Seyede Yalda Raeesi Sadati, Sodabeh Jahanbakhsh Godehkahriz, A. Ebadi, M. Sedghi
{"title":"Study of Expression Pattern of Some Transcription Factors in Wheat under Drought Stress and Zinc Nanoparticles","authors":"Seyede Yalda Raeesi Sadati, Sodabeh Jahanbakhsh Godehkahriz, A. Ebadi, M. Sedghi","doi":"10.52547/pgr.7.2.11","DOIUrl":"https://doi.org/10.52547/pgr.7.2.11","url":null,"abstract":"Under drought stress condition, the signaling system induces expression of certain genes to counteract the deleterious effects of environmental stress. Among the essential micronutrients for plant growth and development, zinc has an important role in many plant metabolic processes including gene expression and stress tolerance. In order to investigate the effect of drought stress and ZnO on relative expression pattern of some genes involved in abiotic stresses (including WRKY1, HMA2 and ZIP1 genes) in wheat cultivars, a factorial experimental was conducted in pot condition based on a completely randomized design with three replications. In this experiment, the first factor was three levels of drought stress (35, 60 and 85% of field capacity), the second factor was three wheat cultivars (including Heidari, Meihan and Sysons), and the third factor was three levels of ZnO (0, 0.5 and 1 g/l). According to the results, with increasing the level of drought stress, the relative expression of WRKY1 and ZIP1 genes in drought tolerant cultivar (Meihan), and also with increasing nanoparticle concentration over stress time, the expression of ZIP1 gene in drought sensitive cultivar (Sysons) increased. The highest relative expression of HMA2 gene was observed in Heidari cultivar under mild drought stress. Generally, the expression of all three genes studied in tolerant cultivar (Meihan) increased under drought stress. Increasing the expression level of HMA2 and ZIP1 genes could be related to the transfer of zinc to consuming tissues and also, to increase the consumption of zinc in current metabolism of plant, which is important in tolerance of wheat to drought stress.","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132600361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sahar Dashchi, H. Rahnama, K. Cheghamirza, Katayun Zamani
{"title":"Construction of Plant Expression Vectors Harboring WRI1 and LPAAT Genes and Its Transformation in Tobacco Plants","authors":"Sahar Dashchi, H. Rahnama, K. Cheghamirza, Katayun Zamani","doi":"10.52547/pgr.7.2.4","DOIUrl":"https://doi.org/10.52547/pgr.7.2.4","url":null,"abstract":"","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"421 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126712223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of SSR Markers for Genetic Segregation of Some Commercial Date Palm Cultivars","authors":"M. Rezaei, Abdoreza Kavand","doi":"10.52547/pgr.7.2.8","DOIUrl":"https://doi.org/10.52547/pgr.7.2.8","url":null,"abstract":"","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125403057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Razieh Azizian Mosleh, M. Abdollahi, H. Sarikhani, A. Mirzaie-asl, Payam Pour Mohammadi
{"title":"Study of the Effect of 5-Azacytidine as a DNA Demethylating Agent on Agronomic Traits, Androgenesis Induction via Anther Culture and DNA-Methyltransferase Gene Expression in Maize (Zea mays L.) Leaf Tissue","authors":"Razieh Azizian Mosleh, M. Abdollahi, H. Sarikhani, A. Mirzaie-asl, Payam Pour Mohammadi","doi":"10.52547/pgr.7.2.10","DOIUrl":"https://doi.org/10.52547/pgr.7.2.10","url":null,"abstract":"Optimization of in vitro methods for the production of maize double haploids plays an important role in the breeding programs of this plant. In this study, the effects of 5-azacytidine on agronomic traits, androgenesis induction efficiency and also, DNA methyltransferase gene expression (AF229183.1) in two growth stages of maize were investigated. This experiment was performed as factorial based on a completely randomized block design with three replications. Two maize genotypes (DH5 × DH7 and ETMH-82) were considered as the first factor and treatment of maize seeds with 5-azacytidine (0, 5, 10, and 100 μM) was considered as the second factor. The maize seeds were sowed in the field and during the growth stages, various morphological and agronomic traits were recorded. In the anther culture experiment, the suitable anthers containing microspores at mid to late-uninucleate stages were selected and cultured in an YPm culture medium containing 1 mg/l 2, 4-D, and 2 mg/l BAP. Interaction effects of genotype and 5-azacytidine concentrations showed significant differences for the majority of studied traits except for number of kernel per ear row, kernel depth, plant diameter, number of leaves and number of ears. The highest amounts of 1000-kernel weight were obtained with treatments of 10 and 100 μM and the highest ones for grain yield and biological yield traits were obtained with 100 μM 5azacytidine treatment for both genotypes. Seeds of DH5 × DH7 genotype treated with 5 μM 5azacYtidine produced the highest mean number of embryo-like structures (0.1833) and regenerated plantlets (0.067) per each anther. Relative expression of DNA methyltransferase gene in maize seeds treated with different concentrations of 5-azacytidine showed a significant decrease in both genotypes and both growth stages compared to control plants (treated with 0 μM 5-azacytidine), that this decrease in gene expression could lead to improved androgenesis induction in anther culture of DH5 × DH7 genotype. However, despite the decrease in expression of this gene in two growth stages of ETMH-82 genotype, androgenesis induction was not observed in this genotype. The results of the present study can help to determine the role of epigenetic factors in androgenesis induction and improving the production of haploid plants in maize.","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125764073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation the Mean Performance and Stability of Rice Genotypes by Combining Features of AMMI and BLUP Techniques and Selection Based on Multiple Traits","authors":"P. Sharifi, A. Abbasian, A. Mohaddesi","doi":"10.52547/pgr.7.2.13","DOIUrl":"https://doi.org/10.52547/pgr.7.2.13","url":null,"abstract":"","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134252559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of QTLs Controlling Agronomical Traits in Rice Population Derived from Cross of Tarom Landrace and Tarom Mutant","authors":"M. Katouzi, S. Navabpour, H. Sabouri, A. Ebadi","doi":"10.29252/PGR.7.2.5","DOIUrl":"https://doi.org/10.29252/PGR.7.2.5","url":null,"abstract":"In order to identify QTLs controlling agronomically traits, landrace Tarom and rice Tarom mutant were crossed. SSR, ISSR, iPBS and IRAP markers were amplified in 250 F2 individuals to prepare the linkage map. Number of tillers, 100 grain weight, number of filled grains, number of unfilled grains, plant height, panicle length, number of branches, stem diameter, grain length, grain width, grain shape, straw weight, days to maturity, flag leaf length and flag leaf width were measured for 250 individuals. The linkage map covered 970.9 cM of rice genome. The distance between two adjacent markers was calculated to be 12.77 cM. Based on the results, a total of 13 QTLs were identified for the evaluated traits. For all studied traits, alleles transferred from the parents to the QTLs detected increased grain yield. Most QTLs were detected for days to flowering. Three QTLs were located on chromosomes 10 and 4 (two QTLs) for days to flowering. qLDF-4a and qLDF-4b had a negative additive effect and the parent alleles of the mutant landrace Tarom reduced the number of days to flowering. These QTLs explained 11.6% of the phenotypic variance. Since the population under study was derived from a cross between landrace and mutant Tarom cultivars and the resulting population varied only in the mutated genes; so, the QTLs detected in this study were more accurate in location and expression levels, and after validation of them, they could be recommended for marker assistant selection breeding programs.","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132216925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Alizadeh, A. Rezaizad, Mohammad Yazdandoost Hamedani, G. Shiresmaeili, Farshad Nasserghadimi, H. Khademhamzeh
{"title":"Investigation of genotype × environment interaction and seed yield stability of rapeseed genotypes in cold and mild cold regions of Iran","authors":"B. Alizadeh, A. Rezaizad, Mohammad Yazdandoost Hamedani, G. Shiresmaeili, Farshad Nasserghadimi, H. Khademhamzeh","doi":"10.52547/pgr.7.2.6","DOIUrl":"https://doi.org/10.52547/pgr.7.2.6","url":null,"abstract":"","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127678297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic analysis of Biochemical and Physiological Traits using Haymen’s Graphical Approach in Lines and F2 Progenies of Maize (Zea mays L.)","authors":"M. Rahimi","doi":"10.52547/pgr.7.2.1","DOIUrl":"https://doi.org/10.52547/pgr.7.2.1","url":null,"abstract":"","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133378799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Karimizadeh, T. Hosseinpour, Jabbar Alt Jafarby, Kamal Shahbazi Homonlo, M. Armion
{"title":"Evaluation of Genotype × Environment Interaction and Determining Grain Yield Stability of Durum Wheat Genotypes in Uniform Regional Yield Trials in\u0000Semi-Warm Rainfed Areas","authors":"R. Karimizadeh, T. Hosseinpour, Jabbar Alt Jafarby, Kamal Shahbazi Homonlo, M. Armion","doi":"10.52547/pgr.7.2.3","DOIUrl":"https://doi.org/10.52547/pgr.7.2.3","url":null,"abstract":"","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"214 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124217516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}