Basic Hepatology最新文献

{"title":"IDDF2019-ABS-0263 Androgen receptor promotes gastric carcinogenesis via upregulating the expression of cell cycle-related kinase","authors":"Xiaoxing Li, Meng-ke Chen, Xiao-yi Xu, Ren-Tsung Wang, Lixia Xu, Ning Zhang, Gui-jun Zhao","doi":"10.1136/GUTJNL-2019-IDDFABSTRACTS.104","DOIUrl":"https://doi.org/10.1136/GUTJNL-2019-IDDFABSTRACTS.104","url":null,"abstract":"Background Gastric cancer (GC) is a leading global health problem. In most areas of the world, the incidence rate of GC in males was 1.5- to 3- fold higher than that in females. The androgen receptor (AR) is an independent adverse prognostic factor in patients with GC. However, the mechanism of AR regulating the progression of GC remains unclear. The aim of this study is to determine the effect of AR on the progression of GC and the mechanism behind these effects, which will provide novel ideas for the treatment of GC. Methods The relative expression of AR were detected by semi-quantitative RT-PCR and real-time quantitative PCR. The functions of AR in GC were determined by colony formation experiment, transwell migration and invasion assay. The clinical data were obtained from the website cBioPortal and XENA database. The potential mechanism of AR in GC was searched by Gene Set Enrichment Analysis (GSEA) and DAVID. Chromatin immunoprecipitation (ChIP) and PCR were performed to explore the function of AR as a transcriptional factor. The xenograft mouse models in nude mice were used to verify the function of CCRK in vivo. Results The expression of AR was upregulated in 6/8 GC cell lines. Compared to adjacent tissues, it’s expression of GC was higher. Ectopic expression of AR promoted the colony-formation ability, migration and invasion of GC cells. In contrast, the knockdown of AR showed the opposite effects. Remarkably, we found that AR regulated the expression of cell cycle related kinase (CCRK) through transcriptional regulation. The AR-CCRK axis promoted GC development through phosphorylation of GSK3β and β-catenin. Furthermore, TCGA data revealed that high expression of AR or CCRK was related with poor prognosis of GC patients. The prognosis of patients with concurrent high expression of AR and CCRK was significantly worse than that of patients with low expression of both AR and CCRK. Conclusions The expression of CCRK is increased by AR in GC. Low expression levels of AR and CCRK are related to better prognosis in GC patients, suggesting they are also candidates as prognostic indicators in GC.","PeriodicalId":261851,"journal":{"name":"Basic Hepatology","volume":"-1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125046330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2019-ABS-0058 A meta-analysis on the effectiveness of nanoparticle-mediated hyperthermia against hepatocellular carcinoma","authors":"J. Janairo, Marianne Linley Sy","doi":"10.1136/GUTJNL-2019-IDDFABSTRACTS.85","DOIUrl":"https://doi.org/10.1136/GUTJNL-2019-IDDFABSTRACTS.85","url":null,"abstract":"Background Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. HCC treatment remains a challenge; wherein tumor size limits immediate surgical interventions. Moreover, the success of radiofrequency ablation-based treatment is likewise restricted by the number and size of tumor, operator dependent imaging techniques, and collateral damage to the normal cells surrounding the tumor. A promising experimental treatment modality for tumors that can potentially overcome some of these limitations is nanoparticle - mediated hyperthermia (NMH). Currently at the developmental stage, NMH usually utilizes gold or iron oxide nanoparticles to convert the radiofrequency or alternating magnetic field into heat that causes targeted cell death. This meta-analysis therefore aims to assess the potential effectiveness of gold and iron oxide NMH for HCC treatment. Methods A systematic literature search in Scopus, Pubmed, and Google Scholar were conducted to identify relevant studies in gold and iron oxide NMH for HCC cell lines published from 2008 - January 2019. A total of eight studies were included, wherein four studies used gold NMH, and the remaining four used iron oxide NMH. The outcome of interest is cell viability after irradiation in the presence of the nanoparticles. A pooled risk ratio with 95% confidence interval (CI) was calculated using a random-effects model. Results Both gold (RR 0.63; 95% CI [0.53, 0.75]; p Conclusions NMH using gold and iron oxide nanoparticles is effective in decreasing HCC cell viability. This meta-analysis unravels the promising potential of NMH as a new treatment modality against HCC. The results presented provide encouraging insights for the continuous development of NMH for cancer treatment. Considering that iron oxide is more cost-effective than gold nanoparticles, focusing on the development of iron oxide nanoparticles for this nanomedical technology may accelerate the development and clinical application of NMH.","PeriodicalId":261851,"journal":{"name":"Basic Hepatology","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114248128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDDF2019-ABS-0218 Evaluation on the protection effect of the vismisco in the liver damage induced by paracetamol in mice experiment","authors":"Nhung Bui Thi Quynh, Song Nguyen Van","doi":"10.1136/gutjnl-2019-iddfabstracts.99","DOIUrl":"https://doi.org/10.1136/gutjnl-2019-iddfabstracts.99","url":null,"abstract":"Background This study was conducted to evaluate the hepatoprotective and antioxidant effects of Vismisco (extracted from Vigna radiata (L.) Wilczek, Smilax glabra roxb, Scoparia dulcis L.) in the liver damage induced by paracetamol in mice experiment. Aim Study the hepatoprotective effect of Vismisco on the paracetamol-induced hepatotoxicity. Methods Swiss albino mice weighing 25 ± 2 gram were divided into three groups of ten animals; Group 1: oral distilled water of 0.2 ml/10g; Group 2: oral distilled water and take paracetamol 400 mg/kg; Group 3: oral Silymarin at the dose of 140 mg/kg/day and take paracetamol 400 mg/kg; Group 4: oral Vismisco at the dose of 0.6g/kg/day and take paracetamol 400 mg/kg; Group 5: oral Vismisco at the dose of 1.8g/kg/day and take paracetamol 400 mg/kg. Swiss albino mice were oral with a single dose of 400mg/kg paracetamol to induce toxicity, while Vismisco administered in a dose of 0.6g/kg/day and 1.8g/kg/day. Animals were treated daily by the oral route of administration one a day in the morning for successive 8 days and observed once daily. On the 8th day after taking 2 hours of reagent, mice were given oral paracetamol dose of 400 mg/kg. Mice were sacrificed 48h after paracetamol oral to determine serum ALT, AST hepatic content of malonyl dialdehyde (MDA) and liver histopathology. Results The 8 days pretreatment of Vismisco at the oral dose of 0,6g/kg and 1,8g/kg increases the detoxified function of the liver and reduces the increasing level of AST, ALT reduces the increasing level of hepatic MDA, reduced the inflammation, hepatocellular necrosis which was induced by paracetamol. Conclusions Vismisco has the hepatoprotective effect from oxidative damages induced by reducing the generation of free radicals in the metabolic process of paracetamol, interrupt the lipid peroxidation of cellular membranes.","PeriodicalId":261851,"journal":{"name":"Basic Hepatology","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117134195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}