Identification of Androgen Deficiency in Infertility and Reduced Ovarian Reserve Based on HPLCMS/MS and IHLA Measurements

Abstract

The androgen deficiency and associated states represent is an important problem that affects the quality of women live. The most widely the androgen influence has been studied in the reproductive period in relation to polycystic ovary syndrome; however about laboratory methods to determine the impact of androgen deficiency and its clinical manifestation in the case of young women with a reduced ovarian reserve and with infertility are still actively discussed. Clinical medicine still needs generally approved markers of androgen deficiency states and its lower reference values. In this work we illustrate the perspective of measurements of steroid hormones panel to verify the diagnosis on the basis of high performance liquid chromatography with tandem mass spectrometry and immunochemical methods.