T. Kawahara, Tatsuhiko Hirano, Lin Feng, H. Uvet, M. Hagiwara, Y. Yamanishi, F. Arai
{"title":"High-speed single cell dispensing system","authors":"T. Kawahara, Tatsuhiko Hirano, Lin Feng, H. Uvet, M. Hagiwara, Y. Yamanishi, F. Arai","doi":"10.1109/MHS.2011.6102235","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102235","url":null,"abstract":"In this paper, we discuss the single cell dispensing system to increase both the speed and the success rate of single cell dispensing. Two pairs of capacitance sensors are placed in a biochip to detect the flow velocity of cells, and the air pressure is applied to eject cells by synchronizing the timing. Then, the system theoretically has a capability to eject 3 cells/sec with maximum flow velocity is 10 mm/sec. Finally, we succeeded in automatic dispensing of a single polystyrene bead (=100 µm) from a biochip to culture well atmosphere using developed cell ejection system with the success rate of 50 %. Furthermore, we also succeeded in single swine oocyte dispensing by developed system.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"89 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123171047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evolutionary approach of reward function for reinforcement learning using genetic programming","authors":"Shota Sumino, Atsuko Mutoh, Shohei Kato","doi":"10.1109/MHS.2011.6102214","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102214","url":null,"abstract":"In recent year, reinforcement learning, which acquires a behavior of robots has been drawing attention. A suitable behavior is autonomously acquired by using this system. Robots learn the suitable behavior by iterating action and receiving the evaluated value of that action. The evaluated value is calculated by reward function. In general reinforcement learning, we acquire a suitable behavior by setting the suitable reward function for each problem. However in previous research of reinforcement learning, most reward functions are constructed based on human's heuristics. To construct reward functions, trial-and-error is needed, and it imposes an enormous drain on humans. Therefore we propose an approach, which automatically generate reward functions, using Genetic Programming. In this approach, we create a method evaluating reward functions. Reward functions are generated by Genetic Programming, and are evaluated by evaluating method. A suitable reward function is generated by evolution of these reward functions. In this paper, we conducted an experiment to confirm the effectiveness of proposed method. In the experiment, we generate a suitable reward function of a problem, which a route searching problem in a tile-world. Through the experiment, we confirm that the proposed approach can generate a suitable reward function, and the generated reward function can acquire a more suitable behavior in comparison with a reward function by constructed based on human's heuristics.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123380722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Jatmiko, P. Mursanto, A. Febrian, M. Fajar, W. T. Anggoro, R. S. Rambe, M. I. Tawakal, F. Jovan, S. M.Eka
{"title":"Arrhythmia classification from wavelet feature on FGPA","authors":"W. Jatmiko, P. Mursanto, A. Febrian, M. Fajar, W. T. Anggoro, R. S. Rambe, M. I. Tawakal, F. Jovan, S. M.Eka","doi":"10.1109/MHS.2011.6102207","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102207","url":null,"abstract":"Arrhythmia is a condition when heart beats are not beating properly, either in rhythm or in intensity. Sometimes arrhythmia problems could make patients in dangerous condition due to their sortie. However, good classification and diagnostic in arrhythmia will help many lives from fatal menace. Many different diagnostics and classifications have been conducted recently by using neural network as their classifier, both in simulation and real hardware implementation. Nevertheless, the products as an arrhythmia classifier are not small enough for daily use. Our previous research [3] succeeded making a simulation for heart beats classifier on neural network. In this research, we tried to implement it on a prototype small arrhythmia classifier on FPGA using Spartan 3AN development board.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125232599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mun'delanji C. Vestergaard, Tsuyoshi Yoda, T. Hamada, Y. Akazawa, Y. Yoshida, M. Takagi
{"title":"Thermo-responsiveness of auto-oxidized cholesterol-containing lipid membranes, observed in real-time","authors":"Mun'delanji C. Vestergaard, Tsuyoshi Yoda, T. Hamada, Y. Akazawa, Y. Yoshida, M. Takagi","doi":"10.1109/MHS.2011.6102230","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102230","url":null,"abstract":"There are increasing many reports linking oxidation of the membrane to various diseases such as atherosclerosis. Using cell-sized (giant) unilamellar lipid vesicles, we studied the effect of exposing the lipid vesicles to an oxidative stress environment. We found that the vesicles underwent fluctuations, and some had morphological transformation. We characterized the main products of cholesterol oxidation as 7β-hydroxycholesterol (7β) and 7-ketocholesterol (7keto). After, we prepared lipid membrane containing the above oxy-cholesterols and investigated the influence of temperature on membrane dynamics. We found that (i) 7b and 7keto rendered vesicles more responsive to temperature changes, (ii) 7keto imparted greater thermo-induced membrane dynamics than 7β, (iii) 7b and 7keto vesicles synergistically, were more thermo-responsive than the individual oxysterols, (iv) the thermo-responsiveness of 7keto-containing vesicles was equivalent to that of 25 hydroxycholesterol (25OH)-containing vesicles, In addition, we carried out langmuir monolayer experiments using the membrane systems above. The results show an increase in molecular area with increase in temperature. and correlated well with the membrane dynamics upon temperature change. These results are important to understanding mechanisms of membrane dynamics upon oxidative stress. The interplay between cholesterol oxidation and temperature provides new ideas for development of oxidative stimulus-dependent therapeutics, including thermo therapy.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130097700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Tadakuma, R. Tadakuma, M. Higashimori, M. Kaneko
{"title":"Finger mechanism equipped omnidirectional driving roller","authors":"K. Tadakuma, R. Tadakuma, M. Higashimori, M. Kaneko","doi":"10.1109/MHS.2011.6102236","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102236","url":null,"abstract":"The goal of this article is to give you basic instructions for preparing papers for the 2011 International Symposium on Micro-NanoMechatronics and Human Science. Please prepare your final manuscripts following the guidelines described in this article.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126968899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lin Feng, H. Uvet, T. Kawahara, M. Hagiwara, Y. Yamanishi, F. Arai
{"title":"On-chip high-speed and on-demand single microbeads loading","authors":"Lin Feng, H. Uvet, T. Kawahara, M. Hagiwara, Y. Yamanishi, F. Arai","doi":"10.1109/MHS.2011.6102195","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102195","url":null,"abstract":"This paper presents on-demand microbeads delivery system in a microfluidic chip by magnetically driven microtool (MMT). The four of horizontally arranged permanent magnets and the piezoelectric vibrator are used to actuate the Ni based microtool precisely in the chip. The MMT injects the beads mechanically to flow and then into ejection operation part. We succeeded in transferring microbeads one by one with the required pace to the next process in order to achieve stable dispensing microbeads out from the microfluidic chip to incubation environment. We succeeded in demonstration of the delivery and ejection of polystyrene beads (100 µm) with the flow velocity of from 0.02 ml/h to 0.04 ml/h and MMT frequency from 1 Hz to 6 Hz to adjust the pitch of each micro-bead. The spacing interval of microbeads could be mainly adjusted by changing of MMT frequency and the flow velocity of output stream. The proposed system shows advantages of high-speed, high success ratio and disposable of microfluidic chip having MMT.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115819907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuyang Lee, R. Sudo, Tomoya Komatsu, N. Miki, T. Mitaka, M. Ikeda, K. Tanishita
{"title":"Microfluidic Hydrostatic Deposition Patterning for a confined hepatocyte-biliary epithelial cell co-culture system","authors":"Yuyang Lee, R. Sudo, Tomoya Komatsu, N. Miki, T. Mitaka, M. Ikeda, K. Tanishita","doi":"10.1109/MHS.2011.6102148","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102148","url":null,"abstract":"Reconstruction of hepato-biliary networks is very important to maintaining liver organoids in vitro, since the accumulation of bile has been proven to be toxic to hepatocytes, especially in long-term culture. We have developed culture methods for reconstructing bile canaliculi (BC) and bile ducts (BDs) formed by small hepatocytes (SHs) which are hepatic progenitor cells, and biliary epithelial cells (BECs), respectively. To study the mechanism of biliary excretion, we need to establish a co-culture system for SHs and BECs. A limitation of conventional culture methods in investigating the mechanisms of hepato-biliary connections is the difficulty to observe the dynamic interactions between SHs and BECs in culture. In addition, patterned constructs do not form in conventional cultures due to the lack of organization of cells. A system that allows more complex dynamics between SHs and BECs to occur is needed in order to investigate the interactions between these cell types. Here, we present an approach called Microfluidic Hydrostatic Deposition Patterning (MHDP) that patterns SH and BEC colonies with defined shape and position, which promotes productive interactions between the cells and eventually induce the formation of heterogeneous tissues integration which is thought to be a prototype of complete biliary networks.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122918631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Sugimoto, Y. Hadate, K. Yagi, M. Kubo, Naohiro Yazu, T. Inaba
{"title":"Synthesis and material properties of magnetic fluid coated with pegylated chondroitin sulfate","authors":"S. Sugimoto, Y. Hadate, K. Yagi, M. Kubo, Naohiro Yazu, T. Inaba","doi":"10.1109/MHS.2011.6102184","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102184","url":null,"abstract":"We tried to fabricate a magnetic fluid coated with chondroitin sulfate having “stealth” function by PEGylation of chondroitin sulfate (CS).","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126773113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Communicative humanoid robot control system reflecting human body movement","authors":"Akinori Wakabayashi, Satona Motomura, Shohei Kato","doi":"10.1109/MHS.2011.6102172","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102172","url":null,"abstract":"Recently, humanoid robot has been focused and researched as the medium of remote communication, because it enables us to transmit nonverbal information and share the physical space. However, in the existing studies about robot control system by human body movement, the detailed structure information of the robot (e.g., degrees of freedom, the range of motion and forms) must be examined in order to calculate inverse kinematics. In our previous work, we proposed Associative Motion Generation (AMG) as humanoid robot motion generation method which does not need detailed structure information of the robot. In this paper, we propose the remote physical communication system using the small humanoid robot that enables us intuitively to control the robot. And by using AMG, the proposed system can be applied to any robots, since general wide-spread of communication using humanoid robot are expected. Through the communication experiment, we confirmed the effectiveness of the proposed system.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121021686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qing-yun Liu, K. Fukuzawa, Y. Kajihara, Hedong Zhang, S. Itoh
{"title":"Vertical-objective-based ellipsometric microscope for backside illuminated real-time visualization of nm-thick lubricant films","authors":"Qing-yun Liu, K. Fukuzawa, Y. Kajihara, Hedong Zhang, S. Itoh","doi":"10.1109/MHS.2011.6102157","DOIUrl":"https://doi.org/10.1109/MHS.2011.6102157","url":null,"abstract":"The vertical-objective-based ellipsometric microscope (VEM) is presented which can be used for real-time visualization of nm-thick lubricant films with high lateral resolution. The performance of hard disk drives (HDDs) depends on the dynamics of the nm-thick liquid lubricant films due to decrease of head-disk gap to 2 to 3 nm. The contact or near-contact head disk interface is needed to increase the recording intensity. However, meniscus formation or lubricant pick-up will occur. Therefore, real-time visualization of shearing nm-thick liquid lubricant films plays an important role in the analysis of these phenomena. However, the current VEM can not provide this contact or near-contact situation due to the short working distance (WD) of the high magnification objective lens. In this report, we propose a backside illuminated VEM, whose illumination system and objective lenses are set on the opposite of the sliding probe to solve this problem, and the feasibility of the method is discussed.","PeriodicalId":286457,"journal":{"name":"2011 International Symposium on Micro-NanoMechatronics and Human Science","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129207970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}