Yi chuan xue bao = Acta genetica Sinica最新文献_第6页

[Identification of differential genomic genes of Mycobacterium tuberculosis H37Rv and attenuated strain H37Ra by suppression subtractive hybridization]. [抑制减法杂交鉴定结核分枝杆菌H37Rv与减毒菌株H37Ra的差异基因组基因]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-09-01

Zhi-Hong Xiong, Yu-Hui Zhuang, Guo-Li Li

引用次数: 0

[Establishment of transgenic mouse line skin-specifically expressing hCTLA4-Ig]. [建立皮肤特异性表达hCTLA4-Ig的转基因小鼠系]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-09-01

Yong Wang, Feng-Chao Wang, Hong Wei, Yong Ni, Jun Wu, Xiang Gao

引用次数: 0

[Methodology of mapping quantitative trait loci for discrete traits using maximum likelihood]. [使用最大似然方法为离散性状绘制数量性状位点的方法]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-09-01

Zong-Jun Yin, Qin Zhang, Hong-Quan Chen, Ji-Gang Zhang, Xiang-Dong Ding, Chun-Kao Wang

引用次数: 0

Molecular evidence of barley yellow dwarf virus replication/movement suppressed by the resistance gene Bdv2. 抗基因Bdv2抑制大麦黄矮病毒复制/运动的分子证据

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-09-01

Xiao-Dong Liu, Zeng-Yan Zhang, Yan Liu, Zhi-Yong Xin

{"title":"Molecular evidence of barley yellow dwarf virus replication/movement suppressed by the resistance gene Bdv2.","authors":"Xiao-Dong Liu, Zeng-Yan Zhang, Yan Liu, Zhi-Yong Xin","doi":"","DOIUrl":"","url":null,"abstract":"A wheat-Thinopyrum intermedium translocation line YW642 possesses the resistance to GAV serotype of barley yellow dwarf virus (BYDV), in which the resistance gene Bdv2 is derived from the chromosome 7X of Thinopyrum intermedium group 7. It is interesting to analyze BYDV accumulation content in the resistant and susceptible wheat plants for controlling BYDV disease and understanding the resistance mechanism against BYDV. In the paper, semi-quantitative reverse-transcription PCR (RT-PCR) was used to detect and quantify BYDV-GAV in the resistant and susceptible plants using specific primers for the coat protein (CP) and RNA-dependent RNA polymerase (RdRp) genes of BYDV-GAV serotype. On the inoculation site, the amount of the virus in the resistant wheat line (YW642) was much lower compared to the susceptible sib line (YW641). There was small amount of the virus could be detected in YW642 at 2-5 days post infestation (dpi), afterwards the amount of virus decreased and no virus could be detected at 14 and 16 dpi. In the uninoculated upper leaves, no BYDV was detected in YW642 from 1 to 14 dpi, while the virus could be detected at 3 dpi and then accumulated rapidly in YW641. These results showed at molecular level that the replication and/or movement of BYDV-GAV were strongly suppressed in YW642, presumably owing to the action of the BdV2 gene.","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 9","pages":"942-7"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25615047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Fine localization of rice EUI1 gene controlling elongation of the uppermost internode]. 水稻最上节间伸长控制基因EUI1的精细定位。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-09-01

Ren-Xiao Wang, Pei-Jin Li, Hong-Qi Chen, Shao-Kai Wen, Jia-Yang Li, Xu-Dong Zhu

引用次数: 0

[Functions of FANCL in primordial germ cell formation and Fanconi anemia]. [FANCL在原始生殖细胞形成和范可尼贫血中的作用]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-09-01

Qing-Guo Zhao, Bai-Song Lu, Pei-Tang Huang

{"title":"[Functions of FANCL in primordial germ cell formation and Fanconi anemia].","authors":"Qing-Guo Zhao, Bai-Song Lu, Pei-Tang Huang","doi":"","DOIUrl":"","url":null,"abstract":"Fanconi anemia (FA) is a rare autosomal recessive disorder characterized clinically by congenital abnormalities, progressive bone marrow failure and cancer susceptibility. Cells from individuals with Fanconi anemia manifest features of spontaneous chromosomal instability and hypersensitivity to DNA cross-linking agents such as mitomycin C. Over 11 known Fanconi anemia gene products are involved in DNA damage response pathway. In the pathway, monoubiquitination of FANCD2 is a key step. A novel protein FANCL is a component of the nuclear FA complex, functioned as an ubiquitin E3 ligase and monoubiquitinylated FANCD2. FANCD2-Ub is targeted to chromatin, where it interacts with BRCA2 to repair DNA damage. In early embryo stage, FA pathway is probably involved in proliferation of PGCs. Mice deficient in FA proteins, such as FANCL, FANCC and FANCA, have a drastic reduction of primordial germ cells (PGC), resulting in male and female infertility in adult. In the adult male, FANCL and a few testis-specific proteins, GGN1 (gametogenetin protein 1), GGNBP1 (gametogenetin binding protein 1), GGNBP2 and OAZ3 (ornithine decarboxylase antizyme 3) form a novel testis-specific complex functioning in spermatogenesis. FANCL is involved in proliferation of PGCs in early embryo stage, and development of germ cells in adult.","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 9","pages":"993-1000"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25615493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression pattern and preliminary functional analysis of human CREB4 gene. 人CREB4基因表达模式及初步功能分析。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-09-01

Yong-Juan Gao, Gen-Tao Cao, Gang Yin, Xin Wang, Chao-Neng Ji, Shao-Hua Gu, Xiao-Hua Ni

引用次数: 0

[QTLs mapping and genetic analysis of tiller angle in rice (Oryza sativa L.)]. 水稻分蘖角qtl定位与遗传分析[j]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-09-01

Chuan-Yuan Yu, Yu-Qiang Liu, Ling Jiang, Chun-Ming Wang, Hu-Qu Zhai, Jian-Min Wan

{"title":"[QTLs mapping and genetic analysis of tiller angle in rice (Oryza sativa L.)].","authors":"Chuan-Yuan Yu, Yu-Qiang Liu, Ling Jiang, Chun-Ming Wang, Hu-Qu Zhai, Jian-Min Wan","doi":"","DOIUrl":"","url":null,"abstract":"Tiller angle is one of the most important morphological characters that has a significant effect on the formation of rice high-yield population. The tiller angles were measured in a japonica/indica RIL population with 71 lines and a genome-wide chromosome segment substitution line (CSSL) population with 65 lines at two experimental sites. A transgressive segregation was observed in both populations. QTL analysis of tiller angle was conducted based on the saturated RFLP marker linkage map and the CSSL graphical genetype. Five main-effect QTLs and three pairs of epstatic loci were detected in the RIL population. A main QTL, qTA-9, located on chromosome 9 at XNpb108 - C506, was identified at both experiment sites. The positive allele TA-9(I) on qTA-9 was contributed by indica rice IR24 with 28.6% average contribution to variance. Meanwhile, an analysis of CSSL graphical genetypes also showed that there was a positive allele on the IR24 chromosome substitution segment delimited by RFLP marker C609 and C506 with approximate 15 cM interval, which proved the existence of qTA-9. The TA-9(I) could increase tiller angle by about 15 degrees in japonica Asominori background under the two environments. The measurement of the F1 from the cross between background parent and CSSL AIS68 with TA-9(I) and the analysis of F2 population indicated that the TA-9(I) was an incomplete dominant gene. Genetype x environment interaction(G xE) was not widely present except a pair of epistatic loci with 5.32% contribution to variance of tiller angles and a relatively small additive effect. The combining action of the additive effect of the genes from both parents and the two-loci epistasis-effect may be responsible for the transgressive segregation of tiller angle in rice population. The value and approach of application of TA-9(I) in hybrid rice breeding program were discussed.","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 9","pages":"948-54"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25615048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Identification of drought tolerant germplasm and inheritance and QTL mapping of related root traits in soybean (Glycine max (L.) Merr.)]. [大豆（Glycine max (L.) Merr.）耐旱种质的鉴定及相关根系性状的遗传和 QTL 绘图]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-08-01

Ying Liu, Jun-Yi Gai, Hui-Neng Lü, Yong-Jun Wang, Shou-Yi Chen

{"title":"[Identification of drought tolerant germplasm and inheritance and QTL mapping of related root traits in soybean (Glycine max (L.) Merr.)].","authors":"Ying Liu, Jun-Yi Gai, Hui-Neng Lü, Yong-Jun Wang, Shou-Yi Chen","doi":"","DOIUrl":"","url":null,"abstract":"Fifty nine accessions of soybean (Glycine max (L.) Merr.) selected from 301 ones in Huang-Huai-Hai and Middle-Lower Changjiang Valleys were tested in two years for their tolerance to drought by using the mean membership index value averaged over those of plant height,leave number,dry root weight and dry stem and leaf weight. Four most tolerant accessions (Rank 1) and two most sensitive ones (Rank 5) were identified. There existed very significant correlations between drought tolerance and relative values of dry root weight,total root length, and root volume (per plant dry weight basis), respectively,which could be used as root indicators of drought tolerance. The RIL population derived from Kefeng 1 x Nannong 1138-2 was used to analyze the inheritance of the three related root traits by using the segregation analysis of quantitative traits under the major gene plus polygene mixed inheritance model. The results showed that between the two parents (Rank 1 x Rank 4), the relative values of dry root weight,total root length and root volume were respectively controlled by two major genes (linked together for the latter two traits, recombination value being 4.30% and 1.93%, respectively) plus polygenes with their major gene heritability values of 62.26%-91.81% and polygene heritability values 2.99%-24.75%, indicating that the major genes,especially the one with larger effect,accounted for a major part of the genetic variation between the two parents. It was identified that five, three, and five QTLs located on N6-C2, N8-D1b + W, N11-E, and N18-K linkage groups for relative dry root weight, total root length and root volume, respectively. Each of the traits appeared to have one locus (Dw1, R/1, and Rv1) with relatively large effect in comparison with their other loci, and those major ones were located near the same site of the same linkage group N6-C2. The results of segregation analysis and QTL mapping appeared pretty consistent with each other, which could be used as a demonstration of each other.","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 8","pages":"855-63"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25653556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FISH analysis of pachytene chromosome and DNA fiber of telomere sequence in rice (Oryza sativa L. indica). 水稻粗线染色体和端粒序列DNA纤维的FISH分析。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-08-01

Zong-Yun Li, Rui Qin, Wei-Wei Jin, Zhi-Yong Xiong, Yun-Chun Song

引用次数: 0