ZooKeysPub Date : 2025-08-05eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1248.158182
Yu Fang, Cornelis van Achterberg, Pu Tang, Xue-Xin Chen
{"title":"Revision of genus <i>Zele</i> Curtis (Hymenoptera, Braconidae, Euphorinae) from China, with description of nineteen new species.","authors":"Yu Fang, Cornelis van Achterberg, Pu Tang, Xue-Xin Chen","doi":"10.3897/zookeys.1248.158182","DOIUrl":"10.3897/zookeys.1248.158182","url":null,"abstract":"<p><p><i>Zele</i> Curtis is a braconid parasitoid wasp genus within the subfamily Euphorinae (Hymenoptera, Braconidae), consisting of only 30 species worldwide. The Chinese species of the genus <i>Zele</i> are revised and 29 species are now recognised, including 19 species new to science: <i>Z.aquilus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.carinatus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.confusus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.cristatus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.curvatus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.curvinervis</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.densipunctatus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.extensus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.fulgidus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.fuscatus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.impolitus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.inclinator</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.irregularis</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.petiolatus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.rugulosus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.sculpticoxis</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.shaanxiensis</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, <i>Z.syntomus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b>, and <i>Z.vacatus</i> Fang, van Achterberg & Chen, <b>sp. nov.</b> 31 barcode region sequences of mitochondrial cytochrome c oxidase I (COI) from the genus <i>Zele</i> were obtained and combined with 102 sequences from BOLD. They were used to validate new species and to get an indication of similarity among species. Three species are reinstated: <i>Zeleperonatus</i> (Shestakov, 1940), <i>Z.romani</i> (Fahringer, 1929), and <i>Z.rufulus</i> (Thomson, 1895). In addition, an identification key for the <i>Zele</i> species recorded in China (plus one expected species) is provided.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1248 ","pages":"125-208"},"PeriodicalIF":1.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-08-04eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1248.159516
L Lee Grismer
{"title":"Introducing multiple factor analysis (MFA) as a diagnostic taxonomic tool complementing principal component analysis (PCA).","authors":"L Lee Grismer","doi":"10.3897/zookeys.1248.159516","DOIUrl":"10.3897/zookeys.1248.159516","url":null,"abstract":"<p><p>Multiple factor analysis (MFA) is introduced as a diagnostic tool for taxonomy and discussed using examples from the herpetological literature. Its methodology and output are compared and contrasted to the more often used principal component analysis (PCA). The most significant difference between MFA and PCA is that the former can more appropriately integrate numeric (meristic and/or morphometric) and categorical characters (e.g., big-small, blue-red, striped-banded, keeled-smooth, etc.) in the analysis, thus creating a nearly total-evidence morphological output. MFA emphasizes the diagnostic utility of categorical characters in a statistically defensible landscape as opposed to their often-anecdotal treatment or complete omission in species diagnoses, usually owing to their variability. PCA is most informative when only a single numeric data type (e.g., morphometric or meristic) is analyzed. Using PCA to analyze different data types separately and comparing the results, one can determine which data type and which of their variables (traits/characters) bear most heavily on the differentiation among the operational taxonomic units (OTUs [i.e., populations or species]) and, in some cases, their biological significance. If more than one data type is used in a PCA, the output may be biased by the data type with the largest amount of variation or statistical variance. Also discussed is the necessity of using a non-parametric permutation of analysis of variance (PERMANOVA)-or a similar analysis-as a robust, statistically defensible method for assessing the significance of OTU plot positions as opposed to subjective visual interpretations.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1248 ","pages":"93-109"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-08-04eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1248.153967
Jianshuang Zhang, Yuanqian Xing, Hao Yu, Shuqiang Li
{"title":"Six new species of the spider genus <i>Clubiona</i> Latreille, 1804 (Araneae, Clubionidae) from subtropical forests of Sichuan Province, China.","authors":"Jianshuang Zhang, Yuanqian Xing, Hao Yu, Shuqiang Li","doi":"10.3897/zookeys.1248.153967","DOIUrl":"10.3897/zookeys.1248.153967","url":null,"abstract":"<p><p>Six new species, belonging to three species groups of <i>Clubiona</i> Latreille, 1804 are described from both males and females: <i>C.huntianling</i> Yu & Li, <b>sp. nov.</b>, <i>C.rouqiu</i> Yu & Li, <b>sp. nov.</b> and <i>C.yinyangjian</i> Yu & Li, <b>sp. nov.</b> from the <i>corticalis</i> group; <i>C.huojianqiang</i> Yu & Li, <b>sp. nov.</b> and <i>C.qiankunquan</i> Yu & Li, <b>sp. nov.</b> from the <i>trivialis</i> group; <i>C.nezha</i> Yu & Li, <b>sp. nov.</b> from the <i>zilla</i> group. These species are currently known to occur only in subtropical forests, Sichuan, China. The DNA barcodes of all species were obtained for species delimitation, matching of sexes and future use.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1248 ","pages":"61-91"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-08-04eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1248.157257
Si-Xun Ge, Li-Li Ren, Jiang-Li Tan
{"title":"The mid-Cretaceous crown wasp genus †<i>Tumidistephanus</i> Ge & Tan: discovery of the first male and a new species (Hymenoptera, Stephanidae).","authors":"Si-Xun Ge, Li-Li Ren, Jiang-Li Tan","doi":"10.3897/zookeys.1248.157257","DOIUrl":"10.3897/zookeys.1248.157257","url":null,"abstract":"<p><p>The fossil crown wasp †<i>Tumidistephanusepimetheus</i> <b>sp. nov.</b> is described based on a well-preserved male specimen embedded in mid-Cretaceous Burmese amber. This discovery adds to the growing diversity of the extinct subfamily †Lagenostephaninae, representing its fourth known species. Notably, it constitutes only the second male specimen described within this lineage, offering valuable insight into the morphological characteristics and potential sexual dimorphism in early crown wasps.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1248 ","pages":"53-59"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-08-04eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1248.151641
Edith Avila-Treviño, Gabriel Fernando Cardoza-Martínez, Fernando Alonzo-Rojo, Rodolfo Pérez-Rodríguez
{"title":"<i>Ictalurusnazas</i> sp. nov., a new species of North American catfish (Siluriformes, Ictaluridae) from Mexico.","authors":"Edith Avila-Treviño, Gabriel Fernando Cardoza-Martínez, Fernando Alonzo-Rojo, Rodolfo Pérez-Rodríguez","doi":"10.3897/zookeys.1248.151641","DOIUrl":"10.3897/zookeys.1248.151641","url":null,"abstract":"<p><p>Catfishes of the genus <i>Ictalurus</i> (Ictaluridae) range geographically from southern Canada to northern Guatemala and Belize. The systematics of this genus remain unresolved, with recent studies suggesting the presence of cryptic diversity. This is the case for a potentially undescribed catfish species distributed in the Nazas River basin, which has been recorded to date as a population of <i>Ictaluruspricei</i>. Recent phylogenetic studies suggest that it represents an independently evolving lineage that is distinct from <i>I.pricei</i>. This study aimed to determine the diagnostic characters that differentiate the Nazas River basin lineage and to describe this lineage as a new taxon. Morphological comparisons were made using meristic and morphometric characters. The study identified useful meristic and morphometric characters for diagnosing <i>Ictalurusnazas</i> <b>sp. nov.</b>, which differentiate it from <i>I.pricei</i>.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1248 ","pages":"33-51"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-08-04eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1248.159542
María de Los Ángeles Gallardo Salamanca, Cynthia Asorey, Enrique Macpherson
{"title":"A new species of <i>Galathea</i> (Decapoda, Galatheidae) from the seamounts of the Easter Island area (Southeast Pacific Ocean Ridge) associated with a sea urchin.","authors":"María de Los Ángeles Gallardo Salamanca, Cynthia Asorey, Enrique Macpherson","doi":"10.3897/zookeys.1248.159542","DOIUrl":"10.3897/zookeys.1248.159542","url":null,"abstract":"<p><p><i>Galatheatukitukimea</i> <b>sp. nov.</b> is described from the seamounts near Rapa Nui (Easter Island) and represents the first record of the genus for this region of the Pacific Ocean and for Chilean territory. The new species belongs to the group of species having the carapace with median protogastric and cardiac spines. <i>G.tukitukimea</i> has always been observed associated with the sea urchin <i>Stereocidarisnascaensis</i>. This potential mimicry-based association is uncommon in squat lobsters, which warrants further study.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1248 ","pages":"111-123"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-08-04eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1248.158273
Chengzhi Dai, Qiang Chen, Yanfeng Tong, Dongju Bian, Shuqiang Li
{"title":"Two new species of <i>Trilacuna</i> Tong & Li, 2007 (Araneae, Oonopidae) from Yunnan Province, China, with a key to all known <i>Trilacuna</i> species of Yunnan.","authors":"Chengzhi Dai, Qiang Chen, Yanfeng Tong, Dongju Bian, Shuqiang Li","doi":"10.3897/zookeys.1248.158273","DOIUrl":"10.3897/zookeys.1248.158273","url":null,"abstract":"<p><p>Two new species of the genus <i>Trilacuna</i> Tong & Li, 2007, <i>T.manhao</i> Tong & Li, <b>sp. nov.</b> (♂♀) and <i>T.mopanshan</i> Tong & Li, <b>sp. nov.</b> (♂♀), are described from Yunnan, China. Descriptions, diagnoses, photomicroscopy images and a key to species of Yunnan Province are provided.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1248 ","pages":"17-32"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-08-04eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1248.150650
Zhong-Jing Wang, Yan-Bin Yao, Xiao-Ting Lyu, Ke-Ke Liu, Yu-Cheng Lin
{"title":"Two new species of Synotaxidae from eastern China (Araneae, Synotaxidae).","authors":"Zhong-Jing Wang, Yan-Bin Yao, Xiao-Ting Lyu, Ke-Ke Liu, Yu-Cheng Lin","doi":"10.3897/zookeys.1248.150650","DOIUrl":"10.3897/zookeys.1248.150650","url":null,"abstract":"<p><p>This paper describes two new synotaxid members from eastern China: the species <i>Hamusqingming</i> Yao, Liu & Lin, <b>sp. nov.</b> (♂, ♀, Fujian) and <i>Tekellinazhangziqingi</i> Yao, Liu & Lin, <b>sp. nov.</b> (♂, ♀, Jiangxi). Diagnoses, detailed descriptions, and illustrations are provided for both species. The geographic distributions of five Synotaxidae genera are discussed.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1248 ","pages":"1-16"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-07-31eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1247.137606
Christopher J Glasby, Olga Biriukova, Patrick Martin, Geoffrey R Dyne, Serge Utevsky, Robin S Wilson
{"title":"ANNiKEY Linear - diagnoses, descriptions, and a single-access identification key to Annelida family-level taxa.","authors":"Christopher J Glasby, Olga Biriukova, Patrick Martin, Geoffrey R Dyne, Serge Utevsky, Robin S Wilson","doi":"10.3897/zookeys.1247.137606","DOIUrl":"10.3897/zookeys.1247.137606","url":null,"abstract":"<p><p>Phylum Annelida are ubiquitous metazoans found in almost every terrestrial and aquatic habitat on Earth. Historically, taxonomic studies on the phylum have been focused largely on its majorgroups, polychaetes, oligochaetes and leeches, so that while family-level keys for each group are available, no single-source identification guide exists to the world's annelid families. Here, the first illustrated linear key to annelid families is provided and family-level descriptions and diagnoses that distinguish individuals of each family from those of other families in the phylum are updated. This information is generated from an annelid DELTA database of 334 characters and 166 mostly family-level taxa. A link is provided to downloadable software (ANNiKEY Interactive) allowing the same data to be interrogated using the open-source DELTA program Intkey, which enables both interactive identification and taxonomic query functionality. For each family-level taxon, a diagnosis, full description, links to taxonomic data at the World Register of Marine Species, illustrations of diagnostic features, and a summary of the recent literature, including a list of published keys to genera and species are provided.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1247 ","pages":"217-403"},"PeriodicalIF":1.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144849198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Establishing a <i>COI</i> DNA barcoding reference database for Taiwanese coastal fish species from egg and larvae specimens.","authors":"Kwang-Tsao Shao, Hui-Ling Ko, Yung-Chieh Chiu, Hsin-Kai Huang, Ya-Fang Chen, Yen-Wei Chang","doi":"10.3897/zookeys.1247.151795","DOIUrl":"10.3897/zookeys.1247.151795","url":null,"abstract":"<p><p>Fish eggs and larval stages are essential components of marine ecosystems and play important roles in sustaining marine food webs. However, the egg and larval stages often lack distinct diagnostic characteristics, making it challenging to identify species solely based on their morphology. In this study, we applied a DNA barcoding approach targeting the cytochrome <i>c</i> oxidase subunit I gene to establish a comprehensive reference for fish eggs and larval material in coastal waters off Taiwan. A total of 7602 records of fish eggs and larvae were collected from marine coastal waters surrounding Taiwan between 2004 and 2023 and identified using a DNA barcoding approach. By comparing our newly generated DNA barcoding sequences with records from public reference databases, we identified 1112 different fish taxa encompassing 24 orders, 158 families, 500 genera, and 844 fish species. This DNA barcoding referencing effort will facilitate future studies on the early life stages of Taiwanese coastal marine fish communities. In addition, it will also provide an important baseline for the development of new methods, such as eDNA and DNA metabarcoding diet analysis. This database provides comprehensive spatial and temporal distribution data of fish eggs and larvae in the waters surrounding Taiwan, contributing to a better understanding of fish spawning seasons, spawning grounds, and resource fluctuations. This database enhances the accuracy of species identification and serves as a scientific foundation for biodiversity conservation and fishery resource management.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1247 ","pages":"207-216"},"PeriodicalIF":1.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144800476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}