Molecular evidence of barley yellow dwarf virus replication/movement suppressed by the resistance gene Bdv2.

Xiao-Dong Liu, Zeng-Yan Zhang, Yan Liu, Zhi-Yong Xin
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Abstract

A wheat-Thinopyrum intermedium translocation line YW642 possesses the resistance to GAV serotype of barley yellow dwarf virus (BYDV), in which the resistance gene Bdv2 is derived from the chromosome 7X of Thinopyrum intermedium group 7. It is interesting to analyze BYDV accumulation content in the resistant and susceptible wheat plants for controlling BYDV disease and understanding the resistance mechanism against BYDV. In the paper, semi-quantitative reverse-transcription PCR (RT-PCR) was used to detect and quantify BYDV-GAV in the resistant and susceptible plants using specific primers for the coat protein (CP) and RNA-dependent RNA polymerase (RdRp) genes of BYDV-GAV serotype. On the inoculation site, the amount of the virus in the resistant wheat line (YW642) was much lower compared to the susceptible sib line (YW641). There was small amount of the virus could be detected in YW642 at 2-5 days post infestation (dpi), afterwards the amount of virus decreased and no virus could be detected at 14 and 16 dpi. In the uninoculated upper leaves, no BYDV was detected in YW642 from 1 to 14 dpi, while the virus could be detected at 3 dpi and then accumulated rapidly in YW641. These results showed at molecular level that the replication and/or movement of BYDV-GAV were strongly suppressed in YW642, presumably owing to the action of the BdV2 gene.

抗基因Bdv2抑制大麦黄矮病毒复制/运动的分子证据
一株小麦-Thinopyrum中间易位系YW642对大麦黄矮病毒(BYDV) GAV血清型具有抗性,其中抗性基因Bdv2来源于Thinopyrum中间组7的7X染色体。分析小麦抗病和易感植株中BYDV的积累含量,对防治BYDV及了解其抗病机制具有重要意义。利用BYDV-GAV血清型外壳蛋白(CP)和RNA依赖RNA聚合酶(RdRp)基因的特异性引物,采用半定量反转录PCR (RT-PCR)技术对抗性和易感植物中的BYDV-GAV进行检测和定量。在接种现场,抗性小麦品系(YW642)的病毒量远低于易感同胞品系(YW641)。YW642侵染后2 ~ 5 d可检出少量病毒,侵染后病毒量下降,14、16 dpi时未检出病毒。在未接种的上叶中,YW642在1 ~ 14 dpi未检测到BYDV,而在3 dpi时可以检测到病毒,然后在YW641中迅速积累。这些结果表明,在分子水平上,BYDV-GAV的复制和/或运动在YW642中被强烈抑制,可能是由于BdV2基因的作用。
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