Transactions of the American Microscopical Society最新文献_第4页

Occurrence of ferritin crystals in thoracic and abdominal cells of the adult parasitic wasp Pediobius foveolatus (Hymenoptera: Eulophidae) 黄斑小蜂成虫胸腹细胞中铁蛋白晶体的出现(膜翅目:蜂科)

Transactions of the American Microscopical Society Pub Date : 1994-01-01 DOI: 10.2307/3226579

G. Chapman, M. Hooker

引用次数: 1

Alloglossidium greeri n. sp. (Digenea: Macroderoididae) from the Cajun dwarf crayfish, Cambarellus schufeldti, in Louisiana, U.S.A. 美国路易斯安那州卡津小龙虾(Cambarellus schufeldti)的绿小龙虾(Alloglossidium greeri n. sp.

Transactions of the American Microscopical Society Pub Date : 1994-01-01 DOI: 10.2307/3226583

W. F. Font

引用次数: 9

Morphology and Chorionic Fine Structure of the Egg of Neurocolpus nubilus (Hemiptera: Miridae) 黄斑神经蝗卵的形态和绒毛膜精细结构(半翅目:蝗科)

Transactions of the American Microscopical Society Pub Date : 1994-01-01 DOI: 10.2307/3226582

G. Baker, P. W. Ma

引用次数: 3

Fine structure of the gastrodermis of two species of Gyrodactylus (Monogenoidea: Polyonchoinea, Gyrodactylidae) 两种旋齿蝇腹真皮的精细结构(单总科:蓼目，旋齿蝇科)

Transactions of the American Microscopical Society Pub Date : 1994-01-01 DOI: 10.2307/3226578

D. C. Kritsky, D. Bourguet, R. Spall

{"title":"Fine structure of the gastrodermis of two species of Gyrodactylus (Monogenoidea: Polyonchoinea, Gyrodactylidae)","authors":"D. C. Kritsky, D. Bourguet, R. Spall","doi":"10.2307/3226578","DOIUrl":"https://doi.org/10.2307/3226578","url":null,"abstract":"Transmission electron microscopy was used to determine the organization and fine structure of the gastrodermis of two species of Gyrodactylus. The gastrodermis consists of a syncytial epithelium characterized by large widely spaced nuclei, two types of vesicular structures, mitochondria with tubular cristae, Golgi complexes, and scattered rough endoplasmic reticulum; the luminal surface possesses few widely spaced, branched lamellae projecting into the intestinal lumen. Organization of the gastrodermis in the Monogenoidea is discussed; the syncytial gastrodermis is considered to be a possible synapomorphic feature of the Gyrodactylidea. Two general patterns of nutrition occur within the Monogenoidea (see Halton & Jennings, 1965). In the subclass Polyonchoinea, member species usually feed on epidermal tissues and secretions of the host, and species of the subclasses Polystomatoinea and Oligonchoinea are generally sanguinivorous. These nutritional preferences appear to be related to gastrodermal structure and organization. In the Polystomatoinea and Oligonchoinea, the gut wall is comprised of two cell types, one of which is involved in digestion of blood (see Rohde, 1980, for review). In polyonchoinean species investigated to date, the gastrodermis comprises a monolayer of cells consisting of a single cell type (Fournier, 1978; Halton & Stranock, 1976; Junchis, 1988). Apparently, the latter organization of the gastrodermis is symplesiomorphic for the Monogenoidea in that a similar state also occurs among rhabdocoel turbellarians (Holt & Mettrick, 1975) as well as among some digeneans and aspidobothreans (Dike, 1967; Hathaway, 1972; Rohde, 1971). The purpose of this paper is to report an apomorphic state of the gastrodermis within the derived polyonchoinean family Gyrodactylidae. MATERIALS AND METHODS Specimens of Gyrodactylus eucaliae Ikezaki & Hoffman, 1957 and Gyrodactylus sp. were collected from the skins of Culaea inconstans (Kirtland) in Illinois and Rhinichthys osculus (Girard) in Idaho, respectively. Preparatory procedures of G. eucaliae for electron microscopy were presented by Kritsky & Kruidenier (1976). Specimens of Gyrodactylus sp. were fixed at 0?C for 4 h in a solution of equal parts of 1.5% paraformaldehyde and 1.5% glutaraldehyde buffered at 7.3 pH with 0.1 M sodium cacodylate. After postfixation in buffered 1% OsO4 for 20 min, the worms were washed in distilled water and dehydrated The authors are grateful to Mr. Jim Smothers, Department of Biological Sciences, Idaho State University, for assistance in the electron-microscope laboratory. TRANS. AM. MICROSC. Soc., 113(1): 43-51. 1994. ? Copyright, 1994, by the American Microscopical Society, Inc. This content downloaded from 207.46.13.176 on Mon, 20 Jun 2016 07:33:45 UTC All use subject to http://about.jstor.org/terms TRANS. AM. MICROSC. SOC.","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"52 1","pages":"43-51"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83550531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

An ultrastructural study of Didymosphenia geminata (Bacillariophyceae) 双叶藻(硅藻科)的超微结构研究

Transactions of the American Microscopical Society Pub Date : 1994-01-01 DOI: 10.2307/3226580

Mary C. Moffat

引用次数: 14

Two new species of Isospora (Apicomplexa: Eimeriidae) from ovenbirds (Passeriformes: Furnariidae) of South America 标题南美烧鸟(雀形目:烧鸟科)等孢子虫属二新种

Transactions of the American Microscopical Society Pub Date : 1994-01-01 DOI: 10.2307/3226584

T. E. Mcquistion, A. Capparella

引用次数: 4

Mounting pollen on a thermoplastic adhesive for scanning electron microscopy 将花粉装在扫描电子显微镜用的热塑性粘合剂上

Transactions of the American Microscopical Society Pub Date : 1994-01-01 DOI: 10.2307/3226581

W. Chissoe, E. L. Vezey, J. Skvarla

{"title":"Mounting pollen on a thermoplastic adhesive for scanning electron microscopy","authors":"W. Chissoe, E. L. Vezey, J. Skvarla","doi":"10.2307/3226581","DOIUrl":"https://doi.org/10.2307/3226581","url":null,"abstract":"A heat-sensitive adhesive, Tempfix?, developed for scanning electron microscopy of powders and small particles, provides consistently high-quality secondary imaging with a variety of pollen grain morphologies and preparation protocols. Tempfix\" was used successfully under all combinations of these conditions: (1) pollen air-dried from ETOH or artificially dried; (2) pollen transferred to naked or sputter-coated Tempfix?; and (3) pollen transferred to solid (at room temperature) or tacky (40-43?C) Tempfix?. In contrast to other adhesives, Tempfix\": (1) does not react with the dehydration fluid ethanol; (2) permits control of depth of pollen penetration into the adhesive; (3) provides a high pollen retention rate with both air-dried and artificially dried preparations; and (4) has a smooth glassy background. A critical element in many scanning electron microscopic (SEM) studies of pollen is the adhesive used to anchor grains to the specimen mount. Surprisingly, palynological adhesives have received relatively little attention. Frequently, they are unreported or only briefly noted in the materials-and-methods sections of pollen investigations. Adhesives for pollen grains are similar to those for other particulates; they include double-stick tape, rubber cement, silver paste, poly-L-lysine, glues, and waxes (Murphy, 1982). All of these, to some extent, yield inconsistent or undesirable results: charging (the buildup of electrons caused by inadequate sample grounding); sunken grains with halos attributable to partial embedment in the adhesive; grains with obscured surfaces caused by interaction of dehydrating solution with the adhesive; wrinkled, grainy, and uneven backgrounds owing to the nature and composition of the adhesive; and excessive loss of pollen grains caused by inadequate bonding with the adhesive. Recently, a thermoplastic adhesive, Tempfix?, was recommended as a mounting medium for small particles and powders in SEM (Marchese-Ragona et al., 1992). Because Tempfix? offered potential advantages over the adhesives listed above, particularly the ability to control the depth of sample penetration, we tested this adhesive using a variety of pollen grain sculpture patterns and SEM preparation techniques. MATERIALS AND METHODS Dry pollen was collected from herbarium specimens (Table I) and treated as outlined in Fig. 1. First, pollen was acetolyzed (10 min of boiling in a 9:1 mixture of acetic anhydride and sulfuric acid; Erdtman, 1960). All samples then were dehydrated through graded ethanol (ETOH) solutions to 100% ETOH, and then either (1) air-dried directly onto specimen mounts (Fig. 1: method TRANS. AM. MICROSC. Soc., 113(1): 72-79. 1994. ? Copyright, 1994, by the American Microscopical Society, Inc. This content downloaded from 157.55.39.243 on Wed, 05 Oct 2016 04:52:18 UTC All use subject to http://about.jstor.org/terms VOL. 113, NO. 1, JANUARY 1994","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"124 1","pages":"72-79"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90582239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Observations on the morphology and taxonomy of a marine ciliate species of the genus Diophrys (Ciliophora: Euplotida) 一种海洋纤毛虫的形态与分类观察(纤毛虫目:整虫目)

Transactions of the American Microscopical Society Pub Date : 1994-01-01 DOI: 10.2307/3226576

G. FERNANDEZ-LEBORANS, A. Novillo

{"title":"Observations on the morphology and taxonomy of a marine ciliate species of the genus Diophrys (Ciliophora: Euplotida)","authors":"G. FERNANDEZ-LEBORANS, A. Novillo","doi":"10.2307/3226576","DOIUrl":"https://doi.org/10.2307/3226576","url":null,"abstract":"The morphological features of a species of the genus Diophrys are described with special reference to ciliature and infraciliature. These characteristics consist of: (1) the oral ciliature composed of an adoral zone of organelles and a paroral formation, and (2) the frontal, ventral, left marginal, transverse, and caudal cirri. A biometric analysis was carried out taking into account 31 morphological features; these included: (1) body size and nuclear apparatus components; (2) size of the oral ciliature structures; (3) size of the different cirri; (4) relative distances between the transverse cirri and the poles; and (5) the length of cilia beyond the infraciliature. Comparisons were made with other species of the genus, taking into account 11 structural variables. The present paper: (1) defines the morphological characteristics of populations of D. appendiculata by completing descriptions of certain infraciliature structures, supplementing data of earlier studies by way of biometric analysis, and providing, by means of a morphometric survey, an assemblage of statistically treated data that may be used to make taxonomic determinations with greater precision. The genus Diophrys embraces several species of ciliates that are common in many marine habitats, especially in epibenthic zones of the continental shelf. Although these species were described by various workers (Agamaliev, 1967, 1983; Borror, 1972; Dragesco, 1963; Hartwig, 1973; Kahl, 1935; Kattar, 1970), there is a paucity of morphological data for individual species; e.g., Diophrys appendiculata (see Curds & Wu, 1983). We studied individuals of D. appendiculata, comparing their morphological features with other species of Diophrys. This paper shows: (1) morphological characteristics of a population of D. appendiculata; (2) morphological data obtained not only by us, but supplemented other data from the literature; (3) a biometric analysis of infraciliature and other structural characteristics, thus supplying statistical data on D. appendiculata; and (4) comparisons among different species of Diophrys that may be of taxonomic utility. MATERIALS AND METHODS Ciliates were collected from Castro Urdiales Beach (Santander, Spain) during 1989-1990. They were extracted from interstitial sediments using the procedure described by Fernandez-Leborans (1990). Specimens were studied using brightfield and phase-contrast microscopy. Ciliature and infraciliature were stained by the silver carbonate technique (Fernandez-Leborans & Castro de Zaldumbide, 1986a). Measurements for 80 individuals were obtained for each feature using a calibrated ocular micrometer. I This work was supported by C.I.C.Y.T. grant NAT90-0059. TRANS. AM. MICROSC. SOC., 113(1): 22-33. 1994. ? Copyright, 1994, by the American Microscopical Society, Inc. This content downloaded from 157.55.39.35 on Fri, 02 Sep 2016 04:26:02 UTC All use subject to http://about.jstor.org/terms VOL. 113, NO. 1, JANUARY 1994 RESULTS General Morphology Specim","PeriodicalId":23957,"journal":{"name":"Transactions of the American Microscopical Society","volume":"74 1","pages":"22-33"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74158750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Rotifera, Vol. 1: Biology, Ecology and Systematics 轮虫，第1卷:生物学、生态学和系统学

Transactions of the American Microscopical Society Pub Date : 1994-01-01 DOI: 10.2307/3226585

R. Pennak, T. Nogrady, R. Wallace, T. Snell

引用次数: 145

Periodicity and the Influence of Temperature and Cellular Size in Contractile Vacuole Contraction Intervals 液泡收缩周期的周期性及温度和细胞大小的影响

Transactions of the American Microscopical Society Pub Date : 1993-10-01 DOI: 10.2307/3226564

Soroor Nematbakhsh, B. Bergquist

引用次数: 1