Journal of Biotechnology & Bioresearch最新文献

{"title":"The Molecular Approach behind Biotechnological Common Problems","authors":"J. A. De Los Santos","doi":"10.31031/jbb.2019.02.000535","DOIUrl":"https://doi.org/10.31031/jbb.2019.02.000535","url":null,"abstract":"A fascinating concept in biology, in my personal opinion, is how cells are able to make new cells expressing the information from genes to proteins all over the earth. From unicellular organisms, viruses, mycoplasma, protozoa, cancer cells, neurons to the most complex biological organisms and complex ecosystems, all of them rely on the gene expression. Darwin in his famous book “On the Origin of Species” in 1859, states that the species of organisms live and evolve through the natural selection processes. In 1976 Richard Dawkins states in his book “The Selfish Gene”, that genes are the central piece in the way natural selection operates [1]. In this paper, I am going to explore the relationship between genes under the “selfish” rule and how these can inspire new technologies.","PeriodicalId":274001,"journal":{"name":"Journal of Biotechnology & Bioresearch","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131026030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Assessment of the Biofield Energy Healing Based Test Formulation on Human Organ Health Specific Biomarkers In Vitro Assays\"","authors":"E. M. Meagher, M. Trivedi, A. Branton, Dahryn Trivedi, G. Nayak, M. Gangwar, S. Jana","doi":"10.31031/jbb.2019.02.000529","DOIUrl":"https://doi.org/10.31031/jbb.2019.02.000529","url":null,"abstract":"Herbal based test formulations have been used in most of the healthcare settings since time immemorial. The present experimental cell line study was designed to evaluate the impact of the Biofield Energy Treatment on test formulation and different cell line mediums related with vital organs functioning. Cell lines that were specific to different organ systems were used in the study using standard protocols. The Test Item (TI) and specific cell line media (Med) was divided into two parts; one untreated (UT-TI) and other part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Eileen Mary Meagher, USA and were labeled as the Biofield Energy Treated (BT) test formulation/media. MTT assay was used for cell viability assay, and the results showed that the test item was found non-toxic. Cytoprotective action of the test formulation showed a significant maximum restoration of cell viability by 56.8% (at 63.75µg/mL), 57.5% (at 63.75µg/mL), and 124.8% (at 1µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups respectively, as compared to the untreated test group in Human Cardiac Fibroblasts cells (HCF) cells, while 83.2% (at 63.75µg/mL), 93.8% (at 63.75µg/mL), and 20.6% (at 10µg/mL) improved cellular protection of human Hepatoma cells (HepG2) cells in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups respectively, as compared to the untreated test group. In addition, cytoprotective activity in adenocarcinoma human alveolar basal epithelial cells (A549) showed improved cell viability by 11.3% (at 25.5µg/mL), 82.7% (at 63.75µg/mL), and 113.9% (at 25.5µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups respectively, as compared to the untreated test group. MG-63 cells were used for estimation of ALP activity, which was highly increased by 59.8% (at 0.1µg/mL), 269.7% (at 0.1µg/mL), and 105.7% (at 0.1µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups respectively, as compared to the untreated test group. Besides, Ishikawa cells showed maximum increased ALP activity by 94.8% at 10µg/mL in the BT-Med+UT-TI group as compared to the untreated group. The maximum percent cellular protection of HCF (heart) cells (decreased of LDH activity) was significantly increased by 48.8% (at 1µg/mL), 62.9% (at 10µg/mL), and 103% (at 1µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI group, and BT-Med+BT-TI groups respectively, as compared to the untreated test group. Alanine Amino Transferase (ALT) in terms of percent protection of HepG2 (liver) cells (decreased of ALT activity) was reported at 63.75µg/mL by 74.9% and 84.9% in the BT-Med+UT-TI and BT-Med+BT-TI groups respectively, as compared to the untreated test group. Cellular protection of A549 (lungs) cells (increased of SOD activity) in terms of percentage was increased by 21.7% (at 25.5µg/mL), 83.2% (at 0.1µg/mL), and 40% (at 25.5µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups, respectively as compared to untreated group. Serotonin l","PeriodicalId":274001,"journal":{"name":"Journal of Biotechnology & Bioresearch","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132027845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioremediation of Agro-Wastewater of Poultry in a Microbial Fuel Cell","authors":"L. A. Obasi, O. Onukwuli","doi":"10.31031/jbb.2019.02.000534","DOIUrl":"https://doi.org/10.31031/jbb.2019.02.000534","url":null,"abstract":"Besides, the search for alternative sources of energy has increased due to current prediction of the global energy depletion [2]. There is therefore a clarion call for sustenance of the worldwide energy demand and a balance of the effect of energy demand chain operation on the environment. The challenge, therefore, is that fossil fuel as the hub of world energy supply is exhaustible implying that several centralized power plants probably may become redundant due to lack of fuel. Hence the focus is now on the quest for strategic solution designed this growing world energy demand [3].","PeriodicalId":274001,"journal":{"name":"Journal of Biotechnology & Bioresearch","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130847053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein Characteristic for New Beginners","authors":"L. Gam","doi":"10.31031/jbb.2019.02.000533","DOIUrl":"https://doi.org/10.31031/jbb.2019.02.000533","url":null,"abstract":"One of the branches of biotechnology is proteomics. It is an interesting field as proteins, unlike DNA which made up of combination of 4 nucleotides, is made up of combination of 22 amino acids, where the sequence of the amino acids gives uniqueness to each protein. In addition, the structure of the protein such as primary, secondary, tertiary and the folding of protein into quaternary making the protein different although they may have similar amino acid sequence. Furthermore, a functional protein may be consisting of more than one polypeptide. Each of the amino acid has different characteristic therefore the combination of the amino acids making protein to have different degrees of polarity. In addition, the post translation modification of protein, such as glycosylation, lipid components and metal ions making the protein to function differently according to their activity. All this made handling of protein difficult and if one is not careful, it may cause the problem in reproducibility of protein based experimental work.","PeriodicalId":274001,"journal":{"name":"Journal of Biotechnology & Bioresearch","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131192003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kombucha and Kefir are Foods of the 21st Century: A Opinion","authors":"K. Magalhães-Guedes","doi":"10.31031/JBB.2019.02.000532","DOIUrl":"https://doi.org/10.31031/JBB.2019.02.000532","url":null,"abstract":"Kefir is a fermented milk product having slight acidic taste, carbonation, and aroma good. The kefir beverage contains a specific mixture of microorganisms (lactic bacteria, acetic bacteria e yeast) that reside in symbiotic manner in a kefiran polysaccharide matrix. Kefir grains (Figure 1) can be characterized as small cauliflower florets, having a length of 10-40mm, irregularly shaped, white to yellowish in color, having firm texture and slimy appearance. The kefir microorganisms have a capacity to produce organic acids, antibiotics, and numerous types of antimicrobials; these substances have a lethal effect on pathogenic microorganisms of the body. Kefiran is an exopolysachhardie component of kefir grain that has significant importance in human health and nutrition [1,2].","PeriodicalId":274001,"journal":{"name":"Journal of Biotechnology & Bioresearch","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125910362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rare Case of Sezary Syndrome Cutaneous T-Cell Lymphoma with Complete Response","authors":"H. Im, I. Bondarenko, V. Zavizion, Maltseva Liebov, GElhaj Mohamad, Hurtovyi Vadym, Hojouj Tamara, Tereshchenko Pavlo, Tereshchenko Sergii","doi":"10.31031/jbb.2019.02.000531","DOIUrl":"https://doi.org/10.31031/jbb.2019.02.000531","url":null,"abstract":"","PeriodicalId":274001,"journal":{"name":"Journal of Biotechnology & Bioresearch","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115616896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Disagreement with The Idea of Decarbonizing Economy as Means of Stopping Climate Change and Some Proposals to Perform This Task Under the Viewpoint Take the Available\"","authors":"M. Ringpfeil","doi":"10.31031/jbb.2019.02.000528","DOIUrl":"https://doi.org/10.31031/jbb.2019.02.000528","url":null,"abstract":"","PeriodicalId":274001,"journal":{"name":"Journal of Biotechnology & Bioresearch","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126076513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acrylamide-A Harmful Chemical Present in Food Entities","authors":"N. Zahra, Bukhtawar Tariq, Rabea Yaseen, I. Kalim, M. K. Saeed, Muhammad Ahsan Ashraf, H. Amin","doi":"10.31031/jbb.2019.02.000527","DOIUrl":"https://doi.org/10.31031/jbb.2019.02.000527","url":null,"abstract":"Acrylamide presence in food has become a serious issue as it leads to cancer formation. Acrylamide is present in many of daily use products and is potentially hazardous for public health as well as environment. Acrylamide a simple organic compound that is responsible for causing cancer, neuro-degenerative diseases as well as other reproductive issues. Acrylamide is present in most of our food products and get worse with the cooking or heating process. It accounts for major health concerns because of its high-level consumption especially by young generation, which is fond of processed and baked food now-a-days. The conversion of acrylamide into carcinogenic agent is by the reaction called as Maillard reaction, which often leads to the browning or change in the food. time exposure to a substance containing acrylamide can be source of cancer or other disorders. The main purpose of this review is to summarize the methods for the detection of acrylamide, its major health concerns, its mitigation as well as its permissible limits along with its formation.","PeriodicalId":274001,"journal":{"name":"Journal of Biotechnology & Bioresearch","volume":"131 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125052439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-Based Materials for Wound Healing and Tissue Engineering: An Overview on their Properties and Applications","authors":"M. Kasaai","doi":"10.31031/JBB.2019.02.000526","DOIUrl":"https://doi.org/10.31031/JBB.2019.02.000526","url":null,"abstract":"In this study, an effort has been made to provide a global view from some available literature information on properties and applications of chitosans for wound healing and tissue engineering. Renewable resources, Environmentally friendly, Biodegradability, Biocompatibility, Non-toxicity and Anti-microbial properties make chitosans as the most promising and interesting materials for medicine and health care applications. Chitosans and their some derivatives also exhibit film and fibre forming properties. Chitosans with Hydrophilic/ Hydrophobic nature provide a variety of surface functional groups to bind with bioactive compounds, and target organs of the human body, and consequently result in an increase in their stability. Hydrophilic/ Hydrophilic characters of the chitosans would yield in various possibilities and finally open several opportunities for various applications in medicine and health care sectors. Chitosan is a unique Biotechnological/ Biomaterial choice for wound healing and tissue engineering.","PeriodicalId":274001,"journal":{"name":"Journal of Biotechnology & Bioresearch","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127062146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Systemic Inflammation in Neurodegenerative Diseases","authors":"Beatrice D and Senejani Ag","doi":"10.31031/jbb.2019.01.000525","DOIUrl":"https://doi.org/10.31031/jbb.2019.01.000525","url":null,"abstract":"The role of systemic inflammation in neurodegeneration is complex. It involves interplay between several pro-inflammatory and anti-inflammatory factors, DNA damage and a cycle of neurotoxic inflammation and tissue degradation. Neuroinflammation can trigger neurodegeneration. Though suppressing system ic inflammation might be assumed to be neuroprotective against neurodegeneration, inhibiting certain features of the systemic immune response may cause damage.","PeriodicalId":274001,"journal":{"name":"Journal of Biotechnology & Bioresearch","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134093446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}