Reproductive and developmental Biology最新文献

Biochemical Characterization of 20α-Hydroxysteroid Dehydrogenase 20α-羟基类固醇脱氢酶的生化表征

Reproductive and developmental Biology Pub Date : 2018-05-30 DOI: 10.12749/rdb.2018.42.2.7

Munkhzaya Byambaragchaa, K. Min

{"title":"Biochemical Characterization of 20α-Hydroxysteroid Dehydrogenase","authors":"Munkhzaya Byambaragchaa, K. Min","doi":"10.12749/rdb.2018.42.2.7","DOIUrl":"https://doi.org/10.12749/rdb.2018.42.2.7","url":null,"abstract":"In this review, we have tried to summarize the evidence and molecular characterization indicating that 20α-hydroxysteroid dehydrogenase (20α-HSD) is a group of the aldo-keto reductase (AKR) family, and it plays roles in the modulation and regulation of steroid hormones. This enzyme plays a critical role in the regulation of luteal function in female mammals. We have studied the molecular expression and regulation of 20α-HSD in cows, pigs, deer, and monkeys. The specific antibody against bovine 20α-HSD was generated in a rabbit immunized with the purified recombinant protein. The mRNA expression levels increased gradually throughout the estrous cycle, the highest being in the corpus luteum (CL) 1 stage. The mRNA was also specifically detected in the placental and ovarian tissues during pregnancy. The 20α-HSD protein was intensively localized in the large luteal cells and placental cytotrophoblast villus, glandular epithelial cells of the endometrium, syncytiotrophoblast of the placenta, the isthmus cells of the oviduct, and the basal part of the primary chorionic villi and chorionic stem villus of the placenta and large luteal cells of the CL in many mammalian species. Further studies are needed to determine the functional significance of the 20αHSD molecule during ovulation, pregnancy, and parturition. This article will review how fundamental information of these enzymes can be exploited for a better understanding of the reproductive organs during ovulation and pregnancy. (","PeriodicalId":257457,"journal":{"name":"Reproductive and developmental Biology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133620930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signal Transduction of Equine Follicle-Stimulating Hormone Receptor (eFSHR) by rec-eelFSHβ/α, Natural Porcine FSH, and Natural Human FSH rec-eelFSHβ/α、天然猪FSH和天然人FSH对马促卵泡激素受体(eFSHR)信号转导的影响

Reproductive and developmental Biology Pub Date : 2018-03-31 DOI: 10.12749/rdb.2018.42.1.1

Munkhzaya Byambaragchaa, Dae-Jung Kim, Myung-Hwa Kang, K. Min

{"title":"Signal Transduction of Equine Follicle-Stimulating Hormone Receptor (eFSHR) by rec-eelFSHβ/α, Natural Porcine FSH, and Natural Human FSH","authors":"Munkhzaya Byambaragchaa, Dae-Jung Kim, Myung-Hwa Kang, K. Min","doi":"10.12749/rdb.2018.42.1.1","DOIUrl":"https://doi.org/10.12749/rdb.2018.42.1.1","url":null,"abstract":"In this study, we analyzed signal transduction by equine follicle-stimulating hormone receptor (eFSHR) on stimulation with recombinant eelFSHβ/α (rec-eelFSHβ/α), natural porcine FSH (pFSH), and natural human FSH (hFSH). cAMP stimulation in CHO-K1 cells expressing eFSHR was determined upon exposure to different doses (0-1450 ng/mL) of these hormones. The EC50 value of rec-eelFSHβ/α was 53.35 ng/mL. The Rmax values of rec-eelFSHβ/α and pFSH were 28.12 and 2.88 ng/mL, respectively. The activity of rec-eelFSHβ/α was much higher than that of natural pFSH. However, signal transduction in CHO PathHunter Parental cells expressing eFSHR was not enhanced by stimulation with natural hFSH. Thus, rec-eelFSHβ/α was completely active in cells expressing eFSHR. However, natural hFSH did not invoke a signal response in cells expressing eFSHR. Particularly, natural pFSH was weakly active in the same cells. These results showed that eelFSHβ/α has potent activity in cells expressing eFSHR. Thus, rec-eelFSHβ/α may efficiently bind to eFSHR, where as natural hFSH does not bind to eFSHR.","PeriodicalId":257457,"journal":{"name":"Reproductive and developmental Biology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125515121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant Effect of Alpha-Linolenic Acid during In Vitro Maturation in Porcine Oocytes α -亚麻酸在猪卵母细胞体外成熟过程中的抗氧化作用

Reproductive and developmental Biology Pub Date : 2017-12-31 DOI: 10.12749/RDB.2017.41.4.65

Jieun Lee, Yong Hwangbo, Hwa-young Kim, H. Cheong, B. Yang, Choon-keun Park

引用次数: 1

Evaluation of Cytotoxicity for Immunity Rejection of US11, hDAF US11、hDAF免疫排斥反应的细胞毒性评价

Reproductive and developmental Biology Pub Date : 2017-09-30 DOI: 10.12749/RDB.2017.41.3.57

J. Kang, H. Shin, Reza K. Oqani, T.-A. Lin, J. Lee, So Yeon Kim, J. Lee, D. Jin

{"title":"Evaluation of Cytotoxicity for Immunity Rejection of US11, hDAF","authors":"J. Kang, H. Shin, Reza K. Oqani, T.-A. Lin, J. Lee, So Yeon Kim, J. Lee, D. Jin","doi":"10.12749/RDB.2017.41.3.57","DOIUrl":"https://doi.org/10.12749/RDB.2017.41.3.57","url":null,"abstract":"Xenotransplantation is proposed as a solution to the problem of organ shortage. However, transplantation of xenogeneic organs induces an antigen-antibody reaction in α-1,3-gal structure that are not present in humans and primates, and thus complement is also activated and organs die within minutes or hours. In this study, we used FasL gene, which is involved in the immune response of NK cell, and US11, which suppresses MHC Class I cell membrane surface expression, to inhibit cell mediated rejection in the interspecific immunity rejection, and also hDAF(CD55) was introduced to confirm the response to C3 complement. These genes were tranfeced into Korean native pig fetal fibroblasts using pCAGGS vector. And cytotoxicity of NK cell and human complement was confirmed in each cell line. The US11 inhibited the cytotoxicity of NK cell and, in addition, the simultaneous expression of US11 and Fas ligand showed excellent suppress to T-lymphocyte cytotoxicity, hDAF showed weak resistance to cytotoxicity of natural killer cell but not in CD8+ CTLs. Cytotoxicity study with human complement showed that hDAF was effective for reducing complement reaction. In this studies have demonstrated that each gene is effective in reducing immune rejection.","PeriodicalId":257457,"journal":{"name":"Reproductive and developmental Biology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127347322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knock-in Vector for Expression of Insulin-like Growth Factor 1 on the Bovine β-casein Gene Locus 牛β-酪蛋白基因位点上表达胰岛素样生长因子1的敲入载体

Reproductive and developmental Biology Pub Date : 2017-09-30 DOI: 10.12749/RDB.2017.41.3.51

Sang Young Kim, Da Som Park, Se Eun Kim, M. Kang, D. Koo

引用次数: 0

X-Chromosome Inactivation: A Complex Circuits regulated x染色体失活:一个复杂的回路调控

Reproductive and developmental Biology Pub Date : 2017-06-30 DOI: 10.12749/RDB.2017.41.2.33

J. Hwang, Chang-Kyu Lee

引用次数: 0

Optimization of Estrus Synchronization Protocol for Target Breeding to 目标育种发情同步协议的优化

Reproductive and developmental Biology Pub Date : 2017-06-30 DOI: 10.12749/RDB.2017.41.2.25

Md. Parvez Kabir, M. Islam, A. Maruf, M. Shamsuddin, F. Bari, N. Juyena, S. Rahman

引用次数: 0

Methodologies for Cryopreservation of Mammalian Germline 哺乳动物生殖系冷冻保存方法

Reproductive and developmental Biology Pub Date : 2017-06-30 DOI: 10.12749/RDB.2017.41.2.41

P. C. Karmakar, Sang-Eun Jung, Buom-Yong Ryu

引用次数: 0

Knock-in Efficiency Depending on Homologous Arm Structure of the Knock-in Vector in the Bovine Fibroblasts 牛成纤维细胞中敲入蛋白载体同源臂结构的敲入效率

Reproductive and developmental Biology Pub Date : 2017-03-31 DOI: 10.12749/RDB.2017.41.1.7

Se Eun Kim, Da Som Park, D. Koo, M. Kang

{"title":"Knock-in Efficiency Depending on Homologous Arm Structure of the Knock-in Vector in the Bovine Fibroblasts","authors":"Se Eun Kim, Da Som Park, D. Koo, M. Kang","doi":"10.12749/RDB.2017.41.1.7","DOIUrl":"https://doi.org/10.12749/RDB.2017.41.1.7","url":null,"abstract":"The knock-in efficiency in the fibroblast is very important to produce transgenic domestic animal using nuclear transfer. In this research, we constructed three kinds of different knock-in vectors to study the efficiency of knock-in depending on structure of knock-in vector with different size of homologous arm on the β-casein gene locus in the somatic cells; DT-A_cEndo Knock-in vector, DT-A_tEndo Knock-in vector I, and DT-A_tEndo Knock-in vector II. The knock-in vector consists of 4.8 kb or 1.06 kb of 5’ arm region and 1.8 kb or 0.64 kb of 3’ arm region, and neomycin resistance gene(neor) as a positive selection marker gene. The cEndo Knock-in vector had 4.8 kb and 1.8 kb homologous arm. The tEndo Knock-in vector I had 1.06 kb and 0.64 kb homologous arm and tEndo Knock-in vector II had 1.06 kb and 1.8 kb homologous arm. To express endostatin gene as transgene, the F2A sequence was fused to the 5’ terminal of endostatin gene and inserted into exon 7 of the β-casein gene. The knock-in vector and TALEN were introduced into the bovine fibroblast by electroporation. The knock-in efficiencies of cEndo, tEndo I, and tEndo II vector were 4.6%, 2.2% and 4.8%, respectively. These results indicated that size of 3’ arm in the knock-in vector is important for TALEN-mediated homologous recombination in the fibroblast. In conclusion, our knock-in system may help to create transgenic dairy cattle expressing human endostatin protein via the endogenous expression system of the bovine β-casein gene in the mammary gland.","PeriodicalId":257457,"journal":{"name":"Reproductive and developmental Biology","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127031862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Studies on Steroid Hormone Concentration during the Estrous Cycle in the MediKinetics Micropig MediKinetics微猪发情周期中类固醇激素浓度的研究

Reproductive and developmental Biology Pub Date : 2017-03-31 DOI: 10.12749/RDB.2017.41.1.1

Hun Seong, Kyeong-Seok Seo, Jeong-Su Kim, Chang-Gi Her, Myung-Hwa Kang, Bo-Woong Sim, Jong-Taek Yoon, K. Min

引用次数: 1