IDDF2019-ABS-0218 Evaluation on the protection effect of the vismisco in the liver damage induced by paracetamol in mice experiment

Abstract

Background This study was conducted to evaluate the hepatoprotective and antioxidant effects of Vismisco (extracted from Vigna radiata (L.) Wilczek, Smilax glabra roxb, Scoparia dulcis L.) in the liver damage induced by paracetamol in mice experiment. Aim Study the hepatoprotective effect of Vismisco on the paracetamol-induced hepatotoxicity. Methods Swiss albino mice weighing 25 ± 2 gram were divided into three groups of ten animals; Group 1: oral distilled water of 0.2 ml/10g; Group 2: oral distilled water and take paracetamol 400 mg/kg; Group 3: oral Silymarin at the dose of 140 mg/kg/day and take paracetamol 400 mg/kg; Group 4: oral Vismisco at the dose of 0.6g/kg/day and take paracetamol 400 mg/kg; Group 5: oral Vismisco at the dose of 1.8g/kg/day and take paracetamol 400 mg/kg. Swiss albino mice were oral with a single dose of 400mg/kg paracetamol to induce toxicity, while Vismisco administered in a dose of 0.6g/kg/day and 1.8g/kg/day. Animals were treated daily by the oral route of administration one a day in the morning for successive 8 days and observed once daily. On the 8th day after taking 2 hours of reagent, mice were given oral paracetamol dose of 400 mg/kg. Mice were sacrificed 48h after paracetamol oral to determine serum ALT, AST hepatic content of malonyl dialdehyde (MDA) and liver histopathology. Results The 8 days pretreatment of Vismisco at the oral dose of 0,6g/kg and 1,8g/kg increases the detoxified function of the liver and reduces the increasing level of AST, ALT reduces the increasing level of hepatic MDA, reduced the inflammation, hepatocellular necrosis which was induced by paracetamol. Conclusions Vismisco has the hepatoprotective effect from oxidative damages induced by reducing the generation of free radicals in the metabolic process of paracetamol, interrupt the lipid peroxidation of cellular membranes.