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Zeitschrift fur klinische Chemie und klinische Biochemie最新文献

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Proceedings: The effects of adducts and the nature of activated oxygen for cytochrome P-450 catalyzed hydroxylation reactions. 研究进展:加合物和活性氧对细胞色素P-450催化羟基化反应的影响。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-08-01
W Estabrook, J Werringloer, P J O'Brien, J A Peterson
{"title":"Proceedings: The effects of adducts and the nature of activated oxygen for cytochrome P-450 catalyzed hydroxylation reactions.","authors":"W Estabrook, J Werringloer, P J O'Brien, J A Peterson","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 8","pages":"373-4"},"PeriodicalIF":0.0,"publicationDate":"1975-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12392374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[In memoriam: Guenther Hillmann]. [纪念:冈瑟·希尔曼]。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01
J Büttner, E Schütte
{"title":"[In memoriam: Guenther Hillmann].","authors":"J Büttner, E Schütte","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"329-30"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12284281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The diagnostic significance of liver cell inhomogeneity: serum enzymes in patients with central liver necrosis and the distribution of glutamate dehydrogenase in normal human liver. 肝细胞不均匀性的诊断意义:中枢性肝坏死患者血清酶及正常人肝脏谷氨酸脱氢酶的分布
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01 DOI: 10.1515/cclm.1975.13.7.311
W G Guder, A Habicht, J Kleissl, U Schmidt, O H Wieland
{"title":"The diagnostic significance of liver cell inhomogeneity: serum enzymes in patients with central liver necrosis and the distribution of glutamate dehydrogenase in normal human liver.","authors":"W G Guder,&nbsp;A Habicht,&nbsp;J Kleissl,&nbsp;U Schmidt,&nbsp;O H Wieland","doi":"10.1515/cclm.1975.13.7.311","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.7.311","url":null,"abstract":"<p><p>16 Patients with acute right-sided cardiac failure associated with a high pressure of the central venous system, exhibited a marked increase in glutamate dehydrogenase activity in serum. This increase was 40-fold higher than in patients with acute viral hepatitis. Histological examination of seven deceased patients revealed central necrosis within the liver lobule. This observation led us to determine glutamate dehydrogenase activity in microdissected peripheral and central portions from the unchanged liver lobule. A 1.7-fold higher glutamate dehydrogenase activity was found in the central part of the liver lobule than in the peripheral portion. The diagnostic significance of the glutamate dehydrogenase activity distribution along the cords of liver cells is discussed in view of liver diseases with central necrosis.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"311-8"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.7.311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12365977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 42
[A simplified method for the fluorometric determination of 5-hydroxy-indoleacetic acid in the human liquor cerebrospinalis (author's transl)]. [人脑脊液中5-羟基吲哚乙酸的简化荧光测定方法[作者译]。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01
F Geissbühler
{"title":"[A simplified method for the fluorometric determination of 5-hydroxy-indoleacetic acid in the human liquor cerebrospinalis (author's transl)].","authors":"F Geissbühler","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The fluorometric analysis of 5-hydroxy-indoleacetic acid by condensation with o-phthaldialdehyde has been modified by substituting a HCl-H2SO4 mixture at room temperature for hot concentrated HCl. The sensitivity of the method was increased by a factor of 2.7.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"283-4"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12365024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A combined adsorption-gel filtration technique for the determination of the cortisol-binding capacity of transcortin. 联合吸附-凝胶过滤技术测定转质与皮质醇的结合能力。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01 DOI: 10.1515/cclm.1975.13.7.291
U Schwartz, J Hammerstein
{"title":"A combined adsorption-gel filtration technique for the determination of the cortisol-binding capacity of transcortin.","authors":"U Schwartz,&nbsp;J Hammerstein","doi":"10.1515/cclm.1975.13.7.291","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.7.291","url":null,"abstract":"<p><p>A combined adsorption-gel filtration technique has been developed for the quantitation of the cortisol-binding capacity of transcortin: Endogenous steroids are removed from plasma by adsorption on uncoated charcoal. Saturation of the \"stripped\" binding sites of transcortin is accomplished by equilibrating the sample with a definite amount of labeled cortisol of low specific activity (0.1 muCi/mug). Transcortin-bound [4-14C]cortisol is isolated by gel filtration over Sephadex G-50 at 4 degrees C and measured by liquid scintillation counting. The cortisol-binding capacity of transcortin is calculated directly on the basis of the known specific activity of cortisol. The modification described eliminates methodological disadvantages associated with the original gel filtration procedures, i.e. the possible interference of various endogenous steroids with cortisol binding to transcortin, and the necessity of fluorometric or colorimetric determination of protein-bound cortisol. The values of the cortisol-binding capacity of transcortin in plasma obteined by this simplified assay are in close agreement with results reported in the literature (mean +/- S.D.): healthy males 261 +/- 23 mug/l) of transcortin-bound cortisol (n = 13), healthy nonpregnant females 255 +/- 31 mug/l (n = 15), and pregnant females prior to delivery 560 +/- 82 mug/l (n = 12).</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"291-7"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.7.291","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12365025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
The effect of fluorocarbon FC 43 on the metabolism of steroids during perfusion of the isolated rat liver. 氟碳FC 43对离体大鼠肝脏灌注过程中甾体代谢的影响。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01 DOI: 10.1515/cclm.1975.13.7.319
M Höller, H Breuer
{"title":"The effect of fluorocarbon FC 43 on the metabolism of steroids during perfusion of the isolated rat liver.","authors":"M Höller,&nbsp;H Breuer","doi":"10.1515/cclm.1975.13.7.319","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.7.319","url":null,"abstract":"<p><p>The influence of fluorocarbon FC 43 on the metabolism of oestrogens in the isolated perfused rat liver was investigated. In comparative once-through perfusions with FC 43 emulsion or albumin solution as perfusion medium, the clearance rates of oestrone and its metabolites were determined. In perfusions with FC 43, the clearance of oestrone was lower, and the metabolites formed in the liver were more concentrated in the outflowing medium than in perfusions without fluorocarbon. This can be explained by the high affinity, even of conjugated oestrogens, to FC 43, which is established by equilibrium dialysis and partition coefficient. The results presented here show that the fluorocarbon has a strong influence of its own on the metabolism of steroids in the isolated perfused liver. Therefore, this solvent should be avoided as medium when the metabolism of steroids is studied in perfusion experiments.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"319-23"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.7.319","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12365978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Serum alpha-N-acetylglucosaminidase: determination, characterization, and corrective activity in Sanifilippo B fibroblasts. 血清α - n -乙酰氨基葡萄糖酶:Sanifilippo B成纤维细胞的测定、表征和纠正活性。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01 DOI: 10.1515/cclm.1975.13.7.285
K von Figura, M Lögering, H Kresse
{"title":"Serum alpha-N-acetylglucosaminidase: determination, characterization, and corrective activity in Sanifilippo B fibroblasts.","authors":"K von Figura,&nbsp;M Lögering,&nbsp;H Kresse","doi":"10.1515/cclm.1975.13.7.285","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.7.285","url":null,"abstract":"<p><p>Assays for the determination of serum alpha-N-acetylglucosaminidase (EC 3.2.1.50) activity are described employing p-nitrophenyl-N-acetyl-alpha-D-glucosaminide, phenyl-N-acetyl-alpha-D-glucosaminide, and UDP-N-acetylglucosamine as substrates. A log normal distribution of the serum enzyme activity was found. The determination of serum alpha-N-acetylglucosaminidase activity proved to be a valuable tool for the recognition of homozygous and heterozygous carriers of the Sanfilippo B gene.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"285-9"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.7.285","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11458259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
A review of radioimmunoassay for steroids. 类固醇放射免疫分析法综述。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01 DOI: 10.1515/cclm.1975.13.7.261
E Nieschlag, E J Wickings
{"title":"A review of radioimmunoassay for steroids.","authors":"E Nieschlag,&nbsp;E J Wickings","doi":"10.1515/cclm.1975.13.7.261","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.7.261","url":null,"abstract":"<p><p>This review, based on the current literature, considers the practical aspects of steroid radioimmunoassays. The problems associated with the raising of specific antisera and their characterization are discussed. Features of assay design, reliability criteria and practicability of radioimmunoassays for steroids are considered.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"261-71"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.7.261","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12365022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
The mechanism of the increase in the activity of liver alkaline phosphatase in experimental cholestasis: measurement of an increased enzyme concentration by immunochemical titration. 实验性胆汁淤积肝碱性磷酸酶活性升高的机制:免疫化学滴定法测定酶浓度升高。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01 DOI: 10.1515/cclm.1975.13.7.277
R Schlaeger
{"title":"The mechanism of the increase in the activity of liver alkaline phosphatase in experimental cholestasis: measurement of an increased enzyme concentration by immunochemical titration.","authors":"R Schlaeger","doi":"10.1515/cclm.1975.13.7.277","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.7.277","url":null,"abstract":"<p><p>Alkaline phosphatase (EC 3.1.3.1) activity increases 5-15 fold in the livers of rats, following bile duct ligation. The mechanism of this increase has been the subject of numerous investigations. In HeLa cells the synthesis of a different phosphatase enzyme protein with higher catalytic activity has been postulated. After preparing an antiserum against rat liver phosphatase, we compared the phosphatase protein concentration in normal and cholestatic livers by immunochemical titration. Our results clearly indicate that the elevation of enzyme activity is due to an increased accumulation of enzyme protein.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"277-81"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.7.277","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12365023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Separation of the coprophorphyrin isomers I and III by thin-layer chromatography. 用薄层色谱法分离共卟啉I和III异构体。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-07-01 DOI: 10.1515/cclm.1975.13.7.299
E Schermuly, M Doss
{"title":"Separation of the coprophorphyrin isomers I and III by thin-layer chromatography.","authors":"E Schermuly,&nbsp;M Doss","doi":"10.1515/cclm.1975.13.7.299","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.7.299","url":null,"abstract":"<p><p>An improved, specific and sensitive method for the determination of the coproporphyrin isomers I and III is described. In this method, the hydrolysis of the coproporphyrin methyl esters takes place in the silica gel layer of acid-resistant pre-coated thin layer plates. After chromatography in the solvent system 2,6-dimethylpyridine/water, (volume ratio 20 ml + 6 ml), in an ammonia atmosphere, the isomers are measured, either directly in the silica gel layer by fluorimetry, or after elution in 1.0 mol/l hydrochloric acid. The technique makes analysis in the nanogram range possible. See article.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 7","pages":"299-304"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.7.299","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12365976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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