Improved DNA extraction technique from blood clot for diagnosis of Trypanosoma evansi in camels

Abstract

The detection of Trypanosoma evansi genetic material in clinical samples is considered an important diagnostic tool. When blood clots can be a valuable source of genetic information. In our paper, Fourty blood samples were collected from camels at Cairo abbatoir in plane tubes. Blood clots were dispersed by scissor and treated by lysis buffer and proteinase-K, then DNA was extracted according to protocoal of Gene JET Whole Blood Genomic DNA Purification Mini Kit. the quality and integrity of the extracted DNA were corroborated by agarose gel electrophoresis and conventional PCR. Results confirmed that DNA purified from clotted blood can be used in further amplifications. Moreover 16 out of 40 samples (40%) were positive in PCR with bands at the expected molecular weight (164pb). The objective of the present study was to improve and develop a more sensitive method to recover T. evansi DNA from clot samples.