Journal of Medical Microbiology and Diagnosis最新文献_第8页

Haemophilus influenzae and Haemophilus parainfluenza in CysticFibrosis: 15 Years Experience 囊性纤维化中的流感嗜血杆菌和副流感嗜血杆菌:15年的经验

Journal of Medical Microbiology and Diagnosis Pub Date : 2015-08-28 DOI: 10.4172/2161-0703.S5-004

R. Ebbing, C. Robertson, P. Robinson

{"title":"Haemophilus influenzae and Haemophilus parainfluenza in CysticFibrosis: 15 Years Experience","authors":"R. Ebbing, C. Robertson, P. Robinson","doi":"10.4172/2161-0703.S5-004","DOIUrl":"https://doi.org/10.4172/2161-0703.S5-004","url":null,"abstract":"Background: Haemophilus influenzae and Haemophilus parainfluenzae are commonly identified in the lower airways of patients with cystic fibrosis (CF). Little is known of the change in prevalence and antimicrobial susceptibility in this population over time. We examined the epidemiology of both organisms over 15 years in our CF clinic. \u0000Results: 1538 isolates from respiratory specimens of 349 CF patients over 15 years were investigated. Annual prevalence increased significantly for both bacteria, being more pronounced for H. parainfluenzae. Average percentage of resistant cultures increased by 46% (H. Influenzae) and 61% (H. Parainfluenzae). For H. influenzae, resistance to ampicillin was 34.4%, co-trimoxazole 21.4%. For H. parainfluenzae, resistance to ampicillin was 50.0%, co-trimoxazole 26.8%. Resistance in H. influenzae and H. parainfluenzae to ampicillin and in H. parainfluenzae to amoxicillin/ clavulanic acid and co-trimoxazole increased over the study. \u0000Conclusion: This present study has shown an increased annual prevalence of H. influenzae and H. parainfluenzae in a large group of CF patients. Resistance to ampicillin significantly increased for H. influenzae and H. parainfluenzae, but increased resistance to amoxicillin/ clavulanic acid and co-trimoxazole was only significant in H. parainfluenzae.","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129126618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Acute Clostridium Difficile Infection in two Polish Pregnant Women with Premature Rupture of Membranes 急性难辨梭菌感染2例波兰孕妇胎膜早破

Journal of Medical Microbiology and Diagnosis Pub Date : 2015-08-21 DOI: 10.4172/2161-0703.S5-003

Joanna SkrÄt MagierÅo, Edyta BarnaÅ, R. Raa, Krzysztof Gutkowski, Leszek Juszczyk, A. Belkum, H. Pituch, Andrzej SkrÄt

引用次数: 0

Association of cag E Gene and LL-37 Serum Level with Helicobacterpylori-induced Peptic Ulceration in Egyptian Patients cag E基因和LL-37血清水平与埃及幽门螺杆菌致消化性溃疡的关系

Journal of Medical Microbiology and Diagnosis Pub Date : 2015-08-20 DOI: 10.4172/2161-0703.S5-002

A. Ibrahim, Rehab M. Atef, S. Mansour, M. Mansour, I. Ibrahim, T. Zaher

{"title":"Association of cag E Gene and LL-37 Serum Level with Helicobacterpylori-induced Peptic Ulceration in Egyptian Patients","authors":"A. Ibrahim, Rehab M. Atef, S. Mansour, M. Mansour, I. Ibrahim, T. Zaher","doi":"10.4172/2161-0703.S5-002","DOIUrl":"https://doi.org/10.4172/2161-0703.S5-002","url":null,"abstract":"Background and aim: Variation in the clinical outcome of Helicobacter pylori (H. pylori) induced pathology is multifactorial, involving a complex interplay between the host immune responses and pathogen virulence factors. Patients and methods: This study included 95 H. pylori infected patients who underwent endoscopy. They selected if culture and/or histopathological examination and rapid urease test were positive. All patients were examined for presence of cag E and LL-37. Results: Endoscopic findings in the patients were variable. The most frequent findings was gastritis 45.3% (43/95), followed by duodenitis; 36.8% (35/95), duodenal ulcer; 14.7% (14/95), esophagitis; 11.6% (11/95) and the least frequent one was gastric ulcer; 4.2% (4/95). Interestingly, cag E was positive in 27.4% of patients (26/95). As regards LL-37, its mean ± SD was 123.25 ± 20.26 ng/mL. Classifying studied patients into peptic ulcer and non peptic ulcer groups, cag E was positive in patients with peptic ulcer more than those who were non peptic (88.9 % versus 13%) (OR=0.019; CI 0.004-0.094) and P<0.001. The difference between two groups as regard LL-37 was statistically significant (CI 44.87-51.98), P<0.001. Conclusion: This study concluded that there was a strong association of cag E and LL-37 serum level with H. pylori-induced peptic ulceration in Egyptian patients.","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126767370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Middle East Respiratory Syndrome Coronavirus: Current Status and Future Implications 中东呼吸综合征冠状病毒:现状和未来影响

Journal of Medical Microbiology and Diagnosis Pub Date : 2015-08-08 DOI: 10.4172/2161-0703.1000200

Dereje Gedle, Mengistu Endris, B. Tessema, Setegn Eshetie, Temesgen Ewunetu, Fetuma Feyera, Gemechu Kumera

{"title":"Middle East Respiratory Syndrome Coronavirus: Current Status and Future Implications","authors":"Dereje Gedle, Mengistu Endris, B. Tessema, Setegn Eshetie, Temesgen Ewunetu, Fetuma Feyera, Gemechu Kumera","doi":"10.4172/2161-0703.1000200","DOIUrl":"https://doi.org/10.4172/2161-0703.1000200","url":null,"abstract":"In September 2012, a novel coronavirus was recognized, later renamed Middle East Respiratory Syndrome Coronavirus. This novel coronavirus belong lineage C of the genus Beta coronavirus included virus isolates from bats and camels. Fever, cough and shortness of breath were the common initial symptoms. On the other hand, majority of Patients were rapidly progressed to severe pneumonia and renal failure. Dromedary camels are suspected the primary reservoir for MERS CoV infection; suggesting camels to human transmission via contact with their excretion and consuming their product. However, human to human transmission occurred via the respiratory droplet or close contact. There is no specific drug or vaccine available for illnesses caused by MERS-CoV infection. Currently this novel virus is the major emerging respiratory pathogen threats of the world and capable of lethal human infections. Still new cases have been reported around the world particularly Arabian Peninsula. It has been also emerged outside Middle East countries which have not occurred before in South Korea and China since 20 May, 2015.","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130329381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Bacteraemia Caused by Kytococcus schroeteri in a Pneumonia Patient 1例肺炎患者由螺旋球球菌引起的菌血症

Journal of Medical Microbiology and Diagnosis Pub Date : 2015-08-08 DOI: 10.4172/2161-0703.1000199

Jihye Ha, K. Kim

引用次数: 2

Magnitude of Anemia and Associated Factors among Pregnant Women Attending Antenatal Care in Nekemte Health Center, Nekemte, Ethiopia 在埃塞俄比亚Nekemte卫生中心接受产前护理的孕妇贫血程度及相关因素

Journal of Medical Microbiology and Diagnosis Pub Date : 2015-08-05 DOI: 10.4172/2161-0703.1000197

Hylemariam Mihiretie, Motuma Fufa, Anane Mitiku, Chaltu Bacha, D. Getahun, Meselech Kejela, Getu Sileshi, Beletech Wakshuma

{"title":"Magnitude of Anemia and Associated Factors among Pregnant Women Attending Antenatal Care in Nekemte Health Center, Nekemte, Ethiopia","authors":"Hylemariam Mihiretie, Motuma Fufa, Anane Mitiku, Chaltu Bacha, D. Getahun, Meselech Kejela, Getu Sileshi, Beletech Wakshuma","doi":"10.4172/2161-0703.1000197","DOIUrl":"https://doi.org/10.4172/2161-0703.1000197","url":null,"abstract":"Background: Anemia is defined as a condition in which there is less than the normal hemoglobin (HB) level in the body, which decreases oxygen-carrying capacity of red blood cells to tissues. Anemia in pregnancy also leads to premature births, low birth weight, fetal impairment and infant deaths. The aim of this study was to determine the magnitude of anemia and associated factors among Pregnant Women Attending Antenatal Care in Nekemte Health Center, Nekemte, Ethiopia. \u0000 \u0000Methods: A cross-sectional study was conducted among Pregnant Women Attending Antenatal Care in Nekemte Health Center (NHC) between May 20, 2011 and June 25, 2011. A total of 150 pregnant women were selected continently. Blood specimen was collected and processed based on standard operating procedures where hemoglobin level was determined by Cell-Dyn1800. A structured questionnaire was used to collect data on Sociodemographic and associated risk factors. Data were entered and analyzed using SPSS version 20 software. Logistic regressions were applied to assess any association between explanatory factors and outcome variables. P values <0.05 were taken as statistically significant. \u0000Results: The total prevalence of anemia was 52%. Mild, moderate and severe anemia account for 50(64%), 17(21.8%) and 11(14.2%), respectively. Fifty three (68%) of the anemic pregnant women had normocytic normochromic RBCs. Anemia was significantly higher in pregnant women with diarrhea [AOR, 95% CI (5.6(1.7, 17.3), P<0.05] and in those with previous history of malaria [AOR, 95% CI (2.7(1.4, 9.33), P<0.05]. \u0000Conclusion: The prevalence of anemia in the study area is significantly high. Regular antenatal care follow up, adjustment of dietary and screening of parasitic infections are recommended to prevent impacts of anemia in","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126865676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

The Performance of Quantitative Real-Time Polymerase Chain Reaction and Galactomannan for Invasive Fungal Infections in Patients with Hematologic Malignancies 定量实时聚合酶链反应和半乳甘露聚糖检测恶性血液病侵袭性真菌感染的效果

Journal of Medical Microbiology and Diagnosis Pub Date : 2015-07-24 DOI: 10.4172/2161-0703.1000190

R. Reitzel

{"title":"The Performance of Quantitative Real-Time Polymerase Chain Reaction and Galactomannan for Invasive Fungal Infections in Patients with Hematologic Malignancies","authors":"R. Reitzel","doi":"10.4172/2161-0703.1000190","DOIUrl":"https://doi.org/10.4172/2161-0703.1000190","url":null,"abstract":"Background: Non-invasive methods to diagnose invasive fungal infections are needed to improve the poor outcome of these infections. We conducted this study to evaluate the diagnostic values of quantitative real time polymerase chain reaction and galactomannan in hematological malignancy patients. \u0000Results: Eighty-two consecutive patients with cancer were identified and prospectively followed for three \u0000months. Molecular testing using a quantitative polymerase chain reaction assay amplifying two primers 5.8S and 18S rRNA fungal genes and galactomannan was performed on 1540 blood samples and correlated with clinical data. Amplification of the 5.8S rRNA fungal gene had significantly higher sensitivity than amplification of the 18S rRNA gene for samples from either source (for blood samples: 90% vs 50%, p=0.007; for serum samples: 64% vs 5%, p<0.001). \u0000Conclusions: Galactomannan when used alone had a sensitivity of 38% and a specificity of 100%. The \u0000sensitivity of the combination assay of quantitative polymerase chain reaction plus galactomannan was significantly higher than that of GM test alone (71% vs 38%, p=0.03). Detection of the 5.8S rRNA fungal gene had significantly higher sensitivity than the 18S rRNA gene for samples from either source blood or serum. The combination of quantitative polymerase chain reaction plus galactomannan assay improved the diagnostic value of invasive fungal infections.","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123363782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Silencing with Herbal Compounds against Bacteria 用草药化合物抑制细菌的基因沉默

Journal of Medical Microbiology and Diagnosis Pub Date : 2015-07-09 DOI: 10.4172/2161-0703.1000196

Moradi Fateme, Esmaeili Davoud, Hossein Saghi, Parya Baban Zadeh

引用次数: 2

Acute Blindness in a Child after Quinine treatment for Severe Malaria 重症疟疾奎宁治疗后儿童急性失明

Journal of Medical Microbiology and Diagnosis Pub Date : 2015-06-22 DOI: 10.4172/2161-0703.S3-007

V. Orish, Ilechie A. Alex

引用次数: 0

Sperm-Impairing Escherichia coli, Alcohol and Male Infertility: An In Vivo Study 损害精子的大肠杆菌、酒精和男性不育症:体内研究

Journal of Medical Microbiology and Diagnosis Pub Date : 2015-06-09 DOI: 10.4172/2161-0703.1000195

P. Bhardwaj, Kalpana Rana, Harpreet, Er, Praveen Bh, Ari, V. Prabha

{"title":"Sperm-Impairing Escherichia coli, Alcohol and Male Infertility: An In Vivo Study","authors":"P. Bhardwaj, Kalpana Rana, Harpreet, Er, Praveen Bh, Ari, V. Prabha","doi":"10.4172/2161-0703.1000195","DOIUrl":"https://doi.org/10.4172/2161-0703.1000195","url":null,"abstract":"Purpose: Establishment of a male Balb/c mouse model to study the role of sperm immobilizing E. coli with or without alcohol on mouse reproductive potential. \u0000Materials and Methods: The mice in the experimental groups were intraperitoneally administered with different doses (104, 106 or 108 cfu) of sperm immobilizing E. coli alone or in combination with alcohol and as control group alcohol alone or PBS for 10 consecutive days. The groups were evaluated for change in body weight, seminal parameters, bacterial load and histopathological changes. \u0000Results: Weight gain was observed only in mice receiving PBS, whereas there was decrease in weight in all the groups except the group receiving 104 cfu where weight remained constant. No significant change in tissue somatic index was observed in any group. Reduced motility and viability was noticed in all groups except group receiving PBS. Decapitation was observed in groups instilled with 104 cfu and 104 cfu along with alcohol. Seminal parameters could not be evaluated due to non-specific aggregation of sperms in group administered with 106 and 108 cfu alone as well as with alcohol. Histopathological studies of testes showed very few mature spermatozoa and maturation arrest at later stage in all groups except PBS group. \u0000Conclusion: Intraperitoneal inoculation with sperm immobilizing E. coli with or without alcohol consumption could lead to alteration of semen parameters, induction of decapitation in spermatozoa, thereby decreasing the reproductive potential of male mice.","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116896854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3