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Zeitschrift fur klinische Chemie und klinische Biochemie最新文献

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[The enzymic and immunological determination of coeruloplasmin: sex- and method-dependent differences during the administration of oestrogens (author's transl)]. [蓝蛋白的酶学和免疫学测定:雌激素给药期间的性别和方法依赖性差异[作者译]。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-10-01
H Ebeling
{"title":"[The enzymic and immunological determination of coeruloplasmin: sex- and method-dependent differences during the administration of oestrogens (author's transl)].","authors":"H Ebeling","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>After 14 days of 1 mug ethinyloestradiol per kg body weight per day, both sexes show highly significant increases in serum coeruloplasmin which are independent of the determination method. Expressed on a percentage basis, the concentrations of coeruloplasmin under oestrogen medication determined by the p-phenylendiamine oxidase reaction are significantly higher than those determined by the immunological-nephelometric method. This is because several coeruloplasmin components, mainly C and DO, take part in the determination with p-phenylendiamine oxidase, whereas the immunological method measures chiefly the coeruloplasmin DO component. The significant sex differences under oestrogen medication shown by the determination with p-phenylendiamine oxidase, can therefore be explained by the presence of C- and D-coeruloplasmins in women, while the serum of men contains almost exclusively D-coeruloplasmins. Under oestrogen medication, the increase occurs primarily in the DO- coeruloplasmin component, and women also show an increase in coeruloplasmin-C. The significant higher enzymatic concentrations in the serum of the women in contrast to the men are thus plausibly explained. With the immunological assay, the sex differences were only probably significant.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 10","pages":"445-51"},"PeriodicalIF":0.0,"publicationDate":"1975-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12000160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Simultaneous determination of 5'-nucleotidase and alkaline phosphatase activities in serum. 血清5′-核苷酸酶和碱性磷酸酶活性的同时测定。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-10-01 DOI: 10.1515/cclm.1975.13.10.453
A A El-Aaser, M M El-Merzabani
{"title":"Simultaneous determination of 5'-nucleotidase and alkaline phosphatase activities in serum.","authors":"A A El-Aaser,&nbsp;M M El-Merzabani","doi":"10.1515/cclm.1975.13.10.453","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.10.453","url":null,"abstract":"<p><p>A simple method is described for the simultaneous determination of alkaline phosphatase (EC 3.1.3.1) and 5'-nucleotidase (EC 3.1.3.5) in serum. The method is based on the determination of inorganic phosphorus released by the action of the two enzymes on adenosine-5'-monophosphate at pH 9.5 (200 mmol/l tris-buffer) in the presence and absence of L-cysteine. This amino acid at a concentration of 2--10 mmol/l was found to be a specific inhibitor for alkaline phosphatase but with no effect on 5'-nucleotidase activity.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 10","pages":"453-9"},"PeriodicalIF":0.0,"publicationDate":"1975-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.10.453","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11225281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 31
[New protease-inhibitors with broad specificity in the polychaet Sabellastarte indica (Savingny), I (author's transl)]. [在印度多毛线虫中具有广泛特异性的新蛋白酶抑制剂(Savingny)， I(作者翻译)]。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-10-01
C Gauwerky, G Corman, G Uhlenbruck
{"title":"[New protease-inhibitors with broad specificity in the polychaet Sabellastarte indica (Savingny), I (author's transl)].","authors":"C Gauwerky,&nbsp;G Corman,&nbsp;G Uhlenbruck","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this paper we describe new and so far unknown protease inhibitors present in the tentacles of the annelid Sabellastarte indica Savingny. At least five different isoinhibitors with inhibitory activity towards trypsin, plasmin, chymotrypsin and kallikrein can be separated electrophoretically. Our protease inhibitor active material differs from the other well known protease inhibitors found in invertebrates in its high molecular weight, in that it is heat-labile and in the occurrence of the isoelectric point in the weakly acid region. On the other hand, the new protease inhibitors have some similarities to the soybean inhibitor described by Kunitz, and to ovomucoid. We also discuss the possibility that these inhibitors may influence the fibrinolytic system.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 10","pages":"429-35"},"PeriodicalIF":0.0,"publicationDate":"1975-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11347735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quality control in hematology by means of values from patients. 血液学的质量控制，从病人的价值。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-09-01 DOI: 10.1515/cclm.1975.13.9.395
W P Rutten, R J Scholtis, N A Schmidt, R J van Oers
{"title":"Quality control in hematology by means of values from patients.","authors":"W P Rutten,&nbsp;R J Scholtis,&nbsp;N A Schmidt,&nbsp;R J van Oers","doi":"10.1515/cclm.1975.13.9.395","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.9.395","url":null,"abstract":"<p><p>The results of the analyses which were obtained with the Hemalog for the determination of platelets, white blood cells, red blood cells, hemoglobin and hematocrit were statistically analysed for the quality control of the determinations. Now that the testing method has been applied for 6 months, it appears from the results that it is extremely suitable for the determination of hemoglobin, red blood cells and the hematocrit and, to a lesser extent, for white blood cells and platelets.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 9","pages":"395-400"},"PeriodicalIF":0.0,"publicationDate":"1975-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.9.395","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12375960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
On the fluorimetric determination of oestriol in pregnancy urines after thin layer chromatography. 薄层色谱法测定妊娠尿中雌三醇含量。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-09-01 DOI: 10.1515/cclm.1975.13.9.381
M van Bezeij, M W Bosch
{"title":"On the fluorimetric determination of oestriol in pregnancy urines after thin layer chromatography.","authors":"M van Bezeij,&nbsp;M W Bosch","doi":"10.1515/cclm.1975.13.9.381","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.9.381","url":null,"abstract":"<p><p>An improved method is described for the determination of oestriol concentrations in urines of pregnant women. Oestriol is detected as a derivate of 1-dimethylaminonaphtalene-5-sulphonylchloride (DANSYLchloride). The method has the following characteristics: 1. The recovery of oestriol-16-glucuronide is 94%. 2. For the interpretation of the results obtained with this method mean values and 95% probability bounds of urinary oestriol excretion for each week of the last three months of pregnancy are given. (e.g. 49--125 mumol/24 h at 32 weeks of gestation, 108--238 mumol/24 h at 40 weeks). 3. The coefficient of variation of the method is 4% (from day to day). 4. The lowest detectable concentration is 3 mumol/l. 5. Influence of glucose can be eliminated. 6. Other urinary compounds have no influence on the determination.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 9","pages":"381-6"},"PeriodicalIF":0.0,"publicationDate":"1975-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.9.381","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12375958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A systematic investigation on the Hemalog. 血液学的系统研究。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-09-01 DOI: 10.1515/cclm.1975.13.9.387
W P Rutten, R J Scholtis, N A Schmidt, R J van Oers
{"title":"A systematic investigation on the Hemalog.","authors":"W P Rutten,&nbsp;R J Scholtis,&nbsp;N A Schmidt,&nbsp;R J van Oers","doi":"10.1515/cclm.1975.13.9.387","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.9.387","url":null,"abstract":"The Hemalog system is an automated hematological instrument. From one sample this instrument determines the value for platelets (PBC), white blood cells (WBC), red blood cells (RBC), hemoglobin (Hb) and the hematocrit simultaneously. Moreover the system calculates the derived values: the mean cell volume (MCV), mean cell hemoglobin (MCH) and the mean cell hemoglobin concentration (MCHC). The values were investigated for cross sample contamination, coincidence, reproducibility, and compared with other methods. From this comparative study we can conclude that the Hemalog is a suitable and reliable instrument for hematological determination.","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 9","pages":"387-93"},"PeriodicalIF":0.0,"publicationDate":"1975-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.9.387","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12375959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
[Quantitative determination of enteral calcium resorption with enriched stable 46Ca (author's transl)]. [富集稳定型46Ca定量测定肠内钙吸收[作者简介]。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-09-01
M Persigehl, K Kasperek, F Ritzl, A Höck, L E Feinendegen
{"title":"[Quantitative determination of enteral calcium resorption with enriched stable 46Ca (author's transl)].","authors":"M Persigehl,&nbsp;K Kasperek,&nbsp;F Ritzl,&nbsp;A Höck,&nbsp;L E Feinendegen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The application of a Ca-resportion test without the use of radioisotopes, which can therefore be used for babies, children and pregnant women, was investigated on 14 adult patients. In place of a radioisotope, an enriched form of the stable isotope 46Ca was administered orally, and the proportion of the applied dose of 46Ca appearing in the serum and urine was measured. 46Ca was measured by neutron activation over 47Sc. The clinical picture showed a close correlation with the determined resorption capacities. On the basis of the results, patients were classified into three groups: those with normal, increased, and decreased Ca-resorption.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 9","pages":"417-21"},"PeriodicalIF":0.0,"publicationDate":"1975-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12375963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A microelectrophoretic method for the separation of beta-N-acetylglucosaminidase A and B from cultured human fibroblasts and amniotic cells with the aid of polyacrylamide flat disc gels. 用聚丙烯酰胺平盘凝胶从培养的人成纤维细胞和羊膜细胞中分离β - n -乙酰氨基葡萄糖酶A和B的微电泳方法
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-09-01 DOI: 10.1515/cclm.1975.13.9.413
S Singh, H W Goedde
{"title":"A microelectrophoretic method for the separation of beta-N-acetylglucosaminidase A and B from cultured human fibroblasts and amniotic cells with the aid of polyacrylamide flat disc gels.","authors":"S Singh,&nbsp;H W Goedde","doi":"10.1515/cclm.1975.13.9.413","DOIUrl":"https://doi.org/10.1515/cclm.1975.13.9.413","url":null,"abstract":"","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 9","pages":"413-5"},"PeriodicalIF":0.0,"publicationDate":"1975-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.9.413","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12000161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Micro method for the selective assay of unesterified long chain fatty acids in serum (author's transl)]. [微法选择性测定血清中未酯化长链脂肪酸(作者译)]。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-09-01
V A Grünert
{"title":"[Micro method for the selective assay of unesterified long chain fatty acids in serum (author's transl)].","authors":"V A Grünert","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A microanalytical method is reported for the selective and direct determination of low concentrations of unesterified fatty acids in serum. A new procedure is used to form derivatives of the fatty acids with alkyl iodide, which are separated over a solid phase of K2CO3. The assay was developed for the qualitative and quantitative determination of unesterified fatty acids mobilized from fat tissue in defined metabolic states.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 9","pages":"407-12"},"PeriodicalIF":0.0,"publicationDate":"1975-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12375962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A comparative study of total protein and albumin in man, monkey, dog and rat employing different analytical methods (author's transl)]. [不同分析方法对人、猴、狗和大鼠总蛋白和白蛋白的比较研究[作者简介]。
Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1975-09-01
A Metz, A Schütze
{"title":"[A comparative study of total protein and albumin in man, monkey, dog and rat employing different analytical methods (author's transl)].","authors":"A Metz,&nbsp;A Schütze","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the present study, serum albumin was determined colorimetrically in three animal species and in man with the help of four currently used dye-reagents (protein binding reagents). The results were compared with those obtained from corresponding electrophoretic and biuret determinations, using Versatol as a standard-control throughout. Using 2-(4-hydroxyazobenzene)-benzoic acid and bromocresol purple as reagent for the albumin determinations, species-specific differences from the electrophoretic results were found. No such differences occurred with bromo-cresol green, except in rats. There was no significant difference in albumin concentrations between man, monkeys and rats. Total protein concentration was only similar in man and monkeys.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 9","pages":"423-6"},"PeriodicalIF":0.0,"publicationDate":"1975-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12000162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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