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Zeitschrift fur klinische Chemie und klinische Biochemie最新文献

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A simple method for clearing lipemic sera by lipid extraction. 脂质提取清除血脂血清的简单方法。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01
G Hillmann
{"title":"A simple method for clearing lipemic sera by lipid extraction.","authors":"G Hillmann","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"327-8"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11389912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bound and free light chains in subacute sclerosing panencephalitis and multiple sclerosis serum and cerebrospinal fluid. 亚急性硬化性全脑炎和多发性硬化血清和脑脊液中的结合和游离轻链。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01 DOI: 10.1515/cclm.1975.13.7.305
F Bollengier, A Lowenthal, W Henrotin
{"title":"Bound and free light chains in subacute sclerosing panencephalitis and multiple sclerosis serum and cerebrospinal fluid.","authors":"F Bollengier,&nbsp;A Lowenthal,&nbsp;W Henrotin","doi":"10.1515/cclm.1975.13.7.305","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.7.305","url":null,"abstract":"<p><p>The kappa-lambda light chain ratios, the presence of free light chains and the double ring formation, with antikappa and antilambda serum, in single radial immunodiffusion were investigated in serum and cerebrospinal fluid of patients with subacute sclerosing panencephalitis. Cerebrospinal fluid samples of several multiple sclerosis cases were considered simultaneously. The results obtained suggest special immunoglobulin synthesis in both diseases.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"305-10"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.7.305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11998680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 34
Serum dopamine beta-hydroxylase: assay and enzyme properties. 血清多巴胺-羟化酶:测定和酶的性质。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01 DOI: 10.1515/cclm.1975.13.7.273
E S Markianos, I E Nyström
{"title":"Serum dopamine beta-hydroxylase: assay and enzyme properties.","authors":"E S Markianos,&nbsp;I E Nyström","doi":"10.1515/cclm.1975.13.7.273","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.7.273","url":null,"abstract":"<p><p>A method for the estimation of dopamine beta-hydroxylase activity in human serum is described, based on a thin layer chromatographic separation of the substrate ([14C]tyramine) from the reaction product ([14C]octopamine). The basic properties of the human serum enzyme, investigated by this method are described.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"273-6"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.7.273","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11458258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Ciliotoxicity of cigarette smoke and adenylate kinase. 香烟烟雾和腺苷酸激酶对纤毛的毒性。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01
H Mattenheimer
{"title":"Ciliotoxicity of cigarette smoke and adenylate kinase.","authors":"H Mattenheimer","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"325-6"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12414695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Errors, resulting from storage, in the determination of 11 parameters in heparinized whole blood and plasma (author's transl)]. 【在测定肝素化全血和血浆中的11个参数时，由于储存导致的误差(作者译)】。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-06-01
H Keller
{"title":"[Errors, resulting from storage, in the determination of 11 parameters in heparinized whole blood and plasma (author's transl)].","authors":"H Keller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Eleven parameters in heparinzed whole blood and plasma samples were investigated for storage-dependent changes. Samples were stored at +25 degrees C, and analyses were performed with the SMA 12/60 at 0, 24, 48, 72 and 96 hours after sampling. In whole blood, the following parameters increased significantly during storage: potassium calcium, phosphate, total protein. The following decreased: chloride, CO2, uric acid and bilirubin. In plasma samples, analogous effects were observed for: calcium, CO2, total protein, uric acid and bilirubin.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 6","pages":"217-24"},"PeriodicalIF":0.0,"publicationDate":"1975-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12387732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Desmosterol accumulation in rats with experimental myotonia. 实验性肌强直大鼠的去氨甾醇积累。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-06-01 DOI: 10.1515/cclm.1975.13.6.225
D Seiler, W Fiehn, E Kuhn
{"title":"Desmosterol accumulation in rats with experimental myotonia.","authors":"D Seiler,&nbsp;W Fiehn,&nbsp;E Kuhn","doi":"10.1515/cclm.1975.13.6.225","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.6.225","url":null,"abstract":"Desmosterol is found in various organs of rats that show signs of myotonia in their skeletal muscle as a result of treatment with 20.25-diazacholesterol. The amount of desmosterol depends on the time of treatment, and is different in different organs and different kinds of muscle. The increase in desmosterol is much lower and the rats do not show any signs of myotonia when fed a cholesterol rich diet in addition to treatment with 20.25-diazacholesterol. Treatment with triparanol also causes desmosterol accumulation but in these rats myotonia is rarely observed. Our results suggest that in the experimental animals myotonia becomes manifest when every second cholesterol molecule of the muscle cell membrane is replaced by desmosterol. This is easily achieved in animals fed with 20.25-diazacholesterol but rarely occurs with triparanol.","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 6","pages":"225-9"},"PeriodicalIF":0.0,"publicationDate":"1975-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.6.225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12387733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
[Detection of a hitherto unknown ninhydrin positive substance in the haemolysate of patients with metachromatic leucodystrophy (author's transl)]. 【在异色性白质营养不良患者的血液中检测到一种迄今未知的茚三酮阳性物质(作者译)】。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-06-01
G W Schmidt
{"title":"[Detection of a hitherto unknown ninhydrin positive substance in the haemolysate of patients with metachromatic leucodystrophy (author's transl)].","authors":"G W Schmidt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A hitherto unknown substance, which appears between glycine and alanine in ion exchange chromatograms, was found in the erythrocyte cold haemolysate of two children with metachromatic leucodystophy; this substance could not be detected in the haemolysates from patients with other (brain) diseases. It would be worthwhile to test for this symptom in confirmed, still active (florid) cases of metachromatic leucodystrophy.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 6","pages":"239-40"},"PeriodicalIF":0.0,"publicationDate":"1975-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12416579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Quantitation of lipids in blood, plasma and tissues by gas-liquid chromatography (author's transl)]. [气液色谱法测定血液、血浆和组织中的脂质(作者译)]。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-06-01
M Radke, H Schäfer, J D Kruse-Jarres
{"title":"[Quantitation of lipids in blood, plasma and tissues by gas-liquid chromatography (author's transl)].","authors":"M Radke,&nbsp;H Schäfer,&nbsp;J D Kruse-Jarres","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 6","pages":"231-7"},"PeriodicalIF":0.0,"publicationDate":"1975-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12387734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The influence of pyridoxal 5'-phosphate on the temperature relationships of aspartate aminotransferase isoenzymes]. [吡哆醛5′-磷酸对天冬氨酸转氨酶同工酶温度关系的影响]。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-05-01
K Jung, B Lüdtke, E Egger
{"title":"[The influence of pyridoxal 5'-phosphate on the temperature relationships of aspartate aminotransferase isoenzymes].","authors":"K Jung,&nbsp;B Lüdtke,&nbsp;E Egger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The relationship between temperature and the behaviour of aspartate aminotransferase was investigated in the presence of pyridoxal 5'-phosphate. The addition in vitro of pyridoxal 5'-phosphate caused an increase in the activity and altered the thermal behaviour of aspartate aminotransferase. In choosing the temperature for the determination of enzymic activity, the concentration of the coenzyme must therefore also be considered.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 5","pages":"179-81"},"PeriodicalIF":0.0,"publicationDate":"1975-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12386851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The determination of chloride in plasma and serum (mercury(II)-thiocyanate method) with the Greiner Electronic Selective Analyzer GSA II (author's transl)]. [用Greiner电子选择分析仪GSA II测定血浆和血清中的氯化物(汞(II)-硫氰酸盐法)[作者简介]。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-05-01
H Küffer, R Richterich, R Kraft, E Peheim, J P Colombo
{"title":"[The determination of chloride in plasma and serum (mercury(II)-thiocyanate method) with the Greiner Electronic Selective Analyzer GSA II (author's transl)].","authors":"H Küffer,&nbsp;R Richterich,&nbsp;R Kraft,&nbsp;E Peheim,&nbsp;J P Colombo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>1. A mercury(II)-thiocyanate method for the determination of chloride in plasma and serum was adapted for the Greiner Electronic Selective Analyzer GSA II. A sample blank value and a partial reagent blank value were determined by omitting thiocyanate from the control system. 2. The course of the reaction was investigated. For a reaction time of 350-500 s, the response was linear between 30 and 130 mmol/l. Between 90 and 110 mmol/l, the deviation between the actual and the theoretical value is less than 1%. 3. The calibration must be checked and, if necessary, restandardized; this is probably due to variable contamination of the reagents with chloride ions. 4. Haemolysis, lipaemia and bilirubin do not interfere. Protein has no effect on the course of the reaction. 5. At concentrations around 100 mmol/l, the in series precision, expressed as the variation coefficient (%), is 0.3-0.6% for aqueous solutions, 0.4-0.8% for liquid control sera, and 0.8-1.5% for lyophilized control sera. 6. No carry over was detectable from samples containing 150 to those containing 10 mmol/l.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 5","pages":"203-11"},"PeriodicalIF":0.0,"publicationDate":"1975-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12387730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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