{"title":"Molecular detection of Babesia microti in one-humped camel (Camelus dromedarius) in Halayeb and Shalateen, Halayeb, Egypt","authors":"M. Rizk","doi":"10.21608/evmspj.2021.204355","DOIUrl":"https://doi.org/10.21608/evmspj.2021.204355","url":null,"abstract":"Mohamed Abdo Rizk 1, * Mohamed A. ElAdl , Mostafa Al-Araby , Mayar O. Ali , Shimaa Abd El-Salam El-Sayed , Mohamed El-Beskawy , Naglaa A. Gomaa Addresses 1 Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt 2 National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, Japan 3 Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt 4 Department of Parasitology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt 5 Department of Animal Genetics, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt 6 Department of Internal Medicine (infectious diseases), Faculty of Veterinary Medicine, Matrouh University, Egypt. 7 Department of Animal Medicine, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt. *Corresponding author Mohamed Abdo Rizk, PhD Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. Tel.: +201019474865; Fax: + 20502379952; E-mail address: dr_moh_abdo2008@mans.edu.eg Accepted: January 2021 Abstract","PeriodicalId":266046,"journal":{"name":"Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131200206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Efficacy of triage parasite panel in diagnosis of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum antigens in symptomatic children stool specimens","authors":"Gamal AboSheishaa","doi":"10.21608/evmspj.2021.192801","DOIUrl":"https://doi.org/10.21608/evmspj.2021.192801","url":null,"abstract":"","PeriodicalId":266046,"journal":{"name":"Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)","volume":"318 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134143159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Efficacy of allicin oil and diclazuril on broiler chickens: Effect on caecal coccidiosis and caecal tissue antioxidant","authors":"Amany Abd-Elghany","doi":"10.21608/evmspj.2021.297930","DOIUrl":"https://doi.org/10.21608/evmspj.2021.297930","url":null,"abstract":"","PeriodicalId":266046,"journal":{"name":"Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126429988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First time recording of seasonal prevalence of Cysticercus tenuuicollis among small ruminants at Matrouh City,Egypt.","authors":"Nadia Laban","doi":"10.21608/EVMSPJ.2020.142941","DOIUrl":"https://doi.org/10.21608/EVMSPJ.2020.142941","url":null,"abstract":"","PeriodicalId":266046,"journal":{"name":"Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122066984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prevalence and Phylogenetic analysis of Fasciola species in Upper Egypt Based on Ribosomal ITS-2 gene Sequencing","authors":"Reda Khalafala","doi":"10.21608/evmspj.2020.132162","DOIUrl":"https://doi.org/10.21608/evmspj.2020.132162","url":null,"abstract":"142 Ahmed G. Abdelazeem, Amer Ragheb Abdelaziz, Reda E. Khalafalla, Mostafa F.N. Abushahba, 1 Department of Parasitology, Faculty of Veterinary Medicine, Aswan University, Egypt. Department of Parasitology, Faculty of Veterinary Medicine, Sohag University, 82524, Egypt. Department of Parasitology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, 33516, Egypt. Department of Zoonoses, Faculty of Veterinary Medicine, Assiut University, 71526, Egypt. Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO 63110, USA.","PeriodicalId":266046,"journal":{"name":"Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128964547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Improved DNA extraction technique from blood clot for diagnosis of Trypanosoma evansi in camels","authors":"Amira W. Deweir","doi":"10.21608/evmspj.2020.71062","DOIUrl":"https://doi.org/10.21608/evmspj.2020.71062","url":null,"abstract":"The detection of Trypanosoma evansi genetic material in clinical samples is considered an important diagnostic tool. When blood clots can be a valuable source of genetic information. In our paper, Fourty blood samples were collected from camels at Cairo abbatoir in plane tubes. Blood clots were dispersed by scissor and treated by lysis buffer and proteinase-K, then DNA was extracted according to protocoal of Gene JET Whole Blood Genomic DNA Purification Mini Kit. the quality and integrity of the extracted DNA were corroborated by agarose gel electrophoresis and conventional PCR. Results confirmed that DNA purified from clotted blood can be used in further amplifications. Moreover 16 out of 40 samples (40%) were positive in PCR with bands at the expected molecular weight (164pb). The objective of the present study was to improve and develop a more sensitive method to recover T. evansi DNA from clot samples.","PeriodicalId":266046,"journal":{"name":"Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131208782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Parasitological Prevalence of Cryptosporidium infection in calves, chickens and human in Behera province","authors":"Mohamed Bessat","doi":"10.21608/evmspj.2019.69608","DOIUrl":"https://doi.org/10.21608/evmspj.2019.69608","url":null,"abstract":"Cryptosporidium is a cosmopolitan intestinal protozoan parasite that belongs to the phylum Apicomplexa and infects a broad range of vertebrate hosts including livestock, human and birds. This current study was aimed to investigate the prevalence of Cryptosporidium spp.infection in young calves, human and chickens, in five districts covering five different geographical locations of the Behera province. In doing this, a total number of 310 faecal samples from calves (n= 148), human being (n=112) and chicken (n=50) were collected, stained using the modified Ziehl-Neelsen staining method, and were examined microscopically for Cryptosporidium spp. oocysts. Correlations between prevalence rates and related parameters such as species, age, gender and faecal consistencies were also analyzed. Results indicated that 43.2%, 16.1% and 6%, of calves, human and chicken, were found infected with Cryptosporidium spp., respectively. Statistically significant variable rates of infection were also detected across six surveyed districts of the Behera governorate, with the notable absence of infection from chicken in Abo Hummus and Rahmaneyah districts. Statistically significant variable rates of infection were also characterized in calves when species, age, sex, and faecal consistency were analyzed; buffalo >4 weeks calves, male and calves with formed faeces have higher infection rates than their counterparts of cattle Cryptosporidium spp. in the livestock and chicken living in close proximity to human population necessitates better surveillance and control measures to protect vulnerable animal and human populations.","PeriodicalId":266046,"journal":{"name":"Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125043600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First phylogenetic evidence of Trichuris spp. affecting Hamadryas baboon (Papio Hamadryas) in Egypt.","authors":"Amira W. Deweir","doi":"10.21608/EVMSPJ.2019.69620","DOIUrl":"https://doi.org/10.21608/EVMSPJ.2019.69620","url":null,"abstract":"Trichuris spp., known as whipworms, are recognized to infect numerous mammalian species including humans and non-human primates. In the present study, forty five fecal samples collected from Hamadryasis baboon at Alexandria Zoo, Egypt were examined microscopically using concentration sedimentation and flotation techniques to detect prevalence of Trichuris spp. Furthermore,the genetic variation of Trichuris spp. recovered from naturally infected Hamadryasis baboon , was analyzed using the ribosomal DNA (ITS) as molecular marker by PCR and sequencing. By combining this dataset with Genbank records for Trichuris isolated from other humans, non-human primates and pigs and phylogenetic analysis, we proved the presence of two distinct Trichuris genotypes that infect baboons, T. trichura and T. suis., which is important for the ongoing treatment of Trichuris that estimated to infect 600 million people worldwide. To our knowledge this is the first study in Egypt genetically characterized Trichuris spp in Hamadryasis baboon, with future prospective to detect genetic characterization of Trichuris in other non-human primates.","PeriodicalId":266046,"journal":{"name":"Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122813846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pharmacokinetics and Bioavailability of cefotaxime in broiler chickens","authors":"Mohamed Elhewaty","doi":"10.21608/evmspj.2019.99307","DOIUrl":"https://doi.org/10.21608/evmspj.2019.99307","url":null,"abstract":"E-mail: melhewaty@yahoo.com Abstract: After a single intravenous (IV) and intramuscular (IM) injection in broiler chickens, the disposition kinetic profile of cefotaxime was explored. Cefotaxime was administered at 10 mg / kg b.wt dose level for both routes. The serum cefotaxime concentrations estimated at 0.08 h were 47.06 μg / ml after IV injection, which gradually decreased and cefotaxime was identified up to 12 h (0.88 μg / ml). In broiler chickens, the average values of Cltot, Vdss and T0.5 β of cefotaxime were 0.09 L kg -1 h -1 , 0.38 L kg -1 and 2.49 h. The highest serum concentration (Cmax) after IM injection was (22.21 ± 2.03 μg / ml), the maximum serum concentration period (tmax) was (1.16 ± 0.11h) and the half-life of elimination (T0.5 el) was (3.24 ± 0.31h). Bioavailability after IM injection was 84.27%, and in vitro protein binding percent was 28.79 %. A recommended IM dosage for cefotaxime in broiler chickens would be 10 mg / kg b.wt., administered intramuscularly at 12 h intervals, providing a therapeutic serum concentration in broiler chickens exceeding the MIC ≤ 0.5 μg/ml for most sensitive bacterial pathogens in broiler chickens.","PeriodicalId":266046,"journal":{"name":"Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127507764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First finding of antibodies to Encephalitozoon cuniculi in raised chickens in Egypt","authors":"pKaram Imam Ashmawy, Somaia Seif Abuakkadap","doi":"10.21608/EVMSPJ.2019.80817","DOIUrl":"https://doi.org/10.21608/EVMSPJ.2019.80817","url":null,"abstract":"20 Abu-Akkada s.s* ;Ashmawy K and EL shanat s. Department of Parasitology, Faculty of Veterinary Medicine, Alexandria University Abis, Alexandria, Egypt Post Code: 21944 *Author of correspondence: Somaia Saif Abu-Akkada Professor of Parasitology Head of Department of Parasitology Acting Vice dean of Postgraduates and researches affairs. Faculty of Veterinary Medicine Alexandria University Egypt E.mail: somaia_abuakkada@yahoo.com Tel. +2 045 2960306 Fax: +2 045 2960450 Abstract:","PeriodicalId":266046,"journal":{"name":"Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130535338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}