Extremophiles : life under extreme conditions最新文献

Metagenomic profiling of halites from the Atacama Desert: an extreme environment with natural perchlorate does not promote high diversity of perchlorate reducing microorganisms. 阿塔卡马沙漠卤化岩的元基因组剖析：天然高氯酸盐的极端环境不会促进高氯酸盐还原微生物的高度多样性。

Extremophiles : life under extreme conditions Pub Date : 2024-04-25 DOI: 10.1007/s00792-024-01342-6

Santiago Cadena, Daniel Cerqueda-García, M. M. Uribe-Flores, Sandra I Ramírez

引用次数: 0

Biological role of the major AP (abasic site) endonuclease of an archaeon from geothermal environments. 地热环境中古细菌主要碱基核酸内切酶的生物学作用。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2022-12-02 DOI: 10.1007/s00792-022-01286-9

Rupal Jain, Dennis W Grogan

{"title":"Biological role of the major AP (abasic site) endonuclease of an archaeon from geothermal environments.","authors":"Rupal Jain, Dennis W Grogan","doi":"10.1007/s00792-022-01286-9","DOIUrl":"https://doi.org/10.1007/s00792-022-01286-9","url":null,"abstract":"Archaea and bacteria in geothermal environments are predicted to suffer DNA depurination in vivo at high rates, which raises questions regarding the biological roles of their abasic-site-repair enzymes. Gene deletion and enzymatic assay demonstrated that the saci_0015 gene of Sulfolobus acidocaldarius encodes an AP endonuclease (Apn) accounting for as much as 95% of the assayable activity in cell extracts and is not essential for viability. To identify genetic functions of this enzyme, deletion (ΔApn) strains were examined with respect to growth, spontaneous mutation, transformation by ssDNA containing an abasic site, and conjugation. Relative to its isogenic control, the ΔApn strain did not exhibit any change in growth rate or final cell density, rate or spectrum of spontaneous mutation, transformation by DNA containing an abasic site, or efficiency of DNA transfer and recombination. The apparent lack of genetic impact of removing the major AP endonuclease was unexpected and indicated that abasic sites are rarely bypassed directly by DNA polymerases in S. acidocaldarius. AP endonuclease deficiency had no obvious effect on survival of S. acidocaldarius under several test conditions, but it accelerated the death of cells at 4º C under illumination. Our results suggest that the normal level of AP endonuclease in S. acidocaldarius is well above the minimum required for growth and cell division but not for recovery from prolonged exposure to certain low-temperature conditions. This situation illustrates a biological challenge that has not been emphasized in experimental studies of extremophiles, i.e., the problem of long-term survival under \"non-extreme\" conditions.","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"1"},"PeriodicalIF":2.9,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40457844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A functionally uncharacterized type-2 malate/L-lactate dehydrogenase family protein from Thermus thermophilus HB8 catalyzes stereospecific reduction of 2-keto-3-deoxy-D-gluconate. 来自嗜热热菌HB8的2型苹果酸/ l -乳酸脱氢酶家族蛋白催化2-酮-3-脱氧-d -葡萄糖酸盐的立体特异性还原。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2022-11-23 DOI: 10.1007/s00792-022-01282-z

Tetsu Shimizu, Akira Nakamura

引用次数: 0

Rahnella sikkimica sp. nov., a novel cold-tolerant bacterium isolated from the glacier of Sikkim Himalaya with plant growth-promoting properties. 锡金拉氏菌(Rahnella sikkimica sp. nov.):一种从锡金喜马拉雅冰川中分离出来的具有促进植物生长特性的新型耐寒细菌。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2022-11-15 DOI: 10.1007/s00792-022-01283-y

Anil Kumar, Anne Le Flèche-Matéos, Rakshak Kumar, Fabienne Lomprez, Frédéric Fichenick, Dharam Singh, Patrick A D Grimont, Sanjay Kumar

{"title":"Rahnella sikkimica sp. nov., a novel cold-tolerant bacterium isolated from the glacier of Sikkim Himalaya with plant growth-promoting properties.","authors":"Anil Kumar, Anne Le Flèche-Matéos, Rakshak Kumar, Fabienne Lomprez, Frédéric Fichenick, Dharam Singh, Patrick A D Grimont, Sanjay Kumar","doi":"10.1007/s00792-022-01283-y","DOIUrl":"https://doi.org/10.1007/s00792-022-01283-y","url":null,"abstract":"The current study describes a novel species with the strain name ERMR1:05T isolated from the forefield soil of East Rathong Glacier in West Sikkim Himalaya (India). The isolate was facultatively anaerobic, gram-stain negative, non-spore-forming, rod-shaped, and oxidase negative. Whole-genome-based bacterial core gene phylogenetic analysis placed the strain in the genus Rahnella, well separated from Rouxiella spp. The digital DNA-DNA hybridisation and average nucleotide identity values between strain ERMR1:05T and other members of genus Rahnella were below the proposed thresholds for the species delineation. Based on these results, a new species, Rahnella sikkimica sp. nov., is proposed with strain ERMR1:05T (CIP 111636T, MTCC 12598T) as the type strain. The bacterium showed upregulation of cold-stress genes in cold conditions. Additionally, the genome analysis of the bacterium showed the presence of plant growth-promotion factors suggesting its role in crop improvement in cold hilly regions.","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"35"},"PeriodicalIF":2.9,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40686234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Self-assembly of Aeropyrum pernix bacilliform virus 1 (APBV1) major capsid protein and its application as building blocks for nanomaterials. 沼草杆菌状病毒1 (APBV1)主要衣壳蛋白的自组装及其在纳米材料构建中的应用

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2022-11-13 DOI: 10.1007/s00792-022-01284-x

Yuka Sumikama, Atsushi Takashima, Tomohiro Mochizuki, Haruhiko Sakuraba, Toshihisa Ohshima, Shinji Sugihara, Shin-Ichiro Suye, Takenori Satomura

引用次数: 0

Moorella sulfitireducens sp. nov., a thermophilic anaerobic bacterium isolated from a terrestrial thermal spring. 硫酸还原摩尔氏菌，一种从陆地温泉中分离出来的嗜热厌氧细菌。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2022-11-09 DOI: 10.1007/s00792-022-01285-w

Galina B Slobodkina, A Y Merkel, A A Kuchierskaya, A I Slobodkin

{"title":"Moorella sulfitireducens sp. nov., a thermophilic anaerobic bacterium isolated from a terrestrial thermal spring.","authors":"Galina B Slobodkina, A Y Merkel, A A Kuchierskaya, A I Slobodkin","doi":"10.1007/s00792-022-01285-w","DOIUrl":"https://doi.org/10.1007/s00792-022-01285-w","url":null,"abstract":"In hydrothermal ecosystems, the dissolution of sulfur dioxide in water results in the formation of sulfite, which can be used in microbial metabolism. A limited number of thermophiles have been isolated using sulfite as an electron acceptor. From a terrestrial thermal spring, Sakhalin Island, Russia, we isolated a thermophilic anaerobic bacterium (strain SLA38T). Cells of strain SLA38T were spore-forming straight rods. Growth was observed at temperatures 45-65 °C (optimum at 60 °C) and pH 5.5-9.0 (optimum at pH 6.5-7.0). The novel isolate was capable of anaerobic respiration with sulfite, thiosulfate, fumarate and perchlorate or fermentative growth. Strain SLA38T utilized glycerol, lactate, pyruvate and yeast extract. It grew lithoautotrophically on carbon monoxide with thiosulfate as electron acceptor, producing acetate. The genome size of the isolate was 2.9 Mbp and genomic DNA G + C content was 53.6 mol%. Analysis of the 16S rRNA gene sequences revealed that strain SLA38T belongs to the genus Moorella. Based on the physiological features and phylogenetic analysis, we propose to assign strain SLA38T to a new species of the genus Moorella, as Moorella sulfitireducens sp. nov. The type strain is SLA38T (= DSM 111068T = VKM B-3584T).","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"33"},"PeriodicalIF":2.9,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40453686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Halorussus vallis sp. nov., Halorussus aquaticus sp. nov., Halorussus gelatinilyticus sp. nov., Halorussus limi sp. nov., Halorussus salilacus sp. nov., Halorussus salinisoli sp. nov.: six extremely halophilic archaea isolated from solar saltern, salt lake and saline soil. vallussus sp. nov.、Halorussus aquaticus sp. nov.、Halorussus gelatinilyticus sp. nov.、Halorussus salilacus sp. nov.、Halorussus salinisoli sp. nov.:六种从太阳盐湖、盐湖和盐碱地中分离出来的极端嗜盐古细菌。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2022-10-14 DOI: 10.1007/s00792-022-01280-1

Xi-Wen Zheng, Zhang-Ping Wu, Ya-Ping Sun, Bei-Bei Wang, Jing Hou, Heng-Lin Cui

{"title":"Halorussus vallis sp. nov., Halorussus aquaticus sp. nov., Halorussus gelatinilyticus sp. nov., Halorussus limi sp. nov., Halorussus salilacus sp. nov., Halorussus salinisoli sp. nov.: six extremely halophilic archaea isolated from solar saltern, salt lake and saline soil.","authors":"Xi-Wen Zheng, Zhang-Ping Wu, Ya-Ping Sun, Bei-Bei Wang, Jing Hou, Heng-Lin Cui","doi":"10.1007/s00792-022-01280-1","DOIUrl":"https://doi.org/10.1007/s00792-022-01280-1","url":null,"abstract":"Six novel halophilic archaeal strains of XZYJT10T, XZYJ18T, XZYJT40T, XZYJT49T, YCN54T and LT46T were isolated from a solar saltern in Tibet, a salt lake in Shanxi, and a saline soil in Xinjiang, China. Sequence similarities of 16S rRNA and rpoB' genes among strains XZYJT10T, XZYJ18T, XZYJT40T, XZYJT49T, YCN54T, LT46T and current members of Halorussus were 90.6-97.8% and 87.8-96.4%, respectively. The average nucleotide identity and in silico DNA-DNA hybridization values among these six strains and current Halorussus members were in the range of 76.5-87.5% and 21.0-33.8%, respectively. These values were all below the species boundary threshold values. The phylogenomic tree based on 122 conserved archaeal protein marker genes revealed that the six novel strains formed individual distinct branches and clustered tightly with Halorussus members. Several phenotypic characteristics distinguished the six strains from current Halorussus members. Polar lipid analysis showed that the six novel strains contained phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and two to three glycolipids. Phenotypic, chemotaxonomic and phylogenetic properties showed that the six strains represented six novel species within the genus Halorussus, for which the names Halorussus vallis sp. nov., Halorussus aquaticus sp. nov., Halorussus gelatinilyticus sp. nov., Halorussus limi sp. nov., Halorussus salilacus sp. nov., and Halorussus salinisoli sp. nov. are proposed.","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"32"},"PeriodicalIF":2.9,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33511058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Electron transfer of extremophiles in bioelectrochemical systems. 生物电化学系统中极端微生物的电子转移。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2022-10-12 DOI: 10.1007/s00792-022-01279-8

Miriam Edel, Laura-Alina Philipp, Jonas Lapp, Johannes Reiner, Johannes Gescher

引用次数: 2

The catalytic and structural basis of archaeal glycerophospholipid biosynthesis. 古细菌甘油磷脂生物合成的催化和结构基础。

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2022-08-17 DOI: 10.1007/s00792-022-01277-w

Niels A W de Kok, Arnold J M Driessen

{"title":"The catalytic and structural basis of archaeal glycerophospholipid biosynthesis.","authors":"Niels A W de Kok, Arnold J M Driessen","doi":"10.1007/s00792-022-01277-w","DOIUrl":"https://doi.org/10.1007/s00792-022-01277-w","url":null,"abstract":"Archaeal glycerophospholipids are the main constituents of the cytoplasmic membrane in the archaeal domain of life and fundamentally differ in chemical composition compared to bacterial phospholipids. They consist of isoprenyl chains ether-bonded to glycerol-1-phosphate. In contrast, bacterial glycerophospholipids are composed of fatty acyl chains ester-bonded to glycerol-3-phosphate. This largely domain-distinguishing feature has been termed the \"lipid-divide\". The chemical composition of archaeal membranes contributes to the ability of archaea to survive and thrive in extreme environments. However, ether-bonded glycerophospholipids are not only limited to extremophiles and found also in mesophilic archaea. Resolving the structural basis of glycerophospholipid biosynthesis is a key objective to provide insights in the early evolution of membrane formation and to deepen our understanding of the molecular basis of extremophilicity. Many of the glycerophospholipid enzymes are either integral membrane proteins or membrane-associated, and hence are intrinsically difficult to study structurally. However, in recent years, the crystal structures of several key enzymes have been solved, while unresolved enzymatic steps in the archaeal glycerophospholipid biosynthetic pathway have been clarified providing further insights in the lipid-divide and the evolution of early life.","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"29"},"PeriodicalIF":2.9,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40618200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Role of carnitine in adaptation of Chromohalobacter salexigens DSM 3043 and its mutants to osmotic and temperature stress in defined medium. 肉碱在salexigens色盐杆菌DSM 3043及其突变体适应特定介质渗透和温度胁迫中的作用

IF 2.9

Extremophiles : life under extreme conditions Pub Date : 2022-08-14 DOI: 10.1007/s00792-022-01276-x

Xiang-Lin Meng, Xia Gao, Yuan-Ming Si, Li-Li Xu, Li-Zhong Guo, Wei-Dong Lu

{"title":"Role of carnitine in adaptation of Chromohalobacter salexigens DSM 3043 and its mutants to osmotic and temperature stress in defined medium.","authors":"Xiang-Lin Meng, Xia Gao, Yuan-Ming Si, Li-Li Xu, Li-Zhong Guo, Wei-Dong Lu","doi":"10.1007/s00792-022-01276-x","DOIUrl":"https://doi.org/10.1007/s00792-022-01276-x","url":null,"abstract":"L-Carnitine is widespread in nature, but little information is available on its metabolism and physiological functions in moderate halophiles. In this study, we found that Chromohalobacter salexigens DSM 3043 could utilize carnitine not only as a nutrient, but also as an osmolyte. When grown at 37 °C under salt-stress conditions, the strain utilized carnitine as an osmoprotectant by enzymatically converting it into GB. When grown at low and high temperature, both carnitine and its metabolic intermediate GB were simultaneously accumulated intracellularly, serving as cryoprotectants and thermoprotectants. The genes (csal_3172, csal_3173, and csal_3174) which were predicted to participate in L-carnitine degradation to GB were deleted to construct the corresponding mutants. The effects of salinity and temperature on the growth rates and cytoplasmic solute pools of the C. salexigens wild-type and mutant strains were investigated. 13C-NMR analysis revealed that GB was still detected in the Δcsal_3172Δcsal_3173Δcsal_3174 mutant grown in a defined medium with added DL-carnitine, but not with L-carnitine, indicating that an unidentified D-carnitine degradation pathway exists in C. salexigens. Taken together, the data presented in this study expand our knowledge on carnitine metabolism and its physiological functions in C. salexigens exposed to single or multiple environmental abiotic stress.","PeriodicalId":288153,"journal":{"name":"Extremophiles : life under extreme conditions","volume":" ","pages":"28"},"PeriodicalIF":2.9,"publicationDate":"2022-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40611225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1