Resolution and Discovery最新文献

{"title":"Quantitative dSTORM superresolution microscopy","authors":"Tibor Novák, Dániel Varga, P. Biró, B. B. H. Kovács, Hajnalka Majoros, T. Pankotai, Szilárd Szikora, J. Mihály, M. Erdélyi","doi":"10.1556/2051.2022.00093","DOIUrl":"https://doi.org/10.1556/2051.2022.00093","url":null,"abstract":"Localization based superresolution technique provides the highest spatial resolution in optical microscopy. The final image is formed by the precise localization of individual fluorescent dyes, therefore the quantification of the collected data requires special protocols, algorithms and validation processes. The effects of labelling density and structured background on the final image quality were studied theoretically using the TestSTORM simulator. It was shown that system parameters affect the morphology of the final reconstructed image in different ways and the accuracy of the imaging can be determined. Although theoretical studies help in the optimization procedure, the quantification of experimental data raises additional issues, since the ground truth data is unknown. Localization precision, linker length, sample drift and labelling density are the major factors that make quantitative data analysis difficult. Two examples (geometrical evaluation of sarcomere structures and counting the γH2AX molecules in DNA damage induced repair foci) have been presented to demonstrate the efficiency of quantitative evaluation experimentally.","PeriodicalId":251226,"journal":{"name":"Resolution and Discovery","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132575943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscopic and structural study on the formation of mechanochemical synthesized BaTiO3 and ZnTiO3 perovskites","authors":"G. Kozma, D. Berkesi, K. Lipták, Andrea Rónavári, Á. Kukovecz, Z. Kónya","doi":"10.1556/2051.2022.00092","DOIUrl":"https://doi.org/10.1556/2051.2022.00092","url":null,"abstract":"\u0000 In this work, the properties of mechanochemically produced (by using mills made from different materials) barium-titanate (BaTiO3) and zinc-titanate (ZnTiO3) perovskites are compared. Mechanochemistry is a process that can cover the energy demand of some reaction pathways between solid materials. This process is called “high-energy milling”, for which not all types of mills are suitable. In our case, a planetary ball mill provided the necessary energy. Using a model, the required energy is determinable; the energy released during an impact of a milling ball (E\u0000 \u0000 b\u0000 – ball-impact energy), as well as during the whole milling (E\u0000 cum – cumulative milling energy). Thus, a milling-energy map was created, with which the applied E\u0000 \u0000 b\u0000 and E\u0000 cum values were visualized depending on the different grinding parameters. The parameters changed were the material of the grinding vessels, the number of grinding balls, and the rotational speed. The transformation was tracked by X-ray diffraction (XRD) measurements, and electron microscopic images (TEM and SEM) of the perovskites produced were taken. This study aimed to draw conclusions that will help later in the synthesis of materials with other perovskite structures by choosing optimal milling parameters.","PeriodicalId":251226,"journal":{"name":"Resolution and Discovery","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117106255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visualization of the effect of TR100 anti-cancer compound on membrane nanotubes with SR-SIM microscopy","authors":"A. Hencz, Pál Somogyi, H. Halász, E. Szabó-Meleg","doi":"10.1556/2051.2022.00091","DOIUrl":"https://doi.org/10.1556/2051.2022.00091","url":null,"abstract":"\u0000 One of the most dangerous diseases is cancer, nearly 2 million new cancer types are diagnosed each year, worldwide, causing most of the death. Therefore, cancer is in the focus of many types of research. To prevent the proliferation and spreading of malignant cells, several compounds have been developed in chemotherapy, however, a significant proportion of these have serious side effects, and resistance is commonly emerging early after administration. Tumor cells require tropomyosin-containing actin network for their growth and survival. The tropomyosin profile is considerably changed in cancers resulting in the dramatic rearrangements of the actin cytoskeleton structure, therefore anti-tropomyosin compounds can be a new perspective in cancer therapy, such as TR100 which was reported to be capable of destroying cancer cells in a highly tumor-specific manner by increasing the depolymerization of the actin filament. On the other hand tumor cells can commonly communicate with each other via membrane nanotubes (NTs) for which actin is essential for growth. Tumor cell NTs may transport not only signal molecules, or cell organelles, but resistance factors against chemotherapeutic agents to help to survive. Immune cells also frequently use membrane nanotubes for communication, therefore, in this study we focused on the visualization of the effect of TR100 on the morphology and formation of B lymphoma cell NTs with superresolution structured illumination microscopy. TR100 treatment caused spectacular changes on the NT forming capability and the morphology of B cells in a concentration dependent manner, while low concentration of the agent significantly promoted NT formation, and at the same time produced shorter and thicker tubes in the early stage of their formation, in higher concentration it affected mainly only the cells, causing the rounding and finally the death of them. We were not able to detect any significant change on cells with extended nanotubular network, suggesting that TR100 is a less potent candidate in anti-cancer therapy.","PeriodicalId":251226,"journal":{"name":"Resolution and Discovery","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115782619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DiffMap: A new free computer program to process scanned electron diffraction patterns","authors":"J. Lábár","doi":"10.1556/2051.2022.00090","DOIUrl":"https://doi.org/10.1556/2051.2022.00090","url":null,"abstract":"\u0000 A free computer program, called DiffMap, is presented for off-line evaluation of both phase maps and orientation maps from a large number of diffraction patterns recorded with a nearly parallel nano-beam scanned line-by-line over a rectangular area in a scanning transmission electron microscope (STEM). The program runs in the Windows operating system on IBM PC compatible computers. The patterns, which are recorded independently from this program by a CCD or CMOS camera or by a pixelated camera are in Tif format, serve as input to DiffMap. Many STEMs can collect such a four-dimensional electron diffraction (4D-ED) data sets by proper selection of microscope parameters, even if this fact is not over-emphasized in the operating manuals. These phase and orientation maps can complement usual compositional maps collected in the same STEM with energy dispersive x-ray spectrometers (EDS) to give a complete description of the crystalline phases. Application is exemplified on the (fcc, hcp and bcc) phases in a sample with 4 major components (Co, Cr, Fe, Ni).","PeriodicalId":251226,"journal":{"name":"Resolution and Discovery","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121289003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact-formed complex diamond-graphite nanostructures","authors":"P. Németh, Kit McColl, L. Garvie, F. Corà, C. Salzmann, P. McMillan","doi":"10.1556/2051.2021.00089","DOIUrl":"https://doi.org/10.1556/2051.2021.00089","url":null,"abstract":"\u0000 Shock waves resulting from asteroidal and laboratory impacts convert sp2-bonded graphitic material to sp3-bonded diamond. Depending on the shock pressure and temperature conditions, complex nanostructures can form that are neither graphite nor diamond but belong to the diaphite material group, which are characterized by structurally intergrown layered sp2- and sp3-bonded carbon domains. Our ultrahigh-resolution transmission electron microscopy images combined with density functional theory calculations demonstrate that diaphites have two related but distinct structural families. Here, we describe diaphite nanostructures from natural and laboratory shocked samples, provide a framework for classifying the members of these materials, and draw attention to their excellent mechanical and electronic material properties.","PeriodicalId":251226,"journal":{"name":"Resolution and Discovery","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126211082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HRTEM image-stitching for measurement of distances","authors":"G. Radnóczi, Zoltán Herceg, Tamás Kiss","doi":"10.1556/2051.2020.00086","DOIUrl":"https://doi.org/10.1556/2051.2020.00086","url":null,"abstract":"Very accurate measurement of distances in the order of several µm is demonstrated on a single crystal Si sample by counting the lattice fringes on stitched high resolution TEM/STEM images. Stitching of TEM images commonly relies on correspondence points found in the image, however, the nearly perfect periodic nature of a lattice image renders such a procedure very unreliable. To overcome this difficulty artificial correspondence points are created on the sample using the electron beam. An accuracy better than 1% can be reached while measuring distances in the order of 1 µm. A detailed description of the process is provided, and its usability for accurately measuring large distances is discussed in detail.","PeriodicalId":251226,"journal":{"name":"Resolution and Discovery","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129283472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced graphene oxide-chitosan flexible nanocomposites for efficient bacteria capture and photothermal ablation","authors":"Milica Budimir, D. Kleut, B. Marković, R. Boukherroub","doi":"10.1556/2051.2020.00077","DOIUrl":"https://doi.org/10.1556/2051.2020.00077","url":null,"abstract":"One of the major public health concerns today is bacterial infection-associated diseases. Traditional antibacterial therapies are becoming less efficient because frequent and inadequate use of antibiotics has caused mutations in bacteria that led to many antibiotic-resistant bacterial strains. It is, therefore, crucial to develop novel antibacterial materials and strategies that will successfully combat both gram-positive and gram-negative bacteria. In the present study, we will demonstrate a simple and efficient method for bacteria capture and elimination through photothermal ablation. The developed material consists of a flexible Kapton substrate, coated with reduced graphene oxide-chitosan (rGO-CS) thin films. Reduced graphene oxide has strong absorption in the near-infrared (NIR) region, while chitosan has the ability to bind bacteria through electrostatic interactions. The K/rGO-CS device proved to capture and efficiently eradicate both planktonic Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacteria after 10 min of NIR (980 nm) irradiation.","PeriodicalId":251226,"journal":{"name":"Resolution and Discovery","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127129546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of graphene oxide and graphene quantum dots using nascent hydrogen: The investigation of morphological and structural changes","authors":"S. Jovanović, O. Haenssler, Milica Budimir, D. Kleut, J. Prekodravac, B. Marković","doi":"10.1556/2051.2020.00073","DOIUrl":"https://doi.org/10.1556/2051.2020.00073","url":null,"abstract":"In order to modify both chemical and electrical properties of graphene-based nanomaterials, we conducted the chemical modification of graphene oxide (GO) and graphene quantum dots (GQDs). The reaction of the reduction with nascent hydrogen was conducted on both materials. The structure and morphology of produced chemically reduced GO and GQDs were analyzed. While the chemical composition of both GQD and GO changed significantly, GO showed also significant changes in morphology as opposite to GQDs where were morphological changes were not observed.","PeriodicalId":251226,"journal":{"name":"Resolution and Discovery","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133562762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioceramic microspheres based on Si3N4–Ca3(PO4)2","authors":"T. Fenclová, Z. Jonšta, M. Hnatko, Josef Kraxner, P. Šajgalík","doi":"10.1556/2051.2018.00065","DOIUrl":"https://doi.org/10.1556/2051.2018.00065","url":null,"abstract":"This article describes a material prepared for biocements for medical use in human body. Biocements are used, for example, for bone implants or as a filling of bone cavities. The main method of pre...","PeriodicalId":251226,"journal":{"name":"Resolution and Discovery","volume":"107 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131956866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An economic and facile method for graphene oxide preparation from graphite powder","authors":"M. Furko, Z. Fogarassy, K. Balázsi, C. Balázsi","doi":"10.1556/2051.2019.00066","DOIUrl":"https://doi.org/10.1556/2051.2019.00066","url":null,"abstract":"A new method for preparing multi-layered graphene oxide powder was developed. In this method, the raw material was commercially available micro-sized graphite powder. The graphite powder was milled...","PeriodicalId":251226,"journal":{"name":"Resolution and Discovery","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123555889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}