Xenotransplantation最新文献_第4页

Incidence of serum antibodies to xenoantigens on triple-knockout pig cells in different human groups. 不同人类群体中三重基因敲除猪细胞上的异抗原血清抗体的发生率。

IF 3.9 4区医学

Xenotransplantation Pub Date : 2024-03-01 Epub Date: 2023-08-02 DOI: 10.1111/xen.12818

Songzhe He, Tao Li, Hao Feng, Jiaxiang Du, David K C Cooper, Hidetaka Hara, Hongtao Jiang, Dengke Pan, Gang Chen, Yi Wang

{"title":"Incidence of serum antibodies to xenoantigens on triple-knockout pig cells in different human groups.","authors":"Songzhe He, Tao Li, Hao Feng, Jiaxiang Du, David K C Cooper, Hidetaka Hara, Hongtao Jiang, Dengke Pan, Gang Chen, Yi Wang","doi":"10.1111/xen.12818","DOIUrl":"10.1111/xen.12818","url":null,"abstract":"Background: Xenoantigens other than Gal, Neu5Gc, and Sda may be playing a role in pig graft rejection. We investigated the incidence of antibodies to unknown pig xenoantigen in different human groups.Methods: We collected blood from TKO/hCD55 pigs (n = 3), and isolated PBMCs and RBCs. Serum samples were collected from (i) healthy human volunteers (n = 43), (ii) patients with end-stage renal disease (ESRD) (n = 87), (iii) the same patients after kidney allotransplantation (n = 50), and (iv) renal allotransplant recipients experiencing T cell-mediated rejection (allo-TCMR, n = 10). The sera were initially incubated with TKO/hCD55 pRBCs (1 × 108 cells) for 1 h to absorb anti-pig antibodies (except against SLA and possibly other antigens not expressed on pRBCs) and then the serum (absorbed or unabsorbed) was tested for antibody binding and complement-dependent cytotoxicity (CDC) to TKO/hCD55 pig PBMCs.Results: A significant reduction in IgM/IgG binding and CDC was observed in the absorbed sera. Serum obtained before and after renal allotransplantation showed no significant difference in IgM or IgG binding to, or in CDC of, TKO/hCD55 pig cells. IgM antibodies (but rarely IgG) against unknown xenoantigens expressed on TKO/hCD55 PBMCs, possibly against swine leukocyte antigens, were documented in healthy humans, patients with ESRD, and those with renal allografts undergoing acute T cell rejection. IgM (but not CDC) was higher in patients experiencing allo-TCMR.Conclusion: Human sera contain IgM antibodies against unknown pig xenoantigens expressed on TKO/hCD55 pPBMCs. Although not confirmed in the present study, the targets for these antibodies may include swine leukocyte antigens.","PeriodicalId":23866,"journal":{"name":"Xenotransplantation","volume":" ","pages":"e12818"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9922992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Barriers toward xenotransplantation in Arab World. 阿拉伯世界异种移植的障碍。

IF 3.9 4区医学

Xenotransplantation Pub Date : 2024-03-01 DOI: 10.1111/xen.12852

Maya Ghazi, Aalaa Saleh, Malak Abdallah, Diala El Masri, Jad El Masri, Lemir Majed El Ayoubi, Jihad Hawi, Abdo Jurjus

{"title":"Barriers toward xenotransplantation in Arab World.","authors":"Maya Ghazi, Aalaa Saleh, Malak Abdallah, Diala El Masri, Jad El Masri, Lemir Majed El Ayoubi, Jihad Hawi, Abdo Jurjus","doi":"10.1111/xen.12852","DOIUrl":"10.1111/xen.12852","url":null,"abstract":"Organ transplant is a crucial therapeutic strategy offering a life-saving and transformative medical intervention. It provides an opportunity to improve their quality of life and increase their lifespan. The shortage of organs remains a critical global challenge, leading to a prolonged waiting times for organ receivers, which contributes to an increase in morbidity and mortality rates. Hence, xenotransplantation offered a promising solution to the global shortage of organs through the use of animal organs, leading to an increase in donor availability, reducing waiting times, minimizing organ trafficking, improving genetic engineering advancements, and driving scientific innovation. Even though xenotransplantation has many benefits in the clinical setting, it has many barriers that are hindering its achievements and constraining its occurrence. Some barriers to xenotransplant are general, such as the immunological barrier, while others are specific to certain regions due to local causes. The Arab region exhibits disparities in clinical settings compared to the global context, marked by the huge economic crisis and a shortage of trained healthcare professionals. Considering the huge resources and advancements needed in the field of xenotransplantation, this review aims to explore the specific barriers toward xenotransplantation in the Arab countries, highlighting the challenges to overcome these barriers.","PeriodicalId":23866,"journal":{"name":"Xenotransplantation","volume":"31 2","pages":"e12852"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A murine groin site cardiac transplantation model-applicable tool for studying roles of peripheral lymph nodes in transplantation. 鼠腹股沟部位心脏移植模型--研究外周淋巴结在移植中作用的适用工具。

IF 3.9 4区医学

Xenotransplantation Pub Date : 2024-03-01 Epub Date: 2023-08-07 DOI: 10.1111/xen.12817

Yaguang Li, Wenjia Yuan, Mingda Zhong, Julia Qi, Xinguo Zheng, Xubiao Xie, Tengfang Li, Hedong Zhang, Xin Jiang, Longkai Peng, Helong Dai

{"title":"A murine groin site cardiac transplantation model-applicable tool for studying roles of peripheral lymph nodes in transplantation.","authors":"Yaguang Li, Wenjia Yuan, Mingda Zhong, Julia Qi, Xinguo Zheng, Xubiao Xie, Tengfang Li, Hedong Zhang, Xin Jiang, Longkai Peng, Helong Dai","doi":"10.1111/xen.12817","DOIUrl":"10.1111/xen.12817","url":null,"abstract":"The murine heterotopic cardiac transplantation model has been widely used to study antigen-specific immune responses or new immunosuppressive agents, which have a strong correlation with peripheral lymph nodes. Thus, a new organ transplantation model that is applicable to related studies is needed. Here, we describe a groin-site murine heart transplantation model using a cuff technique, in which the donor aorta and pulmonary artery are anastomosed to the truncated femoral vessels of the recipient. The mean survival time (MST) of the grafts in BALB/c-to-C57BL/6 allo-transplant group was 7.2 ± 0.3 days, and 1.9 ± 0.2 days in BALB/c-to-Sprague-Dawley (SD) rat xeno-transplant group. H&E results show that donor hearts from both groups demonstrate typical pathological features at the endpoint. Evans Blue tracing revealed that the popliteal lymph nodes of the grafted side hindlimb are larger than those of the contralateral side. Moreover, IHC staining for CD3, CD20 shows that the germinal center and cortex region of the grafted side of popliteal lymph nodes is apparently increased than that of the contralateral side. To sum up, this model may serve as an ideal model to study the role of peripheral lymph nodes in organ transplant rejection. In addition, extra-peritoneal grafting makes a step forward in animal welfare under the 3Rs' principle (Replacement, Reduction, Refinement).","PeriodicalId":23866,"journal":{"name":"Xenotransplantation","volume":" ","pages":"e12817"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10319342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term control of diabetes by tofacitinib-based immunosuppressive regimen after allo islet transplantation in diabetic rhesus monkeys that rejected previously transplanted porcine islets. 对之前移植猪胰岛产生排斥反应的糖尿病恒河猴进行异体胰岛移植后，使用基于托法替尼的免疫抑制方案可长期控制糖尿病。

IF 3.9 4区医学

Xenotransplantation Pub Date : 2024-03-01 DOI: 10.1111/xen.12850

Jong-Min Kim, Seong-Jun Kang, So-Hee Hong, Hyunwoo Chung, Jun-Seop Shin, Byoung-Hoon Min, Hyun Je Kim, Jongwon Ha, Chung-Gyu Park

{"title":"Long-term control of diabetes by tofacitinib-based immunosuppressive regimen after allo islet transplantation in diabetic rhesus monkeys that rejected previously transplanted porcine islets.","authors":"Jong-Min Kim, Seong-Jun Kang, So-Hee Hong, Hyunwoo Chung, Jun-Seop Shin, Byoung-Hoon Min, Hyun Je Kim, Jongwon Ha, Chung-Gyu Park","doi":"10.1111/xen.12850","DOIUrl":"10.1111/xen.12850","url":null,"abstract":"Porcine islet xenotransplantation has been highlighted as an alternative to allo islet transplantation. Despite the remarkable progress that has been made in porcine-islet pre-clinical studies in nonhuman primates, immunological tolerance to porcine islets has not been achieved to date. Therefore, allo islet transplantation could be required after the failure of porcine islet xenotransplantation. Here, we report the long-term control of diabetes by allogeneic pancreatic islet transplantation in diabetic rhesus monkeys that rejected previously transplanted porcine islets. Four diabetic male rhesus monkeys received the porcine islets and then allo islets (5700-19 000 IEQ/kg) were re-transplanted for a short or long period after the first xeno islet rejection. The recipient monkeys were treated with an immunosuppressive regimen consisting of ATG, humira, and anakinra for induction, and sirolimus and tofacitinib for maintenance therapy. The graft survival days of allo islets in these monkeys were >440, 395, >273, and 127, respectively, similar to that in allo islet transplanted cynomolgus monkeys that received the same immunosuppressive regimen without xeno sensitization. Taken together, it is likely that prior islet xenotransplantation does not affect the survival of subsequent allo islets under clinically applicable immunosuppressants.","PeriodicalId":23866,"journal":{"name":"Xenotransplantation","volume":"31 2","pages":"e12850"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140159084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Close contacts of xenograft recipients: Ethical considerations due to risk of xenozoonosis. 异种移植物接受者的密切接触者：异种动物传染病风险带来的伦理考虑。

IF 3.9 4区医学

Xenotransplantation Pub Date : 2024-03-01 DOI: 10.1111/xen.12847

Daniel J Hurst, Luz Padilla, Daniel Rodger, Tamar Schiff, David K C Cooper

{"title":"Close contacts of xenograft recipients: Ethical considerations due to risk of xenozoonosis.","authors":"Daniel J Hurst, Luz Padilla, Daniel Rodger, Tamar Schiff, David K C Cooper","doi":"10.1111/xen.12847","DOIUrl":"10.1111/xen.12847","url":null,"abstract":"With decades of pre-clinical studies culminating in the recent clinical application of xenotransplantation, it would appear timely to provide recommendations for operationalizing oversight of xenotransplantation clinical trials. Ethical issues with clinical xenotransplantation have been described for decades, largely centering on animal welfare, the risks posed to the recipient, and public health risks posed by potential spread of xenozoonosis. Much less attention has been given to considerations relating to potentially elevated risks faced by those who may care for or otherwise have close contact with xenograft recipients. This paper examines the ethical and logistical issues raised by the potential exposure to xenozoonotic disease faced by close contacts of xenotransplant recipients-defined herein as including but not limited to caregivers, household contacts, and sexual partners-which warrants special attention given their increased risk of exposure to infection compared to the general public. We discuss implications of assent or consent by these close contacts to potentially undergo, along with the recipient, procedures for infection screening and possible quarantine. We then propose several options and recommendations for operationalizing oversight of xenotransplantation clinical trials that could account for and address close contacts' education on and agency regarding the risk of xenozoonosis.","PeriodicalId":23866,"journal":{"name":"Xenotransplantation","volume":"31 2","pages":"e12847"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xenotransplantation at 30 years - A personal reminiscence and some thoughts for the future. 异种器官移植 30 年--个人回忆和对未来的一些思考。

IF 3.9 4区医学

Xenotransplantation Pub Date : 2024-03-01 DOI: 10.1111/xen.12846

David H Sachs

引用次数: 0

Evaluation of knowledge, attitude, and awareness of liver transplant patients toward xenotransplantation 评估肝移植患者对异种移植的知识、态度和认识

IF 3.9 4区医学

Xenotransplantation Pub Date : 2024-02-26 DOI: 10.1111/xen.12844

Bahar Aslan Dogan, Gurkan Ozden, Sevim Dolu, Mesut Mese, Sami Akbulut

引用次数: 0

A study of knowledge and acceptance of kidney xenotransplantation among Chinese kidney transplant recipients and candidates 关于中国肾移植受者和候选者对肾脏异种移植的了解和接受程度的研究

IF 3.9 4区医学

Xenotransplantation Pub Date : 2024-02-26 DOI: 10.1111/xen.12843

Yuhao Tu, Xiangli Zhao, Gang Chen, Lan Zhu

引用次数: 0

Pressing ethical issues relating to clinical pig organ transplantation studies 与临床猪器官移植研究有关的紧迫伦理问题

IF 3.9 4区医学

Xenotransplantation Pub Date : 2024-02-26 DOI: 10.1111/xen.12848

Daniel J. Hurst, David K. C. Cooper

引用次数: 0

Hemodynamics in pig-to-baboon heterotopic thoracic cardiac xenotransplantation: Recovery from perioperative cardiac xenograft dysfunction and impairment by cardiac overgrowth 猪-狒狒异位胸腔心脏异种移植的血液动力学：从围术期心脏异种移植的功能障碍和心脏过度生长的损害中恢复过来

IF 3.9 4区医学

Xenotransplantation Pub Date : 2024-01-25 DOI: 10.1111/xen.12841

Martin Bender, Alessandro Panelli, Bruno Reichart, Julia Radan, Maren Mokelke, Elisabeth Neumann, Ines Buttgereit, Sebastian Michel, Andreas Bauer, Ann Kathrin Fresch, Tanja Mayr, Fabian Werner, Stefanie Egerer, Andrea Bähr, Barbara Kessler, Nikolai Klymiuk, David Ayares, Eckhard Wolf, Christian Hagl, Paolo Brenner, Matthias Längin, Jan-Michael Abicht

引用次数: 0