World Congress of the International Photodynamic Association最新文献_第2页

Development of anti-cadherin-17 antibody -IR700 conjugate for photodynamic therapy against gastrointestinal cancers 抗钙粘蛋白-17抗体-IR700偶联物光动力治疗胃肠道肿瘤的研究

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525313

Y. Lum, D. Ng, J. Luk, W. Fong

引用次数: 1

Superiority of diagnosis and treatment with multidisciplinary team in vascular anomalies 多学科团队在血管异常诊治中的优势

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2523879

Xian Jiang, Lian Liu

{"title":"Superiority of diagnosis and treatment with multidisciplinary team in vascular anomalies","authors":"Xian Jiang, Lian Liu","doi":"10.1117/12.2523879","DOIUrl":"https://doi.org/10.1117/12.2523879","url":null,"abstract":"Vascular anomalies are mainly divided into two types: vascular tumors and vascular malformations. According to biologic characteristics, vascular tumors are classified into benign, locally invasive and malignant tumor. Vascular malformations are composed of capillary malformations, venous malformations, arterial malformations and so on. The clinical manifestations of vascular anomalies, involving skin, mucosa membrane, bones and muscles of extremities and eyes, are extremely complicated, therefore multidisciplinary team (MDT) of vascular anomalies, including department of dermatology, plastic surgery, invasive technology, ophthalmology, radiology, ultrasound, pathology, should be included. Dermatologists are good at diagnosing vascular anomalies by differentiating the lesions with eyes or dermoscopy, but we often require the assistance from ultrasonography for determining flow, velocity, direction of blood. The diagnosis level of ultrasound doctors is closely associated with clinical experience, hence we also need the aid from radiologists who can use vascular sequence in MRI or CT to detect vessels. Histopathology, confirming benign or malignant tumors, tissue origin, can decide the treatment scheme and operation mode. Laser, intense pulsed light and photodynamic therapy are insufficient for treating complicated vascular anomalies in dermatological research, and excision, skin-grafting and interventional therapy are applied to treat these diseases. Patients with vascular diseases generally visit different departments which are sometimes improper. In order to diagnose and treat vascular anomalies better, relevant disciplines should strengthen communication. Our MDT of vascular anomalies already consulted 20 patients and the patients acquired the right and effective treatment. Therefore, it is necessary to establish MDT of vascular diseases which can improve the rate of correct diagnosis and give a right treatment.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132559977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of heme biosynthesis on the mitochondrial functions in C2C12 cels 血红素生物合成对C2C12细胞线粒体功能的影响

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525559

A. Suprihadi, H. Lai, H. Tan, Kiwamu Takahashi, M. Nakajima, Tohru Tanaka, S. Ogura

引用次数: 0

Stimulation of apoptosis in systematically crossed cancer cell cultures by exudates from real tumors after PDT PDT后真实肿瘤渗出物对系统交叉癌细胞培养细胞凋亡的刺激

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525227

V. Purtskhvanidze, Yu. P. Simakov, Dzhivan Rostomyan

引用次数: 0

Targets of photodyamic inactivation in fungal cells 真菌细胞光动力失活的靶点

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2537128

A. Baptista, S. Núñez, A. A. Martin, M. Ribeiro

{"title":"Targets of photodyamic inactivation in fungal cells","authors":"A. Baptista, S. Núñez, A. A. Martin, M. Ribeiro","doi":"10.1117/12.2537128","DOIUrl":"https://doi.org/10.1117/12.2537128","url":null,"abstract":"Photodynamic inactivation (PDI) has been reported to be effective to eradicate a wide variety of pathogens, including antimicrobial-resistant microorganisms. However, there are conflicting reports in the literature about the effect of growth phase on the susceptibility to PDI. The aim of this study was to identify the potential molecular targets of PDI on Candida albicans in exponential growth phase after PDI mediated by methylene blue (50μM) and exposure to a 660nm-LED (P=360mW). For this task, scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) techniques were employed. Pre-irradiation time was set at 10min and exposure time was 15 min delivering a radiant exposure of 162 J/cm2 on a 24-well plate of about 2 cm2. Morphological analysis revealed cell damage after PDI. FT-IR predominantly showed degradation of functional groups related to C-O of deoxyribose; C-C of DNA; C-O stretching vibration of C-OH group of ribose-RNA; P-O stretching modes from the phosphodiester groups of nucleic acids; C=C, C=N, C=O, N=H proteins and amides. Previous studies from our group had demonstrated different targets on the same cells but in stationary growth phase. Therefore, we can conclude that PDI promoted damage to intracellular structures in fungal cells at exponential-phase growth and information on the susceptibility of different growth phases to PDI can be of great importance for the development of treatment strategies that would lead to inactivation of fungal cells in all possible phases of growth in a way that would turn the clinical PDI treatment effective and predictable.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124866981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of in-vivo tissue optical properties for anal photodynamic therapy 肛门光动力疗法体内组织光学特性的测定

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525624

Andrew C. Li, Y. Ong, A. Dimofte, T. Busch, T. Zhu

引用次数: 3

Determining an in vitro dose-response relationship of photodynamic therapy with first and second-generation photosensitisers for high grade tumours 确定第一代和第二代光敏剂光动力治疗高级别肿瘤的体外剂量-反应关系

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2527574

Kirit Singh, D. Baptista‐Hon, Molly Hewitt, O. Kouli, K. Hossain-Ibrahim, T. Hales

{"title":"Determining an in vitro dose-response relationship of photodynamic therapy with first and second-generation photosensitisers for high grade tumours","authors":"Kirit Singh, D. Baptista‐Hon, Molly Hewitt, O. Kouli, K. Hossain-Ibrahim, T. Hales","doi":"10.1117/12.2527574","DOIUrl":"https://doi.org/10.1117/12.2527574","url":null,"abstract":"Photodynamic Therapy (PDT) is used with photosensitizing agents to enhance the extent of tumour destruction intra-operatively, thereby enhancing survival. However, high-grade tumour types demonstrate significant heterogeneity and their susceptibility to PDT may vary. This study aimed to determine the response of different invasive tumour types to different photosensitizers and differing light irradiation doses. Immortalized neuroblastoma (SHSY-5Y), proliferative human keratinocytes (HaCaT) and Glioblastoma Multiforme (U87MG) cell lines were exposed in vitro to progressively increasing 630nm laser irradiation, with the use of a first (5- Aminolevulinic Acid) and second-generation photosensitizer (Photofrin). Cell kill was assessed using a viability assay, quantified by a 96-well plate reader. Individually, neither irradiation, 5-aminolevulinic acid (ALA) or Photofrin caused death of U87MG, SHSY-5Y or HaCaT cells. However, when combining light irradiation with photosensitizers, both 5-ALA and Photofrin caused a dose-dependent reduction in the viability of U87MG glioblastoma cells, but the potency of light was higher in cells treated with Photofrin. SHSY-5Y neuroblastoma cells exhibited higher sensitivity to PDT (using 5-ALA) than U87MG and HaCat keratinocytes. SHSY-5Y and U87MG exhibited similar sensitivities to irradiation with Photofrin as the photosensitizer. Highly invasive tumours can demonstrate different behaviours when exposed to similar PDT doses with certain photosensitizers, while others produce a uniformity of response. This has strong implications for ongoing research, suggesting a mechanism beyond simple free radical generation, as well as the potential for those tumours exhibiting a poor response to PDT in prior work requiring investigation with other photosensitizers.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116725016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety and efficacy of photosensitizer Photolon (Fotolon) in photodynamic therapy 光敏剂Photolon (Fotolon)在光动力治疗中的安全性和有效性

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2528083

T. Trukhachova

{"title":"Safety and efficacy of photosensitizer Photolon (Fotolon) in photodynamic therapy","authors":"T. Trukhachova","doi":"10.1117/12.2528083","DOIUrl":"https://doi.org/10.1117/12.2528083","url":null,"abstract":"Photosensitizer Photolon, a complex of chlorin e6 and PVP, was originally developed by RUE “Belmedprepa, and registered in Belarus, Russian Federation, Kazakhstan and Ukraine. During the last 18 years Photolon was used clinically and proven to be effective in photodynamic diagnostics and treatment of different cancerous and benign conditions. Pre–marketing clinical trials of Photolon were conducted at 4 major oncology centers in Minsk, Obninsk, and Moscow and total of 112 patients were included. Open-label randomized non-comparative clinical trial confirmed safety and efficiency of PDT with Photolon for 154 patients with CIN grade I-III. Clinical trial of PDT with Photolon was carried out in 68 patients with disseminated skin melanoma. Clinical trial was conducted in 37 patients with central lung carcinoma. In 2006 we started an open-label randomized controlled clinical trial of Photolon applied intraoperatively in patients with primary and metastatic brain tumors, 102 patients were treated. In 2006 an open-label multicenter clinical trial of Photolon for treatment of choroidal neovascularization (CN) in age-related macular degeneration and myopic maculopathy was complete. 50 patients with CN were treated and positive therapeutic effect was maintained in 100% of patients within 1 year. Since 2001 more then 35 000 doses of Photolon were produced, and about 20 000 patients were treated. During this time there were no reports of adverse events or failed treatment with Photolon. Photolon was proven to be an extremely effective photosensitizer for PTD for multiple medical conditions and has minimal side effects.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113971532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Thomas J. Dougherty: present at the creation 托马斯·j·多尔蒂:在创作现场

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525475

D. Kessel

引用次数: 0

Comparative analysis of 5-ALA-induced fluorescence intensity and 11C-Methionine PET uptake in glioma surgery 胶质瘤手术中5- ala诱导的荧光强度与11c -蛋氨酸PET摄取的比较分析

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2524171

Kazuhide Shimizu, M. Inaji, Yoji Tanaka, K. Tamura, T. Sugawara, K. Ishii, T. Nariai, T. Maehara

{"title":"Comparative analysis of 5-ALA-induced fluorescence intensity and 11C-Methionine PET uptake in glioma surgery","authors":"Kazuhide Shimizu, M. Inaji, Yoji Tanaka, K. Tamura, T. Sugawara, K. Ishii, T. Nariai, T. Maehara","doi":"10.1117/12.2524171","DOIUrl":"https://doi.org/10.1117/12.2524171","url":null,"abstract":"Background: 5-aminolevulinic acid (5-ALA) induces red fluorescence in malignant brain tumors that has been used for intraoperative guidance for tumor removal. Positron emission tomography with 11C-Methionine (Met-PET) is a promising imaging modality to depict clear boundary of infiltrating glioma. We studied the correlation between preoperative Met-PET uptake and intraoperative 5-ALA fluorescence. Method: Patients who underwent preoperative Met-PET study and tumor removal using 5-ALA over a period of two years were analyzed. The regional uptake of Met-PET was expressed as the ratio of the maximum of standardized uptake value (SUV-max) to the contralateral normal brain. 5-ALA fluorescence from tumor sample was immediately measured during surgery using blue laser and spectrometer. Fluorescence intensity was categorized into four groups (None, Weak, Moderate, Strong) with ten-hold differences. Results: 16 cases with pathological diagnosis of astrocytic tumors were analyzed. Met-PET uptake was markedly high in “Strong” fluorescence group (3.81±0.77). Particularly, among 11 newly-diagnosed cases, the difference was statistically significant (p=0.017, Kruskal-Wallis test). \"Strong\" fluorescence group still showed significant difference when compared with other groups together, in all cases and newly-diagnosed cases (p=0.01 and p=0.004, respectively, Mann- Whitney U test). Discussion/Conclusion: There are limited number of reports regarding 5-ALA and Met-PET, stating that Met-PET and 5- ALA fluorescence should be considered separately. However, the fluorescence was grossly distinguished whether positive or negative. Our study utilized objective spectroscopic measurement of fluorescence intensity. The results suggested that strong fluorescence intensity induced by 5-ALA reflects high Met-PET uptake. This could indicate the importance of bright fluorescence by 5-ALA in glioma surgery.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121160976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0