{"title":"[Replication and expression of plasmid pBR 322 during discontinuous culture of a stringent and relaxed Escherichia coli strain].","authors":"M Hecker, W Wiehle, A Schroeter, F Mach","doi":"10.1002/jobm.3630230606","DOIUrl":"https://doi.org/10.1002/jobm.3630230606","url":null,"abstract":"<p><p>The E. coli strains CP78 and CP79 carrying the plasmid pBR 322 display similar growth kinetics in discontinuous culture. During limitation of amino acids the stringent strain CP78 is able to synthesize guanosine-5'diphosphate-3'-diphosphate (ppGpp) and guanosine-5'-triphosphate-3' diphosphate, but the relaxed strain can not produce highly phosphorylated guanosine nucleotides. During the logarithmic phase of growth both strains contain similar amounts of plasmid DNA. During amino acid starvation plasmid DNA is amplified in the relaxed strain only, whereas in the stringent strain the plasmid content per cell remains constant. In stationary phase cells of CP78 a higher activity of plasmid-coded beta-lactamase than in CP79 cells was detected. Furthermore, remarkable differences between both strains were observed in the composition of proteins derived from the periplasmic fraction and separated by polyacrylamide gel electrophoresis. Our results might indicate a negative control of pBR 322 DNA replication by ppGpp during amino acid starvation.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 6","pages":"367-74"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17416391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of breeding stocks of the yeast Saccharomycopsis lipolytica by methods of moderate inbreeding.","authors":"C Kurischko, S G Inge-Vechtomov, H Weber","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Sporulation parameters of genetically labelled strains, derived from a wild strain of the alkane-utilizing yeast Saccharomycopsis lipolytica were improved by a breeding program using brother-sister crosses. Sporulation frequency, the number of four-spored asci and viability of ascospores could be significantly enhanced. To date a number of genetically well-defined strains is available that have good sporulation parameters and show a 1:1 segregation pattern of markers suitable for genetic analysis.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 8","pages":"513-5"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17421914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comparison of manganese oxidation by growing and resting cells of a freshwater bacterial isolate, strain FMn 1.","authors":"M A Zapkin, H L Ehrlich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A bacterial isolate, strain FMn 1, from reservoir sediment oxidized MN2+ when tested in growing culture and resting-cell suspension. The oxidation was biologically mediated and not the result of autoxidation because at a MnSO4 . H2O concentration of 0.05%, the pH remained below 7.5 for the duration of the experiments. The production of manganese oxide was qualitatively and quantitatively demonstrated. The manganese-oxidizing activity of this organism was found to be inducible.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 7","pages":"447-55"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17691990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Uncoupling of respiration in turimycin fermentations.","authors":"W. Effenberger, P. Müller, H. Bocker","doi":"10.1002/JOBM.19830230906","DOIUrl":"https://doi.org/10.1002/JOBM.19830230906","url":null,"abstract":"Results furnish evidence for a partial uncoupling of respiration at conditions of phosphate limitation in turimycin fermentations. The uncoupling was primarily caused by the high intracellular ATP hydrolase activity and probably also by the CN-resistant respiration. The results support the idea that high dephoshorylating enzyme activities may have a regulatory effect on the secondary product biosynthesis.","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"12 1","pages":"557-64"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86850180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comparison of manganese oxidation by growing and resting cells of a freshwater bacterial isolate, strain FMn 1.","authors":"M. A. Zapkin, H. Ehrlich","doi":"10.1002/JOBM.19830230712","DOIUrl":"https://doi.org/10.1002/JOBM.19830230712","url":null,"abstract":"A bacterial isolate, strain FMn 1, from reservoir sediment oxidized MN2+ when tested in growing culture and resting-cell suspension. The oxidation was biologically mediated and not the result of autoxidation because at a MnSO4 . H2O concentration of 0.05%, the pH remained below 7.5 for the duration of the experiments. The production of manganese oxide was qualitatively and quantitatively demonstrated. The manganese-oxidizing activity of this organism was found to be inducible.","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"132 1","pages":"447-55"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85179970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Molecular biology of the germination of Bacillus spores].","authors":"M Hecker","doi":"10.1002/jobm.3630230814","DOIUrl":"https://doi.org/10.1002/jobm.3630230814","url":null,"abstract":"<p><p>The review deals with recent results and problems of gene expression during germination of Bacillus spores. Three problems were selected: 1. The activation of metabolism as a prerequisite for the synthesis of nucleic acids and proteins. 2. The activation of nucleic acid and protein synthesis during germination. 3. The gene expression programme of germinating spores. Using the highly sensitive two-dimensional polyacrylamide gel analysis three major classes of proteins were distinguished, depending on the time of onset and duration of their syntheses: a) proteins made throughout germination (main class), b) proteins whose synthesis started only after a lag phase and then continued throughout germination, and c) proteins which are synthesized only during the early phases of germination. The programme of protein synthesis is an indicator for the control of gene expression during germination. The regulation of expression of these major gene groups during spore outgrowth is discussed.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 8","pages":"517-35"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17421915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Lysogeny and lysogenic conversion in methylotrophic bacteria. II. Lysogenic conversion in facultative methanol-assimilating Acetobacter strains].","authors":"L Wünsche, B Kiesel, H Fischer","doi":"10.1002/jobm.3630230307","DOIUrl":"https://doi.org/10.1002/jobm.3630230307","url":null,"abstract":"<p><p>The lysogenic state of the methylotrophic strain Acetobacter MB 58/1 is completely demonstrated by curing and lysogenization experiments. During these investigations we found that some phenotypic characteristics are modified by the presence or loss of the prophage MO 1. It could be shown that changes of the serological behaviour, the adsorption of the phages and the sensitivity against oxytetracycline are caused by lysogenic conversion. The phenotypic alterations of the bacterial cells induced by the phage genome are the result of modifications of the lipopolysaccharide structures on the cell surface. In the case of oxytetracycline resistance, interactions between the modified lipopolysaccharide structures and specific transport proteins of the cell membranes must be assumed.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 3","pages":"189-96"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17931381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Purification and isolation of the arom aggregates of Schizosaccharomyces pombe].","authors":"R Bode, G Kunze","doi":"10.1002/jobm.3630230503","DOIUrl":"https://doi.org/10.1002/jobm.3630230503","url":null,"abstract":"<p><p>The five enzymes that catalyzing steps two through six in the prechorismate polyaromatic amino acid biosynthetic pathway are physically associated and have been purified up to 400-fold from Schizosaccharomyces pombe. The native arom aggregate has a molecular weight of approx. 140,000-145,000 based on gel filtration, glycerol-density-gradient centrifugation, and polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. Similarities between the S. pombe arom aggregate and that of Neurospora crassa and Euglena gracilis are discussed.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 5","pages":"289-96"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17679166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of the autoregulator from Streptomyces griseus JA 5142 on surface cultures of blocked mutant ZIMET 43682.","authors":"U Gräfe, G Reinhardt, D Krebs, I Eritt, A Steudel","doi":"10.1002/jobm.3630230604","DOIUrl":"https://doi.org/10.1002/jobm.3630230604","url":null,"abstract":"<p><p>Zero time addition of the autoregulator (L-factor) from S. griseus (Lkm+Amy+) to surface cultures of its bald mutant ZIMET 43 682 (Amy-Lkm-) restored the capacity to form both anthracycline-type antibiotic leukaemomycin and aerial mycelium. The pertinent mycelia displayed the same growth rate and cellular levels of nucleic acids as the asporogeneous phenotype but the composition of fatty acids and phospholipids as well as the ratio of cytochromes b and c were altered. These differences indicate alterations in the cellular architecture of substrate and aerial hyphae. The results suggest that the autoregulator triggers the onset of a complex programme of differentiation at a very early stage.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"23 6","pages":"359-65"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17475996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}