobacter cryohalolentis K5(T). 利用低温嗜盐杆菌K5(T)的一种新的自转运体，展示冷活性酯酶EstPc的细胞表面。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2015-01-01 Epub Date: 2014-09-25 DOI: 10.1007/s00792-014-0695-0

L E Petrovskaya, K A Novototskaya-Vlasova, E A Kryukova, E M Rivkina, D A Dolgikh, M P Kirpichnikov

{"title":"Cell surface display of cold-active esterase EstPc with the use of a new autotransporter from Psychrobacter cryohalolentis K5(T).","authors":"L E Petrovskaya, K A Novototskaya-Vlasova, E A Kryukova, E M Rivkina, D A Dolgikh, M P Kirpichnikov","doi":"10.1007/s00792-014-0695-0","DOIUrl":"https://doi.org/10.1007/s00792-014-0695-0","url":null,"abstract":"We have cloned the gene coding for AT877-a new predicted member of the autotransporter protein family with an esterase passenger domain from permafrost bacterium Psychrobacter cryohalolentis K5(T). Expression of AT877 gene in Escherichia coli resulted in accumulation of the recombinant autotransporter in the outer membrane fraction and at the surface of the induced cells. AT877 displayed maximum hydrolytic activity toward medium-chain p-nitrophenyl esters (C8-C10) at 50 °C and was resistant to the presence of several metal ions, organic solvents and detergents. Previously, we have described a cold-active esterase EstPc from the same bacterium which possesses high activity at low temperatures and relatively high thermal stability. To construct a cell surface display system for EstPc, the hybrid autotransporter gene coding for EstPc with the α-helical linker and the translocator domain from AT877 was constructed and expressed in E. coli. According to the results of the cell fractionation studies and esterase activity measurements, the EstPc passenger was successfully displayed at the surface of the induced cells. It demonstrated a temperature optimum at 15-25 °C and a substrate preference toward p-nitrophenyl butyrate (C4). Obtained results provide a new example of the biotechnologically relevant enzyme from the permafrost microbial community with potential applications for the conversion of short- and medium-chain ester substrates and a basis for the construction of a new cell surface display platform. ","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"161-70"},"PeriodicalIF":2.9,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00792-014-0695-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32694442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Community succession of bacteria and eukaryotes in dune ecosystems of Gurbantünggüt Desert, Northwest China. 古尔班塔<s:1> ngg<e:1>沙漠沙丘生态系统细菌和真核生物群落演替

Extremophiles : life under extreme conditions Pub Date : 2015-01-01 Epub Date: 2014-09-25 DOI: 10.1007/s00792-014-0696-z

Ke Li, Zhihui Bai, Hongxun Zhang

{"title":"Community succession of bacteria and eukaryotes in dune ecosystems of Gurbantünggüt Desert, Northwest China.","authors":"Ke Li, Zhihui Bai, Hongxun Zhang","doi":"10.1007/s00792-014-0696-z","DOIUrl":"10.1007/s00792-014-0696-z","url":null,"abstract":"Pyrosequencing and quantitative polymerase chain reaction of small subunit rRNA genes were used to provide a comprehensive examination of bacterial, cyanobacterial, and eukaryotic communities in the biological soil crusts (BSCs) of Gurbantünggüt Desert sand dunes (China). Three succession stages were recognized based on the analyses of eukaryotic communities: a late succession stage of BSCs in a swale with eukaryotes mainly related to the Bryophyta clade, an initial succession stage in a slope with barely any eukaryotic phototrophic microorganisms detected, and an intermediate succession type detected from both the swale and slope BSCs dominated by the phylum Chlorophyta. Moreover, the cyanobacterial community dominated all of the BSCs (48.2-69.5% of the total bacteria) and differed among the three succession stages: sequences related to Microcoleus steenstrupii and the genus Scytonema were abundant in the later succession stage, whereas both the initial and intermediate stages were dominated by Microcoleus vaginatus. Compared with swales, BSCs from slopes are exposed to a harsher environment, e.g., higher irradiance and lower water availability, and thus may be restricted from developing to a higher succession stage. Other disturbances such as wind and grazing may explain the different succession stages observed in swales or slopes. However, no clear differences were detected from non-phototrophic bacterial communities of the three succession stages, and sequences related to Alphaproteobacteria and Actinobacteria were most abundant in all the BSCs. The closest matches for the most frequent non-phototrophic bacterial genera were mainly derived from harsh environments, indicating the robustness of these genera.","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"171-81"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32694443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum. 非嗜盐嗜酸菌隐酸菌的羟基外托因基因簇。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2015-01-01 Epub Date: 2014-08-21 DOI: 10.1007/s00792-014-0687-0

Katharina D Moritz, Birgit Amendt, Elisabeth M H J Witt, Erwin A Galinski

{"title":"The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum.","authors":"Katharina D Moritz, Birgit Amendt, Elisabeth M H J Witt, Erwin A Galinski","doi":"10.1007/s00792-014-0687-0","DOIUrl":"https://doi.org/10.1007/s00792-014-0687-0","url":null,"abstract":"Acidiphilium cryptum is an acidophilic, heterotrophic α-Proteobacterium which thrives in acidic, metal-rich environments (e.g. acid mine drainage). Recently, an ectABCDask gene cluster for biosynthesis of the compatible solutes ectoine and hydroxyectoine was detected in the genome sequence of A. cryptum JF-5. We were able to demonstrate that the type strain A. cryptum DSM 2389(T) is capable of synthesizing the compatible solute hydroxyectoine in response to moderate osmotic stress caused by sodium chloride and aluminium sulphate, respectively. Furthermore, we used the A. cryptum JF-5 sequence to amplify the ectABCDask gene cluster from strain DSM 2389(T) and achieved heterologous expression of the gene cluster in Escherichia coli. Hence, we could for the first time prove metabolic functionality of the genes responsible for hydroxyectoine biosynthesis in the acidophile A. cryptum. In addition, we present information on specific enzyme activity of A. cryptum DSM 2389(T) ectoine synthase (EctC) in vitro. In contrast to EctCs from halophilic microorganisms, the A. cryptum enzyme exhibits a higher isoelectric point, thus a lower acidity, and has maximum specific activity in the absence of sodium chloride. ","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"87-99"},"PeriodicalIF":2.9,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00792-014-0687-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32601353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Desulfosporosinus acididurans sp. nov.: an acidophilic sulfate-reducing bacterium isolated from acidic sediments. 酸性desulfosporosinusacididurans sp. nov.:一种从酸性沉积物中分离出来的嗜酸硫酸盐还原细菌。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2015-01-01 Epub Date: 2014-11-05 DOI: 10.1007/s00792-014-0701-6

Irene Sánchez-Andrea, Alfons J M Stams, Sabrina Hedrich, Ivan Ňancucheo, D Barrie Johnson

{"title":"Desulfosporosinus acididurans sp. nov.: an acidophilic sulfate-reducing bacterium isolated from acidic sediments.","authors":"Irene Sánchez-Andrea, Alfons J M Stams, Sabrina Hedrich, Ivan Ňancucheo, D Barrie Johnson","doi":"10.1007/s00792-014-0701-6","DOIUrl":"https://doi.org/10.1007/s00792-014-0701-6","url":null,"abstract":"Three strains of sulfate-reducing bacteria (M1(T), D, and E) were isolated from acidic sediments (White river and Tinto river) and characterized phylogenetically and physiologically. All three strains were obligately anaerobic, mesophilic, spore-forming straight rods, stained Gram-negative and displayed variable motility during active growth. The pH range for growth was 3.8-7.0, with an optimum at pH 5.5. The temperature range for growth was 15-40 °C, with an optimum at 30 °C. Strains M1(T), D, and E used a wide range of electron donors and acceptors, with certain variability within the different strains. The nominated type strain (M1(T)) used ferric iron, nitrate, sulfate, elemental sulfur, and thiosulfate (but not arsenate, sulfite, or fumarate) as electron acceptors, and organic acids (formate, lactate, butyrate, fumarate, malate, and pyruvate), alcohols (glycerol, methanol, and ethanol), yeast extract, and sugars (xylose, glucose, and fructose) as electron donors. It also fermented some substrates such as pyruvate and formate. Strain M1(T) tolerated up to 50 mM ferrous iron and 10 mM aluminum, but was inhibited by 1 mM copper. On the basis of phenotypic, phylogenetic, and genetic characteristics, strains M1(T), D, and E represent a novel species within the genus Desulfosporosinus, for which the name Desulfosporosinus acididurans sp. nov. is proposed. The type strain is M1(T) (=DSM 27692(T) = JCM 19471(T)). Strain M1(T) was the first acidophilic SRB isolated, and it is the third described species of acidophilic SRB besides Desulfosporosinus acidiphilus and Thermodesulfobium narugense. ","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"39-47"},"PeriodicalIF":2.9,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00792-014-0701-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32792772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 108

A pectate lyase from a deep subseafloor Georgenia muralis with unusual molecular characteristics. 一种具有不同寻常分子特征的深海底海棠果胶裂解酶。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2015-01-01 Epub Date: 2014-09-11 DOI: 10.1007/s00792-014-0691-4

Masato Sasaki, Osamu Koide, Tohru Kobayashi, Ron Usami, Koki Horikoshi

引用次数: 6

Characterization of the frhAGB-encoding hydrogenase from a non-methanogenic hyperthermophilic archaeon. 非产甲烷的嗜热古菌frhagb编码氢化酶的特性研究。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2015-01-01 Epub Date: 2014-08-21 DOI: 10.1007/s00792-014-0689-y

Jeong Ho Jeon, Jae Kyu Lim, Min-Sik Kim, Tae-Jun Yang, Seong-Hyuk Lee, Seung Seob Bae, Yun Jae Kim, Sang Hee Lee, Jung-Hyun Lee, Sung Gyun Kang, Hyun Sook Lee

{"title":"Characterization of the frhAGB-encoding hydrogenase from a non-methanogenic hyperthermophilic archaeon.","authors":"Jeong Ho Jeon, Jae Kyu Lim, Min-Sik Kim, Tae-Jun Yang, Seong-Hyuk Lee, Seung Seob Bae, Yun Jae Kim, Sang Hee Lee, Jung-Hyun Lee, Sung Gyun Kang, Hyun Sook Lee","doi":"10.1007/s00792-014-0689-y","DOIUrl":"https://doi.org/10.1007/s00792-014-0689-y","url":null,"abstract":"The F420-reducing hydrogenase has been known as a key enzyme in methanogenesis. Its homologs have been identified in non-methanogenic hyperthermophilic archaea, including Thermococcus onnurineus NA1, but neither physiological function nor biochemical properties have been reported to date. The enzyme of T. onnurineus NA1 was distinguished from those of other methanogens and the members of the family Desulfurobacteriaceae with respect to the phylogenetic distribution of the α and β subunits, organization of frhAGB genes and conservation of F420-coordinating residues. RT-qPCR and Western blot analyses revealed frhA gene is not silent but is expressed in T. onnurineus NA1 grown in the presence of sulfur, carbon monoxide, or formate. The trimeric enzyme complex was purified to homogeneity via affinity chromatography from T. onnurineus NA1 and exhibited catalytic activity toward the electron acceptors such as viologens and flavins but not the deazaflavin coenzyme F420. This is the first biochemical study on the function of the frhAGB-encoding enzyme from a non-methanogenic archaea. ","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"109-18"},"PeriodicalIF":2.9,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00792-014-0689-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32601354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Discovery of bacterial polyhydroxyalkanoate synthase (PhaC)-encoding genes from seasonal Baltic Sea ice and cold estuarine waters. 从波罗的海季节性冰和寒冷的河口水域发现细菌聚羟基烷酸合成酶(PhaC)编码基因。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2015-01-01 Epub Date: 2014-10-04 DOI: 10.1007/s00792-014-0699-9

Katariina Pärnänen, Antti Karkman, Marko Virta, Eeva Eronen-Rasimus, Hermanni Kaartokallio

{"title":"Discovery of bacterial polyhydroxyalkanoate synthase (PhaC)-encoding genes from seasonal Baltic Sea ice and cold estuarine waters.","authors":"Katariina Pärnänen, Antti Karkman, Marko Virta, Eeva Eronen-Rasimus, Hermanni Kaartokallio","doi":"10.1007/s00792-014-0699-9","DOIUrl":"https://doi.org/10.1007/s00792-014-0699-9","url":null,"abstract":"Polyhydroxyalkanoates (PHAs) are macromolecules produced by bacteria as means for storing carbon and energy in intracellular granules. PHAs have physical properties similar to those of plastics and have become of interest to industry as materials for environmentally friendly bioplastic production. There is an ongoing search for new PHA-producing bacterial strains and PHA-synthesizing enzymes tolerating extreme conditions to find ways of producing PHAs at cold temperatures and high solute concentrations. Moreover, the study of PHA producers in the sea-ice biome can aid in understanding the microbial ecology of carbon cycling in ice-associated ecosystems. In this study, PHA producers and PHA synthase genes were examined under the extreme environmental conditions of sea ice and cold seawater to find evidence of PHA production in an environment requiring adaptation to high salinity and cold temperatures. Sea ice and cold estuarine water samples were collected from the northern Baltic Sea and evidence of PHA production was gathered, using microscopy with Nile Blue A staining of PHA-granules and PCR assays detecting PHA-synthesis genes. The PHA granules and PHA synthases were found at all sampling locations, in both sea ice and water, and throughout the sampling period spanning over 10 years. Our study shows, for the first time, that PHA synthesis occurs in Baltic Sea cold-adapted bacteria in their natural environment, which makes the Baltic Sea and its cold environments an interesting choice in the quest for PHA-synthesizing bacteria and synthesis genes. ","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"197-206"},"PeriodicalIF":2.9,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00792-014-0699-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32718395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Description of gamma radiation-resistant Geodermatophilus dictyosporus sp. nov. to accommodate the not validly named Geodermatophilus obscurus subsp. dictyosporus (Luedemann, 1968). 描述抗伽玛辐射的双芽地皮ophilus dictyosporus sp. 11 .以适应未被有效命名的暗色地皮ophilus subsp。dictyosporus （Luedemann, 1968）。

Extremophiles : life under extreme conditions Pub Date : 2015-01-01 Epub Date: 2014-11-16 DOI: 10.1007/s00792-014-0708-z

Maria del Carmen Montero-Calasanz, Karima Hezbri, Markus Göker, Haïtham Sghaier, Manfred Rohde, Cathrin Spröer, Peter Schumann, Hans-Peter Klenk

{"title":"Description of gamma radiation-resistant Geodermatophilus dictyosporus sp. nov. to accommodate the not validly named Geodermatophilus obscurus subsp. dictyosporus (Luedemann, 1968).","authors":"Maria del Carmen Montero-Calasanz, Karima Hezbri, Markus Göker, Haïtham Sghaier, Manfred Rohde, Cathrin Spröer, Peter Schumann, Hans-Peter Klenk","doi":"10.1007/s00792-014-0708-z","DOIUrl":"10.1007/s00792-014-0708-z","url":null,"abstract":"A gamma radiation-resistant, Gram reaction-positive, aerobic and chemoorganotrophic actinobacterium, initially designated Geodermatophilus obscurus subsp. dictyosporus G-5(T), was not validly named at the time of initial publication (1968). G-5(T) formed black-colored colonies on GYM agar. The optimal growth range was 25-35 °C, at pH 6.5-9.5 and in the absence of NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G + C content of the strain was 75.3 mol%. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The main polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and one unspecified glycolipid; MK-9(H4) was the dominant menaquinone and galactose was detected as a diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids, iso-C16:0 and iso-C15:0. The 16S rRNA gene showed 94.8-98.4 % sequence identity with the members of the genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G-5(T) is proposed to represent a novel species, Geodermatophilus dictyosporus and the type strain is G-5(T) (=DSM 43161(T) = CCUG 62970(T) = MTCC 11558(T) = ATCC 25080(T) = CBS 234.69(T) = IFO 13317(T) = KCC A-0154(T) = NBRC 13317(T)). The INSDC accession number is HF970584.","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"77-85"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32815935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perchlorate and halophilic prokaryotes: implications for possible halophilic life on Mars. 高氯酸盐和嗜盐原核生物:对火星上可能的嗜盐生命的启示。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2014-01-01 Epub Date: 2013-10-23 DOI: 10.1007/s00792-013-0594-9

Aharon Oren, Rahel Elevi Bardavid, Lily Mana

{"title":"Perchlorate and halophilic prokaryotes: implications for possible halophilic life on Mars.","authors":"Aharon Oren, Rahel Elevi Bardavid, Lily Mana","doi":"10.1007/s00792-013-0594-9","DOIUrl":"https://doi.org/10.1007/s00792-013-0594-9","url":null,"abstract":"In view of the finding of perchlorate among the salts detected by the Phoenix Lander on Mars, we investigated the relationships of halophilic heterotrophic microorganisms (archaea of the family Halobacteriaceae and the bacterium Halomonas elongata) toward perchlorate. All strains tested grew well in NaCl-based media containing 0.4 M perchlorate, but at the highest perchlorate concentrations, tested cells were swollen or distorted. Some species (Haloferax mediterranei, Haloferax denitrificans, Haloferax gibbonsii, Haloarcula marismortui, Haloarcula vallismortis) could use perchlorate as an electron acceptor for anaerobic growth. Although perchlorate is highly oxidizing, its presence at a concentration of 0.2 M for up to 2 weeks did not negatively affect the ability of a yeast extract-based medium to support growth of the archaeon Halobacterium salinarum. These findings show that presence of perchlorate among the salts on Mars does not preclude the possibility of halophilic life. If indeed the liquid brines that may exist on Mars are inhabited by salt-requiring or salt-tolerant microorganisms similar to the halophiles on Earth, presence of perchlorate may even be stimulatory when it can serve as an electron acceptor for respiratory activity in the anaerobic Martian environment.","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"75-80"},"PeriodicalIF":2.9,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00792-013-0594-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40259601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 86

Isolation and characterization of phage-host systems from the Baltic Sea ice. 波罗的海海冰中噬菌体-宿主系统的分离和表征。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2014-01-01 Epub Date: 2013-12-03 DOI: 10.1007/s00792-013-0604-y

Anne-Mari Luhtanen, Eeva Eronen-Rasimus, Hermanni Kaartokallio, Janne-Markus Rintala, Riitta Autio, Elina Roine

{"title":"Isolation and characterization of phage-host systems from the Baltic Sea ice.","authors":"Anne-Mari Luhtanen, Eeva Eronen-Rasimus, Hermanni Kaartokallio, Janne-Markus Rintala, Riitta Autio, Elina Roine","doi":"10.1007/s00792-013-0604-y","DOIUrl":"https://doi.org/10.1007/s00792-013-0604-y","url":null,"abstract":"In search for sea ice bacteria and their phages from the Baltic Sea ice, two ice samples were collected from land-fast ice in a south-west Finland coastal site in February and March 2011. Bacteria were isolated from the melted sea ice samples and phages were screened from the same samples for 43 purified isolates. Plaque-producing phages were found for 15 bacterial isolates at 3 °C. Ten phage isolates were successfully plaque purified and eight of them were chosen for particle purification to analyze their morphology and structural proteins. Phage 1/32 infecting an isolate affiliated to phylum Bacteroidetes (Flavobacterium sp.) is a siphovirus and six phages infecting isolates affiliated to γ-Proteobacteria (Shewanella sp.) hosts were myoviruses. Cross titrations between the hosts showed that all studied phages are host specific. Phage solutions, host growth and phage infection were tested in different temperatures revealing phage temperature tolerance up to 45 °C, whereas phage infection was in most of the cases retarded above 15 °C. This study is the first to report isolation and cultivation of ice bacteria and cold-active phages from the Baltic Sea ice.","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"121-30"},"PeriodicalIF":2.9,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00792-013-0604-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39976910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47