Ahmed Ibrahim Younus, M. Yousef, Maher Abdel-NabiKamel, Rakhad Alrawi, J. Abdulrahman
{"title":"Toxicity of iron oxide nanoparticles, silver nanoparticles and their mixture on antioxidant enzymes and free radicals in male rats","authors":"Ahmed Ibrahim Younus, M. Yousef, Maher Abdel-NabiKamel, Rakhad Alrawi, J. Abdulrahman","doi":"10.30574/MSABP.2021.1.2.0001","DOIUrl":"https://doi.org/10.30574/MSABP.2021.1.2.0001","url":null,"abstract":"In the present study Wistar male rats were used. Rats were divided into 4 equal groups, 10 rats each. Group 1 served as control, group 2 was administered orally with Fe2O3NPs (5 mg/kg BW; >50 nm), group 3 was treated intraperitoneally with AgNPs (50 mg/kg BW; >100 nm) and group 4 was administered with the mixture of Fe2O3NPs with AgNPs. Animals were treated with the doses every day for 79 days. Results showed significant (P < 0.05) decrease in the antioxidant enzymes (GPX, GST, CAT and SOD) and reduced glutathione (GSH) and total antioxidant capacity (TAC), while significant (P < 0.05) increase in thiobarbituric acid-reactive substances (TBARS) and nitric oxide (NO) in plasma and testes of rats treated with Fe2O3NPs, AgNPs and their combination compared to control group.","PeriodicalId":263752,"journal":{"name":"Magna Scientia Advanced Biology and Pharmacy","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131533764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Konstantin Andreevich Kuznetsov, Pavel Semenovich Kizim, Andrey Yurievich Berezhnoy, Oleksandr, Pilipovich Shchus, Gennadiy Michailovich Onyshchenko
{"title":"Cell stress response to low-dose neutron radiation","authors":"Konstantin Andreevich Kuznetsov, Pavel Semenovich Kizim, Andrey Yurievich Berezhnoy, Oleksandr, Pilipovich Shchus, Gennadiy Michailovich Onyshchenko","doi":"10.30574/msabp.2020.1.1.0022","DOIUrl":"https://doi.org/10.30574/msabp.2020.1.1.0022","url":null,"abstract":"Background. It is a point of discussion whether low-dose ionizing radiation has harmful or stimulating impact on cell. According to high relative biological effectiveness of neutron radiation there is a need of description of any process triggered in the cell by neutrons. Objective. The aim of current work is the investigation of the low dosed neutron radiation effects on human cells by indicators of cell stress such as state of chromatin and cell membrane permeability. Materials and methods. Human buccal epithelium cells from 3 male donors (21, 24, 25 years old) were exposed to fast neutron radiation in dose range 2.3–146.0 mSv from 239Pu-Be source. State of chromatin was evaluated by count of heterochromatin granules quantity in 100 nuclei stained with 2% orcein in 45% acetic acid; ratio of cells with increased membrane permeability stained with 5 mM indigocarmine in 300 cells. Results. Changes in level of heterochromatin granules quantity and in cell membrane permeability revealed wave-shaped dependency with maximum effects at 36.5 mSv. Further increase of dose resulted in return of both chromatin state and membrane permeability levels closely to control or even lower. Conclusion. Membrane restoration and chromatin decompaction under doses higher than 36.5 mSv together can be a sign of hormetic (stimulating) effect of low-dose neutron radiation.","PeriodicalId":263752,"journal":{"name":"Magna Scientia Advanced Biology and Pharmacy","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117209116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}