Plant Genetic Researches最新文献

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Bioinformatics Study and Investigation of the Expression Pattern of Several Important Genes Involved in Glycyrrhizin Synthesis of Glycyrrhiza glabra L. in Autumn and Spring Seasons 秋、春季glyglyrhiza glabra L.合成甘草酸几个重要基因表达模式的生物信息学研究与研究
Plant Genetic Researches Pub Date : 2019-09-01 DOI: 10.29252/PGR.6.1.55
Atefeh Khakpour, M. Zolfaghari, K. Sorkheh
{"title":"Bioinformatics Study and Investigation of the Expression Pattern of Several Important Genes Involved in Glycyrrhizin Synthesis of Glycyrrhiza glabra L. in Autumn and Spring Seasons","authors":"Atefeh Khakpour, M. Zolfaghari, K. Sorkheh","doi":"10.29252/PGR.6.1.55","DOIUrl":"https://doi.org/10.29252/PGR.6.1.55","url":null,"abstract":"Glycyrrhiza is one of the important medicinal plants that is in danger of extinction. Search for finding accessions that have a higher glycyrrhizic acid is very important in breeding programs. Functional genomics methods such as EST sequencing prepare the ability to identify consensus gene families among studied species and interpretation of the genome. In this research, 55960 EST sequences of two different species of this plant along with the protein sequences were analyzed in order to identify the molecular aspects and functional analysis of the genome and the gene network involved in the biosynthesis of glycyrrhizin. Also, in order to validation of results, relative expression of four important genes in the pathway of glycyrrhizin biosynthesis including squalene synthase (SQS), β-amyrin synthase (BAS), β–amyrin 11-oxidase (CYP88D6) and UDP-glucuronyl transferase (UGT) were evaluated. After trimming and qualitative evaluation the sequences, 6427 contig sequences and 30895 singleton (37322 unigene) were generated, which covered a total of 26884666 bp (7.06%) of the licorice genome. Genome functional activity showed that most genes play a role in the catalytic activity and cellular and metabolic processes in which these genes interact within cells and intracellular organels. Locating this group of genes showed that the genes involved in glycyrrhizin biosynthesis pathway were localized in endoplasmic reticulum. Results of validation using qRT-PCR showed that in the autumn and in the rhizome tissue, the genes of BAS, CYP88D6, UGT and SQS were up-regulated. The results of this study can be valuable for genomic sequencing, functional groups, genetic diversity and functional genomics of this plant.","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127734531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Expression and antimicrobial activity analysis of dermaseptin B1 recombinant peptides in tobacco transgenic plants 烟草皮菌素B1重组肽的表达及抗菌活性分析
Plant Genetic Researches Pub Date : 2019-09-01 DOI: 10.29252/pgr.6.1.139
M. Khademi, F. Nazarian-Firouzabadi
{"title":"Expression and antimicrobial activity analysis of dermaseptin B1 recombinant peptides in tobacco transgenic plants","authors":"M. Khademi, F. Nazarian-Firouzabadi","doi":"10.29252/pgr.6.1.139","DOIUrl":"https://doi.org/10.29252/pgr.6.1.139","url":null,"abstract":"","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122727579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Identification of Microsatellite Markers Linked with Genomic Regions Involved in Resistance to Basal Stem Rot Disease Isolates in Oily Sunflower (Helianthus annuus L.) under Controlled Conditions 控制条件下油葵(Helianthus annuus L.)基腐病抗性基因组相关区域的微卫星标记鉴定
Plant Genetic Researches Pub Date : 2019-03-01 DOI: 10.29252/pgr.5.2.1
Khadijeh Mousa Khalifani, Reza Darvishzadeh, M. Abrinbana, A. Nouri
{"title":"Identification of Microsatellite Markers Linked with Genomic Regions Involved in Resistance to Basal Stem Rot Disease Isolates in Oily Sunflower (Helianthus annuus L.) under Controlled Conditions","authors":"Khadijeh Mousa Khalifani, Reza Darvishzadeh, M. Abrinbana, A. Nouri","doi":"10.29252/pgr.5.2.1","DOIUrl":"https://doi.org/10.29252/pgr.5.2.1","url":null,"abstract":"","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121610448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic Variation Pattern of Iranian Wheat Landraces Based on ISSR Molecular Markers and Morphological Traits 基于ISSR分子标记和形态性状的伊朗小麦地方品种遗传变异模式
Plant Genetic Researches Pub Date : 2018-09-01 DOI: 10.29252/PGR.5.1.87
A. Salami, M. Pahlevani, Khalil Zenalinezhad, M. E. Moghaddam
{"title":"Genetic Variation Pattern of Iranian Wheat Landraces Based on ISSR Molecular Markers and Morphological Traits","authors":"A. Salami, M. Pahlevani, Khalil Zenalinezhad, M. E. Moghaddam","doi":"10.29252/PGR.5.1.87","DOIUrl":"https://doi.org/10.29252/PGR.5.1.87","url":null,"abstract":"","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"150 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133888714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Phylogenetic Analysis of some Iranian Chrysanthemum morifolium Cultivars, using Internal Transcribed Spacer (ITS) Molecular Markers 利用ITS分子标记分析伊朗部分菊花品种的系统发育
Plant Genetic Researches Pub Date : 2018-09-01 DOI: 10.29252/pgr.5.1.55
Mitra Shahbazi, F. Nazarian-Firouzabadi, O. Akbarpour
{"title":"Phylogenetic Analysis of some Iranian Chrysanthemum morifolium Cultivars, using Internal Transcribed Spacer (ITS) Molecular Markers","authors":"Mitra Shahbazi, F. Nazarian-Firouzabadi, O. Akbarpour","doi":"10.29252/pgr.5.1.55","DOIUrl":"https://doi.org/10.29252/pgr.5.1.55","url":null,"abstract":"","PeriodicalId":263750,"journal":{"name":"Plant Genetic Researches","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131084013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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