Tunde Olowolafe, O. Popoola, O. Fagbenro, Olaronke Olamide Olawusi-Peters
{"title":"Chronic Toxicity of True Indigo (Indigofera tinctoria) Leaf Powder on Antioxidant Status and Haematological Profile of Juveniles African Cat Fish (Clarias gariepinus)","authors":"Tunde Olowolafe, O. Popoola, O. Fagbenro, Olaronke Olamide Olawusi-Peters","doi":"10.4194/trjfas21741","DOIUrl":"https://doi.org/10.4194/trjfas21741","url":null,"abstract":"The study was conducted to test the toxicity of Indigofera tinctoria on the antioxidative status and heamatology of Clarias gariepinus. Matured leaves of I. tinctoria were collected, dried, and pulverized. Graded levels of the I. tinctoria leaf powder were weighed and dissolved in 250 ml of water. Data obtained was subjected to one-way to analyse the significant differences between groups and significance level of P<0.05 was used for all tests. Hematological indices indicated a significant (P<0.05) decreasing trend in the red blood cell, haemoglobin and the packed cell volume with increasing concentrations of I. tinctoria while the white blood cell counts increased with increasing concentrations of the toxicant. Stress levels inflicted by the test plant were assessed by estimating the effects on the oxidative stress biomarkers in the kidney, liver, muscle and gill of C. gariepinus. After the 21 days exposure, activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and catalase (CAT) were investigated and all increased when compared to control. The findings revealed that the disposal of leaves, and effluent from I. tinctoria could threaten the life and existence of the aquatic organism.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48464463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Upwelling Index Calculations in the Aegean Sea","authors":"Emre Tükenmez, H. Altiok","doi":"10.4194/trjfas21621","DOIUrl":"https://doi.org/10.4194/trjfas21621","url":null,"abstract":"Different upwelling index calculations used for oceans were tested by applying to the Aegean Sea and compared with each other. The sea surface temperature (SST) and wind data of ECMWF Reanalysis are used for upwelling index calculations. The decrease in SST is a result of upwelling; however, the parameter that lies behind the phenomenon is the wind stress on the sea surface. The results show that SST and wind-driven upwelling indexes could be roughly used to estimate upwelling phenomena in the Aegean Sea, but it is insufficient for the precise determination of upwelling due to the Aegean Sea`s unique dynamics. To reach a final formula, the `new upwelling index` shall be improved for Aegean Sea in which current data are considered besides SST & wind data.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42787002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Roberto Vázquez-Euan, Estefanía Garibay-Valdez, M. Martínez‐Porchas, L. R. Martínez-Córdova, L. F. Enríquez-Ocaña, R. Vílchez-Vargas, K. Calderón
{"title":"Effect of different probiotic diets on microbial gut characterization and gene expression of Litopenaeus vannamei cultivated in BFT system","authors":"Roberto Vázquez-Euan, Estefanía Garibay-Valdez, M. Martínez‐Porchas, L. R. Martínez-Córdova, L. F. Enríquez-Ocaña, R. Vílchez-Vargas, K. Calderón","doi":"10.4194/trjfas21358","DOIUrl":"https://doi.org/10.4194/trjfas21358","url":null,"abstract":"Microbial communities, mainly those with probiotic characteristics, positively affect the culture of diverse organisms, including shrimp. In this study, three diets containing different types of probiotics were evaluated on the composition and diversity of the microbial community in the water column and gut of Litopenaeus vannamei, as well as on the expression of genes related to the immune system and resistance of the organisms after being challenged with WSSV and Vibrio parahaemolyticus. Results showed that probiotics affected the microbial community structure in water and shrimp. The expression of beta-glucan binding protein and prophenoloxidase increased in the treatments with probiotics compared to the control before and after the challenge. Overall the survival of shrimp was higher in all the treatments than in control. Results suggest that using probiotics in BFT systems could provide an extra benefit for these kinds of systems.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44495914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rabia Anwar Rao, A. Qurashi, I. Liaqat, M. Gohar, U. Zafar, Muhammad Amjad Khan, Saraj Bahadur
{"title":"Utilization of Fish Scales for Microbe-mediated Gelatin Extraction: A New Biotechnology and Microbiology Avenue","authors":"Rabia Anwar Rao, A. Qurashi, I. Liaqat, M. Gohar, U. Zafar, Muhammad Amjad Khan, Saraj Bahadur","doi":"10.4194/trjfas21768","DOIUrl":"https://doi.org/10.4194/trjfas21768","url":null,"abstract":"Waste materials are of major concern around the globe. This study aimed to utilize the scales of fish (Labeo rohita) in microbe-mediated gelatin extraction. Three bacterial isolates were isolated from the fish scales and one bacterial isolate FSW3 showing maximum gelatin yield (61.4 %) was subjected to further analysis. The bacterial culture FSW3 (identified as Staphylococcus spp.) with enhanced production of extracted gelatin was set for physiochemical testing, FTIR analysis, UV-Vis spectroscopy, and stereomicroscopic analysis. The extracted gelatin was successfully utilized in various applications including plant growth promotion under salt stress, anti-adhesion films, firmness of yogurt, biodegradable plastic, and anti-microbial food coatings. Results showed that 0.01 mM gelatin promoted plant growth promotion under salt stress. FSW3 extracted gelatin decreased the 2-fold biofilm-forming ability of E. coli, when exposed to the gelatin-based anti-adhesion films coated on a glass slide and polystyrene sheet. The gelatin showed promising findings as anti-microbial food coatings for tomatoes preservation after 21 days and helpful in the firmness of yogurt. Another advantage of gelatin was casting it into biodegradable plastic. The current study provides a promising approach for fish scales microbe-mediated gelatin extraction and uses it to explore new avenues in biotechnology and microbiology.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45299248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. A. Shaaban, S. Tawfik, Wael El-Tarras, T. El-Sayed Ali
{"title":"Are The Water Quality of the Agricultural Drainage and Nile River Suitable for Tilapia Culture? A Case Study from Kafr El-Shaikh's Fish Farms, Egypt","authors":"N. A. Shaaban, S. Tawfik, Wael El-Tarras, T. El-Sayed Ali","doi":"10.4194/trjfas21395","DOIUrl":"https://doi.org/10.4194/trjfas21395","url":null,"abstract":"Due to freshwater scarcity, the use of drainage water for aquaculture is a severe challenge in Egypt (one of the cultured tilapia producers worldwide). The present study was conducted, in the highest fish producer governorate (Kafr El-Sheikh). The study aims to assess the water quality of different fish farms fed with different water: agricultural drainage (ADW) and Nile River (Ri-N) water, and examine their suitability for fish culturing. Environmental parameters, nutrients, and metals in the water of fishponds and their sources, were measured. Results revealed that fish farms' waters were brackish (salinity <12 ppt), and slightly alkaline (pH of 8.5). The distribution of DO and BOD between farms was significantly different (p-value<0.05). Metals varied from high Fe levels to below detection limits (Pb and Cd). Feeding water sources showed lower metal content. However, farm fed by Ri-N was enriched by nutrients and attend low metals contents relative to farms fed by ADW, which was verified by the significant statistical differences. Most studied parameters were lower than the acceptable guidelines for fisheries and aquatic life recommended by national and international standards. Overall, the water qualities of fish farms were in good condition and were suitable for tilapia culture.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42022100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Koffi Bienvenu N’Goran, K. J. Konan, Kouassi Yves Narcisse Kouamé, Annigbé Justin Eyi, K. Kouakou
{"title":"Reproductive Biology of the Hybrid Coptodon guineensis x Coptodon zillii within Two Marginal Lagoons Invaded by Macrophytes in South Eastern of Côte d`Ivoire","authors":"Koffi Bienvenu N’Goran, K. J. Konan, Kouassi Yves Narcisse Kouamé, Annigbé Justin Eyi, K. Kouakou","doi":"10.4194/trjfas21680","DOIUrl":"https://doi.org/10.4194/trjfas21680","url":null,"abstract":"Hybrids of Coptodon guineensis x Coptodon zillii are omnivorous species with a phytophagous tendency found in most of water bodies of Côte d`Ivoire. This study aims to better understand its reproductive biology and possible influence of macrophytes on the breeding season. A total of 608 fish were caught monthly from September 2017 to August 2019. The overall sex-ratio showed significant predominance of females in Ono (1:1.37; χ² = 7.10; P<0.05) and Kodjoboue (1:1.84; χ² = 28.62; P<0.05) lagoons. The sexual maturity recorded in Ono lagoon for males (148.2 mm, SL) and females (145.3 mm) was slightly higher than that of males (144.4 mm, SL) and females (141.8 mm) in Kodjoboue lagoon. March and August–October (Ono lagoon) and February and July–September (Kodjoboue lagoon) were the most intensive spawning months. The absolute fecundity was higher in Ono lagoon (1472–6116 oocytes) than in Kodjoboue lagoon (1676–4210 oocytes), indicating a better food supply due to the high abundance of macrophytes in Ono lagoon. Oocyte size frequency distributions show oocytes of different sizes, indicating more than one spawning within a reproduction season. These findings might facilitate future studies establishing its potential production and its exploitation and management.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48280821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"De Novo Assembly and Annotation of Microalga Tetradesmus obliquus Transcriptome","authors":"Omer Can Unuvar, E. Unlu","doi":"10.4194/trjfas21341","DOIUrl":"https://doi.org/10.4194/trjfas21341","url":null,"abstract":"Tetradesmus obliquus is a unicellular green microalga and considered as a potential source for biotechnological production of pigments such as lutein. No genome-related data is available for T. obliquus that would increase the ability to develop new approaches in biotechnological applications. We present the first transcriptome data for T. obliquus. The aim of this study is to provide a comprehensive transcriptome annotation and identification of conserved genes involved in lutein pigment biosynthesis in Tetradesmus obliquus cells by analyzing pooled RNA-Seq data. Next-Generation Sequencing was applied for the pooled cDNAs library prepared by combining the cell cultures collected from samples exposed to dark and high light intensity conditions. Transcripts were assembled by the de novo assembly approach. Trinotate software was used for functional annotation of assembled transcripts. We also carried out BLAST analysis comparing the transcriptome data against known lutein biosynthesis genes. The 49.15% of the assembled sequences were functionally annotated, providing a total of 21490 unigenes. Our data also revealed the transcript sequences for ten conserved genes required for lutein biosynthesis. The data produced in this study can be used for molecular approaches in biotechnological applications related to T. obliquus, such as increasing the yield of pigment production.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46483944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of Pseudoalteromonas sp. from Aquaculture Environment and Optimization of Fermentation Culture Parameters by RSM-Based Modeling","authors":"Ecren Uzun Yaylacı","doi":"10.4194/trjfas21726","DOIUrl":"https://doi.org/10.4194/trjfas21726","url":null,"abstract":"The aim of the study was to identify Pseudoalteromonas strain OS-9 and evaluate its anti-Vibrio potential. Based on the morphological, biochemical, and 16S rRNA sequence analysis, the strain was identified as Pseudoalteromonas haloplanktis. Experiments designed with Box Behnken showed that cell density and cell-free supernatant activity were simultaneously maximum at 30°C, pH 7, and 90 h. The coefficient values obtained from the response surface methodology regression equations determined that pH was more effective on responses than other physical variables. The cell-free supernatant of P. haloplanktis OS-9 extracted under optimized conditions was only effective on Gram-negative fish pathogenic bacteria and especially on Vibrio. Among Vibrio spp., the strongest inhibitory effect was observed against V. rotiferianus. At the maximum cell density (10.48 OD), the strain OS-9 showed the maximum inhibitory effect against V. rotiferianus (26.42 mm zone diameter). In co-culture assay, the growth of V. rotiferianus was inhibited after 96 h with an initial level of 1.0 × 106 CFU ml-1 by the strain OS-9. These results indicated that P. haloplanktis strain OS-9 could be considered as a potential Vibrio bio-controlling agent.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45071297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samuel Ofori‐Mensah, M. Yıldız, Vahap Eldem, Cigdem Urku, Çağlayan Kaplan
{"title":"Effect of dietary inclusion of camelina or chia oil on fatty acid digestibility, histology, blood biochemistry and molecular biomarkers in juvenile gilthead sea bream (Sparus aurata, L.)","authors":"Samuel Ofori‐Mensah, M. Yıldız, Vahap Eldem, Cigdem Urku, Çağlayan Kaplan","doi":"10.4194/trjfas20857","DOIUrl":"https://doi.org/10.4194/trjfas20857","url":null,"abstract":"The present study evaluated fatty acid digestibility, histology, blood biochemistry and gene expression in the gut of juvenile gilthead sea bream, Sparus aurata (initial mean weight, 60.4 ± 4.9 g) fed with iso-proteic (48%) and iso-lipidic (20.7%) diets in which fish oil was partially (60%) or totally (100%) replaced with either camelina or chia oil. Each of the experimental diets was fed to triplicate groups of fish stocked at 10 fish/tank in 150 L tanks for 90 days. Although inclusion of camelina or chia oil altered dietary fatty acid (FA) profile, major differences in digestibility in the individual FAs were not observed. Total replacement of fish oil with camelina or chia oil did not have any effect on blood glucose levels, whereas cholesterol and triglyceride levels were reduced. Results of messenger ribonucleic acid (mRNA) transcription showed elevated levels in the expression of the elongases and desaturases in gilthead seabream fed with diets devoid of fish oil. Fish fed with camelina or chia oil based diets had increased expression in lipolytic and lipogenic genes, resulting in accumulation of lipid droplets in the gut of fish. Histological examination revealed no pathological disorders in the guts of the fish.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48956234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Visual LAMP Method for Detection of White Spot Syndrome Virus in Shrimp and Crayfish","authors":"Chao Ma, Z. Tian, Lu Bai, Lili Yang, Jijuan Cao","doi":"10.4194/trjfas21519","DOIUrl":"https://doi.org/10.4194/trjfas21519","url":null,"abstract":"White spot syndrome virus (WSSV) has caused large economic losses to the aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. Therefore, rapid detection of white spot syndrome virus (WSSV) in shrimp is crucial. This study was conducted to establish a rapid visual detection method for WSSV in shrimp and on the basis of loop-mediated isothermal amplification (LAMP). The color change of the amplification products was observed using a fluorescence amplification curve (with the SYTO-9 fluorescent dye) and LAMP visualization (with calcein), and the method`s specificity, sensitivity, and reproducibility were analyzed. It showed that this method can specifically detect WSSV, with a detection sensitivity of 1 fg/μL and has good reproducibility and reliability. This method was used to analyze cultured shrimp and crayfish samples; among 329 suspected clinical samples, the positive diagnostic rate of WSSV was 27.96%. The infection activity of WSSV was the highest at temperatures of 20–26°C. The LAMP-based method for rapid visual detection of WSSV does not need complex experimental instruments, and an equipment with a stable heat source is sufficient for the reaction. It is easy to use and can be used at shrimp breeding sites.","PeriodicalId":23978,"journal":{"name":"Turkish Journal of Fisheries and Aquatic Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44108720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}