Biomedical Chemistry: Research and Methods最新文献_第5页

Identification of Androgen Deficiency in Infertility and Reduced Ovarian Reserve Based on HPLCMS/MS and IHLA Measurements 基于HPLCMS/MS和IHLA测定的不孕症雄激素缺乏和卵巢储备减少的鉴定

Biomedical Chemistry: Research and Methods Pub Date : 1900-01-01 DOI: 10.18097/bmcrm00182

S. Kindysheva, A. Gavisova, M. A. Shevtsova, L.T. Tskhovrebova, D. A. Biryukova, N. Starodubtseva, T.Yu. Ivanec, V. Frankevich

引用次数: 0

SPR Biosensor Based High-Throughput Screening of Low Molecular Weight Compounds for Interaction with Candida krusei CYP51 基于SPR生物传感器的高通量筛选与克鲁假丝酵母CYP51相互作用的低分子量化合物

Biomedical Chemistry: Research and Methods Pub Date : 1900-01-01 DOI: 10.18097/bmcrm00183

L. Kaluzhskiy, T. V. Tsybruk, E. Yablokov, O. Gnedenko, E. Zelepuga, A. Kicha, E. Kozlovskaya, N. V. Ivanchina, A. Gilep, A. S. Ivanov

引用次数: 1

Evaluation of the Collateral Activity Of CRISPR/Cas13a-Ribonuclease on the Agilent 2100 Bioanalyzer CRISPR/ cas13a核糖核酸酶在Agilent 2100生物分析仪上的侧支活性评价

Biomedical Chemistry: Research and Methods Pub Date : 1900-01-01 DOI: 10.18097/bmcrm00169

L. Kurbatov, S. Radko, S. Khmeleva, O. Timoshenko, A. Lisitsa

引用次数: 0

Standardization of Recombinant CRISPR/Cas13a-nuclease Preparations by Using RNase A of Known Activity 利用已知活性RNase A对重组CRISPR/ cas13a核酸酶制剂进行标准化

Biomedical Chemistry: Research and Methods Pub Date : 1900-01-01 DOI: 10.18097/bmcrm00177

L. Kurbatov, S. Radko, S. Khmeleva, O. Timoshenko, A. Lisitsa

引用次数: 1

Proteoform Identification in 2D Electrophoresis Maps by Using Isoelectric Point Prediction 基于等电点预测的二维电泳图谱中蛋白质形态识别

Biomedical Chemistry: Research and Methods Pub Date : 1900-01-01 DOI: 10.18097/bmcrm00191

A. Rybina

引用次数: 0

Construction and Expression of the Chimeric Human Renalase Gene Encoding the N-Terminal Signal Sequence of the Secretory Protein Prolactin 编码泌乳蛋白n端信号序列的人Renalase嵌合基因的构建与表达

Biomedical Chemistry: Research and Methods Pub Date : 1900-01-01 DOI: 10.18097/bmcrm00175

V. Fedchenko, A. Kaloshin, A. Veselovsky, A. E. Medvedev

{"title":"Construction and Expression of the Chimeric Human Renalase Gene Encoding the N-Terminal Signal Sequence of the Secretory Protein Prolactin","authors":"V. Fedchenko, A. Kaloshin, A. Veselovsky, A. E. Medvedev","doi":"10.18097/bmcrm00175","DOIUrl":"https://doi.org/10.18097/bmcrm00175","url":null,"abstract":"Renalase (RNLS) is a protein that performs various protective functions both inside and outside cells. Intracellular RNLS is a FAD-dependent oxidoreductase (EC 1.6.3.5). Extracellular RNLS lacking an N-terminal peptide does not interact with FAD and exhibits various protective effects on the cell through interaction with receptor proteins. The mechanisms and factors responsible for RNLS transport out of the cell are not fully understood. It is well known that the signal sequence plays a key role in the classical mechanism of protein transport outside cells. One of the approaches to study the secretion of RNLS from the cell can be the creation of chimeric forms of the protein with a modified N-terminal amino acid signal sequence. Bioinformatics analysis showed that the signal sequence of the prolactin gene (PRL), connected to the template sequence of the RNLS gene, gave the classic signal characteristic of secretory proteins. On this basis, this paper describes: (i) a method for constructing the human RNLS gene in which the N-terminal sequence encoded by the RNLS gene was replaced by the N-terminal sequence encoded by the PRL gene; (ii) expression of this chimeric genetic construct.","PeriodicalId":286037,"journal":{"name":"Biomedical Chemistry: Research and Methods","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125589197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Geldanamycin Binding on the Thr90 Phosphorylation Site of Hsp90 格尔达霉素结合对热休克蛋白90 Thr90磷酸化位点的影响

Biomedical Chemistry: Research and Methods Pub Date : 1900-01-01 DOI: 10.18097/bmcrm00145

K. Shcherbakov, D. S. Shcherbinin, A. Veselovsky

{"title":"Effect of Geldanamycin Binding on the Thr90 Phosphorylation Site of Hsp90","authors":"K. Shcherbakov, D. S. Shcherbinin, A. Veselovsky","doi":"10.18097/bmcrm00145","DOIUrl":"https://doi.org/10.18097/bmcrm00145","url":null,"abstract":"Prostate cancer is hormone-dependent and the androgen receptor (AR) is involved in its development. AR is a transcription factor that is activated by ligand binding, result in its translocation into the nucleus, where it initiates gene transcription. In an inactive state in cytoplasm AR exists as a complex with heat shock protein 90 (HSP90) and some other proteins. When the agonist binds, a conformational change in AR occurs, resulting in HSP90 and other chaperones dissociating. Recently it has been shown that for the dissociation of the HSP90-AR complex and the translocation of the latter into the nucleus, phosphorylation of the Thr-90 residue of the N-terminal domain of HSP90 is necessary. In this work, the effect of the HSP90 inhibitor, geldanamycin, interacting with the ATP-binding site, on the Thr90 phosphorylation site was investigated by molecular modeling methods. It has been shown that inhibitor binding slightly affects the size and mobility of cavity around Thr90. It is suggested that inhibitor binding to HSP90 does not result in changing the protein structure and does not influence on protein phosphorylation, and partially explains low effectiveness of such type of drugs in the therapy of prostate cancer.","PeriodicalId":286037,"journal":{"name":"Biomedical Chemistry: Research and Methods","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131875587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Superoxide Generation by Nicotinamide Coenzymes 烟酰胺辅酶产生超氧化物

Biomedical Chemistry: Research and Methods Pub Date : 1900-01-01 DOI: 10.18097/bmcrm00188

T. V. Sirota

引用次数: 0

Methods for the Preparation of Blood Serum Samples in the Assessment of the Pharmacokinetics of Protein Drugs by the Example of Viscumin 蛋白类药物药动学评价血清样品制备方法(以粘胶素为例)

Biomedical Chemistry: Research and Methods Pub Date : 1900-01-01 DOI: 10.18097/bmcrm00152

N. Yudina, Ya.N. Barashkova, O. Vladimirova, V. Myasnikov

{"title":"Methods for the Preparation of Blood Serum Samples in the Assessment of the Pharmacokinetics of Protein Drugs by the Example of Viscumin","authors":"N. Yudina, Ya.N. Barashkova, O. Vladimirova, V. Myasnikov","doi":"10.18097/bmcrm00152","DOIUrl":"https://doi.org/10.18097/bmcrm00152","url":null,"abstract":"A comparative evaluation of methods for preparing blood serum samples for determination of the lectin content of the mistletoe Viscum Album, viscumin, by high-performance liquid chromatography in combination with high-resolution mass-spectrometry detection has been carried out. Based on the results of the analysis of the lectin hydrolyzate, a specific peptide fragment with m/z 791.388 suitable for subsequent quantitative determination was selected. Isolation of viscumin from serum components was carried out without the use of specific antibodies. The study used methods employing various spin columns and the method of protein precipitation with 1% TCA (trichloroacetic acid) in IPA (isopropyl alcohol). Testing the methods of depletion of blood serum proteins was based on the determination of the degree of extraction of lectin from model serum samples. As a result of our research, we have developed a technique for the quantitative analysis of viscumin in blood serum by the most efficient method of sample preparation using the ProteoMiner spin columns. The technique allows to determine the protein concentration up to 5·10-4 mg/ml with the extraction degree (2.7 ± 0.6) %.","PeriodicalId":286037,"journal":{"name":"Biomedical Chemistry: Research and Methods","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128622892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Algorithms for Calculation of Parameters of Electrochemical Biosensor 电化学生物传感器参数的计算算法

Biomedical Chemistry: Research and Methods Pub Date : 1900-01-01 DOI: 10.18097/bmcrm00178

V. Shumyantseva, V. Pronina

引用次数: 0