Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue最新文献

{"title":"[Early treatment of atelectasis by bronchoscopy in craniotomy patients].","authors":"Yan-ping Qiu,&nbsp;Yong-ju Chen,&nbsp;Rong-rong Song,&nbsp;Gang Wang,&nbsp;Jian-jun Guan,&nbsp;Hao-liang Ni,&nbsp;Yong Ji","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the safety and effects of early bronchoscopy on atelectasis of the ventilation patients, whom had experienced craniotomy for severe cranial trauma and hemorrhage.</p><p><strong>Methods: </strong>Fifty-five patients suffered from severe cranial trauma and hemorrhage with Glascow coma scores (GCS) less than 8 complicated by atelectasis after craniotomy were early given sputum suction by bronchoscope via extratracheal intubation and broncho-alveolar lavage (BAL) during tracheal intubation and mechanical ventilation. During the treatment, patients' consciousness, vital signs and arterial blood gas were closely monitored. The relevant data, before, during (5, 10, and 25 minutes), bronchoscopy treatment completed and 30 minutes after bronchoscopy, were recorded and analyzed.</p><p><strong>Results: </strong>Eighty-two time of bronchoscopies and 111 time of local BALs in 55 patients were completed and were effective for atelectasis. The patient's GCS (5.6±2.5 vs. 5.4±2.6, P>0.05), heart rate (HR), respiratory rate (RR), systolic blood pressure (SBP), blood oxygenous saturation (SaO(2)) were not deteriorated during bronchoscopy. Compared with pre-bronchoscopy, the HR and SBP decreased (HR: 88.2±14.2 bpm vs. 98.2±18.3 bpm, SBP: 110.6±18.2 mm Hg vs. 118.4±18.5 mm Hg, both P<0.05), and SaO(2) increased (0.982±0.022 vs. 0.945±0.035, P<0.05), pH, arterial partial pressure of oxygen (PaO(2)) and arterial partial pressure of carbon dioxide (PaCO(2)) had no significant changes during bronchoscopy. There was obviously increased in PaO(2) (84.5±14.4 mm Hg, 81.6±18.2 mm Hg vs. 76.2±15.4 mm Hg, both P<0.05), and decreased in PaCO(2) (27.0±12.8 mm Hg, 29.3±18.2 mm Hg vs. 36.5±11.6 mm Hg, both P<0.05) respectively, significantly decreased in alveolar arterial pressure of oxygen difference [P ((A-a))O(2)] at 10 minutes and 25 minutes, and at the time bronchoscopy treatment completed and the time 30 minutes after compared with before bronchoscopy (36.1±4.7 mm Hg, 32.4±6.2 mm Hg, 32.5±5.2 mm Hg, 31.2±7.2 mm Hg vs. 38.5±5.6 mm Hg, all P<0.05). All patients had not encounter side effects related with bronchoscopy and ventilation.</p><p><strong>Conclusion: </strong>The bronchoscope via extratracheal intubation for sputum suction and BAL were safe and effective treatment to the patients suffered from severe cranial trauma or hemorrhage complicated by atelectasis after craniotomy during mechanical ventilation, without obvious changes of the vital signs.</p>","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"24 12","pages":"759-62"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31061968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Correlation between osteoprotegerin gene 209G/A, 245T/G and 1181G/C polymorphism and acute coronary syndromes in Han people of Fujian area].","authors":"Zhu-ron Luo,&nbsp;Qin-jing Xie","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To explore the association of osteoprotegerin (OPG) gene polymorphisms 209G/A, 245T/G and 1181G/C in the Han population of Fujian province with acute coronary syndrome (ACS) and its severity.</p><p><strong>Methods: </strong>A prospective study was conducted. Two hundred and fifty-two cases without sibship of Fujian Han people was enrolled from July 2010 to September 2011, in which 68 healthy subjects as controls. One hundred and eighty-four patients with ACS were divided into three groups, 60 had one-vessel, 68 had two-vessel, and 54 had three-vessel lesion, 2 had not been found stenosis. DNA was extracted from venous blood, different groups of OPG gene polymorphism was identified by Sanger Method. OPG 209G/A, 245T/G and 1181G/C genotype frequencies and allelic frequencies were analyzed between ACS subjects and normal subjects and among the three groups of ACS, and the relationship between gene polymorphisms and ACS severity.</p><p><strong>Results: </strong>No significant differences were observed in the genotype and allelic frequencies of OPG gene 209G/A, 245T/G between the ACS and control groups. Significant differences were observed in the genotype and allelic frequencies of OPG gene 1181G/C between the ACS and control groups [1181G/C GG: 92 (50.0%) vs. 43 (63.2%), CC: 26 (14.1%) vs. 0, GC: 66 (35.9%) vs. 25 (36.8%), χ(2)=11.240, P=0.004; allelic gene G: 250 (67.9%) vs. 111 (81.6%), allelic gene C: 118 (32.1%) vs. 25 (18.4%), χ(2)=9.148, P=0.002]. No significant differences were observed in the genotype and allelic frequencies of OPG gene 209G/A, 245T/G and 1181G/C between those groups.</p><p><strong>Conclusions: </strong>There were no significant difference in frequencies OPG genotype and allele in polymorphisms 209G/A, 245T/C between patients with ACS and control group in Han population of Fujian province. There were difference in frequencies OPG genotype and allele in polymorphisms 1181G/C between patients with ACS and control group, but it was not differently distributed among patients with single-, double-, or triple-vessel lesion.</p>","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"24 12","pages":"730-3"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31063183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A perception of cardio-circulatory dysfunction in critical conditions].","authors":"Hong Shen","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"24 12","pages":"707-8"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31064328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The research of establishing discriminant function for patients with angina pectoris by stepwise analysis based on serum inflammatory factors].","authors":"Zhi-bin Chen,&nbsp;Yan-bing Liang,&nbsp;Hao Tang,&nbsp;Zhong-hua Wang,&nbsp;Li-jin Zeng,&nbsp;Jing-guo Wu,&nbsp;Zhen-yu Li,&nbsp;Zhong-fu Ma","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To improve cost-efficiency, discriminant functions in stepwise method was founded for the differential diagnosis of angina pectoris by detecting the serum level of high-sensitivity C-reactive protein (hs-CRP), macrophage migration inhibitory factor (MIF), interleukin-4 (IL-4) and interleukin-10 (IL-10) in patients with stable angina pectoris (SAP) and unstable angina pectoris (UAP).</p><p><strong>Methods: </strong>Thirty-nine SAP patients and 47 UAP patients were enrolled into the study, while 39 healthy volunteers were enrolled into the controlled group forming the entire set of training samples. The serum levels of hs-CRP, MIF, IL-4 and IL-10 were measured by enzyme linked immunosorbent assay (ELISA). Data was analyzed by software to define discriminant functions in the ways of \"entering\" and \"stepwise\". Both functions were evaluated by the results of validation.</p><p><strong>Results: </strong>By the way of \"enter independent together\", the following discriminant functions were defined based on the data of training samples' age, hs-CRP, MIF, IL-4, IL-10: healthy control group =-129.858 + 2.869×age -2.451×hs-CRP + 1.393×MIF + 6.001×IL-4 + 4.848×IL-10; SAP group=-161.037 + 2.896×age-2.022×hs-CRP + 1.662×MIF + 6.703×IL-4 + 6.287×IL-10; UAP group=-199.087 + 2.468×age-1.440×hs-CRP + 3.404×MIF-13.875×IL-4 + 7.752×IL-10. Retrospective validation showed 4.8% of total miss-grouping, while cross-validation showed 5.6% of total miss-grouping. By the way of \"stepwise\", the above data was screened by software and training samples' age, MIF and IL-10 were suggested to define the following functions: healthy control group = - 125.218 + 2.659 × age + 0.599×MIF + 5.040 × IL-10; SAP group=-157.864 + 2.721×age + 1.008×MIF + 6.468×IL-10; UAP group=- 197.327 + 2.360×age + 2.932×MIF + 7.640×IL-10. Both retrospective and cross validation showed 6.4% of total miss-grouping. Both sets of discriminant functions had the same efficiency (100%) for differential diagnosis of SAP and UAP.</p><p><strong>Conclusion: </strong>The discriminant functions based on samples' age, MIF and IL-10, which were screened and suggested by stepwise method, may contribute to the differential diagnosis of atypical SAP and UAP, and therefore demonstrate better cost-efficiency.</p>","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"24 12","pages":"713-6"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31064330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Evaluation of circulating microRNA-92a for endothelial damage induced by percutaneous coronary intervention].","authors":"Hong Wang, Lin Wang, Ling Liu, Ying-zhong Lin, You-qiong Li, Hong-mei Lu, G. Gong","doi":"10.3760/CMA.J.ISSN.1003-0603.2012.12.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1003-0603.2012.12.006","url":null,"abstract":"OBJECTIVE\u0000To explore the role of microRNA-92a (miR-92a) in evaluating endothelium damage induced by percutaneous coronary intervention (PCI).\u0000\u0000\u0000METHODS\u0000A case control study was prospectively conducted. Fifty-eight patients with ST-segment elevation acute myocardial infarction (STEAMI) received PCI were enrolled. MiR-92a expression in circulation was determined on the next day after PCI (reverse transcription-polymerase chain reaction). The correlation between miR-92a expression in circulation and PCI influence factors, such as inflation pressure, duration of balloon inflation and length of culprit atheromatous plaque were explored.\u0000\u0000\u0000RESULTS\u0000MiR-92a was lower in inflation pressure 11-19 atm (1 atm=101.325 kPa) group [n=43, mean -0.36, 95% confidence interval (95%CI) -0.60 to -0.12] than inflation pressure ≤10 atm group (n=11, mean 1.16, 95%CI 0.80 to 1.52, P<0.01) and ≥ 20 atm group (n=4, mean 0.26, 95%CI 0.26 to 0.26, P=0.1); and also lower in duration of balloon inflation 6-7 seconds group (n=24, mean -0.42, 95%CI -0.83 to -0.01) than in duration of balloon inflation ≤ 5 seconds group (n=9, mean 0.63, 95%CI 0.49 to 0.78, P=0.03) and ≥ 8 seconds group (n=25, mean 0.45, 95%CI 0.10 to 0.80, P<0.001); lower in implanted stent length ≤30 mm (n=31, mean -0.48, 95%CI -0.80 to -0.16) than those >30 mm (n=27, mean 0.16, 95%CI 0.01 to 0.32, P<0.01). A significantly negative correlation was found between inflation pressure and duration of balloon inflation. (r=-0.48, P<0.001).\u0000\u0000\u0000CONCLUSIONS\u0000There is a relationship between circulating miR-92a, inflation pressure and duration of balloon inflation. Circulating miR-92a could be used to evaluate the endothelium injury induced by PCI, and be used as a new target of prevention and treatment of endothelial dysfunction following revascularization.","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"43 1","pages":"721-4"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79407354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Urine cardiac specific microRNA-1 level in patients with ST segment elevation acute myocardial infarction].","authors":"Xiao-xia Duan,&nbsp;Xian Zhou,&nbsp;Xiao-bin Wang,&nbsp;Zhong-cai Fan,&nbsp;Chun-xiang Zhang,&nbsp;Zhuang Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To observe the change of urine level of cardiac specific microRNA-1 (miR-1) in patients with ST segment elevation acute myocardial infarction (STEAMI) and investigate its potential applications.</p><p><strong>Methods: </strong>Urine samples were collected from 20 STEAMI patients within 12 hours after STEAMI and from 20 healthy volunteers as control. Urine miR-1 concentrations were measured with real-time quantity reverse transcription-polymerase chain reaction (qRT-PCR), at the same time serum cardiac troponin I (cTnI) and MB isoenzyme of creatine kinase (CK-MB) concentrations were measured.</p><p><strong>Results: </strong>Serum level of cTnI, CK-MB and urine level of miR-1 in STEAMI patients were obviously higher than those in healthy control group [cTnI in blood: 19.27±7.53 μg/L vs. 0.02±0.01 μg/L, CK-MB in blood: 93.82±12.30 μg/L vs. 0.86±0.63 μg/L, miR-1 in urine (Ct value): 45.50±4.21 vs. 52.63±4.41, P<0.05 or P<0.01]. The urine miR-1 level in patients with STEAMI had a strong correlation with serum CK-MB or cTnI when CK-MB < 300 μg/L and cTnI <50 μg/L (Ct value of urine miR-1 with blood CK-MB: r=-0.81, P<0.01; Ct value of urine miR-1 with blood cTnI: r=-0.63, P<0.05).</p><p><strong>Conclusions: </strong>The results suggest that urine miR-1 could be a novel sensitive biomarker the early diagnosis of SETAMI.</p>","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"24 12","pages":"709-12"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31064329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Changes in cardiac specific microRNA-208a level in peripheral blood in ST segment elevation acute myocardial infarction patients].","authors":"Yi Yao, Xian Zhou, Xiao-bin Wang, Xiaoxia Duan, Chun-xiang Zhang, Xiao-ling Shi, Zhuang Chen, Zhong-cai Fan","doi":"10.3760/CMA.J.ISSN.1003-0603.2012.12.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1003-0603.2012.12.005","url":null,"abstract":"OBJECTIVE\u0000To observe serum cardiac specific microRNA-208a (miR-208a) levels in ST segment elevation acute myocardial infarction (STEAMI) patients, and to explore the role of serum miR-208a levels in the diagnosis of STEAMI.\u0000\u0000\u0000METHODS\u0000The serum miR-208a concentrations were assessed within 12 hours after STEAMI, while 30 healthy individuals as control. Serum miR-208a concentrations were measured with real-time quantity reverse transcription-polymerase chain reaction (qRT-PCR), and serum cardiac troponin I (cTnI) or MB isoenzyme of creatine kinase (CK-MB) concentrations were measured with enzyme linked immunosorbent assay (ELISA).\u0000\u0000\u0000RESULTS\u0000The contents of serum cTnI or CK-MB in STEAMI patients were significantly higher than those in healthy individuals (cTnI: 17.72±8.43 μg/L vs. 0.05±0.01 μg/L, CK-MB: 250.83±177.26 μg/L vs. 71.20±20.50 μg/L, both P<0.01). Serum miR-208a concentrations were detected in all individuals with STEAMI within 60 PCR cycle (0-6 hours: 44.95±4.77, 6-12 hours: 43.98±4.68), but were beyond detection for all individuals in the healthy control group. The serum miR-208a relative levels in STEAMI patients were at least more than 215 fold than that in healthy persons, compared with qRT-PCR (Ct=60) of miR-208a in healthy control persons (P<0.01).\u0000\u0000\u0000CONCLUSION\u0000Serum miR-208a may be a new biomarker the early diagnosis of STEAMI patients.","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"70 1","pages":"717-20"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79303355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical characteristics of coronary artery disease patients with reduced left ventricular ejection fraction and their prognostic analysis].","authors":"Quan Li, Yue-chun Gao, Jiqiang He, T. Jiang, X. Ren, Fang Chen","doi":"10.3760/CMA.J.ISSN.1003-0603.2012.12.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1003-0603.2012.12.010","url":null,"abstract":"OBJECTIVE\u0000To explore the relationship between reduced left ventricular ejection fraction (LVEF) and characteristics of coronary artery disease (CAD) and investigate the association between reduced LVEF and cardiovascular prognosis.\u0000\u0000\u0000METHODS\u0000A total of 677 hospitalized patients with angiographic CAD were enrolled. All patients' clinical data were recorded. LVEF were measured, high sensitive C-reactive protein (hs-CRP), white blood cell (WBC) and classic cardiovascular risk factors were recorded after admission. All patients were followed up from admission. The primary end point was combination occurrence of major adverse cardiovascular and cerebral events (MACCE), including death, targeted vascular revascularization, non-fatal myocardial infarction and rehospitalization due to unstable angina or heart failure, transient ischemic attack or stroke.\u0000\u0000\u0000RESULTS\u0000All patients were tracked for (15±12) months, and patients were divided into normal LVEF group (LVEF≥0.50, n=585) and reduced LVEF group (LVEF<0.50, n=92) according to LVEF level. Compared with normal LVEF group, reduced LVEF group had more severe coronary stenosis (Gensini score: 62.85±41.45 vs. 47.68±33.26, P<0.05), a higher level of WBC and hs-CRP (WBC: 7.60±2.71 ×10(9)/L vs. 7.09±2.13 ×10(9)/L, hs-CRP: 5.68±3.97 mg/L vs. 3.97±3.75 mg/L, both P<0.05). A total of 146 MACCE occurred during follow-up periods. Compared with no-MACCE group, LVEF levels were significantly lower in MACCE group (0.576±0.113 vs. 0.603±0.101) and there were a higher level of hs-CRP and Gensini score in MACCE group (hs-CRP: 5.26±3.99 mg/L vs. 3.91±3.72 mg/L, Gensini score: 53.72±35.50 vs. 48.63±34.59, all P<0.05). Moreover, both of univariate and multivariate Cox regression analysis indicated LVEF be an independent predictor of MACCE in patients with CAD [univariate: relative risk (RR)=0.974, 95% confidence interval (95%CI) 0.960 to 0.988, P=0.000; multivariate: RR=0.979, 95%CI 0.961 to 0.998, P=0.033]. Kaplan-Meier analysis suggested that patients with reduced LVEF had an increased MACCE occurrence (χ(2)=14.56, P<0.05).\u0000\u0000\u0000CONCLUSION\u0000LVEF level may be associated with coronary artery severity, and could be independently predict the prognosis of CAD.","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"32 1","pages":"734-8"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81061897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Urine cardiac specific microRNA-1 level in patients with ST segment elevation acute myocardial infarction].","authors":"Xiaoxia Duan, Xian Zhou, Xiao-bin Wang, Zhong-cai Fan, Chun-xiang Zhang, Zhuang Chen","doi":"10.3760/CMA.J.ISSN.1003-0603.2012.12.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1003-0603.2012.12.003","url":null,"abstract":"OBJECTIVE\u0000To observe the change of urine level of cardiac specific microRNA-1 (miR-1) in patients with ST segment elevation acute myocardial infarction (STEAMI) and investigate its potential applications.\u0000\u0000\u0000METHODS\u0000Urine samples were collected from 20 STEAMI patients within 12 hours after STEAMI and from 20 healthy volunteers as control. Urine miR-1 concentrations were measured with real-time quantity reverse transcription-polymerase chain reaction (qRT-PCR), at the same time serum cardiac troponin I (cTnI) and MB isoenzyme of creatine kinase (CK-MB) concentrations were measured.\u0000\u0000\u0000RESULTS\u0000Serum level of cTnI, CK-MB and urine level of miR-1 in STEAMI patients were obviously higher than those in healthy control group [cTnI in blood: 19.27±7.53 μg/L vs. 0.02±0.01 μg/L, CK-MB in blood: 93.82±12.30 μg/L vs. 0.86±0.63 μg/L, miR-1 in urine (Ct value): 45.50±4.21 vs. 52.63±4.41, P<0.05 or P<0.01]. The urine miR-1 level in patients with STEAMI had a strong correlation with serum CK-MB or cTnI when CK-MB < 300 μg/L and cTnI <50 μg/L (Ct value of urine miR-1 with blood CK-MB: r=-0.81, P<0.01; Ct value of urine miR-1 with blood cTnI: r=-0.63, P<0.05).\u0000\u0000\u0000CONCLUSIONS\u0000The results suggest that urine miR-1 could be a novel sensitive biomarker the early diagnosis of SETAMI.","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"1 1","pages":"709-12"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73083795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Protective effect of melatonin on myocardial injury in severely- burned rats].","authors":"Xiao-hua Han,&nbsp;Luo Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the protective effect of melatonin (MLT) on myocardial injury in severely-burned rats and its mechanism.</p><p><strong>Methods: </strong>A total of 30 Sprague-Dawley (SD) rats were randomly assigned to three groups: sham group, burn group and MLT group, each n=10. The dorsal skin of animal was immersed into boiling water for 15 seconds to induce 30% total body surface area (TBSA) full-thickness burn, or immersed into 37 centigrade water for sham operation. Immediately after burn, the animals in burn group and MLT group were given intraperitoneally vehicle (1% alcohol in normal saline) or MLT (10 mg/kg) respectively. Six hours postburn, the blood from tail vessel was collected for serum preparation. After sacrificed, the myocardial tissues of rats were collected for the determination of malondialdehyde (MDA) and reduced glutathione (GSH) as well as glutathione peroxidase (GSH-Px) and myeloperoxidase (MPO) activities. Serum levels of creatine kinase (CK) and lactate dehydrogenase (LDH) were also estimated.</p><p><strong>Results: </strong>Compared with the sham control, burn injury increased MDA by 66.7% (1.55±0.17 nmol/mg vs. 0.93±0.05 nmol/mg) and decreased GSH by 27.8% (13.58±0.33 nmol/mg vs. 18.82±0.55 nmol/mg, both P<0.01) in myocardial tissues, GSH-Px activity was also slightly inhibited (74.04±3.42 nmol×min(-1)×mg(-1) vs. 93.79±3.76 nmol×min(-1)×mg(-1), P<0.05), but MPO level was found to increase to 2.8 folds (9.43±1.15 U/g vs. 3.41±0.27 U/g, P<0.01). These changes indicated the occurrence of oxidative stress in myocardial tissues after severe burn. MLT treatment relieved most of the abnormality with significant statistical significances (MDA: 0.89±0.08 nmol/mg vs. 1.55±0.17 nmol/mg, GSH: 17.23±0.54 nmol/mg vs. 13.58±0.33 nmol/mg, MPO: 6.91±0.51 U/g vs. 9.43±1.15 U/g, P<0.05 or P<0.01). In addition, the serum levels of CK and LDH in burn group increased to 37.8 folds and 7.4 folds respectively (both P<0.01). MLT treatment reduced CK by 32.9% and reduced LDH by 21.2% (P<0.05 and P<0.01).</p><p><strong>Conclusion: </strong>MLT treatment exerts the protective effect on myocardial injury in severely-burned rats, which is attributed predominantly to its inhibition on burn-induced oxidative stress injury.</p>","PeriodicalId":23992,"journal":{"name":"Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue","volume":"24 12","pages":"747-9"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31063187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}