ACS Synthetic Biology最新文献_第6页

Identification of Biosynthetic Precursors and Optimization of 7-Hydroxytropolone Bioproduction by Pseudomonas sp. PA14H7. 假单胞菌PA14H7生物合成前体的鉴定及生产7-羟基曲罗酮的优化

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18 Epub Date: 2025-06-12 DOI: 10.1021/acssynbio.4c00817

Euphrasie Munier-Lépinay, Coline Amaro-Lauer, Denis Faure, Anthony Quéro, David Mathiron, Mounia Khelifa, Sylvain Laclef, Serge Pilard

{"title":"Identification of Biosynthetic Precursors and Optimization of 7-Hydroxytropolone Bioproduction by Pseudomonas sp. PA14H7.","authors":"Euphrasie Munier-Lépinay, Coline Amaro-Lauer, Denis Faure, Anthony Quéro, David Mathiron, Mounia Khelifa, Sylvain Laclef, Serge Pilard","doi":"10.1021/acssynbio.4c00817","DOIUrl":"10.1021/acssynbio.4c00817","url":null,"abstract":"Cell-free supernatant of the strain Pseudomonas sp. PA14H7 has previously shown interesting activity against Soft Rot Pectobacteriaceae (SRP), the bacterial pathogen responsible for blackleg and soft rot diseases in potatoes. A deeper understanding of its mode of action is essential to optimize its use as a biocontrol agent. We previously reported that Pseudomonas sp. PA14H7 produces a specialized metabolite, the 7-hydroxytropolone (7-HT), which acts as an iron chelator, limiting the growth of SRP. In this study, we have constructed a Δhts10 deletion mutant of Pseudomonas sp. PA14H7, encoding a putative acyl-CoA dehydrogenase corresponding to the ortholog of orf10 in Pseudomonas donghuensis. We demonstrated that this mutant was deficient in 7-HT biosynthesis, confirming that this molecule is the metabolite responsible for the antagonist activity. After finding a minimum culture medium (MK) allowing the Pseudomonas sp. PA14H7 growth without 7-HT production, we investigated the biosynthetic pathway of this metabolite. We identified phenylalanine and phenylacetic acid as 7-HT precursors and demonstrated that the addition of 150 mg/L of phenylalanine to the MK medium enhanced the 7-HT bioproduction by Pseudomonas sp. PA14H7 up to 30 mg/L. These findings provide new insights into the biosynthesis and regulation of 7-HT, paving the way for the use of Pseudomonas sp. PA14H7 as a biocontrol agent.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2525-2536"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Review of l-Theanine Production in Escherichia coli: The Recent Progress, Metabolic Engineering Strategies, and Future Prospects. 大肠杆菌生产l-茶氨酸的综述：最新进展、代谢工程策略和未来展望

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18 Epub Date: 2025-07-04 DOI: 10.1021/acssynbio.5c00291

Yuhuan Zhang, Mengzhen Jia, Junyao Huang, Zhicheng Fu, Jiarong Liu, Yanlin Kang, Hui Yang, Bin Zhang

{"title":"A Comprehensive Review of l-Theanine Production in Escherichia coli: The Recent Progress, Metabolic Engineering Strategies, and Future Prospects.","authors":"Yuhuan Zhang, Mengzhen Jia, Junyao Huang, Zhicheng Fu, Jiarong Liu, Yanlin Kang, Hui Yang, Bin Zhang","doi":"10.1021/acssynbio.5c00291","DOIUrl":"10.1021/acssynbio.5c00291","url":null,"abstract":"l-theanine is a nonproteinogenic amino acid widely utilized as a food additive and cosmetic ingredient, with promising potential in medical applications. Owing to its distinctive properties and well-established safety profile across the food, cosmetics, health supplement, and pharmaceutical sectors, global demand for l-theanine has been rising rapidly. Among available production methods, microbial fermentation, recognized for its environmental sustainability, has emerged as the preferred approach, gradually supplanting traditional techniques, such as plant extraction and chemical synthesis. Recent advances, particularly the development of ethylamine-free biosynthetic pathways and the successful de novo biosynthesis of l-theanine in Escherichia coli, a well-established microbial chassis with rapid growth and versatile genetic engineering tools, have opened new avenues for industrial-scale production. This review highlights the biosynthetic pathways of l-theanine and outlines targeted metabolic engineering strategies to enhance its yield. These include the identification and overexpression of key biosynthetic enzymes, enhancement of ATP regeneration, construction of endogenous ethylamine biosynthetic routes, redirection of metabolic flux toward l-theanine production, and suppression of competing pathways. Furthermore, current limitations in strain optimization are discussed along with perspectives on future directions for developing E. coli as a robust microbial cell factory for l-theanine production.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2433-2444"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Yi Yu, Jing Dai, Quan Yuan, Zihe Liu, Baisong Tong and Shuobo Shi*,

引用次数: 0

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Guillermo Nevot, Maria Pol Cros, Lorena Toloza, Nil Campamà-Sanz, Maria Artigues-Lleixà, Laura Aguilera and Marc Güell*,

引用次数: 0

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Xiaomeng Fu, Xiaoru Zuo, Kunqiang Hong, Chuanbo Zhang* and Wenyu Lu*,

引用次数: 0

Development of a Universal Platform for the Heterologous Expression of Bidirectional [Ni-Fe]-Hydrogenases in E. coli. 双向[Ni-Fe]-氢化酶在大肠杆菌中异源表达通用平台的建立

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18 Epub Date: 2025-06-16 DOI: 10.1021/acssynbio.5c00150

Dominik L Siebert, Frank Sargent, Ammar Al-Shameri, Volker Sieber

引用次数: 0

Xylose-Driven Metabolic Reprogramming in Saccharomyces cerevisiae for Enhancing p-Coumaric Acid Production. 木糖驱动的酿酒酵母代谢重编程提高对香豆酸产量。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18 Epub Date: 2025-06-27 DOI: 10.1021/acssynbio.4c00792

Yifei Zhao, Zhiqiang Xiao, Yongtong Wang, Xinjia Tan, Siqi Zhang, Qiyuan Lu, Fanglin Hu, Shasha Zuo, Yang Shan, Juan Liu, Gaoyang Li

{"title":"Xylose-Driven Metabolic Reprogramming in Saccharomyces cerevisiae for Enhancing p-Coumaric Acid Production.","authors":"Yifei Zhao, Zhiqiang Xiao, Yongtong Wang, Xinjia Tan, Siqi Zhang, Qiyuan Lu, Fanglin Hu, Shasha Zuo, Yang Shan, Juan Liu, Gaoyang Li","doi":"10.1021/acssynbio.4c00792","DOIUrl":"10.1021/acssynbio.4c00792","url":null,"abstract":"Xylose, the second most abundant sugar in nature, has garnered increasing attention as a promising carbon source for microbial fermentation in recent years. However, the unpredictable and inefficient metabolism of xylose in Saccharomyces cerevisiae has limited its practical application. In this study, we developed a xylotrophic strain through strategic integration of the xylose isomerase pathway, increasing xylose isomerase activity and identifying optimal transporters. Characterization of the modified strain demonstrated an 11.84-fold increase in ATP content under xylose conditions compared to glucose. This was achieved by redirecting carbon flux away from glycolysis, which resulted in a reduced level of ethanol and glycerol production. To demonstrate the industrial relevance of this platform, we applied the optimized strain to synthesize p-coumaric acid (p-CA). After process refinement, the strain achieved a final titer of 1293.15 mg/L p-CA using xylose as the sole carbon source, representing a 68.29% yield improvement compared to the glucose mode. To the best of our knowledge, this represents the highest reported to date for p-CA production from xylose alone. This study highlights the metabolic advantages of xylotrophic yeast and demonstrates the potential of leveraging these advantages for efficient p-CA synthesis, paving the way for the sustainable valorization of xylose into high-value natural products.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2514-2524"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Cyanobacterial Screening Platform for Rubisco Mutant Variants. Rubisco突变变体的蓝藻筛选平台。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18 Epub Date: 2025-07-07 DOI: 10.1021/acssynbio.5c00065

Ute A Hoffmann, Anna Z Schuppe, Axel Knave, Emil Sporre, Hjalmar Brismar, Elias Englund, Per-Olof Syrén, Elton P Hudson

{"title":"A Cyanobacterial Screening Platform for Rubisco Mutant Variants.","authors":"Ute A Hoffmann, Anna Z Schuppe, Axel Knave, Emil Sporre, Hjalmar Brismar, Elias Englund, Per-Olof Syrén, Elton P Hudson","doi":"10.1021/acssynbio.5c00065","DOIUrl":"10.1021/acssynbio.5c00065","url":null,"abstract":"Rubisco is the main entry point of inorganic carbon into the biosphere and a central player in the global carbon system. The relatively low specific activity and tendency to accept O2 as a substrate have made Rubisco an attractive but challenging target for enzyme engineering. We have developed an enzyme engineering and screening platform for Rubisco using the model cyanobacterium Synechocystis sp. PCC 6803. Starting with the Form II Rubisco from Gallionella, we first show that the enzyme can replace the native Form I Rubisco in Synechocystis and that growth rates become sensitive to CO2 and O2 levels. We address the challenge of designing a zero-shot input library of the Gallionella Rubisco, without prior experimental knowledge, by coupling the phylogenetically guided model EV mutation with \"in silico evolution\". This multisite mutagenesis library of Synechocystis (n = 16) was subjected to competitive growth in different gas feeds coupled to deep sequencing, in order to compare Rubisco variants. We identified an amino acid exchange that increased the thermostability of Gallionella Rubisco and conveyed resilience to otherwise detrimental amino acid exchanges. The platform is a first step toward high-throughput screening of Rubisco variants in Synechocystis and creating optimized enzyme variants to accelerate the Calvin-Benson-Bassham cycle in cyanobacteria and possibly chloroplasts.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2619-2633"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Hongwei Guo*, Yaqing Zeng, Mengqi Zhu, Tianqiu Huang, Weigao Wang, Catherine Madzak, Jun Zhao, Xiaohui Sun, Hongwen Chen* and Guo Chen,

引用次数: 0

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Yifei Zhao, Zhiqiang Xiao, Yongtong Wang, Xinjia Tan, Siqi Zhang, Qiyuan Lu, Fanglin Hu, Shasha Zuo, Yang Shan, Juan Liu* and Gaoyang Li*,

引用次数: 0