ZooKeysPub Date : 2025-09-19eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1252.148379
Yoko Matsumura
{"title":"Female and male genital surface microstructures in shining leaf beetles (Coleoptera, Chrysomelidae, Criocerinae).","authors":"Yoko Matsumura","doi":"10.3897/zookeys.1252.148379","DOIUrl":"10.3897/zookeys.1252.148379","url":null,"abstract":"<p><p>Female genital diversity and co-evolution of female and male genitalia have been increasingly unveiled, particularly through recent studies. These findings highlight the importance of genital coupling in genital studies. In this pilot study, I examined membranous elements of female and male genitalia that come into physical contact during copulation in five representative species of shining leaf beetles by employing stereomicroscopy and scanning electron microscopy. The female genital surfaces are largely smooth, but a thickened sensilla-bearing patch is present. In contrast, the male surfaces are at least partially covered with microprotrusions. I present distribution maps of these microstructures for all study species. By comparing the results with those of previous studies, I discuss their possible functions and future research directions in this field.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1252 ","pages":"151-165"},"PeriodicalIF":1.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12475920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-09-19eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1252.151737
Orlando A Calcetas, Joel L Adorada, Michael Schmitt, Caroline S Chaboo
{"title":"Construction of the fecal armor of larvae of <i>Podontia quatuordecimpunctata</i> (L.) (Chrysomelidae, Galerucinae, Alticini) and its role against insecticides in pest management.","authors":"Orlando A Calcetas, Joel L Adorada, Michael Schmitt, Caroline S Chaboo","doi":"10.3897/zookeys.1252.151737","DOIUrl":"10.3897/zookeys.1252.151737","url":null,"abstract":"<p><p>Five subfamilies within Chrysomelidae (leaf beetles) have larvae that retain their feces as a coat or armor which serves for thermoregulation, camouflage, or barrier to enemies. The construction, retention and repair of these fecal structures are associated with specialized larval morphologies in the tortoise beetles (subfamily Cassidinae) and in the Cryptocephalinae + Lamprosomatinae (Camptosomata), but morphology associated with fecal encrustations on larvae in the <i>Blepharida</i>-group flea beetles (Galerucinae: Alticini) and in Criocerinae have not been examined. Experiments with live larvae of <i>Podontia quatuordecimpunctata</i> (L., 1767) (or sineguelas leaf beetle, SLB; <i>Blepharida</i> group) reveal the anus opens dorsally and deposits feces directly to the larva's dorsum; the armor is maintained and is reconstructed. Scanning electron microscopy reveals integumental microtrichia that presumably hold on the feces. This invasive beetle has become an introduced tree-crop pest in the Philippines, so ongoing research seeks to mitigate its population. Insecticidal chemical assays show that fecal armor does not fully protect SLB larvae but delays potency slightly. The study recommends rotating the insecticides (Imidacloprid, Cypermethrin, and Buprofezin) to prevent the development of resistance. Specialized morphology for fecal retention is known in Cassidinae, Camptosomata and is now documented in the <i>Blepharida</i> group. Such morphology and the fecal-building behavior can offer additional phylogenetic information for these beetles.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1252 ","pages":"167-182"},"PeriodicalIF":1.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12475907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-09-19eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1252.149428
David G Furth, Ariel-Leib-Leonid Friedman, Tamar Feldstein-Farkash
{"title":"Rediscovery of a second species of Reed Beetle in Israel (Coleoptera, Chrysomelidae, Donaciinae).","authors":"David G Furth, Ariel-Leib-Leonid Friedman, Tamar Feldstein-Farkash","doi":"10.3897/zookeys.1252.149428","DOIUrl":"10.3897/zookeys.1252.149428","url":null,"abstract":"<p><p>A species of reed beetle, <i>Donacia simplex</i> (Coleoptera, Chrysomelidae, Donaciinae) was recently rediscovered in northern Israel. Now a second species, <i>Donacia tomentosa</i>, has been rediscovered (verified using molecular analysis) living further south in Israel. In 2021 a reed beetle expert discovered a very old record of <i>Donacia tomentosa</i> from modern day Israel in the collections of the Natural History Museum (London) from a few specimens collected near Tel Aviv by the British in 1918 during WWI. In 2022 the authors discovered a viable population of <i>D. tomentosa</i> in Israel. Given the extensive amount of collecting by many good field entomologists in that region since 1918, it is surprising that this species had not been seen for 104 years. The location, biology, molecular analysis, and other details of this newly found viable population of <i>D. tomentosa</i> in Israel are provided here.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1252 ","pages":"77-96"},"PeriodicalIF":1.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12475923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-09-19eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1252.144951
Cibele Stramare Ribeiro-Costa, Geoffrey E Morse
{"title":"Circumscription of the <i>sclerolobii</i> group of <i>Amblycerus</i> Thunberg (Coleoptera, Chrysomelidae, Bruchinae) with descriptions of four new species and a revision of the species groups of the genus.","authors":"Cibele Stramare Ribeiro-Costa, Geoffrey E Morse","doi":"10.3897/zookeys.1252.144951","DOIUrl":"10.3897/zookeys.1252.144951","url":null,"abstract":"<p><p>The seed-beetle <i>Amblycerus</i> Thunberg, 1815 the second most diverse genus of Bruchinae (Chrysomelidae) in the Western Hemisphere, is organized into groups of species, as many others in the subfamily. The main objective of this work is to delimit the group <i>sclerolobii</i>, here established as composed by five described species, <i>A. sclerolobii</i> Ribeiro-Costa, 2000, <i>A. kingsolveri</i> Ribeiro-Costa, 1993, <i>A. marinonii</i> Ribeiro-Costa, 1993, <i>A. manauara</i> Ribeiro-Costa, 2000, <i>A. tachigaliae</i> Kingsolver, 1976 and four new species: <i>A. biacutus</i> Ribeiro-Costa, <b>sp. nov.</b>, <i>A. falcorostrus</i> Ribeiro-Costa & Morse, <b>sp. nov</b>., <i>A. morsei</i> Ribeiro-Costa, <b>sp. nov.</b> (type localities: Brazil: Amazonas, Manaus) and <i>A. truncatus</i> Ribeiro-Costa, <b>sp. nov.</b> (type locality Brazil: Mato Grosso, Sinop). Therefore, including these new species 70 Brazilian species of <i>Amblycerus</i> were recorded. <i>Amblycerus bicolor</i> (Pic, 1927) previously considered to belong to this group is excluded and placed as incertae sedis. The <i>sclerolobii</i> group is distributed mainly in the Brazilian Amazonia biome, consuming <i>Tachigali</i> Aubl., <i>Dinizia</i> Ducke, and <i>Stryphnodendron</i> Mart. seeds (Fabaceae). Diagnoses are presented for this group and for ten species (including <i>A. bicolor</i>), and descriptions for the four new ones, an identification key for the nine species, colored photographs of external morphology, and detailed male genitalia illustrations for all the species studied. Due mainly to previously broad contributions on the taxonomy of <i>Amblycerus</i> that resulted in many species groups, we also present a revised study of this intrageneric taxonomic level. From 37 species groups now we update to 32 groups with a total of 96 species assigned to them. Finally, a detailed systematic study of some specific groups is also suggested before mergence.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1252 ","pages":"35-67"},"PeriodicalIF":1.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12475921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-09-19eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1252.146087
Takuya Takemoto, Andrzej O Bieńkowski, Satoru Saitoh
{"title":"On the type specimens of representatives of the subgenus Apterosoma Motschulsky, 1861 of the genus <i>Chrysolina</i> Motschulsky, 1861 (Coleoptera, Chrysomelidae).","authors":"Takuya Takemoto, Andrzej O Bieńkowski, Satoru Saitoh","doi":"10.3897/zookeys.1252.146087","DOIUrl":"https://doi.org/10.3897/zookeys.1252.146087","url":null,"abstract":"<p><p>Classification of the subgenus Apterosoma Motschulsky of the genus <i>Chrysolina</i> is fraught with several problems. In particular, the northern Japanese <i>Apterosoma</i> can be separated into at least 20 morphologically and/or genetically distinct, yet undescribed, \"units\". In this study, we examine type specimens of the three species included within the subgenus to determine their relationships to these units and to each other.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1252 ","pages":"97-110"},"PeriodicalIF":1.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12475927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-09-19eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1252.142681
Zheng-Zhong Huang, Chao-Fan Zhang, Shi-Xing Leng, Xing-Ke Yang, Si-Qin Ge
{"title":"First record of the genus <i>Macrispa</i> in China, with a redescription of <i>Macrispa saundersii</i> Baly, 1859 (Chrysomelidae, Cassidinae, Botryonopini).","authors":"Zheng-Zhong Huang, Chao-Fan Zhang, Shi-Xing Leng, Xing-Ke Yang, Si-Qin Ge","doi":"10.3897/zookeys.1252.142681","DOIUrl":"https://doi.org/10.3897/zookeys.1252.142681","url":null,"abstract":"<p><p>The genus <i>Macrispa</i> Baly, 1859, previously known only from Southeast Asia and south of the Himalayas, has remained unrecorded in China until now. Here, we report the first discovery of <i>Macrispa saundersii</i> Baly, 1859 from Xizang Autonomous Region, China, representing a northward range extension for the genus. Through integrative examination of external morphology and male genitalia, we provide a comprehensive redescription of <i>M. saundersii</i>. High-resolution stacked imaging was employed to document key taxonomic characters, including elytral punctation patterns, stridulatory files, abdominal ventrite structures, and male genitalia.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1252 ","pages":"69-76"},"PeriodicalIF":1.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12475924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-09-19eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1252.150709
Kunio Suzuki, Haruo Takizawa
{"title":"Outline of the history and status of the Japanese Chrysomelidae (Coleoptera).","authors":"Kunio Suzuki, Haruo Takizawa","doi":"10.3897/zookeys.1252.150709","DOIUrl":"10.3897/zookeys.1252.150709","url":null,"abstract":"<p><p>The status of the Japanese fauna of the family Chrysomelidae is reviewed from the viewpoints of systematics and zoogeography based on a new systematic catalog in which 648 species belonging to 172 genera in 17 subfamilies were compiled by Takizawa and Suzuki. The rich Japanese chrysomelid fauna reflects a remarkable diversity of its natural environments. The number of species is relatively high in five subfamilies: Alticinae, Galerucinae, Eumolpinae, Chrysomelinae, and Cryptocephalinae. The percentage of indigenous species (275) compared with the total number of species exceeds 42.4%. The local faunas are compared among 10 regions. The history of studies on the Japanese Chrysomelidae is presented in three stages, Stage I (1758-1850), Stage II (1851-1950), and Stage III (1951-2023). Changes in number of species are presented for every 10 years period from 1758 to 2023. The cumulative number of species in the whole family clearly demonstrates a unique pattern with two remarkable increase times. We chose 10 representative coleopterists who contributed much to the elucidation of the Japanese chrysomelid fauna.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1252 ","pages":"219-233"},"PeriodicalIF":1.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12475905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-09-15eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1251.160506
Yong Zhou, Jie Yan, Bin Chen
{"title":"<i>Tuberstira pennis</i> (Coleoptera, Tenebrionidae, Lagriinae), a bizarre new genus and species from South China.","authors":"Yong Zhou, Jie Yan, Bin Chen","doi":"10.3897/zookeys.1251.160506","DOIUrl":"10.3897/zookeys.1251.160506","url":null,"abstract":"<p><p><i>Tuberstira pennis</i> <b>gen. et sp. nov.</b>, a new genus and species within the tribe Lagriini, subtribe Statirina, is described from Guangdong, Guangxi, Yunnan and Hainan, China. The genus is readily distinguished from all known Lagriini genera by: large eyes, nearly contiguous on the ventral surface; centrally elevated pronotal disc; corrugated elytra, with setigerous tubercles in intervals; coarsely punctate femora; and transversely expanded aedeagal apex in lateral view. The habitus, male genitalia, female genital tube, ovipositor and key diagnostic features are illustrated.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1251 ","pages":"353-362"},"PeriodicalIF":1.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-09-15eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1251.160858
Kyuhee Cho, Jong Guk Kim, Jimin Lee
{"title":"Discovery of Laophontidae (Copepoda, Harpacticoida) from marine plastic debris: <i>Pseudonychocamptus setadefectus</i> sp. nov. and <i>Heterolaophonte discophora</i> (Willey, 1929).","authors":"Kyuhee Cho, Jong Guk Kim, Jimin Lee","doi":"10.3897/zookeys.1251.160858","DOIUrl":"10.3897/zookeys.1251.160858","url":null,"abstract":"<p><p>Two species belonging to the family Laophontidae Scott T., 1905 were identified among harpacticoid copepods collected from marine plastic debris (MPD) stranded along the Korean coastline. These species were assigned to the genera <i>Pseudonychocamptus</i> Lang, 1944 and <i>Heterolaophonte</i> Lang, 1948. <i>Pseudonychocamptus setadefectus</i> <b>sp. nov.</b> conforms to the generic diagnosis, displaying sexual dimorphism in the armature of the swimming legs. It is distinguished from its six congeners by possessing only two endites on the maxillary syncoxa, notably lacking the proximal endite bearing the seta that is present in all other known species within the genus. Although most morphologically similar to <i>P. colomboi</i> Ceccherelli, 1988, the new species differs in several key characteristics, including the number of setae on the maxillary endopod, the relative length ratios of setae on the female P5 exopod, the presence of an inner seta on the male P5 exopod, and the asymmetry of the male P6. The second species, <i>Heterolaophonte discophora</i> (Willey, 1929), was previously reported from the Atlantic coast of Canada and the Pacific coasts of the USA and Japan. The Korean specimens closely correspond to these previous records, yet exhibit intraspecific variation, particularly in the setal count on the female P3 enp-2. Additional morphological differences were also observed, including variations in the segmentation of the male P3 endopod and in the number of setae on the maxillary endopod. Based on the sexually dimorphic traits observed in males, we propose the subdivision of the genus <i>Heterolaophonte</i> into four distinct species groups. The discovery of <i>P. setadefectus</i> <b>sp. nov.</b> and <i>H. discophora</i> on anthropogenic MPD underscores the potential role of such substrates as microhabitats for benthic harpacticoid copepods.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1251 ","pages":"323-352"},"PeriodicalIF":1.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZooKeysPub Date : 2025-09-15eCollection Date: 2025-01-01DOI: 10.3897/zookeys.1251.157817
Tan Van Nguyen, Nikolay A Poyarkov, Gernot Vogel
{"title":"Taxonomic reassessment of the <i>Lycodon rufozonatus</i> species complex (Serpentes, Colubridae), with re-evaluation of <i>Dinodon rufozonatum walli</i>, and description of a new species from north-central Vietnam.","authors":"Tan Van Nguyen, Nikolay A Poyarkov, Gernot Vogel","doi":"10.3897/zookeys.1251.157817","DOIUrl":"10.3897/zookeys.1251.157817","url":null,"abstract":"<p><p>The Red-banded Wolf Snake, <i>Lycodon rufozonatus</i> Cantor, 1842 has a complex taxonomic history. In this study, an integrative taxonomic approach is applied, incorporating morphological analyses, cytochrome <i>b</i> mitochondrial gene sequencing, and a re-examination of available type material to clarify the longstanding taxonomic uncertainties within <i>Lycodon rufozonatus</i> species complex. Our findings restrict the distribution of <i>Lycodon rufozonatus</i> to mainland China, Taiwan, the Korean Peninsula, Russia (southern Primorsky Krai), Japan (Tsushima Islands, Nagasaki), and northeastern Vietnam. Additionally, <i>Dinodon rufozonatum walli</i> Stejneger, 1907, previously considered a subspecies, is elevated to full species status as <i>Lycodon walli</i> <b>stat. nov.</b>, endemic to the Miyako and Yaeyama Islands, Okinawa, Japan. Furthermore, a new cryptic species is described from north-central Vietnam, <i>Lycodon duytan</i> <b>sp. nov.</b>, which is distinguished from <i>L. rufozonatus</i> and <i>L. walli</i> <b>stat. nov.</b> by differences in body scalation, colouration, and the number of keeled dorsal scales. Our findings highlight the underestimated diversity within <i>Lycodon</i> and contribute to a more refined understanding of its taxonomy. This study increases the total number of recognised <i>Lycodon</i> species to 78, underscoring the importance of integrative approaches in resolving taxonomic complexities within the genus.</p>","PeriodicalId":24051,"journal":{"name":"ZooKeys","volume":"1251 ","pages":"293-322"},"PeriodicalIF":1.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}